LOCUS       40924_3356        9194 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pAB34, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9194)
  AUTHORS   Bradley AS, Pearson A, Saenz JP, Marx CJ.
  TITLE     Adenosylhopane: the first step in biosynthesis of hopanoid side 
            chains
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9194)
  AUTHORS   Bradley AS, Pearson A, Saenz JP, Marx CJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-MAR-2010) OEB, Harvard University, 16 Divinity Ave., 
            Cambridge, MA 02138, USA
REFERENCE   3  (bases 1 to 9194)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9194)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-MAR-2010) OEB, Harvard University, 16 Divinity Ave., Cambridge, 
            MA 02138, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9194
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(4..861)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(862..966)
                     /label=AmpR promoter
     misc_feature    999..1538
                     /label=flank upstream of hpnG
                     /note="flank upstream of hpnG"
     misc_feature    1663..2173
                     /label=flank downstream of hpnG
                     /note="flank downstream of hpnG"
     CDS             complement(2809..3177)
                     /codon_start=1
                     /label=traJ
                     /note="oriT-recognizing protein"
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            complement(3210..3319)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     promoter        3956..3984
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     CDS             4032..5219
                     /codon_start=1
                     /label=TcR
                     /note="tetracycline efflux protein"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     CDS             complement(5403..6059)
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             complement(6160..7578)
                     /codon_start=1
                     /label=SacB
                     /note="secreted levansucrase that renders bacterial growth 
                     sensitive to sucrose"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     promoter        complement(7579..8024)
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     rep_origin      complement(8436..9024)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
       61 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
      121 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
      181 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
      241 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
      301 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
      361 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
      421 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
      481 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
      541 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
      601 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
      661 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
      721 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
      781 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
      841 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
      901 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
      961 tccgcgcaca tttccccgaa aagtgccacc tgacgtccca gaagcacttc ctcaaccgca
     1021 aggcgaccaa ggacctgttc cgcggcgtgt tccggcacgg caaggagaag ggccgcgaga
     1081 agtggacctt ccagcagtcg ggcctgttcc tcgacttcct cgccggcaac cagacctacc
     1141 attgcacccc gtggggcaac ccgacccgca ccgtgttcgg ctggcagaag ccctgctacc
     1201 tgctcggcga gggctacgcg gcgaccttca aggagctgat ggaagagacc gactgggatt
     1261 cctacggcac cggcaactac gagaagtgcg ccgactgcat ggtccactcg ggctacgagg
     1321 cgtcctcggt ggtcgattcg gtcaagaagc cgtggaagcc gctgatccac gcgatccgcg
     1381 gcatcaagac cgagggcgac atggcgccgg agatctccct ggagaaccag cgcccggccg
     1441 agttcgtgtt ctcccgcaac gtcgcccaga agctgtccga gatcaaagcc tcgggcgtcg
     1501 acaccaagca ggaagcccgc aagcgcaccg ccgcctgagc gacgcgcttt accgcttacc
     1561 atgagagacc cgcgcggcac cggccgcgcg ggtcttttca tttcaagggc gtcatatgct
     1621 gcagctcgag cggccgcggg cccgaagctc tgggtcagga acatgccttg cgcgtcgcgc
     1681 aggttcaccc ggtacaccac cggcacctgc cctgaggcca gcagcggcga gttcggccgc
     1741 agccgccgca ccaccgccgc cggctcgtag cggaagatga acacgcccgc cggatcggcc
     1801 tggaactcgc gcaggccgga ggcgcgtgcc agcgcctgcg acagcgtcag cccgtccgcc
     1861 gtgaacggca cctcggtggt gttgccgagc gcacccacgg ccaggaaggt ctgcggatcg
     1921 cgcaccaggg tcagcgtatc gttcggccgt acgaagatgt tctcgcgcgg gttggcgacc
     1981 accgtggtca tcggcacggt gacggtgcgg ttgccgcgcg agagccgcac gaaggtctcg
     2041 gccaccgggg tgcgcacgcc cccggcctgg gcgatgacgt cgagcagacg gtcgccgcgg
     2101 cccgacagcg gcacgcgcat gcccatggcg ccttcgccgg tgacggtggc cgattgtgag
     2161 atcggtttgg tgacggagac gagcacctgc ggctaccggt gagctcacta gaggatccag
     2221 ccgaccaggc tttccacgcc cgcgtgccgc tccatgtcgt tcgcgcggtt ctcggaaacg
     2281 cgctgccgcg tttcgtgatt gtcacgctca agcccgtagt cccgttcgag cgtcgcgcag
     2341 aggtcagcga gggcgcggta ggcccgatac ggctcatgga tggtgtttcg ggtcgggtga
     2401 atcttgttga tggcgatatg gatgtgcagg ttgtcggtgt cgtgatgcac ggcactgacg
     2461 cgctgatgct cggcgaagcc aagcccagcg cagatgcggt cctcaatcgc gcgcaacgtc
     2521 tccgcgtcgg gcttctctcc cgcgcggaag ctaaccagca ggtgataggt cttgtcggcc
     2581 tcggaacggg tgttgccgtg ctgggtcgcc atcacctcgg ccatgacagc gggcagggtg
     2641 tttgcctcgc agttcgtgac gcgcacgtga cccaggcgct cggtcttgcc ttgctcgtcg
     2701 gtgatgtact tcaccagctc cgcgaagtcg ctcttcttga tggagcgcat ggggacgtgc
     2761 ttggcaatca cgcgcacccc ccggccgttt tagcggctaa aaaagtcatg gctctgccct
     2821 cgggcggacc acgcccatca tgaccttgcc aagctcgtcc tgcttctctt cgatcttcgc
     2881 cagcagggcg aggatcgtgg catcaccgaa ccgcgccgtg cgcgggtcgt cggtgagcca
     2941 gagtttcagc aggccgccca ggcggcccag gtcgccattg atgcgggcca gctcgcggac
     3001 gtgctcatag tccacgacgc ccgtgatttt gtagccctgg ccgacggcca gcaggtaggc
     3061 cgacaggctc atgccggccg ccgccgcctt ttcctcaatc gctcttcgtt cgtctggaag
     3121 gcagtacacc ttgataggtg ggctgccctt cctggttggc ttggtttcat cagccatccg
     3181 cttgccctca tctgttacgc cggcggtagc cggccagcct cgcagagcag gattcccgtt
     3241 gagcaccgcc aggtgcgaat aagggacagt gaagaaggaa cacccgctcg cgggtgggcc
     3301 tacttcacct atcctgcccg gctgacgccg ttggatacac caaggaaagt ctacacgaac
     3361 cctttggcaa aatcctgtat atcgtgcgaa aaaggatgga tataccgaaa aaatcgctat
     3421 aatgaccccg aagcagggtt atgcagcgga aaagcgctgc ttccctgctg ttttgtggaa
     3481 tatctaccga ctggaaacag gcaaatgcag gaaattactg aactgagggg acaggcgaga
     3541 gacgatgcca aagagctaca ccgacgagct ggccgagtgg gttgaatccc gcgcggccaa
     3601 gaagcgccgg cgtgatgagg ctgcggttgc gttcctggcg gtgagggcgg atgtcgaggc
     3661 ggcgttagcg tccggctatg cgctcgtcac catttgggag cacatgcggg aaacggggaa
     3721 ggtcaagttc tcctacgaga cgttccgctc gcacgccagg cggcacatca aggccaagcc
     3781 cgccgatgtg cccgcaccgc aggccaaggc tgcggaaccc gcgccggcac ccaagacgcc
     3841 ggagccacgg cggccgaagc aggggggcaa ggctgaaaag ccggcccccg ctgcggcccc
     3901 gaccggcttc accttcaacc caacaccgga caaaaaggat ccccaattct catgtttgac
     3961 agcttatcat cgataagctt taatgcggta gtttatcaca gttaaattgc taacgcagtc
     4021 aggcaccgtg tatgaaatct aacaatgcgc tcatcgtcat cctcggcacc gtcaccctgg
     4081 atgctgtagg cataggcttg gttatgccgg tactgccggg cctcttgcgg gatatcgtcc
     4141 attccgacag catcgccagt cactatggcg tgctgctagc gctatatgcg ttgatgcaat
     4201 ttctatgcgc acccgttctc ggagcactgt ccgaccgctt tggccgccgc ccagtcctgc
     4261 tcgcttcgct acttggagcc actatcgact acgcgatcat ggcgaccaca cccgtcctgt
     4321 ggatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg
     4381 gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga
     4441 gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca
     4501 tctccttgca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg
     4561 gctgcttcct aatgcaggag tcgcataagg gagagcgtcg accgatgccc ttgagagcct
     4621 tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga
     4681 ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg gtcattttcg
     4741 gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg gtattcggaa
     4801 tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc caccaaacgt ttcggcgaga
     4861 agcaggccat tatcgccggc atggcggccg acgcgctggg ctacgtcttg ctggcgttcg
     4921 cgacgcgagg ctggatggcc ttccccatta tgattcttct cgcttccggc ggcatcggga
     4981 tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga cgaccatcag ggacagcttc
     5041 aaggatcgct cgcggctctt accagcctaa cttcgatcac tggaccgctg atcgtcacgg
     5101 cgatttatgc cgcctcggcg agcacatgga acgggttggc atggattgta ggcgccgccc
     5161 tataccttgt ctgcctcccc gcgttgcgtc gcggtgcatg gagccgggcc acctcgacct
     5221 gaatggaagc cggcggcacc tcgctaacgg attcaccact ccaagaattg gagccaatca
     5281 attcttgcgg agaactgtga atgcgcaaac caacccttgg cagaacatat ccatcgcgtc
     5341 cgccatctcc agcagccgca cgcggcgcat ctcgggcagc gttgggtcct ggccgggaat
     5401 tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca
     5461 tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg
     5521 ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc
     5581 acgtttaaat caaaactggt gaaactcacc cagggattgg ctgagacgaa aaacatattc
     5641 tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa
     5701 tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt
     5761 tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca
     5821 ccgtctttca ttgccatacg gaattccgga tgagcattca tcaggcgggc aagaatgtga
     5881 ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata
     5941 tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt
     6001 tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt
     6061 ttagcttcct tagctcctgc cctatgggat tcacctttat gttgataaga aataaaagaa
     6121 aatgccaata ggatatcggc attttctttt gcgtttttat ttgttaactg ttaattgtcc
     6181 ttgttcaagg atgctgtctt tgacaacaga tgttttcttg cctttgatgt tcagcaggaa
     6241 gcttggcgca aacgttgatt gtttgtctgc gtagaatcct ctgtttgtca tatagcttgt
     6301 aatcacgaca ttgtttcctt tcgcttgagg tacagcgaag tgtgagtaag taaaggttac
     6361 atcgttagga tcaagatcca tttttaacac aaggccagtt ttgttcagcg gcttgtatgg
     6421 gccagttaaa gaattagaaa cataaccaag catgtaaata tcgttagacg taatgccgtc
     6481 aatcgtcatt tttgatccgc gggagtcagt gaacaggtac catttgccgt tcattttaaa
     6541 gacgttcgcg cgttcaattt catctgttac tgtgttagat gcaatcagcg gtttcatcac
     6601 ttttttcagt gtgtaatcat cgtttagctc aatcataccg agagcgccgt ttgctaactc
     6661 agccgtgcgt tttttatcgc tttgcagaag tttttgactt tcttgacgga agaatgatgt
     6721 gcttttgcca tagtatgctt tgttaaataa agattcttcg ccttggtagc catcttcagt
     6781 tccagtgttt gcttcaaata ctaagtattt gtggccttta tcttctacgt agtgaggatc
     6841 tctcagcgta tggttgtcgc ctgagctgta gttgccttca tcgatgaact gctgtacatt
     6901 ttgatacgtt tttccgtcac cgtcaaagat tgatttataa tcctctacac cgttgatgtt
     6961 caaagagctg tctgatgctg atacgttaac ttgtgcagtt gtcagtgttt gtttgccgta
     7021 atgtttaccg gagaaatcag tgtagaataa acggattttt ccgtcagatg taaatgtggc
     7081 tgaacctgac cattcttgtg tttggtcttt taggatagaa tcatttgcat cgaatttgtc
     7141 gctgtcttta aagacgcggc cagcgttttt ccagctgtca atagaagttt cgccgacttt
     7201 ttgatagaac atgtaaatcg atgtgtcatc cgcattttta ggatctccgg ctaatgcaaa
     7261 gacgatgtgg tagccgtgat agtttgcgac agtgccgtca gcgttttgta atggccagct
     7321 gtcccaaacg tccaggcctt ttgcagaaga gatattttta attgtggacg aatcgaattc
     7381 aggaacttga tatttttcat ttttttgctg ttcagggatt tgcagcatat catggcgtgt
     7441 aatatgggaa atgccgtatg tttccttata tggcttttgg ttcgtttctt tcgcaaacgc
     7501 ttgagttgcg cctcctgcca gcagtgcggt agtaaaggtt aatactgttg cttgttttgc
     7561 aaactttttg atgttcatcg ttcatgtctc cttttttatg tactgtgtta gcggtctgct
     7621 tcttccagcc ctcctgtttg aagatggcaa gttagttacg cacaataaaa aaagacctaa
     7681 aatatgtaag gggtgacgcc aaagtataca ctttgccctt tacacatttt aggtcttgcc
     7741 tgctttatca gtaacaaacc cgcgcgattt acttttcgac ctcattctat tagactctcg
     7801 tttggattgc aactggtcta ttttcctctt ttgtttgata gaaaatcata aaaggatttg
     7861 cagactacgg gcctaaagaa ctaaaaaatc tatctgtttc ttttcattct ctgtattttt
     7921 tatagtttct gttgcatggg cataaagttg cctttttaat cacaattcag aaaatatcat
     7981 aatatctcat ttcactaaat aatagtgaac ggcaggtata tgtgatgggt taaaaaggat
     8041 cgatcctcta gccacgggtg cgcatgatcg tgctcctgtc gttgaggacc cggctaggct
     8101 ggcggggttg ccttactggt tagcagaatg aatcaccgat acgcgagcga acgtgaagcg
     8161 actgctgctg caaaacgtct gcgacctgag caacaacatg aatggtcttc ggtttccgtg
     8221 tttcgtaaag tctggaaacg cggaagtcag cgctcttccg cttcctcgct cactgactcg
     8281 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     8341 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     8401 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     8461 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     8521 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     8581 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     8641 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     8701 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     8761 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     8821 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
     8881 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     8941 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     9001 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     9061 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     9121 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     9181 acttggtctg acag
//
