LOCUS       40924_3351        9121 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pAB32, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9121)
  AUTHORS   Bradley AS, Pearson A, Saenz JP, Marx CJ.
  TITLE     Adenosylhopane: the first step in biosynthesis of hopanoid side 
            chains
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9121)
  AUTHORS   Bradley AS, Pearson A, Saenz JP, Marx CJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-MAR-2010) OEB, Harvard University, 16 Divinity Ave., 
            Cambridge, MA 02138, USA
REFERENCE   3  (bases 1 to 9121)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9121)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-MAR-2010) OEB, Harvard University, 16 Divinity Ave., Cambridge, 
            MA 02138, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9121
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    2..565
                     /label=flank upstream of hpnH
                     /note="flank upstream of hpnH"
     misc_feature    595..1116
                     /label=flank downstream of hpnH
                     /note="flank downstream of hpnH"
     regulatory      701..710
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(1740..2108)
                     /label=traJ
                     /note="oriT-recognizing protein"
     oriT            complement(2141..2250)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     promoter        2887..2915
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     CDS             2963..4150
                     /label=TcR
                     /note="tetracycline efflux protein"
     CDS             complement(4334..4990)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             complement(5091..6509)
                     /label=SacB
                     /note="secreted levansucrase that renders bacterial growth 
                     sensitive to sucrose"
     promoter        complement(6510..6955)
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     rep_origin      complement(7367..7955)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8129..8986)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(8987..9091)
                     /label=AmpR promoter
ORIGIN
        1 ccgcgaagag ctcgataaag actccacgcc gatcattcgt gtcgttggcg cgctacctac
       61 ttgtcccagc gggggaatgc aacgcggccc ggcaaaccgg tgcgatccgg cggcggcggt
      121 gcgattgcgc actgcaaccg gattgcgcga cgatatcgct tcgcacaagc attccgaacg
      181 atgtcggctt catgacgggg tagcggacgc cgcacgggac atcgtgcgag aaatcgtgaa
      241 cgtgtttcgc cccgtttcgc cccttgcggc ctgtccgtgc agaggaacgg tcaaaaatct
      301 gcggcagaat gcggaaatca aacgatcatt cccggttcgg atcgcgcgac caccggcttc
      361 atctccgaag ccgcagttcg gccgcgagac cggcacgaca cactctctcg aaatcgcgct
      421 caaagcgcgc cctctcggaa cagttccagc ctaatgcgct ggaacttctg tccttctcac
      481 gctaagcagc ccggctaagt ccggaacccg cgcatccaag gagccgcgtc ttgggaatcc
      541 ccatacggta cgtcgcgaag atccatatgc tgcagctcga gcggccgcgg gcccaccgct
      601 taccatgaga gacccgcgcg gcaccggccg cgcgggtctt ttcatttcaa gggcgtcgcg
      661 cgggaaaacg ctcgcctcac ggaaagcgga cgcgctgcag ccatggtggc ggctgagcgc
      721 ccctcgcgga acggccgtcg cggaagaggg aagggcgggc gcctcgcgag gcgatcttat
      781 agcagcccga gacccggtcc gagccggacc cggcagcggg cgagcgccgc gaaggccgcc
      841 gaggaatcgc ggccgagccg aatcatctcg ccgatccggg cgcggccgcg cagcagcgcg
      901 ccgagcaccg cccgaatgtc cggttcgccc tccggcgtca gggccgaggc cgcgaaggcg
      961 ggcagggcgc gtcctgcggg gtcgcaaacc acccggatcg ccgcgaaggg gagggcatgg
     1021 cgggcggcgt aggcggcggc cacatgcgat tccatatcga cggccagggc gccggtgcgc
     1081 gcatgcaaag ccgccttatc ggcaacggtc atgacacgcg tgttaaccgg tgagctcact
     1141 agaggatcca gccgaccagg ctttccacgc ccgcgtgccg ctccatgtcg ttcgcgcggt
     1201 tctcggaaac gcgctgccgc gtttcgtgat tgtcacgctc aagcccgtag tcccgttcga
     1261 gcgtcgcgca gaggtcagcg agggcgcggt aggcccgata cggctcatgg atggtgtttc
     1321 gggtcgggtg aatcttgttg atggcgatat ggatgtgcag gttgtcggtg tcgtgatgca
     1381 cggcactgac gcgctgatgc tcggcgaagc caagcccagc gcagatgcgg tcctcaatcg
     1441 cgcgcaacgt ctccgcgtcg ggcttctctc ccgcgcggaa gctaaccagc aggtgatagg
     1501 tcttgtcggc ctcggaacgg gtgttgccgt gctgggtcgc catcacctcg gccatgacag
     1561 cgggcagggt gtttgcctcg cagttcgtga cgcgcacgtg acccaggcgc tcggtcttgc
     1621 cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg atggagcgca
     1681 tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta aaaaagtcat
     1741 ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc ctgcttctct
     1801 tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt gcgcgggtcg
     1861 tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt gatgcgggcc
     1921 agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg gccgacggcc
     1981 agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat cgctcttcgt
     2041 tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg cttggtttca
     2101 tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc tcgcagagca
     2161 ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga acacccgctc
     2221 gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca ccaaggaaag
     2281 tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg atataccgaa
     2341 aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgctg cttccctgct
     2401 gttttgtgga atatctaccg actggaaaca ggcaaatgca ggaaattact gaactgaggg
     2461 gacaggcgag agacgatgcc aaagagctac accgacgagc tggccgagtg ggttgaatcc
     2521 cgcgcggcca agaagcgccg gcgtgatgag gctgcggttg cgttcctggc ggtgagggcg
     2581 gatgtcgagg cggcgttagc gtccggctat gcgctcgtca ccatttggga gcacatgcgg
     2641 gaaacgggga aggtcaagtt ctcctacgag acgttccgct cgcacgccag gcggcacatc
     2701 aaggccaagc ccgccgatgt gcccgcaccg caggccaagg ctgcggaacc cgcgccggca
     2761 cccaagacgc cggagccacg gcggccgaag caggggggca aggctgaaaa gccggccccc
     2821 gctgcggccc cgaccggctt caccttcaac ccaacaccgg acaaaaagga tccccaattc
     2881 tcatgtttga cagcttatca tcgataagct ttaatgcggt agtttatcac agttaaattg
     2941 ctaacgcagt caggcaccgt gtatgaaatc taacaatgcg ctcatcgtca tcctcggcac
     3001 cgtcaccctg gatgctgtag gcataggctt ggttatgccg gtactgccgg gcctcttgcg
     3061 ggatatcgtc cattccgaca gcatcgccag tcactatggc gtgctgctag cgctatatgc
     3121 gttgatgcaa tttctatgcg cacccgttct cggagcactg tccgaccgct ttggccgccg
     3181 cccagtcctg ctcgcttcgc tacttggagc cactatcgac tacgcgatca tggcgaccac
     3241 acccgtcctg tggatcctct acgccggacg catcgtggcc ggcatcaccg gcgccacagg
     3301 tgcggttgct ggcgcctata tcgccgacat caccgatggg gaagatcggg ctcgccactt
     3361 cgggctcatg agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg ccgggggact
     3421 gttgggcgcc atctccttgc atgcaccatt ccttgcggcg gcggtgctca acggcctcaa
     3481 cctactactg ggctgcttcc taatgcagga gtcgcataag ggagagcgtc gaccgatgcc
     3541 cttgagagcc ttcaacccag tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc
     3601 cgcacttatg actgtcttct ttatcatgca actcgtagga caggtgccgg cagcgctctg
     3661 ggtcattttc ggcgaggacc gctttcgctg gagcgcgacg atgatcggcc tgtcgcttgc
     3721 ggtattcgga atcttgcacg ccctcgctca agccttcgtc actggtcccg ccaccaaacg
     3781 tttcggcgag aagcaggcca ttatcgccgg catggcggcc gacgcgctgg gctacgtctt
     3841 gctggcgttc gcgacgcgag gctggatggc cttccccatt atgattcttc tcgcttccgg
     3901 cggcatcggg atgcccgcgt tgcaggccat gctgtccagg caggtagatg acgaccatca
     3961 gggacagctt caaggatcgc tcgcggctct taccagccta acttcgatca ctggaccgct
     4021 gatcgtcacg gcgatttatg ccgcctcggc gagcacatgg aacgggttgg catggattgt
     4081 aggcgccgcc ctataccttg tctgcctccc cgcgttgcgt cgcggtgcat ggagccgggc
     4141 cacctcgacc tgaatggaag ccggcggcac ctcgctaacg gattcaccac tccaagaatt
     4201 ggagccaatc aattcttgcg gagaactgtg aatgcgcaaa ccaacccttg gcagaacata
     4261 tccatcgcgt ccgccatctc cagcagccgc acgcggcgca tctcgggcag cgttgggtcc
     4321 tggccgggaa ttacgccccg ccctgccact catcgcagta ctgttgtaat tcattaagca
     4381 ttctgccgac atggaagcca tcacaaacgg catgatgaac ctgaatcgcc agcggcatca
     4441 gcaccttgtc gccttgcgta taatatttgc ccatggtgaa aacgggggcg aagaagttgt
     4501 ccatattggc cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgagacga
     4561 aaaacatatt ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca
     4621 catcttgcga atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagcg
     4681 atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata
     4741 tcaccagctc accgtctttc attgccatac ggaattccgg atgagcattc atcaggcggg
     4801 caagaatgtg aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa
     4861 aggccgtaat atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg
     4921 cctcaaaatg ttctttacga tgccattggg atatatcaac ggtggtatat ccagtgattt
     4981 ttttctccat tttagcttcc ttagctcctg ccctatggga ttcaccttta tgttgataag
     5041 aaataaaaga aaatgccaat aggatatcgg cattttcttt tgcgttttta tttgttaact
     5101 gttaattgtc cttgttcaag gatgctgtct ttgacaacag atgttttctt gcctttgatg
     5161 ttcagcagga agcttggcgc aaacgttgat tgtttgtctg cgtagaatcc tctgtttgtc
     5221 atatagcttg taatcacgac attgtttcct ttcgcttgag gtacagcgaa gtgtgagtaa
     5281 gtaaaggtta catcgttagg atcaagatcc atttttaaca caaggccagt tttgttcagc
     5341 ggcttgtatg ggccagttaa agaattagaa acataaccaa gcatgtaaat atcgttagac
     5401 gtaatgccgt caatcgtcat ttttgatccg cgggagtcag tgaacaggta ccatttgccg
     5461 ttcattttaa agacgttcgc gcgttcaatt tcatctgtta ctgtgttaga tgcaatcagc
     5521 ggtttcatca cttttttcag tgtgtaatca tcgtttagct caatcatacc gagagcgccg
     5581 tttgctaact cagccgtgcg ttttttatcg ctttgcagaa gtttttgact ttcttgacgg
     5641 aagaatgatg tgcttttgcc atagtatgct ttgttaaata aagattcttc gccttggtag
     5701 ccatcttcag ttccagtgtt tgcttcaaat actaagtatt tgtggccttt atcttctacg
     5761 tagtgaggat ctctcagcgt atggttgtcg cctgagctgt agttgccttc atcgatgaac
     5821 tgctgtacat tttgatacgt ttttccgtca ccgtcaaaga ttgatttata atcctctaca
     5881 ccgttgatgt tcaaagagct gtctgatgct gatacgttaa cttgtgcagt tgtcagtgtt
     5941 tgtttgccgt aatgtttacc ggagaaatca gtgtagaata aacggatttt tccgtcagat
     6001 gtaaatgtgg ctgaacctga ccattcttgt gtttggtctt ttaggataga atcatttgca
     6061 tcgaatttgt cgctgtcttt aaagacgcgg ccagcgtttt tccagctgtc aatagaagtt
     6121 tcgccgactt tttgatagaa catgtaaatc gatgtgtcat ccgcattttt aggatctccg
     6181 gctaatgcaa agacgatgtg gtagccgtga tagtttgcga cagtgccgtc agcgttttgt
     6241 aatggccagc tgtcccaaac gtccaggcct tttgcagaag agatattttt aattgtggac
     6301 gaatcgaatt caggaacttg atatttttca tttttttgct gttcagggat ttgcagcata
     6361 tcatggcgtg taatatggga aatgccgtat gtttccttat atggcttttg gttcgtttct
     6421 ttcgcaaacg cttgagttgc gcctcctgcc agcagtgcgg tagtaaaggt taatactgtt
     6481 gcttgttttg caaacttttt gatgttcatc gttcatgtct ccttttttat gtactgtgtt
     6541 agcggtctgc ttcttccagc cctcctgttt gaagatggca agttagttac gcacaataaa
     6601 aaaagaccta aaatatgtaa ggggtgacgc caaagtatac actttgccct ttacacattt
     6661 taggtcttgc ctgctttatc agtaacaaac ccgcgcgatt tacttttcga cctcattcta
     6721 ttagactctc gtttggattg caactggtct attttcctct tttgtttgat agaaaatcat
     6781 aaaaggattt gcagactacg ggcctaaaga actaaaaaat ctatctgttt cttttcattc
     6841 tctgtatttt ttatagtttc tgttgcatgg gcataaagtt gcctttttaa tcacaattca
     6901 gaaaatatca taatatctca tttcactaaa taatagtgaa cggcaggtat atgtgatggg
     6961 ttaaaaagga tcgatcctct agccacgggt gcgcatgatc gtgctcctgt cgttgaggac
     7021 ccggctaggc tggcggggtt gccttactgg ttagcagaat gaatcaccga tacgcgagcg
     7081 aacgtgaagc gactgctgct gcaaaacgtc tgcgacctga gcaacaacat gaatggtctt
     7141 cggtttccgt gtttcgtaaa gtctggaaac gcggaagtca gcgctcttcc gcttcctcgc
     7201 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     7261 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     7321 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     7381 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     7441 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     7501 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     7561 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     7621 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     7681 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     7741 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     7801 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     7861 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     7921 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     7981 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     8041 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     8101 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     8161 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     8221 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     8281 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     8341 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     8401 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     8461 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     8521 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     8581 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     8641 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     8701 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     8761 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     8821 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     8881 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     8941 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     9001 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     9061 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     9121 t
//
