LOCUS       V009656                 6566 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009656
VERSION     V009656
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6566)
  AUTHORS   Dennehy ME, Bourn W, Steele AD, Williamson A-L.
  TITLE     Recombinant BCG expressing rotavirus VP6 partially protects mice
            from rotavirus challenge
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6566)
  AUTHORS   Dennehy ME.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUL-2005) Medical Virology, University of Cape Town,
            Anzio Road, Observatory, Cape Town 7925, South Africa
REFERENCE   3  (bases 1 to 6566)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6566)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (03-JUL-2005) Medical Virology, University of Cape Town, Anzio Road,
            Observatory, Cape Town 7925, South Africa"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6566
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             121..933
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      1268..1856
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     rep_origin      2029..3927
                     /label="Mycobacterial OriM region derived from pAL5000"
                     /note="Mycobacterial OriM region derived from pAL5000"
     regulatory      3957..4197
                     /note="derived from Mycobacterium bovis hsp60 promoter of
                     GenBank Accession Number M17705"
                     /regulatory_class="promoter"
     regulatory      4240..4244
                     /label="alpha antigen Shine-Dalgarno sequence"
                     /note="alpha antigen Shine-Dalgarno sequence"
                     /regulatory_class="ribosome_binding_site"
     CDS             4254..5225
                     /gene="fbpB"
                     /label="Diacylglycerol acyltransferase/mycolyltransferase
                     Ag85B"
                     /note="Diacylglycerol acyltransferase/mycolyltransferase
                     Ag85B from Mycobacterium bovis (strain BCG / Pasteur
                     1173P2). Accession#: A1KJU9"
     CDS             5235..6425
                     /note="Intermediate capsid protein VP6 from Rotavirus A
                     (strain RVA/Monkey/United States/RRV/1975/G3P5B[3]).
                     Accession#: B2BN53"
                     /label="Intermediate capsid protein VP6"
     terminator      6484..6530
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 gctagccaac aaagcgacgt tgtgtctcaa aatctctgat gttacattgc acaagataaa
       61 aatatatcat catgaacaat aaaactgtct gcttacataa acagtaatac aaggggtgtt
      121 atgagccata ttcaacggga aacgtcttgc tcgaggccgc gattaaattc caacatggat
      181 gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc
      241 tatcgcttgt atgggaagcc ccatgcgcca gagttgtttc tgaaacatgg caaaggtagc
      301 gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct
      361 cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg
      421 atccccggga aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt
      481 gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct
      541 tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg
      601 gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa
      661 gaaatgcata atcttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca
      721 cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc
      781 ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct
      841 ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa
      901 ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa ttggttgtaa
      961 cactggcaga gcattacgct gacttgacgg gacggcggct ttgttgaata aatcgaactt
     1021 ttgctgagtt gaaggatcag atcacgcatc ttcccgacaa cgcagaccgt tccgtggcaa
     1081 agcaaaagtt caaaatcacc aactggtcca cctacaacaa agctctcatc aaccgtggct
     1141 ccctcacttt ctggctggat gatggggcga ttcaggcctg gtatgagtca gcaacacctt
     1201 cttcacgagg cagacctcac tagttccact gagcgtcaga ccccgtagaa aagatcaaag
     1261 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     1321 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     1381 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     1441 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     1501 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     1561 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     1621 gaacgaccta caccgaactg agatacctac agcgtgagca ttgagaaagc gccacgcttc
     1681 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     1741 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     1801 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     1861 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     1921 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     1981 ccgctcgccg cagccgaacg accgagcgca acgcgtgcgg ccgcacgcgt gagcccacca
     2041 gctccgtaag ttcgggcgct gtgtggctcg tacccgcgca ttcaggcggc agggggtcta
     2101 acgggtctaa ggcggcgtgt acggccgcca cagcggctct cagcggcccg gaaacgtcct
     2161 cgaaacgacg catgtgttcc tcctggttgg tacaggtggt tgggggtgct cggctgtcgc
     2221 tggtgttcca ccaccagggc tcgacgggag agcgggggag tgtgcagttg tggggtggcc
     2281 cctcagcgaa atatctgact tggagctcgt gtcggaccat acaccggtga ttaatcgtgg
     2341 tctactacca agcgtgagcc acgtcgccga cgaatttgag cagctctggc tgccgtactg
     2401 gccgctggca agcgacgatc tgctcgaggg gatctaccgc caaagccgcg cgtcggccct
     2461 aggccgccgg tacatcgagg cgaacccaac agcgctggca aacctgctgg tcgtggacgt
     2521 agaccatcca gacgcagcgc tccgagcgct cagcgcccgg gggtcccatc cgctgcccaa
     2581 cgcgatcgtg ggcaatcgcg ccaacggcca cgcacacgca gtgtgggcac tcaacgcccc
     2641 tgttccacgc accgaatacg cgcggcgtaa gccgctcgca tacatggcgg cgtgcgccga
     2701 aggccttcgg cgcgccgtcg atggcgaccg cagttactca ggcctcatga ccaaaaaccc
     2761 cggccacatc gcctgggaaa cggaatggct ccactcagat ctctacacac tcagccacat
     2821 cgaggccgag ctcggcgcga acatgccacc gccgcgctgg cgtcagcaga ccacgtacaa
     2881 agcggctccg acgccgctag ggcggaattg cgcactgttc gattccgtca ggttgtgggc
     2941 ctatcgtccc gccctcatgc ggatctacct gccgacccgg aacgtggacg gactcggccg
     3001 cgcgatctat gccgagtgcc acgcgcgaaa cgccgaattt ccgtgcaacg acgtgtgtcc
     3061 cggaccgcta ccggacagcg aggtccgcgc catcgccaac agcatttggc gttggatcac
     3121 aaccaagtcg cgcatttggg cggacgggat cgtggtctac gaggccacac tcagtgcgcg
     3181 ccagtcggcc atctcgcgga agggcgcagc agcgcgcacg gcggcgagca cagttgcgcg
     3241 gcgcgcaaag tccgcgtcag ccatgcatgg aggcattgct atgagcgacg gctacagcga
     3301 cggctacagc gacggctaca accggcagcc gactgtccgc aaaaagcggc gcgtgaccgc
     3361 cgccgaaggc gctcgaatca ccggactatc cgaacgccac gtcgtccggc tcgtggcgca
     3421 ggaacgcagc gagtggctcg ccgagcaggc tgcacgccgc gaacgcatcc gcgcctatca
     3481 cgacgacgag ggccactctt ggccgcaaac ggccaaacat ttcgggctgc atctggacac
     3541 cgttaagcga ctcggctatc gggcgaggaa agagcgtgcg gcagaacagg aagcggctca
     3601 aaaggcccac aacgaagccg acaatccacc gctgttctaa cgcaattggg gagcgggtgt
     3661 cgcgggggtt ccgtgggggg ttccgttgca acgggtcgga caggtaaaag tcctggtaga
     3721 cgctagtttt ctggtttggg ccatgcctgt ctcgttgcgt gtttcgttgc gcccgttttg
     3781 aataccagcc agacgagacg gggttctacg aatcttggtc gataccaagc catttccgct
     3841 gaatatcggg gagctcaccg ccagaatcgg tggttgtggt gatgtacgtg gcgaactccg
     3901 ttgtagtgtt gtggtggcat ccgtggcgcg gccgcggtac cagatcttta aatctagaca
     3961 aggtcgaacg aggggcatga cccggtgcgg ggcttcttgc actcggcata ggcgagtgct
     4021 aagaataacg ttggcactcg cgaccggtga gtcgtaggtc gggacggtga ggccaggccc
     4081 gtcgtcgcag cgagtggcag cgaggacaac ttgagccgtc cgtcgcgggc actgcgcccg
     4141 gccagcgtaa gtagcggggt tgccgtcacc cggtgacccc cggtttcatc cccgatcgct
     4201 agatatccat ggatctgagc acacgacgac atacaggaca aaggggcaca ggtatgacag
     4261 acgtgagccg aaagattcga gcttggggac gccgattgat gatcggcacg gcagcggctg
     4321 tagtccttcc gggcctggtg gggcttgccg gcggagcggc aaccgcgggc gcgttctccc
     4381 ggccggggct gccggtcgag tacctgcagg tgccgtcgcc gtcgatgggc cgcgacatca
     4441 aggttcagtt ccagagcggt gggaacaact cacctgcggt ttatctgctc gacggcctgc
     4501 gcgcccaaga cgactacaac ggctgggata tcaacacccc ggcgttcgag tggtactacc
     4561 agtcgggact gtcgatagtc atgccggtcg gcgggcagtc cagcttctac agcgactggt
     4621 acagcccggc ctgcggtaag gctggctgcc agacttacaa gtgggaaacc ctcctgacca
     4681 gcgagctgcc gcaatggttg tccgccaaca gggccgtgaa gcccaccggc agcgctgcaa
     4741 tcggcttgtc gatggccggc tcgtcggcaa tgatcttggc cgcctaccac ccccagcagt
     4801 tcatctacgc cggctcgctg tcggccctgc tggacccctc tcaggggatg gggcctagcc
     4861 tgatcggcct cgcgatgggt gacgccggcg gttacaaggc cgcagacatg tggggtccct
     4921 cgagtgaccc ggcatgggag cgcaacgacc ctacgcagca gatccccaag ctggtcgcaa
     4981 acaacacccg gctatgggtt tattgcggga acggcacccc gaacgagttg ggcggtgcca
     5041 acatacccgc cgagttcttg gagaacttcg ttcgtagcag caacctgaag ttccaggatg
     5101 cgtacaagcc cgcgggcggg cacaacgccg tgttcaactt cccgcccaac ggcacgcaca
     5161 gctgggagta ctggggcgct cagctcaacg ccatgaaggg tgacctgcag agttcgttag
     5221 gcgcggatcc caaaatggat gtgctgtact ccatctcgag aacactgaag gatgctaggg
     5281 ataaaattgt tgagggtacg ttgtactcta acgttagtga tcttattcag cagttcaatc
     5341 agatgctggt cacaatgaat ggaaatgaat ttcaaacggg gggaattggt aacttgccac
     5401 ttcgtaattg gaactttgat tttggactac taggtactac gctattaaat ctagacgcaa
     5461 actacgttga atcagccaga accacaattg attattttgt tgatttcata gataatgtat
     5521 gtatggatga aatggtgcga gagtcgcaaa ggaatggaat agcaccacaa tcagatgcct
     5581 tgcggaagtt atcaggagtt aagttcagac gaataaactt taacaattca tcagagtata
     5641 ttgagaactg gaaccttcag aaccgcaggc aaaggacggg attcacgttt cacaaaccga
     5701 acatatttcc atattcggca tcattcacat tgaataggtc gcagccgcag catgacaact
     5761 taatgggcac aatgtggcta aacgctgggt cagaaataca ggttgcagga tttgactatt
     5821 catgcgcaat taacgctcca gctaacattc aacaatttga acatatagtg caattacgga
     5881 gggtattgac tactgcaacc attacgctac ttccggatgc agagaggttt agtttcccaa
     5941 gagttattaa ctcagcagac ggagcaacta cgtggtattt caacccagta atattgcgtc
     6001 caaataatgt agaagttgaa ttcttattga atggtcagat catcaatact taccaagcta
     6061 gattcggtac catcgtcgca agaaattttg ataccatacg cctttctttt cagctgatga
     6121 gaccaccaaa catgacacca gcagtggctg cacttttccc gaatgctcaa ccgttcgagc
     6181 atcatgcaac agtcggactg acgcttagga ttgactcagc aatttgtgaa tcagttcttg
     6241 cagacgcaag tgaaactatg ttggctaatg tgacgtcagt cagacaagaa tacgcaatac
     6301 cagtaggacc agtatttcca ccaggaatga attggaccga cctcattact aattattcac
     6361 catcaaggga ggacaatctg caacgtgtgt ttacagtagc ttccattaga agcatgttgg
     6421 taaagtgagc gaagcttatc gatgtcgacg tagttaacta gcgtacgatc gactgccagg
     6481 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
     6541 tccggccatc atggccgcgg tgatca
//
