LOCUS       V011700                 9783 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V011700
VERSION     V011700
KEYWORDS    pAB-bee-FH
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9783)
  AUTHORS   AB Vector
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 9783)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9783
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             76..579
                     /gene="PTP"
                     /label="Tyrosine-protein phosphatase"
                     /note="Tyrosine-protein phosphatase from Autographa
                     californica nuclear polyhedrosis virus. Accession#: P24656"
     CDS             2662..3291
                     /gene="LEF-2"
                     /label="Primase-associated factor LEF-2"
                     /note="Primase-associated factor LEF-2 from Autographa
                     californica nuclear polyhedrosis virus. Accession#: P41418"
     misc_recomb     3170..3997
                     /label="baculovirus recombination region (lef2/ORF603)"
                     /note="contains ORF603 and part of lef2"
     promoter        4001..4092
                     /label="polyhedrin promoter"
                     /note="promoter for the baculovirus polyhedrin gene"
     sig_peptide     4143..4205
                     /label="melittin signal sequence"
                     /note="signal sequence from honeybee melittin"
     misc_feature    4203..4237
                     /label="MCS"
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             4236..4259
                     /label="FLAG"
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             4269..4292
                     /label="8xHis"
                     /note="8xHis affinity tag"
     misc_recomb     4798..6253
                     /label="baculovirus recombination region (ORF1629)"
                     /note="contains part of ORF1629"
     primer_bind     complement(7164..7180)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(7188..7204)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7212..7242)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7257..7278)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7566..8154)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8328..9185)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(9186..9290)
                     /label="AmpR promoter"
     primer_bind     9764..9780
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
ORIGIN
        1 aagctttact cgtaaagcga gttgaaggat catatttagt tgcgtttatg agataagatt
       61 gaaagcacgt gtaaaatgtt tcccgcgcgt tggcacaact atttacaatg cggccaagtt
      121 ataaaagatt ctaatctgat atgttttaaa acacctttgc ggcccgagtt gtttgcgtac
      181 gtgactagcg aagaagatgt gtggaccgca gaacagatag taaaacaaaa ccctagtatt
      241 ggagcaataa tcgatttaac caacacgtct aaatattatg atggtgtgca ttttttgcgg
      301 gcgggcctgt tatacaaaaa aattcaagta cctggccaga ctttgccgcc tgaaagcata
      361 gttcaagaat ttattgacac ggtaaaagaa tttacagaaa agtgtcccgg catgttggtg
      421 ggcgtgcact gcacacacgg tattaatcgc accggttaca tggtgtgcag atatttaatg
      481 cacaccctgg gtattgcgcc gcaggaagcc atagatagat tcgaaaaagc cagaggtcac
      541 aaaattgaaa gacaaaatta cgttcaagat ttattaattt aattaatatt atttgcattc
      601 tttaacaaat actttatcct attttcaaat tgttgcgctt cttccagcga accaaaacta
      661 tgcttcgctt gctccgttta gcttgtagcc gatcagtggc gttgttccaa tcgacggtag
      721 gattaggccg gatattctcc accacaatgt tggcaacgtt gatgttacgt ttatgctttt
      781 ggttttccac gtacgtcttt tggccggtaa tagccgtaaa cgtagtgccg tcgcgcgtca
      841 cgcacaacac cggatgtttg cgcttgtccg cggggtattg aaccgcgcga tccgacaaat
      901 ccaccacttt ggcaactaaa tcggtgacct gcgcgtcttt tttctgcatt atttcgtctt
      961 tcttttgcat ggtttcctgg aagccggtgt acatgcggtt tagatcagtc atgacgcgcg
     1021 tgacctgcaa atctttggcc tcgatctgct tgtccttgat ggcaacgatg cgttcaataa
     1081 actcttgttt tttaacaagt tcctcggttt tttgcgccac caccgcttgc agcgcgtttg
     1141 tgtgctcggt gaatgtcgca atcagcttag tcaccaactg tttgctctcc tcctcccgtt
     1201 gtttgatcgc gggatcgtac ttgccggtgc agagcacttg aggaattact tcttctaaaa
     1261 gccattcttg taattctatg gcgtaaggca atttggactt cataatcagc tgaatcacgc
     1321 cggatttagt aatgagcact gtatgcggct gcaaatacag cgggtcgccc cttttcacga
     1381 cgctgttaga ggtagggccc ccattttgga tggtctgctc aaataacgat ttgtatttat
     1441 tgtctacatg aacacgtata gctttatcac aaactgtata ttttaaactg ttagcgacgt
     1501 ccttggccac gaaccggacc tgttggtcgc gctctagcac gtaccgcagg ttgaacgtat
     1561 cttctccaaa tttaaattct ccaattttaa cgcgagccat tttgatacac gtgtgtcgat
     1621 tttgcaacaa ctattgtttt ttaacgcaaa ctaaacttat tgtggtaagc aataattaaa
     1681 tatgggggaa catgcgccgc tacaacactc gtcgttatga acgcagacgg cgccggtctc
     1741 ggcgcaagcg gctaaaacgt gttgcgcgtt caacgcggca aacatcgcaa aagccaatag
     1801 tacagttttg atttgcatat taacggcgat tttttaaatt atcttattta ataaatagtt
     1861 atgacgccta caactccccg cccgcgttga ctcgctgcac ctcgagcagt tcgttgacgc
     1921 cttcctccgt gtggccgaac acgtcgagcg ggtggtcgat gaccagcggc gtgccgcacg
     1981 cgacgcacaa gtatctgtac accgaatgat cgtcgggcga aggcacgtcg gcctccaagt
     2041 ggcaatattg gcaaattcga aaatatatac agttgggttg tttgcgcata tctatcgtgg
     2101 cgttgggcat gtacgtccga acgttgattt gcatgcaagc cgaaattaaa tcattgcgat
     2161 tagtgcgatt aaaacgttgt acatcctcgc ttttaatcat gccgtcgatt aaatcgcgca
     2221 atcgagtcaa gtgatcaaag tgtggaataa tgttttcttt gtattcccga gtcaagcgca
     2281 gcgcgtattt taacaaacta gccatcttgt aagttagttt catttaatgc aactttatcc
     2341 aataatatat tatgtatcgc acgtcaagaa ttaacaatgc gcccgttgtc gcatctcaac
     2401 acgactatga tagagatcaa ataaagcgcg aattaaatag cttgcgacgc aacgtgcacg
     2461 atctgtgcac gcgttccggc acgagctttg attgtaataa gtttttacga agcgatgaca
     2521 tgacccccgt agtgacaacg atcacgccca aaagaactgc cgactacaaa attaccgagt
     2581 atgtcggtga cgttaaaact attaagccat ccaatcgacc gttagtcgaa tcaggaccgc
     2641 tggtgcgaga agccgcgaag tatggcgaat gcatcgtata acgtgtggag tccgctcatt
     2701 agagcgtcat gtttagacaa gaaagctaca tatttaattg atcccgatga ttttattgat
     2761 aaattgaccc taactccata cacggtattc tacaatggcg gggttttggt caaaatttcc
     2821 ggactgcgat tgtacatgct gttaacggct ccgcccacta ttaatgaaat taaaaattcc
     2881 aattttaaaa aacgcagcaa gagaaacatt tgtatgaaag aatgcgtaga aggaaagaaa
     2941 aatgtcgtcg acatgctgaa caacaagatt aatatgcctc cgtgtataaa aaaaatattg
     3001 aacgatttga aagaaaacaa tgtaccgcgc ggcggtatgt acaggaagag gtttatacta
     3061 aactgttaca ttgcaaacgt ggtttcgtgt gccaagtgtg aaaaccgatg tttaatcaag
     3121 gctctgacgc atttctacaa ccacgactcc aagtgtgtgg gtgaagtcat gcatctttta
     3181 atcaaatccc aagatgtgta taaaccacca aactgccaaa aaatgaaaac tgtcgacaag
     3241 ctctgtccgt ttgctggcaa ctgcaagggt ctcaatccta tttgtaatta ttgaataata
     3301 aaacaattat aaatgctaaa tttgtttttt attaacgata caaaccaaac gcaacaagaa
     3361 catttgtagt attatctata attgaaaacg cgtagttata atcgctgagg taatatttaa
     3421 aatcattttc aaatgattca cagttaattt gcgacaatat aattttattt tcacataaac
     3481 tagacgcctt gtcgtcttct tcttcgtatt ccttctcttt ttcatttttc tcctcataaa
     3541 aattaacata gttattatcg tatccatata tgtatctatc gtatagagta aattttttgt
     3601 tgtcataaat atatatgtct tttttaatgg ggtgtatagt accgctgcgc atagtttttc
     3661 tgtaatttac aacagtgcta ttttctggta gttcttcgga gtgtgttgct ttaattatta
     3721 aatttatata atcaatgaat ttgggatcgt cggttttgta caatatgttg ccggcatagt
     3781 acgcagcttc ttctagttca attacaccat tttttagcag caccggatta acataacttt
     3841 ccaaaatgtt gtacgaaccg ttaaacaaaa acagttcacc tcccttttct atactattgt
     3901 ctgcgagcag ttgtttgttg ttaaaaataa cagccattgt aatgagacgc acaaactaat
     3961 atcacaaact ggaaatgtct atcaatatat agttgctgat atcatggaga taattaaaat
     4021 gataaccatc tcgcaaataa ataagtattt tactgttttc gtaacagttt tgtaataaaa
     4081 aaacctataa atattccgga ttattcatac cgtcccacca tcgggcgcgg atcagatcta
     4141 aaatgaaatt cttagtcaac gttgcccttg tttttatggt cgtatacatt tcttacatct
     4201 atgcggccgc tccggaattc ccgggatccg gtctagatta taaggatgac gacgataaat
     4261 tcgtcgagca ccaccaccac caccaccacc actaattgat taatacctag gtcgacgcga
     4321 tcctttcctg ggacccggca agaaccaaaa actcactctc ttcaaggaaa tccgtaatgt
     4381 taaacccgac acgatgaagc ttgtcgttgg atggaaagga aaagagttct acagggaaac
     4441 ttggacccgc ttcatggaag acagcttccc cattgttaac gaccaagaag tgatggatgt
     4501 tttccttgtt gtcaacatgc gtcccactag acccaaccgt tgttacaaat tcctggccca
     4561 acacgctctg cgttgcgacc ccgactatgt acctcatgac gtgattagga tcgtcgagcc
     4621 ttcatgggtg ggcagcaaca acgagtaccg catcagcctg gctaagaagg gcggcggctg
     4681 cccaataatg aaccttcact ctgagtacac caactcgttc gaacagttca tcgatcgtgt
     4741 catctgggag aacttctaca agcccatcgt ttacatcggt accgactctg ctgaagagga
     4801 ggaaattctc cttgaagttt ccctggtgtt caaagtaaag gagtttgcac cagacgcacc
     4861 tctgttcact ggtccggcgt attaaaacac gatacattgt tattagtaca tttattaagc
     4921 gctagattct gtgcgttgtt gatttacaga caattgttgt acgtatttta ataattcatt
     4981 aaatttataa tctttagggt ggtatgttag agcgaaaatc aaatgatttt cagcgtcttt
     5041 atatctgaat ttaaatatta aatcctcaat agatttgtaa aataggtttc gattagtttc
     5101 aaacaagggt tgtttttccg aaccgatggc tggactatct aatggatttt cgctcaacgc
     5161 cacaaaactt gccaaatctt gtagcagcaa tctagctttg tcgatattcg tttgtgtttt
     5221 gttttgtaat aaaggttcga cgtcgttcaa aatattatgc gcttttgtat ttctttcatc
     5281 actgtcgtta gtgtacaatt gactcgacgt aaacacgtta aataaagctt ggacatattt
     5341 aacatcgggc gtgttagctt tattaggccg attatcgtcg tcgtcccaac cctcgtcgtt
     5401 agaagttgct tccgaagacg attttgccat agccacacga cgcctattaa ttgtgtcggc
     5461 taacacgtcc gcgatcaaat ttgtagttga gctttttgga attatttctg attgcgggcg
     5521 tttttgggcg ggtttcaatc taactgtgcc cgattttaat tcagacaaca cgttagaaag
     5581 cgatggtgca ggcggtggta acatttcaga cggcaaatct actaatggcg gcggtggtgg
     5641 agctgatgat aaatctacca tcggtggagg cgcaggcggg gctggcggcg gaggcggagg
     5701 cggaggtggt ggcggtgatg cagacggcgg tttaggctca aatgtctctt taggcaacac
     5761 agtcggcacc tcaactattg tactggtttc gggcgccgtt tttggtttga ccggtctgag
     5821 acgagtgcga tttttttcgt ttctaatagc ttccaacaat tgttgtctgt cgtctaaagg
     5881 tgcagcgggt tgaggttccg tcggcattgg tggagcgggc ggcaattcag acatcgatgg
     5941 tggtggtggt ggtggaggcg ctggaatgtt aggcacggga gaaggtggtg gcggcggtgc
     6001 cgccggtata atttgttctg gtttagtttg ttcgcgcacg attgtgggca ccggcgcagg
     6061 cgccgctggc tgcacaacgg aaggtcgtct gcttcgaggc agcgcttggg gtggtggcaa
     6121 ttcaatatta taattggaat acaaatcgta aaaatctgct ataagcattg taatttcgct
     6181 atcgtttacc gtgccgatat ttaacaaccg ctcaatgtaa gcaattgtat tgtaaagaga
     6241 ttgtctcaag ctccgcacgc cgataacaag ccttttcatt tttactacag cattgtagtg
     6301 gcgagacact tcgctgtcgt cgacgtacat gtatgctttg ttgtcaaaaa cgtcgttggc
     6361 aagctttaaa atatttaaaa gaacatctct gttcagcacc actgtgttgt cgtaaatgtt
     6421 gtttttgata atttgcgctt ccgcagtatc gacacgttca aaaaattgat gcgcatcaat
     6481 tttgttgttc ctattattga ataaataaga ttgtacagat tcatatctac gattcgtcat
     6541 ggccaccaca aatgctacgc tgcaaacgct ggtacaattt tacgaaaact gcaaaaacgt
     6601 caaaactcgg tataaaataa tcaacgggcg ctttggcaaa atatctattt tatcgcacaa
     6661 gcccactagc aaattgtatt tgcagaaaac aatttcggcg cacaatttta acgctgacga
     6721 aataaaagtt caccagttaa tgagcgacca cccaaatttt ataaaaatct attttaatca
     6781 cggttccatc aacaaccaag tgatcgtgat ggactacatt gactgtcccg atttatttga
     6841 aacactacaa attaaaggcg agctttcgta ccaacttgtt agcaatatta ttagacagct
     6901 gtgtgaagcg ctcaacgatt tgcacaagca caatttcata cacaacgaca taaaactcga
     6961 aaatgtctta tatttcgaag cacttgatcg cgtgtatgtt tgcgattacg gattgtgcaa
     7021 acacgaaaac tcacttagcg tgcacgacgg cacgttggag tattttagtc cggaaaaaat
     7081 tcgacacaca actatgcacg tttcgtttga ctggtacgcg gcgtgttaac atacaagttg
     7141 ctaaccggcg gttcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     7201 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     7261 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     7321 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     7381 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     7441 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     7501 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     7561 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     7621 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     7681 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     7741 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     7801 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     7861 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     7921 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     7981 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
     8041 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     8101 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     8161 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     8221 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     8281 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     8341 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     8401 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     8461 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     8521 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     8581 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     8641 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     8701 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     8761 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     8821 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     8881 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     8941 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     9001 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     9061 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     9121 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     9181 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     9241 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     9301 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
     9361 aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga
     9421 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
     9481 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca
     9541 tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta
     9601 aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg
     9661 cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg
     9721 cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt
     9781 gcc
//
