LOCUS       V011697                10793 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V011697
VERSION     V011697
KEYWORDS    pAB-MBP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10793)
  AUTHORS   AB Vector
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 10793)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10793
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             76..579
                     /gene="PTP"
                     /label="Tyrosine-protein phosphatase"
                     /note="Tyrosine-protein phosphatase from Autographa
                     californica nuclear polyhedrosis virus. Accession#: P24656"
     CDS             2662..3291
                     /gene="LEF-2"
                     /label="Primase-associated factor LEF-2"
                     /note="Primase-associated factor LEF-2 from Autographa
                     californica nuclear polyhedrosis virus. Accession#: P41418"
     misc_recomb     3170..3997
                     /label="baculovirus recombination region (lef2/ORF603)"
                     /note="contains ORF603 and part of lef2"
     promoter        4001..4092
                     /label="polyhedrin promoter"
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             4152..5243
                     /label="MBP"
                     /note="maltose binding protein from E. coli"
     CDS             5262..5279
                     /label="thrombin site"
                     /note="thrombin recognition and cleavage site"
     misc_recomb     5808..7263
                     /label="baculovirus recombination region (ORF1629)"
                     /note="contains part of ORF1629"
     promoter        complement(8222..8252)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8267..8288)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(8576..9164)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(9338..10195)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(10196..10300)
                     /label="AmpR promoter"
     primer_bind     10774..10790
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
ORIGIN
        1 aagctttact cgtaaagcga gttgaaggat catatttagt tgcgtttatg agataagatt
       61 gaaagcacgt gtaaaatgtt tcccgcgcgt tggcacaact atttacaatg cggccaagtt
      121 ataaaagatt ctaatctgat atgttttaaa acacctttgc ggcccgagtt gtttgcgtac
      181 gtgactagcg aagaagatgt gtggaccgca gaacagatag taaaacaaaa ccctagtatt
      241 ggagcaataa tcgatttaac caacacgtct aaatattatg atggtgtgca ttttttgcgg
      301 gcgggcctgt tatacaaaaa aattcaagta cctggccaga ctttgccgcc tgaaagcata
      361 gttcaagaat ttattgacac ggtaaaagaa tttacagaaa agtgtcccgg catgttggtg
      421 ggcgtgcact gcacacacgg tattaatcgc accggttaca tggtgtgcag atatttaatg
      481 cacaccctgg gtattgcgcc gcaggaagcc atagatagat tcgaaaaagc cagaggtcac
      541 aaaattgaaa gacaaaatta cgttcaagat ttattaattt aattaatatt atttgcattc
      601 tttaacaaat actttatcct attttcaaat tgttgcgctt cttccagcga accaaaacta
      661 tgcttcgctt gctccgttta gcttgtagcc gatcagtggc gttgttccaa tcgacggtag
      721 gattaggccg gatattctcc accacaatgt tggcaacgtt gatgttacgt ttatgctttt
      781 ggttttccac gtacgtcttt tggccggtaa tagccgtaaa cgtagtgccg tcgcgcgtca
      841 cgcacaacac cggatgtttg cgcttgtccg cggggtattg aaccgcgcga tccgacaaat
      901 ccaccacttt ggcaactaaa tcggtgacct gcgcgtcttt tttctgcatt atttcgtctt
      961 tcttttgcat ggtttcctgg aagccggtgt acatgcggtt tagatcagtc atgacgcgcg
     1021 tgacctgcaa atctttggcc tcgatctgct tgtccttgat ggcaacgatg cgttcaataa
     1081 actcttgttt tttaacaagt tcctcggttt tttgcgccac caccgcttgc agcgcgtttg
     1141 tgtgctcggt gaatgtcgca atcagcttag tcaccaactg tttgctctcc tcctcccgtt
     1201 gtttgatcgc gggatcgtac ttgccggtgc agagcacttg aggaattact tcttctaaaa
     1261 gccattcttg taattctatg gcgtaaggca atttggactt cataatcagc tgaatcacgc
     1321 cggatttagt aatgagcact gtatgcggct gcaaatacag cgggtcgccc cttttcacga
     1381 cgctgttaga ggtagggccc ccattttgga tggtctgctc aaataacgat ttgtatttat
     1441 tgtctacatg aacacgtata gctttatcac aaactgtata ttttaaactg ttagcgacgt
     1501 ccttggccac gaaccggacc tgttggtcgc gctctagcac gtaccgcagg ttgaacgtat
     1561 cttctccaaa tttaaattct ccaattttaa cgcgagccat tttgatacac gtgtgtcgat
     1621 tttgcaacaa ctattgtttt ttaacgcaaa ctaaacttat tgtggtaagc aataattaaa
     1681 tatgggggaa catgcgccgc tacaacactc gtcgttatga acgcagacgg cgccggtctc
     1741 ggcgcaagcg gctaaaacgt gttgcgcgtt caacgcggca aacatcgcaa aagccaatag
     1801 tacagttttg atttgcatat taacggcgat tttttaaatt atcttattta ataaatagtt
     1861 atgacgccta caactccccg cccgcgttga ctcgctgcac ctcgagcagt tcgttgacgc
     1921 cttcctccgt gtggccgaac acgtcgagcg ggtggtcgat gaccagcggc gtgccgcacg
     1981 cgacgcacaa gtatctgtac accgaatgat cgtcgggcga aggcacgtcg gcctccaagt
     2041 ggcaatattg gcaaattcga aaatatatac agttgggttg tttgcgcata tctatcgtgg
     2101 cgttgggcat gtacgtccga acgttgattt gcatgcaagc cgaaattaaa tcattgcgat
     2161 tagtgcgatt aaaacgttgt acatcctcgc ttttaatcat gccgtcgatt aaatcgcgca
     2221 atcgagtcaa gtgatcaaag tgtggaataa tgttttcttt gtattcccga gtcaagcgca
     2281 gcgcgtattt taacaaacta gccatcttgt aagttagttt catttaatgc aactttatcc
     2341 aataatatat tatgtatcgc acgtcaagaa ttaacaatgc gcccgttgtc gcatctcaac
     2401 acgactatga tagagatcaa ataaagcgcg aattaaatag cttgcgacgc aacgtgcacg
     2461 atctgtgcac gcgttccggc acgagctttg attgtaataa gtttttacga agcgatgaca
     2521 tgacccccgt agtgacaacg atcacgccca aaagaactgc cgactacaaa attaccgagt
     2581 atgtcggtga cgttaaaact attaagccat ccaatcgacc gttagtcgaa tcaggaccgc
     2641 tggtgcgaga agccgcgaag tatggcgaat gcatcgtata acgtgtggag tccgctcatt
     2701 agagcgtcat gtttagacaa gaaagctaca tatttaattg atcccgatga ttttattgat
     2761 aaattgaccc taactccata cacggtattc tacaatggcg gggttttggt caaaatttcc
     2821 ggactgcgat tgtacatgct gttaacggct ccgcccacta ttaatgaaat taaaaattcc
     2881 aattttaaaa aacgcagcaa gagaaacatt tgtatgaaag aatgcgtaga aggaaagaaa
     2941 aatgtcgtcg acatgctgaa caacaagatt aatatgcctc cgtgtataaa aaaaatattg
     3001 aacgatttga aagaaaacaa tgtaccgcgc ggcggtatgt acaggaagag gtttatacta
     3061 aactgttaca ttgcaaacgt ggtttcgtgt gccaagtgtg aaaaccgatg tttaatcaag
     3121 gctctgacgc atttctacaa ccacgactcc aagtgtgtgg gtgaagtcat gcatctttta
     3181 atcaaatccc aagatgtgta taaaccacca aactgccaaa aaatgaaaac tgtcgacaag
     3241 ctctgtccgt ttgctggcaa ctgcaagggt ctcaatccta tttgtaatta ttgaataata
     3301 aaacaattat aaatgctaaa tttgtttttt attaacgata caaaccaaac gcaacaagaa
     3361 catttgtagt attatctata attgaaaacg cgtagttata atcgctgagg taatatttaa
     3421 aatcattttc aaatgattca cagttaattt gcgacaatat aattttattt tcacataaac
     3481 tagacgcctt gtcgtcttct tcttcgtatt ccttctcttt ttcatttttc tcctcataaa
     3541 aattaacata gttattatcg tatccatata tgtatctatc gtatagagta aattttttgt
     3601 tgtcataaat atatatgtct tttttaatgg ggtgtatagt accgctgcgc atagtttttc
     3661 tgtaatttac aacagtgcta ttttctggta gttcttcgga gtgtgttgct ttaattatta
     3721 aatttatata atcaatgaat ttgggatcgt cggttttgta caatatgttg ccggcatagt
     3781 acgcagcttc ttctagttca attacaccat tttttagcag caccggatta acataacttt
     3841 ccaaaatgtt gtacgaaccg ttaaacaaaa acagttcacc tcccttttct atactattgt
     3901 ctgcgagcag ttgtttgttg ttaaaaataa cagccattgt aatgagacgc acaaactaat
     3961 atcacaaact ggaaatgtct atcaatatat agttgctgat atcatggaga taattaaaat
     4021 gataaccatc tcgcaaataa ataagtattt tactgttttc gtaacagttt tgtaataaaa
     4081 aaacctataa atattccgga ttattcatac cgtcccacca tcgggcgcgg atcctagtag
     4141 aagaaatggc cgaagaaggt aaactggtaa tctggattaa cggcgataaa ggctataacg
     4201 gtctcgctga agtcggtaag aaattcgaga aagataccgg aattaaagtc accgttgagc
     4261 atccggataa actggaagag aaattcccac aggttgcggc aacaggcgat ggccctgaca
     4321 ttatcttctg ggcacacgac cgctttggtg gctacgctca atctggcctg ttggctgaaa
     4381 tcaccccgga caaagcgttc caggacaagc tgtatccgtt tacctgggat gccgtacgtt
     4441 acaacggcaa gctgattgct tacccgatcg ctgttgaagc gttatcgctg atttataaca
     4501 aagatctgct gccgaacccg ccaaaaacct gggaagagat cccggcgctg gataaagaac
     4561 tgaaagcgaa aggtaagagc gcgctgatgt tcaacctgca agaaccgtac ttcacctggc
     4621 cgctgattgc tgctgacggg ggttatgcgt tcaagtatga aaacggcaag tacgacatta
     4681 aagacgtggg cgtggataac gctggcgcga aagcgggtct gaccttcctg gttgacctga
     4741 ttaaaaacaa acacatgaat gcagacaccg attactccat cgcagaagct gcctttaata
     4801 aaggcgaaac agcgatgacc atcaacggcc cgtgggcatg gtccaacatc gacaccagca
     4861 aagtgaatta tggtgtaacg gtactgccga ccttcaaggg tcaaccatcc aaaccgttcg
     4921 ttggcgtgct gagcgcaggt attaacgccg ccagtccgaa caaagagctg gcaaaagagt
     4981 tcctcgaaaa ctatctgctg actgatgaag gtctggaagc ggttaataaa gacaaaccgc
     5041 tgggtgccgt agcgctgaag tcttacgagg aagagttggc gaaagatcca cgtattgccg
     5101 ccactatgga aaacgcccag aaaggtgaaa tcatgccgaa catcccgcag atgtccgctt
     5161 tctggtatgc cgtgcgtact gcggtgatca acgccgccag cggtcgtcag actgtcgatg
     5221 aagccctgaa agacgcgcag actaattcga gctctgcagg cctagttcct cgtggatctc
     5281 tagatggcgg aattcatggc tcccgggcag agcggccgct gcagatctga tcctttcctg
     5341 ggacccggca agaaccaaaa actcactctc ttcaaggaaa tccgtaatgt taaacccgac
     5401 acgatgaagc ttgtcgttgg atggaaagga aaagagttct acagggaaac ttggacccgc
     5461 ttcatggaag acagcttccc cattgttaac gaccaagaag tgatggatgt tttccttgtt
     5521 gtcaacatgc gtcccactag acccaaccgt tgttacaaat tcctggccca acacgctctg
     5581 cgttgcgacc ccgactatgt acctcatgac gtgattagga tcgtcgagcc ttcatgggtg
     5641 ggcagcaaca acgagtaccg catcagcctg gctaagaagg gcggcggctg cccaataatg
     5701 aaccttcact ctgagtacac caactcgttc gaacagttca tcgatcgtgt catctgggag
     5761 aacttctaca agcccatcgt ttacatcggt accgactctg ctgaagagga ggaaattctc
     5821 cttgaagttt ccctggtgtt caaagtaaag gagtttgcac cagacgcacc tctgttcact
     5881 ggtccggcgt attaaaacac gatacattgt tattagtaca tttattaagc gctagattct
     5941 gtgcgttgtt gatttacaga caattgttgt acgtatttta ataattcatt aaatttataa
     6001 tctttagggt ggtatgttag agcgaaaatc aaatgatttt cagcgtcttt atatctgaat
     6061 ttaaatatta aatcctcaat agatttgtaa aataggtttc gattagtttc aaacaagggt
     6121 tgtttttccg aaccgatggc tggactatct aatggatttt cgctcaacgc cacaaaactt
     6181 gccaaatctt gtagcagcaa tctagctttg tcgatattcg tttgtgtttt gttttgtaat
     6241 aaaggttcga cgtcgttcaa aatattatgc gcttttgtat ttctttcatc actgtcgtta
     6301 gtgtacaatt gactcgacgt aaacacgtta aataaagctt ggacatattt aacatcgggc
     6361 gtgttagctt tattaggccg attatcgtcg tcgtcccaac cctcgtcgtt agaagttgct
     6421 tccgaagacg attttgccat agccacacga cgcctattaa ttgtgtcggc taacacgtcc
     6481 gcgatcaaat ttgtagttga gctttttgga attatttctg attgcgggcg tttttgggcg
     6541 ggtttcaatc taactgtgcc cgattttaat tcagacaaca cgttagaaag cgatggtgca
     6601 ggcggtggta acatttcaga cggcaaatct actaatggcg gcggtggtgg agctgatgat
     6661 aaatctacca tcggtggagg cgcaggcggg gctggcggcg gaggcggagg cggaggtggt
     6721 ggcggtgatg cagacggcgg tttaggctca aatgtctctt taggcaacac agtcggcacc
     6781 tcaactattg tactggtttc gggcgccgtt tttggtttga ccggtctgag acgagtgcga
     6841 tttttttcgt ttctaatagc ttccaacaat tgttgtctgt cgtctaaagg tgcagcgggt
     6901 tgaggttccg tcggcattgg tggagcgggc ggcaattcag acatcgatgg tggtggtggt
     6961 ggtggaggcg ctggaatgtt aggcacggga gaaggtggtg gcggcggtgc cgccggtata
     7021 atttgttctg gtttagtttg ttcgcgcacg attgtgggca ccggcgcagg cgccgctggc
     7081 tgcacaacgg aaggtcgtct gcttcgaggc agcgcttggg gtggtggcaa ttcaatatta
     7141 taattggaat acaaatcgta aaaatctgct ataagcattg taatttcgct atcgtttacc
     7201 gtgccgatat ttaacaaccg ctcaatgtaa gcaattgtat tgtaaagaga ttgtctcaag
     7261 ctccgcacgc cgataacaag ccttttcatt tttactacag cattgtagtg gcgagacact
     7321 tcgctgtcgt cgacgtacat gtatgctttg ttgtcaaaaa cgtcgttggc aagctttaaa
     7381 atatttaaaa gaacatctct gttcagcacc actgtgttgt cgtaaatgtt gtttttgata
     7441 atttgcgctt ccgcagtatc gacacgttca aaaaattgat gcgcatcaat tttgttgttc
     7501 ctattattga ataaataaga ttgtacagat tcatatctac gattcgtcat ggccaccaca
     7561 aatgctacgc tgcaaacgct ggtacaattt tacgaaaact gcaaaaacgt caaaactcgg
     7621 tataaaataa tcaacgggcg ctttggcaaa atatctattt tatcgcacaa gcccactagc
     7681 aaattgtatt tgcagaaaac aatttcggcg cacaatttta acgctgacga aataaaagtt
     7741 caccagttaa tgagcgacca cccaaatttt ataaaaatct attttaatca cggttccatc
     7801 aacaaccaag tgatcgtgat ggactacatt gactgtcccg atttatttga aacactacaa
     7861 attaaaggcg agctttcgta ccaacttgtt agcaatatta ttagacagct gtgtgaagcg
     7921 ctcaacgatt tgcacaagca caatttcata cacaacgaca taaaactcga aaatgtctta
     7981 tatttcgaag cacttgatcg cgtgtatgtt tgcgattacg gattgtgcaa acacgaaaac
     8041 tcacttagcg tgcacgacgg cacgttggag tattttagtc cggaaaaaat tcgacacaca
     8101 actatgcacg tttcgtttga ctggtacgcg gcgtgttaac atacaagttg ctaaccggcg
     8161 gttcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     8221 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     8281 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     8341 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     8401 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     8461 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     8521 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     8581 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     8641 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     8701 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     8761 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     8821 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     8881 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     8941 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     9001 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     9061 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     9121 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     9181 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     9241 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     9301 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     9361 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     9421 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     9481 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     9541 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     9601 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     9661 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     9721 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     9781 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     9841 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     9901 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     9961 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
    10021 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
    10081 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
    10141 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
    10201 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
    10261 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
    10321 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
    10381 cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
    10441 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
    10501 gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga
    10561 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
    10621 accgcatcag gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
    10681 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
    10741 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gcc
//
