LOCUS       V011702                 9698 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V011702
VERSION     V011702
KEYWORDS    pAB-6xHis
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9698)
  AUTHORS   AB Vector
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 9698)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9698
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             76..579
                     /gene="PTP"
                     /label="Tyrosine-protein phosphatase"
                     /note="Tyrosine-protein phosphatase from Autographa
                     californica nuclear polyhedrosis virus. Accession#: P24656"
     CDS             2662..3291
                     /gene="LEF-2"
                     /label="Primase-associated factor LEF-2"
                     /note="Primase-associated factor LEF-2 from Autographa
                     californica nuclear polyhedrosis virus. Accession#: P41418"
     misc_recomb     3170..3997
                     /label="baculovirus recombination region (lef2/ORF603)"
                     /note="contains ORF603 and part of lef2"
     promoter        4001..4092
                     /label="polyhedrin promoter"
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             4138..4140
                     /codon_start=1
                     /product="start codon"
                     /label="start codon"
                     /note="ATG"
                     /translation="M"
     CDS             4147..4164
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             4174..4191
                     /label="thrombin site"
                     /note="thrombin recognition and cleavage site"
     misc_feature    4192..4251
                     /label="MCS"
                     /note="MCS"
                     /note="multiple cloning site"
     misc_recomb     4713..6168
                     /label="baculovirus recombination region (ORF1629)"
                     /note="contains part of ORF1629"
     primer_bind     complement(7079..7095)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(7103..7119)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7127..7157)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7172..7193)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7481..8069)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8243..9100)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(9101..9205)
                     /label="AmpR promoter"
     primer_bind     9679..9695
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
ORIGIN
        1 aagctttact cgtaaagcga gttgaaggat catatttagt tgcgtttatg agataagatt
       61 gaaagcacgt gtaaaatgtt tcccgcgcgt tggcacaact atttacaatg cggccaagtt
      121 ataaaagatt ctaatctgat atgttttaaa acacctttgc ggcccgagtt gtttgcgtac
      181 gtgactagcg aagaagatgt gtggaccgca gaacagatag taaaacaaaa ccctagtatt
      241 ggagcaataa tcgatttaac caacacgtct aaatattatg atggtgtgca ttttttgcgg
      301 gcgggcctgt tatacaaaaa aattcaagta cctggccaga ctttgccgcc tgaaagcata
      361 gttcaagaat ttattgacac ggtaaaagaa tttacagaaa agtgtcccgg catgttggtg
      421 ggcgtgcact gcacacacgg tattaatcgc accggttaca tggtgtgcag atatttaatg
      481 cacaccctgg gtattgcgcc gcaggaagcc atagatagat tcgaaaaagc cagaggtcac
      541 aaaattgaaa gacaaaatta cgttcaagat ttattaattt aattaatatt atttgcattc
      601 tttaacaaat actttatcct attttcaaat tgttgcgctt cttccagcga accaaaacta
      661 tgcttcgctt gctccgttta gcttgtagcc gatcagtggc gttgttccaa tcgacggtag
      721 gattaggccg gatattctcc accacaatgt tggcaacgtt gatgttacgt ttatgctttt
      781 ggttttccac gtacgtcttt tggccggtaa tagccgtaaa cgtagtgccg tcgcgcgtca
      841 cgcacaacac cggatgtttg cgcttgtccg cggggtattg aaccgcgcga tccgacaaat
      901 ccaccacttt ggcaactaaa tcggtgacct gcgcgtcttt tttctgcatt atttcgtctt
      961 tcttttgcat ggtttcctgg aagccggtgt acatgcggtt tagatcagtc atgacgcgcg
     1021 tgacctgcaa atctttggcc tcgatctgct tgtccttgat ggcaacgatg cgttcaataa
     1081 actcttgttt tttaacaagt tcctcggttt tttgcgccac caccgcttgc agcgcgtttg
     1141 tgtgctcggt gaatgtcgca atcagcttag tcaccaactg tttgctctcc tcctcccgtt
     1201 gtttgatcgc gggatcgtac ttgccggtgc agagcacttg aggaattact tcttctaaaa
     1261 gccattcttg taattctatg gcgtaaggca atttggactt cataatcagc tgaatcacgc
     1321 cggatttagt aatgagcact gtatgcggct gcaaatacag cgggtcgccc cttttcacga
     1381 cgctgttaga ggtagggccc ccattttgga tggtctgctc aaataacgat ttgtatttat
     1441 tgtctacatg aacacgtata gctttatcac aaactgtata ttttaaactg ttagcgacgt
     1501 ccttggccac gaaccggacc tgttggtcgc gctctagcac gtaccgcagg ttgaacgtat
     1561 cttctccaaa tttaaattct ccaattttaa cgcgagccat tttgatacac gtgtgtcgat
     1621 tttgcaacaa ctattgtttt ttaacgcaaa ctaaacttat tgtggtaagc aataattaaa
     1681 tatgggggaa catgcgccgc tacaacactc gtcgttatga acgcagacgg cgccggtctc
     1741 ggcgcaagcg gctaaaacgt gttgcgcgtt caacgcggca aacatcgcaa aagccaatag
     1801 tacagttttg atttgcatat taacggcgat tttttaaatt atcttattta ataaatagtt
     1861 atgacgccta caactccccg cccgcgttga ctcgctgcac ctcgagcagt tcgttgacgc
     1921 cttcctccgt gtggccgaac acgtcgagcg ggtggtcgat gaccagcggc gtgccgcacg
     1981 cgacgcacaa gtatctgtac accgaatgat cgtcgggcga aggcacgtcg gcctccaagt
     2041 ggcaatattg gcaaattcga aaatatatac agttgggttg tttgcgcata tctatcgtgg
     2101 cgttgggcat gtacgtccga acgttgattt gcatgcaagc cgaaattaaa tcattgcgat
     2161 tagtgcgatt aaaacgttgt acatcctcgc ttttaatcat gccgtcgatt aaatcgcgca
     2221 atcgagtcaa gtgatcaaag tgtggaataa tgttttcttt gtattcccga gtcaagcgca
     2281 gcgcgtattt taacaaacta gccatcttgt aagttagttt catttaatgc aactttatcc
     2341 aataatatat tatgtatcgc acgtcaagaa ttaacaatgc gcccgttgtc gcatctcaac
     2401 acgactatga tagagatcaa ataaagcgcg aattaaatag cttgcgacgc aacgtgcacg
     2461 atctgtgcac gcgttccggc acgagctttg attgtaataa gtttttacga agcgatgaca
     2521 tgacccccgt agtgacaacg atcacgccca aaagaactgc cgactacaaa attaccgagt
     2581 atgtcggtga cgttaaaact attaagccat ccaatcgacc gttagtcgaa tcaggaccgc
     2641 tggtgcgaga agccgcgaag tatggcgaat gcatcgtata acgtgtggag tccgctcatt
     2701 agagcgtcat gtttagacaa gaaagctaca tatttaattg atcccgatga ttttattgat
     2761 aaattgaccc taactccata cacggtattc tacaatggcg gggttttggt caaaatttcc
     2821 ggactgcgat tgtacatgct gttaacggct ccgcccacta ttaatgaaat taaaaattcc
     2881 aattttaaaa aacgcagcaa gagaaacatt tgtatgaaag aatgcgtaga aggaaagaaa
     2941 aatgtcgtcg acatgctgaa caacaagatt aatatgcctc cgtgtataaa aaaaatattg
     3001 aacgatttga aagaaaacaa tgtaccgcgc ggcggtatgt acaggaagag gtttatacta
     3061 aactgttaca ttgcaaacgt ggtttcgtgt gccaagtgtg aaaaccgatg tttaatcaag
     3121 gctctgacgc atttctacaa ccacgactcc aagtgtgtgg gtgaagtcat gcatctttta
     3181 atcaaatccc aagatgtgta taaaccacca aactgccaaa aaatgaaaac tgtcgacaag
     3241 ctctgtccgt ttgctggcaa ctgcaagggt ctcaatccta tttgtaatta ttgaataata
     3301 aaacaattat aaatgctaaa tttgtttttt attaacgata caaaccaaac gcaacaagaa
     3361 catttgtagt attatctata attgaaaacg cgtagttata atcgctgagg taatatttaa
     3421 aatcattttc aaatgattca cagttaattt gcgacaatat aattttattt tcacataaac
     3481 tagacgcctt gtcgtcttct tcttcgtatt ccttctcttt ttcatttttc tcctcataaa
     3541 aattaacata gttattatcg tatccatata tgtatctatc gtatagagta aattttttgt
     3601 tgtcataaat atatatgtct tttttaatgg ggtgtatagt accgctgcgc atagtttttc
     3661 tgtaatttac aacagtgcta ttttctggta gttcttcgga gtgtgttgct ttaattatta
     3721 aatttatata atcaatgaat ttgggatcgt cggttttgta caatatgttg ccggcatagt
     3781 acgcagcttc ttctagttca attacaccat tttttagcag caccggatta acataacttt
     3841 ccaaaatgtt gtacgaaccg ttaaacaaaa acagttcacc tcccttttct atactattgt
     3901 ctgcgagcag ttgtttgttg ttaaaaataa cagccattgt aatgagacgc acaaactaat
     3961 atcacaaact ggaaatgtct atcaatatat agttgctgat atcatggaga taattaaaat
     4021 gataaccatc tcgcaaataa ataagtattt tactgttttc gtaacagttt tgtaataaaa
     4081 aaacctataa atattccgga ttattcatac cgtcccacca tcgggcgcgg atccaaaatg
     4141 agcggtcacc atcaccacca tcacggtgat caactagttc ctcgtggatc acccgggtac
     4201 cttctagaat tccggagcgg ccgctgcaga tctgatcctt tcctgggacc cggcaagaac
     4261 caaaaactca ctctcttcaa ggaaatccgt aatgttaaac ccgacacgat gaagcttgtc
     4321 gttggatgga aaggaaaaga gttctacagg gaaacttgga cccgcttcat ggaagacagc
     4381 ttccccattg ttaacgacca agaagtgatg gatgttttcc ttgttgtcaa catgcgtccc
     4441 actagaccca accgttgtta caaattcctg gcccaacacg ctctgcgttg cgaccccgac
     4501 tatgtacctc atgacgtgat taggatcgtc gagccttcat gggtgggcag caacaacgag
     4561 taccgcatca gcctggctaa gaagggcggc ggctgcccaa taatgaacct tcactctgag
     4621 tacaccaact cgttcgaaca gttcatcgat cgtgtcatct gggagaactt ctacaagccc
     4681 atcgtttaca tcggtaccga ctctgctgaa gaggaggaaa ttctccttga agtttccctg
     4741 gtgttcaaag taaaggagtt tgcaccagac gcacctctgt tcactggtcc ggcgtattaa
     4801 aacacgatac attgttatta gtacatttat taagcgctag attctgtgcg ttgttgattt
     4861 acagacaatt gttgtacgta ttttaataat tcattaaatt tataatcttt agggtggtat
     4921 gttagagcga aaatcaaatg attttcagcg tctttatatc tgaatttaaa tattaaatcc
     4981 tcaatagatt tgtaaaatag gtttcgatta gtttcaaaca agggttgttt ttccgaaccg
     5041 atggctggac tatctaatgg attttcgctc aacgccacaa aacttgccaa atcttgtagc
     5101 agcaatctag ctttgtcgat attcgtttgt gttttgtttt gtaataaagg ttcgacgtcg
     5161 ttcaaaatat tatgcgcttt tgtatttctt tcatcactgt cgttagtgta caattgactc
     5221 gacgtaaaca cgttaaataa agcttggaca tatttaacat cgggcgtgtt agctttatta
     5281 ggccgattat cgtcgtcgtc ccaaccctcg tcgttagaag ttgcttccga agacgatttt
     5341 gccatagcca cacgacgcct attaattgtg tcggctaaca cgtccgcgat caaatttgta
     5401 gttgagcttt ttggaattat ttctgattgc gggcgttttt gggcgggttt caatctaact
     5461 gtgcccgatt ttaattcaga caacacgtta gaaagcgatg gtgcaggcgg tggtaacatt
     5521 tcagacggca aatctactaa tggcggcggt ggtggagctg atgataaatc taccatcggt
     5581 ggaggcgcag gcggggctgg cggcggaggc ggaggcggag gtggtggcgg tgatgcagac
     5641 ggcggtttag gctcaaatgt ctctttaggc aacacagtcg gcacctcaac tattgtactg
     5701 gtttcgggcg ccgtttttgg tttgaccggt ctgagacgag tgcgattttt ttcgtttcta
     5761 atagcttcca acaattgttg tctgtcgtct aaaggtgcag cgggttgagg ttccgtcggc
     5821 attggtggag cgggcggcaa ttcagacatc gatggtggtg gtggtggtgg aggcgctgga
     5881 atgttaggca cgggagaagg tggtggcggc ggtgccgccg gtataatttg ttctggttta
     5941 gtttgttcgc gcacgattgt gggcaccggc gcaggcgccg ctggctgcac aacggaaggt
     6001 cgtctgcttc gaggcagcgc ttggggtggt ggcaattcaa tattataatt ggaatacaaa
     6061 tcgtaaaaat ctgctataag cattgtaatt tcgctatcgt ttaccgtgcc gatatttaac
     6121 aaccgctcaa tgtaagcaat tgtattgtaa agagattgtc tcaagctccg cacgccgata
     6181 acaagccttt tcatttttac tacagcattg tagtggcgag acacttcgct gtcgtcgacg
     6241 tacatgtatg ctttgttgtc aaaaacgtcg ttggcaagct ttaaaatatt taaaagaaca
     6301 tctctgttca gcaccactgt gttgtcgtaa atgttgtttt tgataatttg cgcttccgca
     6361 gtatcgacac gttcaaaaaa ttgatgcgca tcaattttgt tgttcctatt attgaataaa
     6421 taagattgta cagattcata tctacgattc gtcatggcca ccacaaatgc tacgctgcaa
     6481 acgctggtac aattttacga aaactgcaaa aacgtcaaaa ctcggtataa aataatcaac
     6541 gggcgctttg gcaaaatatc tattttatcg cacaagccca ctagcaaatt gtatttgcag
     6601 aaaacaattt cggcgcacaa ttttaacgct gacgaaataa aagttcacca gttaatgagc
     6661 gaccacccaa attttataaa aatctatttt aatcacggtt ccatcaacaa ccaagtgatc
     6721 gtgatggact acattgactg tcccgattta tttgaaacac tacaaattaa aggcgagctt
     6781 tcgtaccaac ttgttagcaa tattattaga cagctgtgtg aagcgctcaa cgatttgcac
     6841 aagcacaatt tcatacacaa cgacataaaa ctcgaaaatg tcttatattt cgaagcactt
     6901 gatcgcgtgt atgtttgcga ttacggattg tgcaaacacg aaaactcact tagcgtgcac
     6961 gacggcacgt tggagtattt tagtccggaa aaaattcgac acacaactat gcacgtttcg
     7021 tttgactggt acgcggcgtg ttaacataca agttgctaac cggcggttcg taatcatggt
     7081 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     7141 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     7201 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     7261 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     7321 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     7381 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     7441 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     7501 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     7561 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     7621 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     7681 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     7741 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     7801 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     7861 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     7921 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     7981 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     8041 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     8101 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     8161 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     8221 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     8281 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     8341 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     8401 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     8461 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     8521 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     8581 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     8641 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     8701 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     8761 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     8821 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     8881 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     8941 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     9001 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     9061 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     9121 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     9181 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag
     9241 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
     9301 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     9361 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     9421 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     9481 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
     9541 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     9601 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     9661 tttcccagtc acgacgttgt aaaacgacgg ccagtgcc
//
