LOCUS       V009657                 7954 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009657
VERSION     V009657
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7954)
  AUTHORS   Adachi K, Enoki T, Kawano Y, Veraz M, Nakai H.
  TITLE     Drawing a high-resolution functional map of adeno-associated virus
            capsid by massively parallel sequencing
  JOURNAL   Nat Commun 5, 3075 (2014)
   PUBMED   24435020
REFERENCE   2  (bases 1 to 7954)
  AUTHORS   Nakai H.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2013) Department of Molecular and Medical
            Genetics, Oregon Health and Science University, 3181 SW Sam Jackson
            Park Road, Portland, OR 97239, USA
REFERENCE   3  (bases 1 to 7954)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7954)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Nat Commun
            5, 3075 (2014)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (07-MAY-2013) Department of Molecular and Medical Genetics, Oregon
            Health and Science University, 3181 SW Sam Jackson Park Road,
            Portland, OR 97239, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7954
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   6..146
                     /label="AAV2 ITR"
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
     CDS             322..2184
                     /gene="Rep78"
                     /label="Protein Rep78"
                     /note="Protein Rep78 from Adeno-associated virus 2 (isolate
                     Srivastava/1982). Accession#: Q89268"
     CDS             4256..4267
                     /label="WELQut site"
                     /note="WELQut protease recognition and cleavage site"
     gap             4471..4482
                     /estimated_length=12
     gap             4503..4514
                     /estimated_length=12
     repeat_region   4670..4810
                     /label="AAV2 ITR"
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
     primer_bind     complement(4849..4865)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      5078..5533
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5815..5919
                     /label="AmpR promoter"
     CDS             5920..6777
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      6951..7539
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    7827..7848
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        7863..7893
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    7901..7917
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7925..7941
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
ORIGIN
        1 aagctcctgc aggcagctgc gcgctcgctc gctcactgag gccgcccggg caaagcccgg
       61 gcgtcgggcg acctttggtc gcccggcctc agtgagcgag cgagcgcgca gagagggagt
      121 ggccaactcc atcactaggg gttcctggag gggtggagtc gtgacgtgaa ttacgtcata
      181 gggttaggga ggtcctgtat tagaggtcac gtgagtgttt tgcgacattt tgcgacacca
      241 tgtggtcacg ctgggtattt aagcccgagt gagcacgcag ggtctccatt ttgaagcggg
      301 aggtttgaac gcgcagccgc catgccgggg ttttacgaga ttgtgattaa ggtccccagc
      361 gaccttgacg ggcatctgcc cggcatttct gacagctttg tgaactgggt ggccgagaag
      421 gaatgggagt tgccgccaga ttctgacatg gatctgaatc tgattgagca ggcacccctg
      481 accgtggccg agaagctgca gcgcgacttt ctgacggaat ggcgccgtgt gagtaaggcc
      541 ccggaggccc ttttctttgt gcaatttgag aagggagaga gctacttcca catgcacgtg
      601 ctcgtggaaa ccaccggggt gaaatccatg gttttgggac gtttcctgag tcagattcgc
      661 gaaaaactga ttcagagaat ttaccgcggg atcgagccga ctttgccaaa ctggttcgcg
      721 gtcacaaaga ccagaaatgg cgccggaggc gggaacaagg tggtggatga gtgctacatc
      781 cccaattact tgctccccaa aacccagcct gagctccagt gggcgtggac taatatggaa
      841 cagtatttaa gcgcctgttt gaatctcacg gagcgtaaac ggttggtggc gcagcatctg
      901 acgcacgtgt cgcagacgca ggagcagaac aaagagaatc agaatcccaa ttctgatgcg
      961 ccggtgatca gatcaaaaac ttcagccagg tacatggagc tggtcgggtg gctcgtggac
     1021 aaggggatta cctcggagaa gcagtggatc caggaggacc aggcctcata catctccttc
     1081 aatgcggcct ccaactcgcg gtcccaaatc aaggctgcct tggacaatgc gggaaagatt
     1141 atgagcctga ctaaaaccgc ccccgactac ctggtgggcc agcagcccgt ggaggacatt
     1201 tccagcaatc ggatttataa aattttggaa ctaaacgggt acgatcccca atatgcggct
     1261 tccgtctttc tgggatgggc cacgaaaaag ttcggcaaga ggaacaccat ctggctgttt
     1321 gggcctgcaa ctaccgggaa gaccaacatc gcggaggcca tagcccacac tgtgcccttc
     1381 tacgggtgcg taaactggac caatgagaac tttcccttca acgactgtgt cgacaagatg
     1441 gtgatctggt gggaggaggg gaagatgacc gccaaggtcg tggagtcggc caaagccatt
     1501 ctcggaggaa gcaaggtgcg cgtggaccag aaatgcaagt cctcggccca gatagacccg
     1561 actcccgtga tcgtcacctc caacaccaac atgtgcgccg tgattgacgg gaactcaacg
     1621 accttcgaac accagcagcc gttgcaagac cggatgttca aatttgaact cacccgccgt
     1681 ctggatcatg actttgggaa ggtcaccaag caggaagtca aagacttttt ccggtgggca
     1741 aaggatcacg tggttgaggt ggagcatgaa ttctacgtca aaaagggtgg agccaagaaa
     1801 agacccgccc ccagtgacgc agatataagt gagcccaaac gggtgcgcga gtcagttgcg
     1861 cagccatcga cgtcagacgc ggaagcttcg atcaactacg cggacaggta ccaaaacaaa
     1921 tgttctcgtc acgtgggcat gaatctgatg ctgtttccct gcagacaatg cgagagactg
     1981 aatcagaatt caaatatctg cttcactcac ggtgtcaaag actgtttaga gtgctttccc
     2041 gtgtcagaat ctcaacccgt ttctgtcgtc aaaaaggcgt atcagaaact gtgctacatt
     2101 catcacatca tgggaaaggt gccagacgct tgcactgcat gcgacctggt caatgtggac
     2161 ttggatgact gtgtttctga acaataaatg acttaaacca ggtatggctg ccgatggtta
     2221 tcttccagat tggctcgagg acaaccttag tgaaggaatt cgcgagtggt gggctttgaa
     2281 acctggagcc cctcaaccca aggcaaatca acaacatcaa gacaacgctc gaggtcttgt
     2341 gcttccgggt tacaaatacc ttggacccgg caacggactc gacaaggggg agccggtcaa
     2401 cgcagcagac gcggcggccc tcgagcacga caaggcctac gaccagcagc tcaaggccgg
     2461 agacaacccg tacctcaagt acaaccacgc cgacgccgag ttccaggagc ggctcaaaga
     2521 agatacgtct tttgggggca acctcgggcg agcagtcttc caggccaaaa agaggcttct
     2581 tgaacctctt ggtctggttg aggaagcggc taagacggct cctggaaaga agaggcctgt
     2641 agagcagtct cctcaggaac cggactcctc cgcgggtatt ggcaaatcgg gtgcacagcc
     2701 cgctaaaaag agactcaatt tcggtcagac tggcgacaca gagtcagttc cggaccctca
     2761 accaatcgga gaacctcccg cagccccctc aggtgtggga tctcttacaa tggcttcagg
     2821 tggtggcgca ccagtggcag acaataacga aggtgccgat ggagtgggta gttcctcggg
     2881 aaattggcat tgcgattccc aatggctggg ggacagagtc atcaccacca gcacccgaac
     2941 ctgggccctg cccacctaca acaatcacct ctacaagcaa atctccaaca gcacatctgg
     3001 aggatcttca aatgacaacg cctacttcgg ctacagcacc ccctgggggt attttgactt
     3061 caacagattc cactgccact tctcaccacg tgactggcag cgactcatca acaacaactg
     3121 gggattccgg cctaagcgac tcaacttcaa gctcttcaac attcaggtca aagaggttac
     3181 ggacaacaat ggagtcaaga ccatcgccaa taaccttacc agcacggtcc aggtcttcac
     3241 ggactcagac tatcagctcc cgtacgtgct cgggtcggct cacgagggct gcctcccgcc
     3301 gttcccagcg gacgttttca tgattcctca gtacgggtat ctgacgctta atgatggaag
     3361 ccaggccgtg ggtcgttcgt ccttttactg cctggaatat ttcccgtcgc aaatgctaag
     3421 aacgggtaac aacttccagt tcagctacga gtttgagaac gtacctttcc atagcagcta
     3481 cgctcacagc caaagcctgg accgactaat gaatccactc atcgaccaat acttgtacta
     3541 tctctcaaag actattaacg gttctggaca gaatcaacaa acgctaaaat tcagtgtggc
     3601 cggacccagc aacatggctg tccagggaag aaactacata cctggaccca gctaccgaca
     3661 acaacgtgtc tcaaccactg tgactcaaaa caacaacagc gaatttgctt ggcctggagc
     3721 ttcttcttgg gctctcaatg gacgtaatag cttgatgaat cctggacctg ctatggccag
     3781 ccacaaagaa ggagaggacc gtttctttcc cttaagtgga tctttaattt ttggcaaaca
     3841 aggaactgga agagacaacg tggatgcgga caaagtcatg ataaccaacg aagaagaaat
     3901 taaaactact aacccggtag caacggagtc ctatggacaa gtggccacaa accaccagag
     3961 tgcccaagca caggcgcaga ccggctgggt tcaaaaccaa ggaatacttc cgggtatggt
     4021 ttggcaggac agagatgtgt acctgcaagg acccatatgg gccaaaattc ctcacacgga
     4081 cggcaacttt cacccttctc cgctgatggg agggtttgga atgaagcacc cgcctcctca
     4141 gatcctcatc aaaaacacac ctgtacctgc ggatcctcca acggccttca acaaggacaa
     4201 gctgaactct ttcatcaccc agtattctac tggccaagtc agcgtggaga tcgagtggga
     4261 gctgcagaag gaaaacagca agcgctggaa cccggagatc cagtacactt ccaactatta
     4321 caagtctaat aatgttgaat ttgctgttaa tactgaaggt gtatatagtg aaccccgccc
     4381 cattggcacc agatacctga ctcgtaatct gtaattgctt gttaatcaat aaaccgttta
     4441 attcacctac gtacttccgc tcatgctagc nnnnnnnnnn nnacggaaat acgatgtcgg
     4501 gannnnnnnn nnnntgtaca gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     4561 ggagttcgtg accgccgcca ccggttgatt cgtttcagtt gaactttggt ctctgcgaag
     4621 ggcgaattcg ttttagataa gtagcatggc gggttaatca ttaactacaa ggaaccccta
     4681 gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca
     4741 aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc
     4801 tgcctgcagg tcgactctag aggatccccg ggtaccgagc tcgaattcac tggccgtcgt
     4861 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
     4921 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     4981 gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat tttctcctta cgcatctgtg
     5041 cggtatttca caccgcatac gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta
     5101 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     5161 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     5221 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     5281 aaaaaacttg atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     5341 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     5401 acactcaacc ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggcc
     5461 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     5521 acgtttacaa ttttatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     5581 cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
     5641 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
     5701 tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     5761 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     5821 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     5881 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     5941 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     6001 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     6061 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     6121 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     6181 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     6241 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     6301 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     6361 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     6421 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     6481 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     6541 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     6601 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     6661 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     6721 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     6781 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     6841 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     6901 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     6961 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     7021 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     7081 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
     7141 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     7201 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     7261 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     7321 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     7381 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     7441 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     7501 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     7561 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     7621 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     7681 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     7741 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     7801 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     7861 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
     7921 cacacaggaa acagctatga ccatgattac gccg
//
