LOCUS       40924_3271        6990 bp DNA     circular SYN 17-DEC-2018
DEFINITION  SiRNA vector pAAV9(5)-hSyn-DsRed2N1-EGFP-siRNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6990)
  AUTHORS   Michel U, Malik I, Ebert S, Bahr M, Kugler S.
  TITLE     Long-term in vivo and in vitro AAV-2-mediated RNA interference in 
            rat retinal ganglion cells and cultured primary neurons
  JOURNAL   Biochem. Biophys. Res. Commun. 326 (2), 307-312 (2005)
  PUBMED    15582578
REFERENCE   2  (bases 1 to 6990)
  AUTHORS   Michel U, Malik I, Kuegler S.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUN-2004) Neurology, University of Goettingen, Waldweg
            33, Goettingen 37073, Germany
REFERENCE   3  (bases 1 to 6990)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6990)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem. 
            Biophys. Res. Commun."; date: "2005"; volume: "326"; issue: "2"; 
            pages: "307-312"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (01-JUN-2004) Neurology, University of Goettingen, Waldweg 33, 
            Goettingen 37073, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6990
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    complement(51..185)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     intron          complement(201..334)
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     CDS             complement(351..1025)
                     /label=DsRed2
                     /note="improved tetrameric variant of DsRed fluorescent
                     protein"
     misc_feature    complement(1044..1062)
                     /note="MCS; multiple cloning site"
     promoter        complement(1068..1515)
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     promoter        1543..1757
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     misc_feature    1759..1822
                     /note="siEGFP; 64 bp siRNA sequence"
     gene            complement(1827..4090)
                     /gene="9(5)"
                     /label=9(5)
                     /note="partial sequence from the non-coding porcine RNA UM
                     9(5)"
     repeat_region   4110..4250
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4325..4780
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5062..5166
                     /label=AmpR promoter
     CDS             5167..6024
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      6198..6786
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     repeat_region   6848..6988
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
ORIGIN
        1 ggccgcacgc gatcgaattc ctgcagcccg ggggatcttc gatgctagac gatccagaca
       61 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
      121 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
      181 aagttgctcg aagtcgacac ctgtggagag aaaggcaaag tggatgtcag taagaccaat
      241 aggtgcctat cagaaacgca agagtcttct ctgtctcgac aagcccagtt tctatgtggt
      301 ctccttaaac ctgtcttgta accttgatac ttacctgcgg ccggccgcta caggaacagg
      361 tggtggcggc cctcggtgcg ctcgtactgc tccacgatgg tgtagtcctc gttgtgggag
      421 gtgatgtcca gcttggcgtc cacgtagtag tagccgggca gctgcacggg cttcttggcc
      481 atgtagatgg acttgaactc caccaggtag tggccgccgt ccttcagctt cagggccttg
      541 tgggtctcgc ccttcagcac gccgtcgcgg gggtacaggc gctcggtgga ggcctcccag
      601 cccatggtct tcttctgcat cacggggccg tcggagggga agttcacgcc gatgaacttc
      661 accttgtaga tgaagcagcc gtcctgcagg gaggagtcct gggtcacggt cgccacgccg
      721 ccgtcctcga agttcatcac gcgctcccac ttgaagccct cggggaagga cagcttcttg
      781 tagtcgggga tgtcggcggg gtgcttcacg tacaccttgg agccgtactg gaactggggg
      841 gacaggatgt cccaggcgaa gggcaggggg ccgcccttgg tcaccttcag cttcacggtg
      901 ttgtggccct cgtaggggcg gccctcgccc tcgccctcga tctcgaactc gtggccgttc
      961 acggtgccct ccatgcgcac cttgaagcgc atgaactcgg tgatgacgtt ctcggaggag
     1021 gccatggtgg cgaccggtgg atccttgcta gcagcttgaa ttctcgactg cgctctcagg
     1081 cacgacacga ctcctccgct gcccaccgca gactgaggca gcgctgagtc gccggcgccg
     1141 cagcgcagat ggtcgcgccc gtgcccccct atctcgcgcc tcgcgtggtg cggtccggct
     1201 gggccggcgg cggcgcggac gcgaccaagg tggccgggaa ggggagtttg cgggggaccg
     1261 gcgagtgacg tcagcgcgcc ttcagtgctg aggcggcggt ggcgcgcgcc gccaggcggg
     1321 ggcgaaggca ctgtccgcgg tgctgaagct ggcagtgcgc acgcgcctcg ccgcatcctg
     1381 tttcccctcc ccctctctga taggggatgc gcaatttggg gaatgggggt tgggtgcttg
     1441 tccagtgggt cggggtcggt cgtcaggtag gcacccccac cccgcctcat cctggtccta
     1501 aaacccactt gcactcatac gcagggccct ctgcaggaat tcgaacgctg acgtcatcaa
     1561 cccgctccaa ggaatcgcgg gcccagtgtc actaggcggg aacacccagc gcgcgtgcgc
     1621 cctggcagga agatggctgt gagggacagg ggagtggcgc cctgcaatat ttgcatgtcg
     1681 ctatgtgttc tgggaaatca ccataaacgt gaaatgtctt tggatttggg aatcttataa
     1741 gttctgtatg agaccacaga tccccaagct gaccctgaag ttcattcaag agatgaactt
     1801 cagggtcagc tttttttgga aaagctttag tttcttcatc tgtaaaatag aaatgataat
     1861 ggtgtatacg tcataggact gttgtgagat ttaaatgatt catgcatgaa atttcttaga
     1921 agcatgcctc acattatagc attcatttca ctaagtgcta tttttctttc cttttgacta
     1981 tgcccacggc atgcaaaaat tccagggcca gggatcaaac ccacctgtgc cacagaagtg
     2041 ggaatgctgg atccttaatc cgggagccac cagaaaacac cagtgttgac tattatcatt
     2101 acacaccagc cagttgtatt tgtatatgac tgatttctag aatgatggaa tctcacatca
     2161 taataacctg aggatttact tgttacctca agtgggaagt tcaaggatgc acctgattct
     2221 ttgggaggct ccatcagtgg ggccaggggt tctctgagtt ttctgtcagg ctccctccag
     2281 gtggtaaaat ggcagctggc agcctcatat caacatcccc cgatcaattc ctgtggacaa
     2341 caaccatctt ttccacctac tgtggcaaaa agtaaccaag gaagacactg attgacccag
     2401 tgaggggcac ataccaatcc ctgtggcttg agggtggaga ggtgacaggt cagctttatt
     2461 gtattcacaa ccaggaagca gagaagggaa caacaaatga ccactatagt tcctacgccc
     2521 tttctatatg ctgcagctga gtccatctgg cttcctgttt ccattgcaaa ggcccactga
     2581 gttttcagct caccatcctc tctgacagtc caagtcagag cctgacataa ttggccccca
     2641 ttctataatg aatgcaccat aattagccta ggttagtaaa atgcaacaag taaaatacac
     2701 tcagtgcggc aatttttata aatctttatc cctatggcta tttcaaattc agaagaccag
     2761 actagtccaa taactaaaag ccccacacat atgtggtaaa accatcctct gatagatgta
     2821 acattacaca gtggggccct gcagtccaca gtgaaagaga aataggtgag gccagaacat
     2881 acactttctt gtttcagctc tactaatagt tcaagaaaat aaagtctctt agtctcacag
     2941 atactaaaga tcccgtattt ctaaaacaga ggttgaagca tccaggtaac cgactacatg
     3001 gaatttttgt gaagagtaaa atgaactgtc atatgtagaa gagattagta aatataataa
     3061 aaataattag caatattatc tctaataatt aaattgttct ttgtttccta atcctcttaa
     3121 acacagaaat gatttcttct ctttggctta gatttctctt ctgttctcag aatagttgtc
     3181 acttgacacc ctgttgtcac ctcttataca aagaactcca ccttctcttg tcttttctga
     3241 aacaaccatg catttgcttt ctctatttgt cacactcatc tccctttttt ctctcttaga
     3301 tttttttttg tttccatttt aattggtcat tatcccctca ctggtgataa aagcccaccc
     3361 ttctttttta ttcaccatgg aaccactgta gtatgctggt tggaccattc aaatcttgac
     3421 ttcaccttgg acctttcact tgattctgtc tattcaccat cgttgcacag gagagttctg
     3481 tctgtgtttt gtttctgagt ctggcaccac tctgaaggtc taactttgta gggagtctct
     3541 ctccagataa tttaaaaagt tgaattatcc ctaggaagta gctcaagaac ttgaatagcc
     3601 tctcaaattc cactcactgg attaatgctt aaaaaaataa tttttgtggc tcagtggaat
     3661 tgaatctgac tagtatccat gaggattcag gttcaatccc tagccttgct cagtgggtcc
     3721 aggatccggt gttgctgtga gctatagtgt aggttgcaga cacagcctgg attctgtgtt
     3781 gctgtggtat aggctggcag ctatagcact gattcaaccc tctaggagga actttcatat
     3841 gtcgtgtgtg tggcccccca aaaataacaa caacgacaac taaaacaata aagtaaaatg
     3901 tgctttaata aaaataataa ataaggcatt agcctgagga gctgatggca ccacctgcct
     3961 tcagtacaag gattcaacac caacgtgtgg gtgtttggga agagttgatc agaccgacac
     4021 tgaagctgga aataagcagc agcagcagta gtaccacaaa gcctgggaaa cacacccaca
     4081 aaatgacagt gtgcggaccg agcggccgca ggaaccccta gtgatggagt tggccactcc
     4141 ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg
     4201 ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat
     4261 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgtc aaagcaacca
     4321 tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     4381 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     4441 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     4501 tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt
     4561 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     4621 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcgggcta ttcttttgat
     4681 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     4741 tttaacgcga attttaacaa aatattaacg tttacaattt tatggtgcac tctcagtaca
     4801 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
     4861 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     4921 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc
     4981 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     5041 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     5101 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     5161 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     5221 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     5281 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     5341 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     5401 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     5461 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     5521 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     5581 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     5641 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     5701 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     5761 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     5821 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     5881 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     5941 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     6001 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     6061 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     6121 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     6181 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     6241 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     6301 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     6361 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     6421 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     6481 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     6541 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     6601 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     6661 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     6721 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     6781 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     6841 cacatgtcct gcaggcagct gcgcgctcgc tcgctcactg aggccgcccg ggcaaagccc
     6901 gggcgtcggg cgacctttgg tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga
     6961 gtggccaact ccatcactag gggttcctgc
//
