LOCUS       40924_3266        7187 bp DNA     circular SYN 17-DEC-2018
DEFINITION  SiRNA vector pAAV9(5)-CMV-EGFP-CytB-AS-ohneNot, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7187)
  AUTHORS   Michel U, Malik I, Ebert S, Bahr M, Kugler S.
  TITLE     Long-term in vivo and in vitro AAV-2-mediated RNA interference in 
            rat retinal ganglion cells and cultured primary neurons
  JOURNAL   Biochem. Biophys. Res. Commun. 326 (2), 307-312 (2005)
  PUBMED    15582578
REFERENCE   2  (bases 1 to 7187)
  AUTHORS   Michel U, Malik I, Kuegler S.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUN-2004) Neurology, University of Goettingen, Waldweg
            33, Goettingen 37073, Germany
REFERENCE   3  (bases 1 to 7187)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7187)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem. 
            Biophys. Res. Commun."; date: "2005"; volume: "326"; issue: "2"; 
            pages: "307-312"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (01-JUN-2004) Neurology, University of Goettingen, Waldweg 33, 
            Goettingen 37073, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7187
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     polyA_signal    complement(194..328)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     intron          complement(344..477)
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     CDS             complement(492..1208)
                     /label=EGFP
                     /note="enhanced GFP"
     misc_feature    complement(1227..1246)
                     /note="MCS; multiple cloning site"
     promoter        complement(1249..1777)
                     /label=mCMV promoter
                     /note="mouse cytomegalovirus (CMV) immediate early
                     promoter"
     primer_bind     1779..1795
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1815..2029
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     misc_feature    2031..2162
                     /note="Cytb-AS; partial human cytochrome B antisense
                     sequence"
     gene            complement(2167..4430)
                     /gene="9(5)"
                     /label=9(5)
                     /note="partial sequence from the non-coding porcine RNA UM
                     9(5)"
     repeat_region   4450..4590
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4665..5120
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5402..5506
                     /label=AmpR promoter
     CDS             5507..6364
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      6538..7126
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgatcgaa ttcctgcagc ccgggggatc
      181 ttcgatgcta gacgatccag acatgataag atacattgat gagtttggac aaaccacaac
      241 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
      301 aaccattata agctgcaata aacaagttgc tcgaagtcga cacctgtgga gagaaaggca
      361 aagtggatgt cagtaagacc aataggtgcc tatcagaaac gcaagagtct tctctgtctc
      421 gacaagccca gtttctatgt ggtctcctta aacctgtctt gtaaccttga tacttacctg
      481 ccgccgcttt acttgtacag ctcgtccatg ccgagagtga tcccggcggc ggtcacgaac
      541 tccagcagga ccatgtgatc gcgcttctcg ttggggtctt tgctcagggc ggactgggtg
      601 ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt gttctgctgg
      661 tagtggtcgg cgagctgcac gctgccgtcc tcgatgttgt ggcggatctt gaagttcacc
      721 ttgatgccgt tcttctgctt gtcggccatg atatagacgt tgtggctgtt gtagttgtac
      781 tccagcttgt gccccaggat gttgccgtcc tccttgaagt cgatgccctt cagctcgatg
      841 cggttcacca gggtgtcgcc ctcgaacttc acctcggcgc gggtcttgta gttgccgtcg
      901 tccttgaaga agatggtgcg ctcctggacg tagccttcgg gcatggcgga cttgaagaag
      961 tcgtgctgct tcatgtggtc ggggtagcgg ctgaagcact gcacgccgta ggtcagggtg
     1021 gtcacgaggg tgggccaggg cacgggcagc ttgccggtgg tgcagatgaa cttcagggtc
     1081 agcttgccgt aggtggcatc gccctcgccc tcgccggaca cgctgaactt gtggccgttt
     1141 acgtcgccgt ccagctcgac caggatgggc accaccccgg tgaacagctc ctcgcccttg
     1201 ctcaccatgg tggcgaccgg tggatccttg ctagcagctt gaattcgatc tgcgttctac
     1261 ggtggtcaga ccgaagactg cgacggtacc gacgctggtc gcgcctctta tacccacgta
     1321 gaacgcagct cagccaatag aatgagtgcc aatatggaat ttccagggga aaaccggggc
     1381 ggtgttacgt tttggctgcc ctttcacttc ccattgacgt gtattggctc gagaacggta
     1441 ctttcccatt aatcagctat gggaaagtac cgtttaaagg tcacgttgca ttagtttcaa
     1501 tagcccattg acgtcaatgg tgggaaagta catggcgttt taattaaatg gctggaaaaa
     1561 cccaatgact cacccctatt gaccttatgt acgtgccaat aatgggaaaa acccattgac
     1621 tcacccccta ttgacctttt gtactgggca aaacccaatg gaaagtccct attgactcag
     1681 tgtacttggc tccaatggga ctttcctgtt gattcacccc tattgacctt atgtactggg
     1741 caaaacccat tggaaagtcc ctaatgactc agtatatctc tagaactagt ggatcccccg
     1801 ggctgcagga attcgaacgc tgacgtcatc aacccgctcc aaggaatcgc gggcccagtg
     1861 tcactaggcg ggaacaccca gcgcgcgtgc gccctggcag gaagatggct gtgagggaca
     1921 ggggagtggc gccctgcaat atttgcatgt cgctatgtgt tctgggaaat caccataaac
     1981 gtgaaatgtc tttggatttg ggaatcttat aagttctgta tgagaccaca gatctcccaa
     2041 tgtatgggat ggcggatagt aagtttgtaa ttactgtggc ccctcagaat gatatttggc
     2101 ctcacgggag gacatagcct atgaaggctg ttgctatagt tgcaagcagg aggataatgc
     2161 caagctttag tttcttcatc tgtaaaatag aaatgataat ggtgtatacg tcataggact
     2221 gttgtgagat ttaaatgatt catgcatgaa atttcttaga agcatgcctc acattatagc
     2281 attcatttca ctaagtgcta tttttctttc cttttgacta tgcccacggc atgcaaaaat
     2341 tccagggcca gggatcaaac ccacctgtgc cacagaagtg ggaatgctgg atccttaatc
     2401 cgggagccac cagaaaacac cagtgttgac tattatcatt acacaccagc cagttgtatt
     2461 tgtatatgac tgatttctag aatgatggaa tctcacatca taataacctg aggatttact
     2521 tgttacctca agtgggaagt tcaaggatgc acctgattct ttgggaggct ccatcagtgg
     2581 ggccaggggt tctctgagtt ttctgtcagg ctccctccag gtggtaaaat ggcagctggc
     2641 agcctcatat caacatcccc cgatcaattc ctgtggacaa caaccatctt ttccacctac
     2701 tgtggcaaaa agtaaccaag gaagacactg attgacccag tgaggggcac ataccaatcc
     2761 ctgtggcttg agggtggaga ggtgacaggt cagctttatt gtattcacaa ccaggaagca
     2821 gagaagggaa caacaaatga ccactatagt tcctacgccc tttctatatg ctgcagctga
     2881 gtccatctgg cttcctgttt ccattgcaaa ggcccactga gttttcagct caccatcctc
     2941 tctgacagtc caagtcagag cctgacataa ttggccccca ttctataatg aatgcaccat
     3001 aattagccta ggttagtaaa atgcaacaag taaaatacac tcagtgcggc aatttttata
     3061 aatctttatc cctatggcta tttcaaattc agaagaccag actagtccaa taactaaaag
     3121 ccccacacat atgtggtaaa accatcctct gatagatgta acattacaca gtggggccct
     3181 gcagtccaca gtgaaagaga aataggtgag gccagaacat acactttctt gtttcagctc
     3241 tactaatagt tcaagaaaat aaagtctctt agtctcacag atactaaaga tcccgtattt
     3301 ctaaaacaga ggttgaagca tccaggtaac cgactacatg gaatttttgt gaagagtaaa
     3361 atgaactgtc atatgtagaa gagattagta aatataataa aaataattag caatattatc
     3421 tctaataatt aaattgttct ttgtttccta atcctcttaa acacagaaat gatttcttct
     3481 ctttggctta gatttctctt ctgttctcag aatagttgtc acttgacacc ctgttgtcac
     3541 ctcttataca aagaactcca ccttctcttg tcttttctga aacaaccatg catttgcttt
     3601 ctctatttgt cacactcatc tccctttttt ctctcttaga tttttttttg tttccatttt
     3661 aattggtcat tatcccctca ctggtgataa aagcccaccc ttctttttta ttcaccatgg
     3721 aaccactgta gtatgctggt tggaccattc aaatcttgac ttcaccttgg acctttcact
     3781 tgattctgtc tattcaccat cgttgcacag gagagttctg tctgtgtttt gtttctgagt
     3841 ctggcaccac tctgaaggtc taactttgta gggagtctct ctccagataa tttaaaaagt
     3901 tgaattatcc ctaggaagta gctcaagaac ttgaatagcc tctcaaattc cactcactgg
     3961 attaatgctt aaaaaaataa tttttgtggc tcagtggaat tgaatctgac tagtatccat
     4021 gaggattcag gttcaatccc tagccttgct cagtgggtcc aggatccggt gttgctgtga
     4081 gctatagtgt aggttgcaga cacagcctgg attctgtgtt gctgtggtat aggctggcag
     4141 ctatagcact gattcaaccc tctaggagga actttcatat gtcgtgtgtg tggcccccca
     4201 aaaataacaa caacgacaac taaaacaata aagtaaaatg tgctttaata aaaataataa
     4261 ataaggcatt agcctgagga gctgatggca ccacctgcct tcagtacaag gattcaacac
     4321 caacgtgtgg gtgtttggga agagttgatc agaccgacac tgaagctgga aataagcagc
     4381 agcagcagta gtaccacaaa gcctgggaaa cacacccaca aaatgacagt gtgcggaccg
     4441 agcggccgca ggaaccccta gtgatggagt tggccactcc ctctctgcgc gctcgctcgc
     4501 tcactgaggc cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag
     4561 tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc
     4621 atctgtgcgg tatttcacac cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg
     4681 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     4741 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     4801 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     4861 cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac
     4921 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     4981 tggaacaaca ctcaacccta tctcgggcta ttcttttgat ttataaggga ttttgccgat
     5041 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     5101 aatattaacg tttacaattt tatggtgcac tctcagtaca atctgctctg atgccgcata
     5161 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
     5221 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
     5281 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata
     5341 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     5401 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     5461 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     5521 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     5581 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     5641 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     5701 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     5761 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     5821 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     5881 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     5941 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     6001 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     6061 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     6121 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     6181 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     6241 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     6301 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     6361 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     6421 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     6481 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     6541 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     6601 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     6661 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     6721 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     6781 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     6841 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     6901 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     6961 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     7021 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     7081 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     7141 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgt
//
