LOCUS       40924_3261        7169 bp DNA     circular SYN 17-DEC-2018
DEFINITION  SiRNA vector pAAV9(5)-CMV-EGFP-CytB-AS-ohneBgl, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7169)
  AUTHORS   Michel U, Malik I, Ebert S, Bahr M, Kugler S.
  TITLE     Long-term in vivo and in vitro AAV-2-mediated RNA interference in 
            rat retinal ganglion cells and cultured primary neurons
  JOURNAL   Biochem. Biophys. Res. Commun. 326 (2), 307-312 (2005)
  PUBMED    15582578
REFERENCE   2  (bases 1 to 7169)
  AUTHORS   Michel U, Malik I, Kuegler S.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUN-2004) Neurology, University of Goettingen, Waldweg
            33, Goettingen 37073, Germany
REFERENCE   3  (bases 1 to 7169)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7169)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem. 
            Biophys. Res. Commun."; date: "2005"; volume: "326"; issue: "2"; 
            pages: "307-312"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (01-JUN-2004) Neurology, University of Goettingen, Waldweg 33, 
            Goettingen 37073, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7169
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    complement(51..185)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     intron          complement(201..334)
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     CDS             complement(349..1065)
                     /label=EGFP
                     /note="enhanced GFP"
     misc_feature    complement(1084..1103)
                     /note="MCS'; multiple cloning site"
     promoter        complement(1106..1634)
                     /label=mCMV promoter
                     /note="mouse cytomegalovirus (CMV) immediate early
                     promoter"
     primer_bind     1636..1652
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1672..1886
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     misc_feature    1888..2001
                     /note="Cytb-AS; partial human cytochrome B antisense
                     sequence"
     gene            complement(2006..4269)
                     /gene="9(5)"
                     /label=9(5)
                     /note="partial sequence from the non-coding porcine RNA UM
                     9(5)"
     repeat_region   4289..4429
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4504..4959
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5241..5345
                     /label=AmpR promoter
     CDS             5346..6203
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      6377..6965
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     repeat_region   7027..7167
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
ORIGIN
        1 ggccgcacgc gatcgaattc ctgcagcccg ggggatcttc gatgctagac gatccagaca
       61 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
      121 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
      181 aagttgctcg aagtcgacac ctgtggagag aaaggcaaag tggatgtcag taagaccaat
      241 aggtgcctat cagaaacgca agagtcttct ctgtctcgac aagcccagtt tctatgtggt
      301 ctccttaaac ctgtcttgta accttgatac ttacctgccg ccgctttact tgtacagctc
      361 gtccatgccg agagtgatcc cggcggcggt cacgaactcc agcaggacca tgtgatcgcg
      421 cttctcgttg gggtctttgc tcagggcgga ctgggtgctc aggtagtggt tgtcgggcag
      481 cagcacgggg ccgtcgccga tgggggtgtt ctgctggtag tggtcggcga gctgcacgct
      541 gccgtcctcg atgttgtggc ggatcttgaa gttcaccttg atgccgttct tctgcttgtc
      601 ggccatgata tagacgttgt ggctgttgta gttgtactcc agcttgtgcc ccaggatgtt
      661 gccgtcctcc ttgaagtcga tgcccttcag ctcgatgcgg ttcaccaggg tgtcgccctc
      721 gaacttcacc tcggcgcggg tcttgtagtt gccgtcgtcc ttgaagaaga tggtgcgctc
      781 ctggacgtag ccttcgggca tggcggactt gaagaagtcg tgctgcttca tgtggtcggg
      841 gtagcggctg aagcactgca cgccgtaggt cagggtggtc acgagggtgg gccagggcac
      901 gggcagcttg ccggtggtgc agatgaactt cagggtcagc ttgccgtagg tggcatcgcc
      961 ctcgccctcg ccggacacgc tgaacttgtg gccgtttacg tcgccgtcca gctcgaccag
     1021 gatgggcacc accccggtga acagctcctc gcccttgctc accatggtgg cgaccggtgg
     1081 atccttgcta gcagcttgaa ttcgatctgc gttctacggt ggtcagaccg aagactgcga
     1141 cggtaccgac gctggtcgcg cctcttatac ccacgtagaa cgcagctcag ccaatagaat
     1201 gagtgccaat atggaatttc caggggaaaa ccggggcggt gttacgtttt ggctgccctt
     1261 tcacttccca ttgacgtgta ttggctcgag aacggtactt tcccattaat cagctatggg
     1321 aaagtaccgt ttaaaggtca cgttgcatta gtttcaatag cccattgacg tcaatggtgg
     1381 gaaagtacat ggcgttttaa ttaaatggct ggaaaaaccc aatgactcac ccctattgac
     1441 cttatgtacg tgccaataat gggaaaaacc cattgactca ccccctattg accttttgta
     1501 ctgggcaaaa cccaatggaa agtccctatt gactcagtgt acttggctcc aatgggactt
     1561 tcctgttgat tcacccctat tgaccttatg tactgggcaa aacccattgg aaagtcccta
     1621 atgactcagt atatctctag aactagtgga tcccccgggc tgcaggaatt cgaacgctga
     1681 cgtcatcaac ccgctccaag gaatcgcggg cccagtgtca ctaggcggga acacccagcg
     1741 cgcgtgcgcc ctggcaggaa gatggctgtg agggacaggg gagtggcgcc ctgcaatatt
     1801 tgcatgtcgc tatgtgttct gggaaatcac cataaacgtg aaatgtcttt ggatttggga
     1861 atcttataag ttctgtatga gaccacagat ccccaatgca tgggatggcg gatagtaagt
     1921 ttgtaattac tgtggcccct cagaatgata tttggcctca cgggaggaca tagcctatga
     1981 aggctgttgc ttttttggaa aagctttagt ttcttcatct gtaaaataga aatgataatg
     2041 gtgtatacgt cataggactg ttgtgagatt taaatgattc atgcatgaaa tttcttagaa
     2101 gcatgcctca cattatagca ttcatttcac taagtgctat ttttctttcc ttttgactat
     2161 gcccacggca tgcaaaaatt ccagggccag ggatcaaacc cacctgtgcc acagaagtgg
     2221 gaatgctgga tccttaatcc gggagccacc agaaaacacc agtgttgact attatcatta
     2281 cacaccagcc agttgtattt gtatatgact gatttctaga atgatggaat ctcacatcat
     2341 aataacctga ggatttactt gttacctcaa gtgggaagtt caaggatgca cctgattctt
     2401 tgggaggctc catcagtggg gccaggggtt ctctgagttt tctgtcaggc tccctccagg
     2461 tggtaaaatg gcagctggca gcctcatatc aacatccccc gatcaattcc tgtggacaac
     2521 aaccatcttt tccacctact gtggcaaaaa gtaaccaagg aagacactga ttgacccagt
     2581 gaggggcaca taccaatccc tgtggcttga gggtggagag gtgacaggtc agctttattg
     2641 tattcacaac caggaagcag agaagggaac aacaaatgac cactatagtt cctacgccct
     2701 ttctatatgc tgcagctgag tccatctggc ttcctgtttc cattgcaaag gcccactgag
     2761 ttttcagctc accatcctct ctgacagtcc aagtcagagc ctgacataat tggcccccat
     2821 tctataatga atgcaccata attagcctag gttagtaaaa tgcaacaagt aaaatacact
     2881 cagtgcggca atttttataa atctttatcc ctatggctat ttcaaattca gaagaccaga
     2941 ctagtccaat aactaaaagc cccacacata tgtggtaaaa ccatcctctg atagatgtaa
     3001 cattacacag tggggccctg cagtccacag tgaaagagaa ataggtgagg ccagaacata
     3061 cactttcttg tttcagctct actaatagtt caagaaaata aagtctctta gtctcacaga
     3121 tactaaagat cccgtatttc taaaacagag gttgaagcat ccaggtaacc gactacatgg
     3181 aatttttgtg aagagtaaaa tgaactgtca tatgtagaag agattagtaa atataataaa
     3241 aataattagc aatattatct ctaataatta aattgttctt tgtttcctaa tcctcttaaa
     3301 cacagaaatg atttcttctc tttggcttag atttctcttc tgttctcaga atagttgtca
     3361 cttgacaccc tgttgtcacc tcttatacaa agaactccac cttctcttgt cttttctgaa
     3421 acaaccatgc atttgctttc tctatttgtc acactcatct cccttttttc tctcttagat
     3481 ttttttttgt ttccatttta attggtcatt atcccctcac tggtgataaa agcccaccct
     3541 tcttttttat tcaccatgga accactgtag tatgctggtt ggaccattca aatcttgact
     3601 tcaccttgga cctttcactt gattctgtct attcaccatc gttgcacagg agagttctgt
     3661 ctgtgttttg tttctgagtc tggcaccact ctgaaggtct aactttgtag ggagtctctc
     3721 tccagataat ttaaaaagtt gaattatccc taggaagtag ctcaagaact tgaatagcct
     3781 ctcaaattcc actcactgga ttaatgctta aaaaaataat ttttgtggct cagtggaatt
     3841 gaatctgact agtatccatg aggattcagg ttcaatccct agccttgctc agtgggtcca
     3901 ggatccggtg ttgctgtgag ctatagtgta ggttgcagac acagcctgga ttctgtgttg
     3961 ctgtggtata ggctggcagc tatagcactg attcaaccct ctaggaggaa ctttcatatg
     4021 tcgtgtgtgt ggccccccaa aaataacaac aacgacaact aaaacaataa agtaaaatgt
     4081 gctttaataa aaataataaa taaggcatta gcctgaggag ctgatggcac cacctgcctt
     4141 cagtacaagg attcaacacc aacgtgtggg tgtttgggaa gagttgatca gaccgacact
     4201 gaagctggaa ataagcagca gcagcagtag taccacaaag cctgggaaac acacccacaa
     4261 aatgacagtg tgcggaccga gcggccgcag gaacccctag tgatggagtt ggccactccc
     4321 tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc
     4381 tttgcccggg cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg gcgcctgatg
     4441 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgtca aagcaaccat
     4501 agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     4561 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     4621 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     4681 ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg
     4741 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     4801 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcgggctat tcttttgatt
     4861 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     4921 ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt atggtgcact ctcagtacaa
     4981 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
     5041 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     5101 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg
     5161 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     5221 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     5281 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     5341 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     5401 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     5461 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     5521 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     5581 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     5641 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     5701 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     5761 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     5821 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     5881 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     5941 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     6001 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     6061 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     6121 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     6181 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     6241 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     6301 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     6361 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     6421 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     6481 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     6541 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     6601 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     6661 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     6721 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     6781 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     6841 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     6901 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     6961 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     7021 acatgtcctg caggcagctg cgcgctcgct cgctcactga ggccgcccgg gcaaagcccg
     7081 ggcgtcgggc gacctttggt cgcccggcct cagtgagcga gcgagcgcgc agagagggag
     7141 tggccaactc catcactagg ggttcctgc
//
