LOCUS       V009661                 7934 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009661
VERSION     V009661
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7934)
  AUTHORS   Adachi K, Enoki T, Kawano Y, Veraz M, Nakai H.
  TITLE     Drawing a high-resolution functional map of adeno-associated virus
            capsid by massively parallel sequencing
  JOURNAL   Nat Commun 5, 3075 (2014)
   PUBMED   24435020
REFERENCE   2  (bases 1 to 7934)
  AUTHORS   Nakai H.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2013) Department of Molecular and Medical
            Genetics, Oregon Health and Science University, 3181 SW Sam Jackson
            Park Road, Portland, OR 97239, USA
REFERENCE   3  (bases 1 to 7934)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7934)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Nat Commun
            5, 3075 (2014)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (07-MAY-2013) Department of Molecular and Medical Genetics, Oregon
            Health and Science University, 3181 SW Sam Jackson Park Road,
            Portland, OR 97239, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7934
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..141
                     /label="AAV2 ITR"
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
     CDS             317..2179
                     /gene="Rep78"
                     /label="Protein Rep78"
                     /note="Protein Rep78 from Adeno-associated virus 2 (isolate
                     Srivastava/1982). Accession#: Q89268"
     CDS             2199..4403
                     /gene="VP1"
                     /label="Capsid protein VP1"
                     /note="Capsid protein VP1 from Adeno-associated virus 2
                     (isolate Srivastava/1982). Accession#: P03135"
     gap             4528..4539
                     /estimated_length=12
     gap             4560..4571
                     /estimated_length=12
     repeat_region   4677..4817
                     /label="AAV2 ITR"
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
     primer_bind     complement(4827..4843)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      5056..5511
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5793..5897
                     /label="AmpR promoter"
     CDS             5898..6755
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      6929..7517
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    7805..7826
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        7841..7871
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    7879..7895
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7903..7919
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tggaggggtg gagtcgtgac gtgaattacg tcatagggtt
      181 agggaggtcc tgtattagag gtcacgtgag tgttttgcga cattttgcga caccatgtgg
      241 tcacgctggg tatttaagcc cgagtgagca cgcagggtct ccattttgaa gcgggaggtt
      301 tgaacgcgca gccgccatgc cggggtttta cgagattgtg attaaggtcc ccagcgacct
      361 tgacgggcat ctgcccggca tttctgacag ctttgtgaac tgggtggccg agaaggaatg
      421 ggagttgccg ccagattctg acatggatct gaatctgatt gagcaggcac ccctgaccgt
      481 ggccgagaag ctgcagcgcg actttctgac ggaatggcgc cgtgtgagta aggccccgga
      541 ggcccttttc tttgtgcaat ttgagaaggg agagagctac ttccacatgc acgtgctcgt
      601 ggaaaccacc ggggtgaaat ccatggtttt gggacgtttc ctgagtcaga ttcgcgaaaa
      661 actgattcag agaatttacc gcgggatcga gccgactttg ccaaactggt tcgcggtcac
      721 aaagaccaga aatggcgccg gaggcgggaa caaggtggtg gatgagtgct acatccccaa
      781 ttacttgctc cccaaaaccc agcctgagct ccagtgggcg tggactaata tggaacagta
      841 tttaagcgcc tgtttgaatc tcacggagcg taaacggttg gtggcgcagc atctgacgca
      901 cgtgtcgcag acgcaggagc agaacaaaga gaatcagaat cccaattctg atgcgccggt
      961 gatcagatca aaaacttcag ccaggtacat ggagctggtc gggtggctcg tggacaaggg
     1021 gattacctcg gagaagcagt ggatccagga ggaccaggcc tcatacatct ccttcaatgc
     1081 ggcctccaac tcgcggtccc aaatcaaggc tgccttggac aatgcgggaa agattatgag
     1141 cctgactaaa accgcccccg actacctggt gggccagcag cccgtggagg acatttccag
     1201 caatcggatt tataaaattt tggaactaaa cgggtacgat ccccaatatg cggcttccgt
     1261 ctttctggga tgggccacga aaaagttcgg caagaggaac accatctggc tgtttgggcc
     1321 tgcaactacc gggaagacca acatcgcgga ggccatagcc cacactgtgc ccttctacgg
     1381 gtgcgtaaac tggaccaatg agaactttcc cttcaacgac tgtgtcgaca agatggtgat
     1441 ctggtgggag gaggggaaga tgaccgccaa ggtcgtggag tcggccaaag ccattctcgg
     1501 aggaagcaag gtgcgcgtgg accagaaatg caagtcctcg gcccagatag acccgactcc
     1561 cgtgatcgtc acctccaaca ccaacatgtg cgccgtgatt gacgggaact caacgacctt
     1621 cgaacaccag cagccgttgc aagaccggat gttcaaattt gaactcaccc gccgtctgga
     1681 tcatgacttt gggaaggtca ccaagcagga agtcaaagac tttttccggt gggcaaagga
     1741 tcacgtggtt gaggtggagc atgaattcta cgtcaaaaag ggtggagcca agaaaagacc
     1801 cgcccccagt gacgcagata taagtgagcc caaacgggtg cgcgagtcag ttgcgcagcc
     1861 atcgacgtca gacgcggaag cttcgatcaa ctacgcagac aggtaccaaa acaaatgttc
     1921 tcgtcacgtg ggcatgaatc tgatgctgtt tccctgcaga caatgcgaga gaatgaatca
     1981 gaattcaaat atctgcttca ctcacggaca gaaagactgt ttagagtgct ttcccgtgtc
     2041 agaatctcaa cccgtttctg tcgtcaaaaa ggcgtatcag aaactgtgct acattcatca
     2101 tatcatggga aaggtgccag acgcttgcac tgcatgcgat ctggtcaatg tggatttgga
     2161 tgactgcatc tttgaacaat aaatgattta aatcaggtat ggctgccgat ggttatcttc
     2221 cagattggct cgaggacact ctctctgaag gaataagaca gtggtggaag ctcaaacctg
     2281 gcccaccacc accaaagccc gcagagcggc ataaggacga cagcaggggt cttgtgcttc
     2341 ctgggtacaa gtacctcgga cccttcaacg gactcgacaa gggagagccg gtcaacgagg
     2401 cagacgccgc ggccctcgag cacgacaaag cctacgaccg gcagctcgac agcggagaca
     2461 acccgtacct caagtacaac cacgccgacg cggagtttca ggagcgcctt aaagaagata
     2521 cgtcttttgg gggcaacctc ggacgagcag tcttccaggc gaaaaagagg gttcttgaac
     2581 ctctgggcct ggttgaggaa cctgttaaga cggctccggg aaaaaagagg ccggtagagc
     2641 actctcctgt ggagccagac tcctcctccg gaaccggaaa ggcgggccag cagcctgcaa
     2701 gaaaaagatt gaattttggt cagactggag acgcagactc agtacctgac ccccagcctc
     2761 tcggacagcc accagcagcc ccctctggtc tgggaactaa tacgatggct acaggcagtg
     2821 gcgcaccaat ggcagacaat aacgagggcg ccgacggagt gggtaattcc tcgggaaatt
     2881 ggcattgcga ttccacatgg atgggcgaca gagtcatcac caccagcacc cgaacctggg
     2941 ccctgcccac ctacaacaac cacctctaca aacaaatttc cagccaatca ggagcctcga
     3001 acgacaatca ctactttggc tacagcaccc cttgggggta ttttgacttc aacagattcc
     3061 actgccactt ttcaccacgt gactggcaaa gactcatcaa caacaactgg ggattccgac
     3121 ccaagagact caacttcaag ctctttaaca ttcaagtcaa agaggtcacg cagaatgacg
     3181 gtacgacgac gattgccaat aaccttacca gcacggttca ggtgtttact gactcggagt
     3241 accagctccc gtacgtcctc ggctcggcgc atcaaggatg cctcccgccg ttcccagcag
     3301 acgtcttcat ggtgccacag tatggatacc tcaccctgaa caacgggagt caggcagtag
     3361 gacgatcttc attttactgc ctggagtact ttccttctca gatgctgcgt accggaaaca
     3421 actttacctt cagctacact tttgaggacg ttcctttcca cagcagctac gctcacagcc
     3481 agagtctgga ccgtctcatg aatcctctca tcgaccagta cctgtattac ttgagcagaa
     3541 caaacactcc aagtggaacc accacgcagt caaggcttca gttttctcag gccggagcga
     3601 gtgacattcg ggaccagtct agaaactggc ttcctggacc ctgttaccgc cagcagcgag
     3661 tatcaaagac atctgcggat aacaacaaca gtgaatactc gtggactgga gctaccaagt
     3721 accacctcaa tggcagagac tctctggtga atccgggccc ggccatggca agccacaagg
     3781 acgatgaaga aaagtttttt cctcagagcg gggttctcat ctttgggaag caaggatcag
     3841 agaaaacaaa tgtggacatt gaaaaggtca tgattacaga cgaagaggaa atcaggacaa
     3901 ccaatcccgt ggctacggag cagtatggtt ctgtatctac caacctccag gaaggcaaca
     3961 gacaagcagc tacggccgat gtcaacacac aaggcgttct tccaggcatg gtctggcagg
     4021 acagagatgt gtaccttcag gggcccatct gggcaaagat tccacacacg gacggacatt
     4081 ttcacccctc tcccctcatg ggtggattcg gacttaaaca ccctcctcca cagattctca
     4141 tcaagaacac cccggtacct gcgaatcctt cgaccacctt cagtgcggca aagtttgctt
     4201 ccttcatcac acagtactcc acgggacagg tcagcgtgga gatcgagtgg gagctgcaga
     4261 aggaaaacag caaacgctgg aatcccgaaa ttcagtacac ttccaactac aacaagtctg
     4321 ttaatgtgga ctttactgtg gacactaatg gcgtgtattc agagcctcgc cccattggca
     4381 ccagatacct gactcgtaat ctgtaattgc ttgttaatca ataaaccgtt taattcgttt
     4441 cagttgaact ttggtctctg cgtatttctt tcttatctag tttccatggc tacgtagatc
     4501 tacctacgta cttccgctca tgctagcnnn nnnnnnnnna cggaaatacg atgtcgggan
     4561 nnnnnnnnnn ntgtacagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     4621 gttcgtgacc gccgcctacg tagataagta gcatggcggg ttaatcatta actacaagga
     4681 acccctagtg atggagttgg ccactccctc tctgcgcgct cgctcgctca ctgaggccgg
     4741 gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc
     4801 gcgcagctgc ctgcaggcag cttggcactg gccgtcgttt tacaacgtcg tgactgggaa
     4861 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     4921 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     4981 tggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatacgt
     5041 caaagcaacc atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     5101 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     5161 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     5221 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg
     5281 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     5341 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcgggct
     5401 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     5461 tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt ttatggtgca
     5521 ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac
     5581 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     5641 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac
     5701 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     5761 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     5821 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     5881 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     5941 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     6001 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     6061 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     6121 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     6181 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     6241 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     6301 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     6361 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     6421 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     6481 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     6541 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     6601 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     6661 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     6721 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     6781 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     6841 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     6901 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     6961 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     7021 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     7081 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     7141 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     7201 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     7261 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     7321 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     7381 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     7441 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     7501 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     7561 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     7621 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     7681 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     7741 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     7801 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     7861 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     7921 atgattacga attg
//
