LOCUS       V012817                 7333 bp    DNA     circular SYN 21-AUG-2023
DEFINITION  Exported.
ACCESSION   V012817
VERSION     V012817
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7333)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7333
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     12..28
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        38..56
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     86..102
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             157..2019
                     /gene="Rep78"
                     /label="Protein Rep78"
                     /note="Protein Rep78 from Adeno-associated virus 2 (isolate
                     Srivastava/1982). Accession#: Q89268"
     CDS             4094..4105
                     /label="WELQut site"
                     /note="WELQut protease recognition and cleavage site"
     promoter        complement(4557..4575)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4596..4612)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(4620..4636)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4644..4674)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4689..4710)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4998..5586)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5760..6617)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6618..6722)
                     /label="AmpR promoter"
     rep_origin      complement(6748..7203)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 gtcacgacgt tgtaaaacga cggccagtga gcgcgcgtaa tacgactcac tatagggcga
       61 attgggtacc gggccccccc tcgatcgagg tcgacggtat cgggggagct cgcagggtct
      121 ccattttgaa gcgggaggtt tgaacgcgca gccgccatgc cggggtttta cgagattgtg
      181 attaaggtcc ccagcgacct tgacgagcat ctgcccggca tttctgacag ctttgtgaac
      241 tgggtggccg agaaggaatg ggagttgccg ccagattctg acatggatct gaatctgatt
      301 gagcaggcac ccctgaccgt ggccgagaag ctgcagcgcg actttctgac ggaatggcgc
      361 cgtgtgagta aggccccgga ggctcttttc tttgtgcaat ttgagaaggg agagagctac
      421 ttccacatgc acgtgctcgt ggaaaccacc ggggtgaaat ccatggtttt gggacgtttc
      481 ctgagtcaga ttcgcgaaaa actgattcag agaatttacc gcgggatcga gccgactttg
      541 ccaaactggt tcgcggtcac aaagaccaga aatggcgccg gaggcgggaa caaggtggtg
      601 gatgagtgct acatccccaa ttacttgctc cccaaaaccc agcctgagct ccagtgggcg
      661 tggactaata tggaacagta tttaagcgcc tgtttgaatc tcacggagcg taaacggttg
      721 gtggcgcagc atctgacgca cgtgtcgcag acgcaggagc agaacaaaga gaatcagaat
      781 cccaattctg atgcgccggt gatcagatca aaaacttcag ccaggtacat ggagctggtc
      841 gggtggctcg tggacaaggg gattacctcg gagaagcagt ggatccagga ggaccaggcc
      901 tcatacatct ccttcaatgc ggcctccaac tcgcggtccc aaatcaaggc tgccttggac
      961 aatgcgggaa agattatgag cctgactaaa accgcccccg actacctggt gggccagcag
     1021 cccgtggagg acatttccag caatcggatt tataaaattt tggaactaaa cgggtacgat
     1081 ccccaatatg cggcttccgt ctttctggga tgggccacga aaaagttcgg caagaggaac
     1141 accatctggc tgtttgggcc tgcaactacc gggaagacca acatcgcgga ggccatagcc
     1201 cacactgtgc ccttctacgg gtgcgtaaac tggaccaatg agaactttcc cttcaacgac
     1261 tgtgtcgaca agatggtgat ctggtgggag gaggggaaga tgaccgccaa ggtcgtggag
     1321 tcggccaaag ccattctcgg aggaagcaag gtgcgcgtgg accagaaatg caagtcctcg
     1381 gcccagatag acccgactcc cgtgatcgtc acctccaaca ccaacatgtg cgccgtgatt
     1441 gacgggaact caacgacctt cgaacaccag cagccgttgc aagaccggat gttcaaattt
     1501 gaactcaccc gccgtctgga tcatgacttt gggaaggtca ccaagcagga agtcaaagac
     1561 tttttccggt gggcaaagga tcacgtggtt gaggtggagc atgaattcta cgtcaaaaag
     1621 ggtggagcca agaaaagacc cgcccccagt gacgcagata taagtgagcc caaacgggtg
     1681 cgcgagtcag ttgcgcagcc atcgacgtca gacgcggaag cttcgatcaa ctacgcagac
     1741 aggtaccaaa acaaatgttc tcgtcacgtg ggcatgaatc tgatgctgtt tccctgcaga
     1801 caatgcgaga gaatgaatca gaattcaaat atctgcttca ctcacggaca gaaagactgt
     1861 ttagagtgct ttcccgtgtc agaatctcaa cccgtttctg tcgtcaaaaa ggcgtatcag
     1921 aaactgtgct acattcatca tatcatggga aaggtgccag acgcttgcac tgcctgcgat
     1981 ctggtcaatg tggatttgga tgactgcatc tttgaacaat aaatgattta aatcaggtat
     2041 ggctgccgat ggttatcttc cagattggct cgaggacaac ctctctgagg gcattcgcga
     2101 gtggtgggac ctgaaacctg gagccccgaa acccaaagcc aaccagcaaa agcaggacaa
     2161 cggccggggt ctggtgcttc ctggctacaa gtacctcgga cccttcaacg gactcgacaa
     2221 gggggagccc gtcaacgcgg cggacgcagc ggccctcgag cacgacaagg cctacgacca
     2281 gcagctcaaa gcgggtgaca atccgtacct gcggtataac cacgccgacg ccgagtttca
     2341 ggagcgtctg caagaagata cgtcatttgg gggcaacctc gggcgagcag tcttccaggc
     2401 caagaagcgg gttctcgaac ctctcggtct ggttgaggaa ggcgctaaga cggctcctgc
     2461 aaagaagaga ccggtagagc cgtcacctca gcgttccccc gactcctcca cgggcatcgg
     2521 caagaaaggc cagcagcccg ccagaaagag actcaatttc ggtcagactg gcgactcaga
     2581 gtcagtcccc gaccctcaac ctctcggaga acctccagca gcgccctcta gtgtgggatc
     2641 tggtacagtg gctgcaggcg gtggcgcacc aatggcagac aataacgaag gtgccgacgg
     2701 agtgggtaat gcctcaggaa attggcattg cgattccaca tggctgggcg acagagtcat
     2761 taccaccagc acccgaacct gggccctgcc cacctacaac aaccacctct acaagcaaat
     2821 ctccagtgaa actgcaggta gtaccaacga caacacctac ttcggctaca gcaccccctg
     2881 ggggtatttt gactttaaca gattccactg ccacttctca ccacgtgact ggcagcgact
     2941 catcaacaac aactggggat tccggcccaa gaagctgcgg ttcaagctct tcaacatcca
     3001 ggtcaaggag gtcacgacga atgacggcgt tacgaccatc gctaataacc ttaccagcac
     3061 gattcaggta ttctcggact cggaatacca gctgccgtac gtcctcggct ctgcgcacca
     3121 gggctgcctg cctccgttcc cggcggacgt cttcatgatt cctcagtacg gctacctgac
     3181 tctcaacaat ggcagtcagt ctgtgggacg ttcctccttc tactgcctgg agtacttccc
     3241 ctctcagatg ctgagaacgg gcaacaactt tgagttcagc tacagcttcg aggacgtgcc
     3301 tttccacagc agctacgcac acagccagag cctggaccgg ctgatgaatc ccctcatcga
     3361 ccagtacttg tactacctgg ccagaacaca gagtaaccca ggaggcacag ctggcaatcg
     3421 ggaactgcag ttttaccagg gcgggccttc aactatggcc gaacaagcca agaattggtt
     3481 acctggacct tgcttccggc aacaaagagt ctccaaaacg ctggatcaaa acaacaacag
     3541 caactttgct tggactggtg ccaccaaata tcacctgaac ggcagaaact cgttggttaa
     3601 tcccggcgtc gccatggcaa ctcacaagga cgacgaggac cgctttttcc catccagcgg
     3661 agtcctgatt tttggaaaaa ctggagcaac taacaaaact acattggaaa atgtgttaat
     3721 gacaaatgaa gaagaaattc gtcctactaa tcctgtagcc acggaagaat acgggatagt
     3781 cagcagcaac ttacaagcgg ctaatactgc agcccagaca caagttgtca acaaccaggg
     3841 agccttacct ggcatggtct ggcagaaccg ggacgtgtac ctgcagggtc ccatctgggc
     3901 caagattcct cacacggatg gcaactttca cccgtctcct ttgatgggcg gctttggact
     3961 taaacatccg cctcctcaga tcctgatcaa gaacactccc gttcccgcta atcctccgga
     4021 ggtgtttact cctgccaagt ttgcttcgtt catcacacag tacagcaccg gacaagtcag
     4081 cgtggaaatc gagtgggagc tgcagaagga aaacagcaag cgctggaacc cggagattca
     4141 gtacacctcc aactttgaaa agcagactgg tgtggacttt gccgttgaca gccagggtgt
     4201 ttactctgag cctcgcccta ttggcactcg ttacctcacc cgtaatctgt aattgcatgt
     4261 taatcaataa accggttgat tcgtttcagt tgaactttgg tctctgcgaa gggcgaattc
     4321 gtttaaacct gcaggactag aggtcctgta ttagaggtca cgtgagtgtt ttgcgacatt
     4381 ttgcgacacc atgtggtcac gctgggtatt taagcccgag tgagcacgca gggtctccat
     4441 tttgaagcgg gaggtttgaa cgcgcagccg ccaagccgaa ttctgcagat atccatcaca
     4501 ctggcggccg ctcgactaga gcggccgcca ccgcggtgga gctccagctt ttgttccctt
     4561 tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     4621 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     4681 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     4741 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     4801 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     4861 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     4921 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     4981 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     5041 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     5101 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     5161 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     5221 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     5281 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     5341 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     5401 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     5461 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     5521 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     5581 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     5641 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     5701 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     5761 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     5821 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     5881 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     5941 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     6001 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     6061 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     6121 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     6181 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     6241 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     6301 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     6361 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     6421 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     6481 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     6541 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     6601 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     6661 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     6721 cgcacatttc cccgaaaagt gccacctaaa ttgtaagcgt taatattttg ttaaaattcg
     6781 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
     6841 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga
     6901 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
     6961 atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag
     7021 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga
     7081 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg
     7141 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg
     7201 cgtcccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
     7261 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
     7321 cagggttttc cca
//
