LOCUS       V012816                 7371 bp    DNA     circular SYN 21-AUG-2023
DEFINITION  Exported.
ACCESSION   V012816
VERSION     V012816
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7371)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7371
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     98..114
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             complement(377..2551)
                     /codon_start=1
                     /label="capsid protein from AAV5"
                     /translation="MSFVDHPPDWLEEVGEGLREFLGLEAGPPKPKPNQQHQDQARGLV
                     LPGYNYLGPGNGLDRGEPVNRADEVAREHDISYNEQLEAGDNPYLKYNHADAEFQEKLA
                     DDTSFGGNLGKAVFQAKKRVLEPFGLVEEGAKTAPTGKRIDDHFPKRKKARTEEDSKPS
                     TSSDAEAGPSGSQQLQIPAQPASSLGADTMSAGGGGPLGDNNQGADGVGNASGDWHCDS
                     TWMGDRVVTKSTRTWVLPSYNNHQYREIKSGSVDGSNANAYFGYSTPWGYFDFNRFHSH
                     WSPRDWQRLINNYWGFRPRSLRVKIFNIQVKEVTVQDSTTTIANNLTSTVQVFTDDDYQ
                     LPYVVGNGTEGCLPAFPPQVFTLPQYGYATLNRDNTENPTERSSFFCLEYFPSKMLRTG
                     NNFEFTYNFEEVPFHSSFAPSQNLFKLANPLVDQYLYRFVSTNNTGGVQFNKNLAGRYA
                     NTYKNWFPGPMGRTQGWNLGSGVNRASVSAFATTNRMELEGASYQVPPQPNGMTNNLQG
                     SNTYALENTMIFNSQPANPGTTATYLEGNMLITSESETQPVNRVAYNVGGQMATNNQSS
                     TTAPATGTYNLQEIVPGSVWMERDVYLQGPIWAKIPETGAHFHPSPAMGGFGLKHPPPM
                     MLIKNTPVPGNITSFSDVPVSSFITQYSTGQVTVEMEWELKKENSKRWNPEIQYTNNYN
                     DPQFVDFAPDSTGEYRTTRPIGTRYLTRPL"
     CDS             complement(2571..4430)
                     /gene="Rep78"
                     /label="Protein Rep78"
                     /note="Protein Rep78 from Adeno-associated virus 2 (isolate
                     Srivastava/1982). Accession#: Q89268"
     protein_bind    complement(4593..4640)
                     /label="FRT"
                     /note="FLP-mediated recombination occurs in the 8-bp core
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     complement(4654..4670)
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     primer_bind     complement(4720..4736)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(4744..4760)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4768..4798)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4813..4834)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5122..5710)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5884..6741)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6742..6846)
                     /label="AmpR promoter"
     rep_origin      6873..7328
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta
       61 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgagcgc
      121 gccagcgctc gttcaaacct cccgctcaaa atggagaccc tgcgtgctca ctcgggctta
      181 aatacccagc gtgaccacat ggtgtcgcaa aatgtcgcaa aacactcacg tgacctctaa
      241 tacaggactc tccctaaccc tatgacgtaa ttcacgtcac gactccaccc ctccgcccgc
      301 tgtttaaacg cccgggctgt agttaatgat taacccgcca tgctacttat ctacgtagtt
      361 tattgattaa caagcattaa aggggtcggg taaggtatcg ggttccgata ggtctggtgg
      421 ttctgtattc cccggtgctg tccggggcaa agtccacaaa ctgggggtcg ttgtagttgt
      481 ttgtgtactg gatctctggg ttccacctct tggagttttc cttcttgagc tcccactcca
      541 tctccacggt gacctgcccg gtgctgtact gggtgatgaa gctgctgacg ggcacgtccg
      601 agaagctggt gatatttccg ggcacaggcg tgttcttgat gagcatcatg ggcggtgggt
      661 gtttgagtcc gaatccgccc atggccggag aggggtgaaa gtgcgccccc gtctctggga
      721 tcttggccca gatgggtcct tggaggtaca cgtccctctc catccacacg ctgccgggca
      781 cgatttcctg gaggttgtac gtgccggtcg cgggggcagt ggtggagctc tggttgttgg
      841 tggccatctg cccgccgacg ttgtacgcca cgcggttcac cggctgcgtc tcgctctcgc
      901 tggtgatgag catgttgccc tcgaggtacg tggcggtggt gcccgggttc gccggctggc
      961 tgttgaagat catagtgttc tccagggcat aggtgttgct gccctggagg ttgttggtca
     1021 tgccgttcgg ctgcgggggc acctggtaac tcgcgccctc gagctccatc ctattggtcg
     1081 tggcgaaggc gctgacactg gcgcggttga ccccggagcc caggttccag ccctgggttc
     1141 ggcccatggg ccccgggaac cagtttttgt aggtgttggc gtatctcccg gccaggttct
     1201 tgttgaactg gactccgcca gtgttatttg tgctcacgaa gcggtacaag tactggtcca
     1261 ccagcgggtt ggccagcttg aacaggttct gactgggagc gaagctggag tggaagggca
     1321 cctcctcaaa gttgtaggta aactcaaagt tgttgcccgt tctcagcatc ttgctgggaa
     1381 agtactctag gcagaagaag ctgctcctct cggtgggatt ttctgtgttg tcgcggttca
     1441 gcgtcgcgta accgtactgc ggcagcgtaa agacctgcgg agggaaggcc ggcaggcatc
     1501 cctcggtccc gttgccgacg acgtagggca gctggtagtc gtcgtccgta aacacttgga
     1561 cggtggaggt gaggttgttg gcgatggtgg tggtggagtc ctgcaccgtg acctctttga
     1621 cttgaatgtt gaagattttg actctgaggg accggggtct gaagccccag tagttgttga
     1681 tgagtctttg ccagtctcgg gggctccagt ggctgtggaa gcggttaaag tcaaagtacc
     1741 cccagggggt gctgtatcca aagtaggcgt tggcgttgct tccgtcgacg gagccgcttt
     1801 tgatctctcg gtactggtgg ttgttgtagc tgggcagcac ccaggttcgg gtggacttgg
     1861 tgacgactct gtcccccatc cacgtggaat cgcaatgcca atctcccgag gcattgccca
     1921 ctccatcggc accttggtta ttgtcgccca atgggccgcc acctcccgca gacattgtat
     1981 cagctcccaa acttgaggct ggttgggctg ggatttgcag ctgctgggat ccgctgggtc
     2041 cagcttcggc gtctgacgag gtggaaggct tggagtcctc ttcggtccga gccttctttc
     2101 tttttggaaa gtggtcgtct atccgctttc cggtaggggc cgtcttagca ccctcttcaa
     2161 ccaggccaaa aggttcgaga acccttttct tggcctgaaa gactgccttt ccgaggtttc
     2221 ccccgaagga tgtgtcgtcg gcgagcttct cctgaaactc ggcgtccgcg tggttgtact
     2281 tgaggtaggg gttgtctccc gcctcaagct gctcgttgta cgagatgtcg tgctctcgcg
     2341 cgacctcgtc tgccctgttg acaggctctc ctcgatcgag accgtttccg ggtccgagat
     2401 agttataacc aggcagcaca agaccacggg cttgatcttg atgctgctga ttgggttttg
     2461 gtttcggtgg gcccgcttca aggcccaaaa actcgcgaag accttcacca acttcttcca
     2521 accaatctgg agggtgatca acaaaagaca tacctgattt aaatcattta ttgttcaaag
     2581 atgcagtcat ccaaatccac attgaccaga tcgcaggcag tgcaagcgtc tggcaccttt
     2641 cccatgatat gatgaatgta gcacagtttc tgatacgcct ttttgacgac agaaacgggt
     2701 tgagattctg acacgggaaa gcactctaaa cagtctttct gtccgtgagt gaagcagata
     2761 tttgaattct gattcattct ctcgcattgt ctgcagggaa acagcatcag attcatgccc
     2821 acgtgacgag aacatttgtt ttggtacctg tctgcgtagt tgatcgaagc ttccgcgtct
     2881 gacgtcgatg gctgcgcaac tgactcgcgc acccgtttgg gctcacttat atctgcgtca
     2941 ctgggggcgg gtcttttctt ggctccaccc tttttgacgt agaattcatg ctccacctca
     3001 accacgtgat cctttgccca ccggaaaaag tctttgactt cctgcttggt gaccttccca
     3061 aagtcatgat ccagacggcg ggtgagttca aatttgaaca tccggtcttg caacggctgc
     3121 tggtgttcga aggtcgttga gttcccgtca atcacggcgc acatgttggt gttggaggtg
     3181 acgatcacgg gagtcgggtc tatctgggcc gaggacttgc atttctggtc cacgcgcacc
     3241 ttgcttcctc cgagaatggc tttggccgac tccacgacct tggcggtcat cttcccctcc
     3301 tcccaccaga tcaccatctt gtcgacacag tcgttgaagg gaaagttctc attggtccag
     3361 tttacgcacc cgtagaaggg cacagtgtgg gctatggcct ccgcgatgtt ggtcttcccg
     3421 gtagttgcag gcccaaacag ccagatggtg ttcctcttgc cgaacttttt cgtggcccat
     3481 cccagaaaga cggaagccgc atattgggga tcgtacccgt ttagttccaa aattttataa
     3541 atccgattgc tggaaatgtc ctccacgggc tgctggccca ccaggtagtc gggggcggtt
     3601 ttagtcaggc tcataatctt tcccgcattg tccaaggcag ccttgatttg ggaccgcgag
     3661 ttggaggccg cattgaagga gatgtatgag gcctggtcct cctggatcca ctgcttctcc
     3721 gaggtaatcc ccttgtccac gagccacccg accagctcca tgtacctggc tgaagttttt
     3781 gatctgatca ccggcgcatc agaattggga ttctgattct ctttgttctg ctcctgcgtc
     3841 tgcgacacgt gcgtcagatg ctgcgccacc aaccgtttac gctccgtgag attcaaacag
     3901 gcgcttaaat actgttccat attagtccac gcccactgga gctcaggctg ggttttgggg
     3961 agcaagtaat tggggatgta gcactcatcc accaccttgt tcccgcctcc ggcgccattt
     4021 ctggtctttg tgaccgcgaa ccagtttggc aaagtcggct cgatcccgcg gtaaattctc
     4081 tgaatcagtt tttcgcgaat ctgactcagg aaacgtccca aaaccatgga tttcaccccg
     4141 gtggtttcca cgagcacgtg catgtggaag tagctctctc ccttctcaaa ttgcacaaag
     4201 aaaagggcct ccggggcctt actcacacgg cgccattccg tcagaaagtc gcgctgcagc
     4261 ttctcggcca cggtcagggg tgcctgctca atcagattca gatccatgtc agaatctggc
     4321 ggcaactccc attccttctc ggccacccag ttcacaaagc tgtcagaaat gccgggcaga
     4381 tgctcgtcaa ggtcgctggg gaccttaatc acaatctcgt aaaaccccgg cgtggcggct
     4441 gcgcgttcaa acctcccgct tcaaaatgga gaccctgcgt gctcactcgg gcttaaatac
     4501 ccagcgtgac cacatggtgt cgcaaaatgt cgcaaaacac tcacgtgacc tctaatacag
     4561 gacctctagg ggtaccgagc tcgaatagat cagaagttcc tatactttct agagaatagg
     4621 aacttcggaa taggaacttc tgatcttccg ggggatccac tagttctaga gcggccggcg
     4681 cggggcgtta acgatatcgg cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     4741 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     4801 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     4861 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     4921 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     4981 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     5041 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     5101 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     5161 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     5221 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     5281 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     5341 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     5401 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     5461 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     5521 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     5581 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     5641 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     5701 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     5761 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     5821 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     5881 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     5941 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     6001 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     6061 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     6121 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     6181 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     6241 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     6301 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     6361 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     6421 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     6481 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     6541 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     6601 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     6661 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     6721 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     6781 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     6841 tccgcgcaca tttccccgaa aagtgccacc tgacgcgccc tgtagcggcg cattaagcgc
     6901 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     6961 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     7021 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     7081 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     7141 tttgacgttg gagtccacgt tcttaatagt ggactcttgt tccaaactgg aacaacactc
     7201 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     7261 ttaaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct
     7321 tacaatttcc attcgccatt caggctgcgc aactgttggg aagggcgatc g
//
