LOCUS       40924_3151        7278 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pAAV-hSyn-mCherry-hSyn-9(5), complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7278)
  AUTHORS   Michel U, Muller B, Koch JC, Zhang J-N.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUL-2015) Neurology, University of Goettingen, Waldweg
            33, Goettingen, Niedersachsen 37073, Germany
REFERENCE   2  (bases 1 to 7278)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 7278)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (29-JUL-2015) Neurology, University of Goettingen, Waldweg 33, 
            Goettingen, Niedersachsen 37073, Germany"
COMMENT     SGRef: number: 2; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7278
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          17..157
                     /note="beta-globin-IgG-chimeric intron"
     intron          20..153
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     promoter        complement(193..640)
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     primer_bind     complement(676..692)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    737..879
                     /label=ITR
                     /note="ITR"
     rep_origin      complement(1141..1729)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1903..2760)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2761..2865)
                     /label=AmpR promoter
     rep_origin      complement(3197..3652)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     misc_feature    3753..3896
                     /label=ITR
                     /note="ITR"
     polyA_signal    complement(3906..4113)
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    complement(4155..4743)
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     regulatory      5731..6210
                     /label=human synapsin promoter (hSyn)
                     /note="human synapsin promoter (hSyn)"
                     /regulatory_class="promoter"
     promoter        complement(5737..6184)
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     polyA_signal    complement(6250..6384)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     intron          complement(6400..6533)
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     CDS             complement(6552..7259)
                     /label=mCherry
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
ORIGIN
        1 cgaattctgc agtcgacacc tgtggagaga aaggcaaagt ggatgtcagt aagaccaata
       61 ggtgcctatc agaaacgcaa gagtcttctc tgtctcgaca agcccagttt ctatgtggtc
      121 tccttaaacc tgtcttgtaa ccttgatact tacctgcggc ccgggatcct tgctagcagc
      181 ttgaattctc gactgcgctc tcaggcacga cacgactcct ccgctgccca ccgcagactg
      241 aggcagcgct gagtcgccgg cgccgcagcg cagatggtcg cgcccgtgcc cccctatctc
      301 gcgcctcgcg tggtgcggtc cggctgggcc ggcggcggcg cggacgcgac caaggtggcc
      361 gggaagggga gtttgcgggg gaccggcgag tgacgtcagc gcgccttcag tgctgaggcg
      421 gcggtggcgc gcgccgccag gcgggggcga aggcactgtc cgcggtgctg aagctggcag
      481 tgcgcacgcg cctcgccgca tcctgtttcc cctccccctc tctgataggg gatgcgcaat
      541 ttggggaatg ggggttgggt gcttgtccag tgggtcgggg tcggtcgtca ggtaggcacc
      601 cccaccccgc ctcatcctgg tcctaaaacc cacttgcact catacgcagg gccctctgca
      661 ggcatgcaag cttatcgata ccgtcgacct cgaggggggg cccggtaccg atatcgcgtg
      721 atcctagatc taggaacccc tagtgatgga gttggccact ccctctctgc gcgctcgctc
      781 gctcactgag gccgcccggg caaagcccgg gcgtcgggcg acctttggtc gcccggcctc
      841 agtgagcgag cgagcgcgca gagagggagt ggccaacccc cccccccccc cccctgcagc
      901 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
      961 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     1021 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     1081 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     1141 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     1201 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     1261 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     1321 cgtggcgctt tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     1381 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     1441 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     1501 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     1561 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     1621 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     1681 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     1741 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     1801 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     1861 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     1921 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     1981 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     2041 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     2101 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     2161 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     2221 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     2281 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     2341 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     2401 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     2461 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     2521 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     2581 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     2641 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     2701 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     2761 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     2821 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     2881 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     2941 tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
     3001 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
     3061 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga
     3121 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag
     3181 aaaataccgc atcaggaaat tgtaaacgtt aatattttgt taaaattcgc gttaaatttt
     3241 tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca
     3301 aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta
     3361 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta
     3421 cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg
     3481 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga
     3541 aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg
     3601 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcgcgccat
     3661 tcgccattca ggctacgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
     3721 cgccagctgg ctgcaggggg gggggggggg gggttggcca ctccctctct gcgcgctcgc
     3781 tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc
     3841 tcagtgagcg agcgagcgcg cagagaggga gtggccaact ccatcactag gggttcctag
     3901 atctctcccc agcatgcctg ctattgtctt cccaatcctc ccccttgctg tcctgcccca
     3961 ccccaccccc cagaatagaa tgacacctac tcagacaatg cgatgcaatt tcctcatttt
     4021 attaggaaag gacagtggga gtggcacctt ccagggtcaa ggaaggcacg ggggaggggc
     4081 aaacaacaga tggctggcaa ctagaaggca cagtcgaggc tgatcagcga gctctagtcg
     4141 acggtatcga tcaggcgggg aggcggccca aagggagatc cgactcgtct gagggcgaag
     4201 gcgaagacgc ggaagaggcc gcagagccgg cagcaggccg cgggaaggaa ggtccgctgg
     4261 attgagggcc gaagggacgt agcagaagga cgtcccgcgc agaatccagg tggcaacaca
     4321 ggcgagcagc catggaaagg acgtcagctt ccccgacaac accacggaat tgtcagtgcc
     4381 caacagccga gcccctgtcc agcagcgggc aaggcaggcg gcgatgagtt ccgccgtggc
     4441 aatagggagg gggaaagcga aagtcccgga aaggagctga caggtggtgg caatgcccca
     4501 accagtgggg gttgcgtcag caaacacagt gcacaccacg ccacgttgcc tgacaacggg
     4561 ccacaactcc tcataaagag acagcaacca ggatttatac aaggaggaga aaatgaaagc
     4621 catacgggaa gcaatagcat gatacaaagg cattaaagca gcgtatccac atagcgtaaa
     4681 aggagcaaca tagttaagaa taccagtcaa tctttcacaa attttgtaat ccagaggttg
     4741 attatcgata agcttgcggc cgctctagtc tggtcttctg aatttgaaat agccataggg
     4801 ataaagattt ataaaaattg ccgcactgag tgtattttac ttgttgcatt ttactaacct
     4861 aggctaatta tggtgcattc attatagaat gggggccaat tatgtcaggc tctgacttgg
     4921 actgtcagag aggatggtga gctgaaaact cagtgggcct ttgcaatgga aacaggaagc
     4981 cagatggact cagctgcagc atatagaaag ggcgtaggaa ctatagtggt catttgttgt
     5041 tcccttctct gcttcctggt tgtgaataca ataaagctga cctgtcacct ctccaccctc
     5101 aagccacagg gattggtatg tgcccctcac tgggtcaatc agtgtcttcc ttggttactt
     5161 tttgccacag taggtggaaa agatggttgt tgtccacagg aattgatcgg gggatgttga
     5221 tatgaggctg ccagctgcca ttttaccacc tggagggagc ctgacagaaa actcagagaa
     5281 cccctggccc cactgatgga gcctcccaaa gaatcaggtg catccttgaa cttcccactt
     5341 gaggtaacaa gtaaatcctc aggttattat gatgtgagat tccatcattc tagaaatcag
     5401 tcatatacaa atacaactgg ctggtgtgta atgataatag tcaacactgg tgttttctgg
     5461 tggctcccgg attaaggatc cagcattccc acttctgtgg cacaggtggg tttgatccct
     5521 ggccctggaa tttttgcatg ccgtgggcat agtcaaaagg aaagaaaaat agcacttagt
     5581 gaaatgaatg ctataatgtg aggcatgctt ctaagaaatt tcatgcatga atcatttaaa
     5641 tctcacaaca gtcctatgac gtatacacca ttatcatttc tattttacag atgaagaaac
     5701 taaagcttat cgataccgtc cagcttgaat tctcgactgc gctctcaggc acgacacgac
     5761 tcctccgctg cccaccgcag actgaggcag cgctgagtcg ccggcgccgc agcgcagatg
     5821 gtcgcgcccg tgccccccta tctcgcgcct cgcgtggtgc ggtccggctg ggccggcggc
     5881 ggcgcggacg cgaccaaggt ggccgggaag gggagtttgc gggggaccgg cgagtgacgt
     5941 cagcgcgcct tcagtgctga ggcggcggtg gcgcgcgccg ccaggcgggg gcgaaggcac
     6001 tgtccgcggt gctgaagctg gcagtgcgca cgcgcctcgc cgcatcctgt ttcccctccc
     6061 cctctctgat aggggatgcg caatttgggg aatgggggtt gggtgcttgt ccagtgggtc
     6121 ggggtcggtc gtcaggtagg cacccccacc ccgcctcatc ctggtcctaa aacccacttg
     6181 cactcatacg cagggccctc tcgagtctag cgaatacgcg ttcagactag tggatcttcg
     6241 atgctagacg atccagacat gataagatac attgatgagt ttggacaaac cacaactaga
     6301 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
     6361 attataagct gcaataaaca agttgctcga agtcgacacc tgtggagaga aaggcaaagt
     6421 ggatgtcagt aagaccaata ggtgcctatc agaaacgcaa gagtcttctc tgtctcgaca
     6481 agcccagttt ctatgtggtc tccttaaacc tgtcttgtaa ccttgatact tacctgcggc
     6541 cggccgcttt acttgtacag ctcgtccatg ccgccggtgg agtggcggcc ctcggcgcgt
     6601 tcgtactgtt ccacgatggt gtagtcctcg ttgtgggagg tgatgtccaa cttgatgttg
     6661 acgttgtagg cgccgggcag ctgcacgggc ttcttggcct tgtaggtggt cttgacctca
     6721 gcgtcgtagt ggccgccgtc cttcagcttc agcctctgct tgatctcgcc cttcagggcg
     6781 ccgtcctcgg ggtacatccg ctcggaggag gcctcccagc ccatggtctt cttctgcatt
     6841 acggggccgt cggaggggaa gttggtgccg cgcagcttca ccttgtagat gaactcgccg
     6901 tcctgcaggg aggagtcctg ggtcacggtc accacgccgc cgtcctcgaa gttcatcacg
     6961 cgctcccact tgaagccctc ggggaaggac agcttcaagt agtcggggat gtcggcgggg
     7021 tgcttcacgt aggccttgga gccgtacatg aactgagggg acaggatgtc ccaggcgaag
     7081 ggcagggggc cacccttggt caccttcagc ttggcggtct gggtgccctc gtaggggcgg
     7141 ccctcgccct cgccctcgat ctcgaactcg tggccgttca cggagccctc catgtgcacc
     7201 ttgaagcgca tgaactcctt gatgatggcc atgttatcct cctcgccctt gctcaccatg
     7261 gatctcgagc tcaagctt
//
