LOCUS       62056_2435        6434 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6434)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6434
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     promoter        186..633
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     CDS             671..1597
                     /codon_start=1
                     /label=ChR2(H134R)
                     /note="H134R mutant of an N-terminal fragment of 
                     channelrhodopsin-2 from the alga Chlamydomonas reinhardtii 
                     (Nagel et al., 2005)"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVILIRLSNLTGLSNDYSRRTMGLLVSDIGTIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGAV"
     CDS             1610..2542
                     /codon_start=1
                     /label=Rluc
                     /note="luciferase from the anthozoan coelenterate Renilla 
                     reniformis (sea pansy)"
                     /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     misc_feature    2561..3149
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     polyA_signal    3181..3657
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     repeat_region   3697..3837
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      3912..4367
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4649..4753
                     /label=AmpR promoter
     CDS             4754..5611
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5785..6373
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtgtgtc tagactgcag agggccctgc
      181 gtatgagtgc aagtgggttt taggaccagg atgaggcggg gtgggggtgc ctacctgacg
      241 accgaccccg acccactgga caagcaccca acccccattc cccaaattgc gcatccccta
      301 tcagagaggg ggaggggaaa caggatgcgg cgaggcgcgt gcgcactgcc agcttcagca
      361 ccgcggacag tgccttcgcc cccgcctggc ggcgcgcgcc accgccgcct cagcactgaa
      421 ggcgcgctga cgtcactcgc cggtcccccg caaactcccc ttcccggcca ccttggtcgc
      481 gtccgcgccg ccgccggccc agccggaccg caccacgcga ggcgcgagat aggggggcac
      541 gggcgcgacc atctgcgctg cggcgccggc gactcagcgc tgcctcagtc tgcggtgggc
      601 agcggaggag tcgtgtcgtg cctgagagcg cagtcgagaa ggtaccggat cctctagagt
      661 cgacgccacc atggactatg gcggcgcttt gtctgccgtc ggacgcgaac ttttgttcgt
      721 tactaatcct gtggtggtga acgggtccgt cctggtccct gaggatcaat gttactgtgc
      781 cggatggatt gaatctcgcg gcacgaacgg cgctcagacc gcgtcaaatg tcctgcagtg
      841 gcttgcagca ggattcagca ttttgctgct gatgttctat gcctaccaaa cctggaaatc
      901 tacatgcggc tgggaggaga tctatgtgtg cgccattgaa atggttaagg tgattctcga
      961 gttctttttt gagtttaaga atccctctat gctctacctt gccacaggac accgggtgca
     1021 gtggctgcgc tatgcagagt ggctgctcac ttgtcctgtc atccttatcc gcctgagcaa
     1081 cctcaccggc ctgagcaacg actacagcag gagaaccatg ggactccttg tctcagacat
     1141 cgggactatc gtgtgggggg ctaccagcgc catggcaacc ggctatgtta aagtcatctt
     1201 cttttgtctt ggattgtgct atggcgcgaa cacatttttt cacgccgcca aagcatatat
     1261 cgagggttat catactgtgc caaagggtcg gtgccgccag gtcgtgaccg gcatggcatg
     1321 gctgtttttc gtgagctggg gtatgttccc aattctcttc attttggggc ccgaaggttt
     1381 tggcgtcctg agcgtctatg gctccaccgt aggtcacacg attattgatc tgatgagtaa
     1441 aaattgttgg gggttgttgg gacactacct gcgcgtcctg atccacgagc acatattgat
     1501 tcacggagat atccgcaaaa ccaccaaact gaacatcggc ggaacggaga tcgaggtcga
     1561 gactctcgtc gaagacgaag ccgaggccgg agccgtgcca gcggccgcca tgacttcgaa
     1621 agtttatgat ccagaacaaa ggaaacggat gataactggt ccgcagtggt gggccagatg
     1681 taaacaaatg aatgttcttg attcatttat taattattat gattcagaaa aacatgcaga
     1741 aaatgctgtt atttttttac atggtaacgc ggcctcttct tatttatggc gacatgttgt
     1801 gccacatatt gagccagtag cgcggtgtat tataccagac cttattggta tgggcaaatc
     1861 aggcaaatct ggtaatggtt cttataggtt acttgatcat tacaaatatc ttactgcatg
     1921 gtttgaactt cttaatttac caaagaagat catttttgtc ggccatgatt ggggtgcttg
     1981 tttggcattt cattatagct atgagcatca agataagatc aaagcaatag ttcacgctga
     2041 aagtgtagta gatgtgattg aatcatggga tgaatggcct gatattgaag aagatattgc
     2101 gttgatcaaa tctgaagaag gagaaaaaat ggttttggag aataacttct tcgtggaaac
     2161 catgttgcca tcaaaaatca tgagaaagtt agaaccagaa gaatttgcag catatcttga
     2221 accattcaaa gagaaaggtg aagttcgtcg tccaacatta tcatggcctc gtgaaatccc
     2281 gttagtaaaa ggtggtaaac ctgacgttgt acaaattgtt aggaattata atgcttatct
     2341 acgtgcaagt gatgatttac caaaaatgtt tattgaatcg gacccaggat tcttttccaa
     2401 tgctattgtt gaaggtgcca agaagtttcc taatactgaa tttgtcaaag taaaaggtct
     2461 tcatttttcg caagaagatg cacctgatga aatgggaaaa tatatcaaat cgttcgttga
     2521 gcgagttctc aaaaatgaac aatgaatcaa gcttatcgat aatcaacctc tggattacaa
     2581 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     2641 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     2701 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     2761 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     2821 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     2881 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     2941 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat
     3001 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     3061 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     3121 tcggatctcc ctttgggccg cctccccgca tcgataccga gcgctgctcg agagatctac
     3181 gggtggcatc cctgtgaccc ctccccagtg cctctcctgg ccctggaagt tgccactcca
     3241 gtgcccacca gccttgtcct aataaaatta agttgcatca ttttgtctga ctaggtgtcc
     3301 ttctataata ttatggggtg gaggggggtg gtatggagca aggggcaagt tgggaagaca
     3361 acctgtaggg cctgcggggt ctattgggaa ccaagctgga gtgcagtggc acaatcttgg
     3421 ctcactgcaa tctccgcctc ctgggttcaa gcgattctcc tgcctcagcc tcccgagttg
     3481 ttgggattcc aggcatgcat gaccaggctc agctaatttt tgtttttttg gtagagacgg
     3541 ggtttcacca tattggccag gctggtctcc aactcctaat ctcaggtgat ctacccacct
     3601 tggcctccca aattgctggg attacaggcg tgaaccactg ctcccttccc tgtccttctg
     3661 attttgtagg taaccacgtg cggaccgagc ggccgcagga acccctagtg atggagttgg
     3721 ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac
     3781 gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc ctgcaggggc
     3841 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa
     3901 gcaaccatag tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     3961 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     4021 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     4081 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc
     4141 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     4201 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cgggctattc
     4261 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     4321 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaattttat ggtgcactct
     4381 cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc
     4441 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
     4501 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa
     4561 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     4621 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     4681 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     4741 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     4801 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     4861 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     4921 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     4981 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     5041 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     5101 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     5161 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     5221 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     5281 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     5341 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     5401 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     5461 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     5521 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     5581 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     5641 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     5701 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     5761 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     5821 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     5881 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     5941 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     6001 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     6061 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     6121 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     6181 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     6241 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     6301 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     6361 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     6421 ttttgctcac atgt
//
