LOCUS       V014580                 6239 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014580
VERSION     V014580
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6239)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6239
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        226..673
                     /label="hSyn promoter"
                     /note="human synapsin I promoter; confers neuron-specific
                     expression (Kugler et al., 2003)"
     CDS             713..1585
                     /gene="hop"
                     /label="Halorhodopsin"
                     /note="Halorhodopsin from Natronomonas pharaonis.
                     Accession#: P15647"
     CDS             1655..2362
                     /label="mCherry"
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     misc_feature    2417..3005
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     polyA_signal    3037..3513
                     /label="hGH poly(A) signal"
                     /note="human growth hormone polyadenylation signal"
     repeat_region   3553..3693
                     /label="AAV2 ITR"
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
     rep_origin      3768..4223
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4505..4609
                     /label="AmpR promoter"
     CDS             4610..5467
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      5641..6229
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg tcctgcaggc
       61 agctgcgcgc tcgctcgctc actgaggccg cccgggcgtc gggcgacctt tggtcgcccg
      121 gcctcagtga gcgagcgagc gcgcagagag ggagtggcca actccatcac taggggttcc
      181 tgcggccgca cgcgtgtgtc tagactgcag agggccctgc gtatgagtgc aagtgggttt
      241 taggaccagg atgaggcggg gtgggggtgc ctacctgacg accgaccccg acccactgga
      301 caagcaccca acccccattc cccaaattgc gcatccccta tcagagaggg ggaggggaaa
      361 caggatgcgg cgaggcgcgt gcgcactgcc agcttcagca ccgcggacag tgccttcgcc
      421 cccgcctggc ggcgcgcgcc accgccgcct cagcactgaa ggcgcgctga cgtcactcgc
      481 cggtcccccg caaactcccc ttcccggcca ccttggtcgc gtccgcgccg ccgccggccc
      541 agccggaccg caccacgcga ggcgcgagat aggggggcac gggcgcgacc atctgcgctg
      601 cggcgccggc gactcagcgc tgcctcagtc tgcggtgggc agcggaggag tcgtgtcgtg
      661 cctgagagcg cagtcgagaa ggtaccggat ccccgggtac cggtaggcca ccatgacaga
      721 gaccctgcct cccgtgaccg agagtgccgt ggcccttcaa gccgaggtta cccaaaggga
      781 gttgttcgag ttcgtgctga acgacccttt gcttgcaagc agtctctata tcaacatcgc
      841 acttgcagga ctgagtatac tgctgttcgt ttttatgacc cgaggactcg atgatccacg
      901 ggcaaaactt attgctgtgt caaccatcct tgtgcctgtc gtcagcattg cctcctacac
      961 tggattggcg agcggcctga caatttccgt tcttgaaatg ccagcgggcc attttgcaga
     1021 aggcagctca gtgatgctgg gaggagaaga ggtagatggt gtagtcacca tgtggggacg
     1081 gtatctcacc tgggcacttt ccacgcccat gattctcctc gctctgggtc tcctggccgg
     1141 aagcaatgct acaaagctct tcacagctat cactttcgat atcgctatgt gcgtgactgg
     1201 ccttgccgcg gccctgacta cctcctccca cctcatgaga tggttctggt acgctatcag
     1261 ttgtgcatgc tttctggtgg tcttgtatat cctgctggtg gagtgggcac aggacgccaa
     1321 agccgcggga accgctgaca tgttcaatac cctgaagctg ttgacagtag tgatgtggct
     1381 ggggtatcca attgtgtggg ctcttggagt cgagggtatc gcggtgttgc ccgttggggt
     1441 gacgagctgg ggatattctt tcctggatat cgtggcaaag tacattttcg cattcttgct
     1501 cctgaactat ctgacgtcaa acgaatctgt cgtgtccggc agcattttgg atgttccatc
     1561 tgcttctggg accccggctg atgatgcggc cgccaagagc aggatcacca gcgagggcga
     1621 gtacatcccc ctggaccaga tcgacatcaa cgtgatggtg agcaagggcg aggaggataa
     1681 catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg
     1741 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc
     1801 caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca
     1861 gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa
     1921 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt
     1981 ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct
     2041 gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga
     2101 ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag
     2161 gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa
     2221 gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca
     2281 caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg
     2341 cggcatggac gagctgtaca agtagttctg ctacgagaac gaggtgtaat gagaattcga
     2401 tatcaagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     2461 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     2521 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     2581 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     2641 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     2701 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     2761 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc
     2821 ctttccttgg ctgctcgcct atgttgccac ctggattctg cgcgggacgt ccttctgcta
     2881 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     2941 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     3001 cccgcatcga taccgagcgc tgctcgagag atctacgggt ggcatccctg tgacccctcc
     3061 ccagtgcctc tcctggccct ggaagttgcc actccagtgc ccaccagcct tgtcctaata
     3121 aaattaagtt gcatcatttt gtctgactag gtgtccttct ataatattat ggggtggagg
     3181 ggggtggtat ggagcaaggg gcaagttggg aagacaacct gtagggcctg cggggtctat
     3241 tgggaaccaa gctggagtgc agtggcacaa tcttggctca ctgcaatctc cgcctcctgg
     3301 gttcaagcga ttctcctgcc tcagcctccc gagttgttgg gattccaggc atgcatgacc
     3361 aggctcagct aatttttgtt tttttggtag agacggggtt tcaccatatt ggccaggctg
     3421 gtctccaact cctaatctca ggtgatctac ccaccttggc ctcccaaatt gctgggatta
     3481 caggcgtgaa ccactgctcc cttccctgtc cttctgattt tgtaggtaac cacgtgcgga
     3541 ccgagcggcc gcaggaaccc ctagtgatgg agttggccac tccctctctg cgcgctcgct
     3601 cgctcactga ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct
     3661 cagtgagcga gcgagcgcgc agctgcctgc aggggcgcct gatgcggtat tttctcctta
     3721 cgcatctgtg cggtatttca caccgcatac gtcaaagcaa ccatagtacg cgccctgtag
     3781 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     3841 cgccttagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     3901 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     3961 cctcgacccc aaaaaacttg atttgggtga tggttcacgt agtgggccat cgccctgata
     4021 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     4081 aactggaaca acactcaact ctatctcggg ctattctttt gatttataag ggattttgcc
     4141 gatttcggtc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     4201 caaaatatta acgtttacaa ttttatggtg cactctcagt acaatctgct ctgatgccgc
     4261 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     4321 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     4381 gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt
     4441 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     4501 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     4561 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     4621 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     4681 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     4741 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     4801 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     4861 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     4921 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     4981 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     5041 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     5101 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     5161 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     5221 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     5281 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     5341 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     5401 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     5461 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     5521 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     5581 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     5641 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     5701 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     5761 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     5821 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     5881 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     5941 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     6001 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     6061 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     6121 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     6181 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagca
//
