LOCUS       V014578                 7178 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014578
VERSION     V014578
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7178)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7178
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        226..673
                     /label="hSyn promoter"
                     /note="human synapsin I promoter; confers neuron-specific
                     expression (Kugler et al., 2003)"
     CDS             713..1585
                     /gene="hop"
                     /label="Halorhodopsin"
                     /note="Halorhodopsin from Natronomonas pharaonis.
                     Accession#: P15647"
     CDS             1655..3304
                     /label="luciferase"
                     /note="firefly luciferase"
     misc_feature    3356..3944
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     polyA_signal    3976..4452
                     /label="hGH poly(A) signal"
                     /note="human growth hormone polyadenylation signal"
     repeat_region   4492..4632
                     /label="AAV2 ITR"
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
     rep_origin      4707..5162
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5444..5548
                     /label="AmpR promoter"
     CDS             5549..6406
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      6580..7168
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg tcctgcaggc
       61 agctgcgcgc tcgctcgctc actgaggccg cccgggcgtc gggcgacctt tggtcgcccg
      121 gcctcagtga gcgagcgagc gcgcagagag ggagtggcca actccatcac taggggttcc
      181 tgcggccgca cgcgtgtgtc tagactgcag agggccctgc gtatgagtgc aagtgggttt
      241 taggaccagg atgaggcggg gtgggggtgc ctacctgacg accgaccccg acccactgga
      301 caagcaccca acccccattc cccaaattgc gcatccccta tcagagaggg ggaggggaaa
      361 caggatgcgg cgaggcgcgt gcgcactgcc agcttcagca ccgcggacag tgccttcgcc
      421 cccgcctggc ggcgcgcgcc accgccgcct cagcactgaa ggcgcgctga cgtcactcgc
      481 cggtcccccg caaactcccc ttcccggcca ccttggtcgc gtccgcgccg ccgccggccc
      541 agccggaccg caccacgcga ggcgcgagat aggggggcac gggcgcgacc atctgcgctg
      601 cggcgccggc gactcagcgc tgcctcagtc tgcggtgggc agcggaggag tcgtgtcgtg
      661 cctgagagcg cagtcgagaa ggtaccggat ccccgggtac cggtaggcca ccatgacaga
      721 gaccctgcct cccgtgaccg agagtgccgt ggcccttcaa gccgaggtta cccaaaggga
      781 gttgttcgag ttcgtgctga acgacccttt gcttgcaagc agtctctata tcaacatcgc
      841 acttgcagga ctgagtatac tgctgttcgt ttttatgacc cgaggactcg atgatccacg
      901 ggcaaaactt attgctgtgt caaccatcct tgtgcctgtc gtcagcattg cctcctacac
      961 tggattggcg agcggcctga caatttccgt tcttgaaatg ccagcgggcc attttgcaga
     1021 aggcagctca gtgatgctgg gaggagaaga ggtagatggt gtagtcacca tgtggggacg
     1081 gtatctcacc tgggcacttt ccacgcccat gattctcctc gctctgggtc tcctggccgg
     1141 aagcaatgct acaaagctct tcacagctat cactttcgat atcgctatgt gcgtgactgg
     1201 ccttgccgcg gccctgacta cctcctccca cctcatgaga tggttctggt acgctatcag
     1261 ttgtgcatgc tttctggtgg tcttgtatat cctgctggtg gagtgggcac aggacgccaa
     1321 agccgcggga accgctgaca tgttcaatac cctgaagctg ttgacagtag tgatgtggct
     1381 ggggtatcca attgtgtggg ctcttggagt cgagggtatc gcggtgttgc ccgttggggt
     1441 gacgagctgg ggatattctt tcctggatat cgtggcaaag tacattttcg cattcttgct
     1501 cctgaactat ctgacgtcaa acgaatctgt cgtgtccggc agcattttgg atgttccatc
     1561 tgcttctggg accccggctg atgatgcggc cgccaagagc aggatcacca gcgagggcga
     1621 gtacatcccc ctggaccaga tcgacatcaa cgtgatggaa gacgccaaaa acataaagaa
     1681 aggcccggcg ccattctatc ctcttgagga tggaaccgct ggagagcaac tgcataaggc
     1741 tatgaagaga tacgccctgg ttcctggaac aattgctttt acagatgcac atatcgaggt
     1801 gaacatcacg tacgcggaat acttcgaaat gtccgttcgg ttggcagaag ctatgaaacg
     1861 atatgggctg aatacaaatc acagaatcgt cgtatgcagt gaaaactctc ttcaattctt
     1921 tatgccggtg ttgggcgcgt tatttatcgg agttgcagtt gcgcccgcga acgacattta
     1981 taatgaacgt gaattgctca acagtatgaa catttcgcag cctaccgtag tgtttgtttc
     2041 caaaaagggg ttgcaaaaaa ttttgaacgt gcaaaaaaaa ttaccaataa tccagaaaat
     2101 tattatcatg gattctaaaa cggattacca gggatttcag tcgatgtaca cgttcgtcac
     2161 atctcatcta cctcccggtt ttaatgaata cgattttgta ccagagtcct ttgatcgtga
     2221 caaaacaatt gcactgataa tgaactcctc tggatctact gggttaccta agggtgtggc
     2281 ccttccgcat agaactgcct gcgtcagatt ctcgcatgcc agagatccta tttttggcaa
     2341 tcaaatcatt ccggatactg cgattttaag tgttgttcca ttccatcacg gttttggaat
     2401 gtttactaca ctcggatatt tgatatgtgg atttcgagtc gtcttaatgt atagatttga
     2461 agaagagctg tttttacgat cccttcagga ttacaaaatt caaagtgcgt tgctagtacc
     2521 aaccctattt tcattcttcg ccaaaagcac tctgattgac aaatacgatt tatctaattt
     2581 acacgaaatt gcttctgggg gcgcacctct ttcgaaagaa gtcggggaag cggttgcaaa
     2641 acgcttccat cttccaggga tacgacaagg atatgggctc actgagacta catcagctat
     2701 tctgattaca cccgaggggg atgataaacc gggcgcggtc ggtaaagttg ttccattttt
     2761 tgaagcgaag gttgtggatc tggataccgg gaaaacgctg ggcgttaatc agagaggcga
     2821 attatgtgtc agaggaccta tgattatgtc cggttatgta aacaatccgg aagcgaccaa
     2881 cgccttgatt gacaaggatg gatggctaca ttctggagac atagcttact gggacgaaga
     2941 cgaacacttc ttcatagttg accgcttgaa gtctttaatt aaatacaaag gataccaggt
     3001 ggcccccgct gaattggagt cgatattgtt acaacacccc aacatcttcg acgcgggcgt
     3061 ggcaggtctt cccgacgatg acgccggtga acttcccgcc gccgttgttg ttttggagca
     3121 cggaaagacg atgacggaaa aagagatcgt ggattacgtc gccagtcaag taacaaccgc
     3181 gaaaaagttg cgcggaggag ttgtgtttgt ggacgaagta ccgaaaggtc ttaccggaaa
     3241 actcgacgca agaaaaatca gagagatcct cataaaggcc aagaagggcg gaaagtccaa
     3301 attgttctgc tacgagaacg aggtgtaatg agaattcgat atcaagctta tcgataatca
     3361 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     3421 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     3481 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     3541 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     3601 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     3661 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     3721 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgccta
     3781 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     3841 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     3901 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat accgagcgct
     3961 gctcgagaga tctacgggtg gcatccctgt gacccctccc cagtgcctct cctggccctg
     4021 gaagttgcca ctccagtgcc caccagcctt gtcctaataa aattaagttg catcattttg
     4081 tctgactagg tgtccttcta taatattatg gggtggaggg gggtggtatg gagcaagggg
     4141 caagttggga agacaacctg tagggcctgc ggggtctatt gggaaccaag ctggagtgca
     4201 gtggcacaat cttggctcac tgcaatctcc gcctcctggg ttcaagcgat tctcctgcct
     4261 cagcctcccg agttgttggg attccaggca tgcatgacca ggctcagcta atttttgttt
     4321 ttttggtaga gacggggttt caccatattg gccaggctgg tctccaactc ctaatctcag
     4381 gtgatctacc caccttggcc tcccaaattg ctgggattac aggcgtgaac cactgctccc
     4441 ttccctgtcc ttctgatttt gtaggtaacc acgtgcggac cgagcggccg caggaacccc
     4501 tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag gccgggcgac
     4561 caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag cgagcgcgca
     4621 gctgcctgca ggggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     4681 accgcatacg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa gcgcggcggg
     4741 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccttagcgc ccgctccttt
     4801 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     4861 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     4921 tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     4981 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaactc
     5041 tatctcgggc tattcttttg atttataagg gattttgccg atttcggtct attggttaaa
     5101 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat
     5161 tttatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
     5221 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
     5281 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
     5341 acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     5401 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     5461 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     5521 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     5581 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     5641 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     5701 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     5761 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     5821 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     5881 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     5941 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     6001 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     6061 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     6121 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     6181 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     6241 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     6301 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     6361 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     6421 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     6481 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     6541 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     6601 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     6661 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     6721 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     6781 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     6841 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     6901 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     6961 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     7021 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     7081 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     7141 ctcgtcaggg gggcggagcc tatggaaaaa cgccagca
//
