LOCUS       62056_2565        5689 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5689)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5689
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     enhancer        221..524
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        525..728
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          877..1255
                     /label=chimeric intron
                     /note="fusion between human cytomegalovirus intron A and
                     human beta-globin intron 2"
     CDS             1337..2029
                     /codon_start=1
                     /label=ZsGreen1
                     /note="Zoanthus green fluorescent protein"
                     /translation="MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFKGKQAIN
                     LCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDGAV
                     CICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGILKG
                     DVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWHLTEH
                     AIASGSALP"
     polyA_signal    2041..2517
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     promoter        2639..2879
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
     repeat_region   2952..3092
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      3167..3622
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3904..4008
                     /label=AmpR promoter
     CDS             4009..4866
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5040..5628
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtggagc tagttattaa tagtaatcaa
      181 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
      241 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
      301 ttcccatagt aacgtcaata gggactttcc attgacgtca atgggtggag tatttacggt
      361 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
      421 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
      481 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
      541 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
      601 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
      661 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
      721 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
      781 tccatagaag acaccgggac cgatccagcc tccgcggatt cgaatcccgg ccgggaacgg
      841 tgcattggaa cgcggattcc ccgtgccaag agtgacgtaa gtaccgccta tagagtctat
      901 aggcccacaa aaaatgcttt cttcttttaa tatacttttt tgtttatctt atttctaata
      961 ctttccctaa tctctttctt tcagggcaat aatgatacaa tgtatcatgc ctctttgcac
     1021 cattctaaag aataacagtg ataatttctg ggttaaggca atagcaatat ttctgcatat
     1081 aaatatttct gcatataaat tgtaactgat gtaagaggtt tcatattgct aatagcagct
     1141 acaatccagc taccattctg cttttatttt atggttggga taaggctgga ttattctgag
     1201 tccaagctag gcccttttgc taatcatgtt catacctctt atcttcctcc cacagctcct
     1261 gggcaacgtg ctggtctgtg tgctggccca tcactttggc aaagaattgg gattcgaaca
     1321 tcgattgaat tccaccatgg cccagtccaa gcacggcctg accaaggaga tgaccatgaa
     1381 gtaccgcatg gagggctgcg tggacggcca caagttcgtg atcaccggcg agggcatcgg
     1441 ctaccccttc aagggcaagc aggccatcaa cctgtgcgtg gtggagggcg gccccttgcc
     1501 cttcgccgag gacatcttgt ccgccgcctt catgtacggc aaccgcgtgt tcaccgagta
     1561 cccccaggac atcgtcgact acttcaagaa ctcctgcccc gccggctaca cctgggaccg
     1621 ctccttcctg ttcgaggacg gcgccgtgtg catctgcaac gccgacatca ccgtgagcgt
     1681 ggaggagaac tgcatgtacc acgagtccaa gttctacggc gtgaacttcc ccgccgacgg
     1741 ccccgtgatg aagaagatga ccgacaactg ggagccctcc tgcgagaaga tcatccccgt
     1801 gcccaagcag ggcatcttga agggcgacgt gagcatgtac ctgctgctga aggacggtgg
     1861 ccgcttgcgc tgccagttcg acaccgtgta caaggccaag tccgtgcccc gcaagatgcc
     1921 cgactggcac ttcatccagc acaagctgac ccgcgaggac cgcagcgacg ccaagaacca
     1981 gaagtggcac ctgaccgagc acgccatcgc ctccggctcc gccttgccct aaagatctac
     2041 gggtggcatc cctgtgaccc ctccccagtg cctctcctgg ccctggaagt tgccactcca
     2101 gtgcccacca gccttgtcct aataaaatta agttgcatca ttttgtctga ctaggtgtcc
     2161 ttctataata ttatggggtg gaggggggtg gtatggagca aggggcaagt tgggaagaca
     2221 acctgtaggg cctgcggggt ctattgggaa ccaagctgga gtgcagtggc acaatcttgg
     2281 ctcactgcaa tctccgcctc ctgggttcaa gcgattctcc tgcctcagcc tcccgagttg
     2341 ttgggattcc aggcatgcat gaccaggctc agctaatttt tgtttttttg gtagagacgg
     2401 ggtttcacca tattggccag gctggtctcc aactcctaat ctcaggtgat ctacccacct
     2461 tggcctccca aattgctggg attacaggcg tgaaccactg ctcccttccc tgtccttctg
     2521 attttgtagg taaccacgtg cggacctcga aattccccag tggaaagacg cgcaggcaaa
     2581 acgcaccacg tgacggagcg tgaccgcgcg ccgagcgcgc gccaaggtcg ggcaggaaga
     2641 gggcctattt cccatgattc cttcatattt gcatatacga tacaaggctg ttagagagat
     2701 aattagaatt aatttgactg taaacacaaa gatattagta caaaatacgt gacgtagaaa
     2761 gtaataattt cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg actatcatat
     2821 gcttaccgta acttgaaagt atttcgattt cttgggttta tatatcttgt ggaaaggacg
     2881 cgggatccgc gagctgcagg actctaactc gagttagagt cctgcagctc gctttttaag
     2941 cttgcggccg caggaacccc tagtgatgga gttggccact ccctctctgc gcgctcgctc
     3001 gctcactgag gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc
     3061 agtgagcgag cgagcgcgca gctgcctgca ggggcgcctg atgcggtatt ttctccttac
     3121 gcatctgtgc ggtatttcac accgcatacg tcaaagcaac catagtacgc gccctgtagc
     3181 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     3241 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     3301 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     3361 ctcgacccca aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag
     3421 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     3481 actggaacaa cactcaaccc tatctcgggc tattcttttg atttataagg gattttgccg
     3541 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     3601 aaaatattaa cgtttacaat tttatggtgc actctcagta caatctgctc tgatgccgca
     3661 tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
     3721 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
     3781 ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta
     3841 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     3901 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     3961 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     4021 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     4081 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     4141 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     4201 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     4261 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     4321 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     4381 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     4441 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     4501 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     4561 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     4621 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     4681 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     4741 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     4801 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     4861 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     4921 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     4981 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     5041 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     5101 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     5161 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     5221 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     5281 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     5341 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     5401 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     5461 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     5521 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     5581 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     5641 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgt
//
