LOCUS       62056_2545        5743 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5743)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5743
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     enhancer        221..524
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        525..728
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          877..1255
                     /label=chimeric intron
                     /note="fusion between human cytomegalovirus intron A and
                     human beta-globin intron 2"
     CDS             1337..2029
                     /codon_start=1
                     /label=ZsGreen1
                     /note="Zoanthus green fluorescent protein"
                     /translation="MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFKGKQAIN
                     LCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDGAV
                     CICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGILKG
                     DVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWHLTEH
                     AIASGSALP"
     polyA_signal    2041..2517
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     promoter        2639..2879
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
     repeat_region   3006..3146
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      3221..3676
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3958..4062
                     /label=AmpR promoter
     CDS             4063..4920
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5094..5682
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtggagc tagttattaa tagtaatcaa
      181 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
      241 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
      301 ttcccatagt aacgtcaata gggactttcc attgacgtca atgggtggag tatttacggt
      361 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
      421 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
      481 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
      541 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
      601 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
      661 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
      721 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
      781 tccatagaag acaccgggac cgatccagcc tccgcggatt cgaatcccgg ccgggaacgg
      841 tgcattggaa cgcggattcc ccgtgccaag agtgacgtaa gtaccgccta tagagtctat
      901 aggcccacaa aaaatgcttt cttcttttaa tatacttttt tgtttatctt atttctaata
      961 ctttccctaa tctctttctt tcagggcaat aatgatacaa tgtatcatgc ctctttgcac
     1021 cattctaaag aataacagtg ataatttctg ggttaaggca atagcaatat ttctgcatat
     1081 aaatatttct gcatataaat tgtaactgat gtaagaggtt tcatattgct aatagcagct
     1141 acaatccagc taccattctg cttttatttt atggttggga taaggctgga ttattctgag
     1201 tccaagctag gcccttttgc taatcatgtt catacctctt atcttcctcc cacagctcct
     1261 gggcaacgtg ctggtctgtg tgctggccca tcactttggc aaagaattgg gattcgaaca
     1321 tcgattgaat tccaccatgg cccagtccaa gcacggcctg accaaggaga tgaccatgaa
     1381 gtaccgcatg gagggctgcg tggacggcca caagttcgtg atcaccggcg agggcatcgg
     1441 ctaccccttc aagggcaagc aggccatcaa cctgtgcgtg gtggagggcg gccccttgcc
     1501 cttcgccgag gacatcttgt ccgccgcctt catgtacggc aaccgcgtgt tcaccgagta
     1561 cccccaggac atcgtcgact acttcaagaa ctcctgcccc gccggctaca cctgggaccg
     1621 ctccttcctg ttcgaggacg gcgccgtgtg catctgcaac gccgacatca ccgtgagcgt
     1681 ggaggagaac tgcatgtacc acgagtccaa gttctacggc gtgaacttcc ccgccgacgg
     1741 ccccgtgatg aagaagatga ccgacaactg ggagccctcc tgcgagaaga tcatccccgt
     1801 gcccaagcag ggcatcttga agggcgacgt gagcatgtac ctgctgctga aggacggtgg
     1861 ccgcttgcgc tgccagttcg acaccgtgta caaggccaag tccgtgcccc gcaagatgcc
     1921 cgactggcac ttcatccagc acaagctgac ccgcgaggac cgcagcgacg ccaagaacca
     1981 gaagtggcac ctgaccgagc acgccatcgc ctccggctcc gccttgccct aaagatctac
     2041 gggtggcatc cctgtgaccc ctccccagtg cctctcctgg ccctggaagt tgccactcca
     2101 gtgcccacca gccttgtcct aataaaatta agttgcatca ttttgtctga ctaggtgtcc
     2161 ttctataata ttatggggtg gaggggggtg gtatggagca aggggcaagt tgggaagaca
     2221 acctgtaggg cctgcggggt ctattgggaa ccaagctgga gtgcagtggc acaatcttgg
     2281 ctcactgcaa tctccgcctc ctgggttcaa gcgattctcc tgcctcagcc tcccgagttg
     2341 ttgggattcc aggcatgcat gaccaggctc agctaatttt tgtttttttg gtagagacgg
     2401 ggtttcacca tattggccag gctggtctcc aactcctaat ctcaggtgat ctacccacct
     2461 tggcctccca aattgctggg attacaggcg tgaaccactg ctcccttccc tgtccttctg
     2521 attttgtagg taaccacgtg cggacctcga aattccccag tggaaagacg cgcaggcaaa
     2581 acgcaccacg tgacggagcg tgaccgcgcg ccgagcgcgc gccaaggtcg ggcaggaaga
     2641 gggcctattt cccatgattc cttcatattt gcatatacga tacaaggctg ttagagagat
     2701 aattagaatt aatttgactg taaacacaaa gatattagta caaaatacgt gacgtagaaa
     2761 gtaataattt cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg actatcatat
     2821 gcttaccgta acttgaaagt atttcgattt cttgggttta tatatcttgt ggaaaggacg
     2881 cgggatccct aagacttcct cgagacctta ggcaaagctt tgcgagttgc acacgtgtca
     2941 aggctgccag ctaggttaat ctcaattctt ggaagttaga gctcgctttt taagcttgcg
     3001 gccgcaggaa cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac
     3061 tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag
     3121 cgagcgagcg cgcagctgcc tgcaggggcg cctgatgcgg tattttctcc ttacgcatct
     3181 gtgcggtatt tcacaccgca tacgtcaaag caaccatagt acgcgccctg tagcggcgca
     3241 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     3301 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     3361 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     3421 cccaaaaaac ttgatttggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     3481 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     3541 acaacactca accctatctc gggctattct tttgatttat aagggatttt gccgatttcg
     3601 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     3661 ttaacgttta caattttatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     3721 agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
     3781 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca
     3841 ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt
     3901 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
     3961 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     4021 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     4081 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     4141 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     4201 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     4261 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     4321 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     4381 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     4441 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     4501 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     4561 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     4621 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     4681 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     4741 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     4801 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     4861 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     4921 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     4981 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     5041 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     5101 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     5161 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     5221 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     5281 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     5341 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     5401 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     5461 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     5521 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     5581 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     5641 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     5701 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgt
//
