LOCUS       62056_2505        5691 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5691)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5691
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     enhancer        221..524
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        525..727
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          876..1254
                     /label=chimeric intron
                     /note="fusion between human cytomegalovirus intron A and
                     human beta-globin intron 2"
     CDS             1325..2056
                     /codon_start=1
                     /label=TagRFP-T
                     /note="photostable variant of TagRFP (Shaner et al., 2008)"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
     polyA_signal    2438..2914
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     repeat_region   2954..3094
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      3169..3624
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3906..4010
                     /label=AmpR promoter
     CDS             4011..4868
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5042..5630
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtggagc tagttattaa tagtaatcaa
      181 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
      241 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
      301 ttcccatagt aacgtcaata gggactttcc attgacgtca atgggtggag tatttacggt
      361 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
      421 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
      481 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
      541 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
      601 ttgacgtcaa tgggagtttg ttttgcacca aaatcaacgg gactttccaa aatgtcgtaa
      661 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
      721 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
      781 ccatagaaga caccgggacc gatccagcct ccgcggattc gaatcccggc cgggaacggt
      841 gcattggaac gcggattccc cgtgccaaga gtgacgtaag taccgcctat agagtctata
      901 ggcccacaaa aaatgctttc ttcttttaat atactttttt gtttatctta tttctaatac
      961 tttccctaat ctctttcttt cagggcaata atgatacaat gtatcatgcc tctttgcacc
     1021 attctaaaga ataacagtga taatttctgg gttaaggcaa tagcaatatt tctgcatata
     1081 aatatttctg catataaatt gtaactgatg taagaggttt catattgcta atagcagcta
     1141 caatccagct accattctgc ttttatttta tggttgggat aaggctggat tattctgagt
     1201 ccaagctagg cccttttgct aatcatgttc atacctctta tcttcctccc acagctcctg
     1261 ggcaacgtgc tggtctgtgt gctggcccat cactttggca aagaattggg attcgaacat
     1321 cgatatggtg tctaagggcg aagagctgat taaggagaac atgcacatga agctgtacat
     1381 ggagggcacc gtgaacaacc accacttcaa gtgcacatcc gagggcgaag gcaagcccta
     1441 cgagggcacc cagaccatga gaatcaaggt ggtcgagggc ggccctctcc ccttcgcctt
     1501 cgacatcctg gctaccagct tcatgtacgg cagcagaacc ttcatcaacc acacccaggg
     1561 catccccgat ttctttaagc agtccttccc tgagggcttc acatgggaga gagtcaccac
     1621 atacgaagac gggggcgtgc tgaccgctac ccaggacacc agcctccagg acggctgcct
     1681 catctacaac gtcaagatca gaggggtgaa cttcccatcc aacggccctg tgatgcagaa
     1741 gaaaacactc ggctgggagg ccaacaccga gatgctgtac cccgctgacg gcggcctgga
     1801 aggcagaacc gacatggccc tgaagctcgt gggcgggggc cacctgatct gcaacttcaa
     1861 gaccacatac agatccaaga aacccgctaa gaacctcaag atgcccggcg tctactatgt
     1921 ggaccacaga ctggaaagaa tcaaggaggc cgacaaagag acctacgtcg agcagcacga
     1981 ggtggctgtg gccagatact gcgacctccc tagcaaactg gggcacaaac ttaatggcat
     2041 ggacgagctg tacaagagtg gcggctccgg cggttctaga acggcaagca cggaaaactt
     2101 taaaaatgtt aaagaaaaat atcagcaaat gcgaggtgat gctttaaaaa cagaaatcct
     2161 ggctgatttc aaggataaac tggctgaagc taccgatgaa cagagcctta agcaaattgt
     2221 tgctgaattg aaaagtaaag atgaatatag aattttggct aaggggcaag ggttaacaac
     2281 ccagctttta gggttaaaga caagttcagt gtcttcattt gagaaaatgg ttgaggaaac
     2341 aagagaaagt attaagtctc aagaaagaca aaccattaag ataaaataag tcgacctgca
     2401 gaagcttgcc tcgagcagcg ctgctcgaga gatctacggg tggcatccct gtgacccctc
     2461 cccagtgcct ctcctggccc tggaagttgc cactccagtg cccaccagcc ttgtcctaat
     2521 aaaattaagt tgcatcattt tgtctgacta ggtgtccttc tataatatta tggggtggag
     2581 gggggtggta tggagcaagg ggcaagttgg gaagacaacc tgtagggcct gcggggtcta
     2641 ttgggaacca agctggagtg cagtggcaca atcttggctc actgcaatct ccgcctcctg
     2701 ggttcaagcg attctcctgc ctcagcctcc cgagttgttg ggattccagg catgcatgac
     2761 caggctcagc taatttttgt ttttttggta gagacggggt ttcaccatat tggccaggct
     2821 ggtctccaac tcctaatctc aggtgatcta cccaccttgg cctcccaaat tgctgggatt
     2881 acaggcgtga accactgctc ccttccctgt ccttctgatt ttgtaggtaa ccacgtgcgg
     2941 accgagcggc cgcaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc
     3001 tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc
     3061 tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc tgatgcggta ttttctcctt
     3121 acgcatctgt gcggtatttc acaccgcata cgtcaaagca accatagtac gcgccctgta
     3181 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     3241 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     3301 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     3361 acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat
     3421 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     3481 aaactggaac aacactcaac cctatctcgg gctattcttt tgatttataa gggattttgc
     3541 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     3601 acaaaatatt aacgtttaca attttatggt gcactctcag tacaatctgc tctgatgccg
     3661 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     3721 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     3781 ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt
     3841 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
     3901 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     3961 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     4021 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     4081 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     4141 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     4201 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     4261 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     4321 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     4381 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     4441 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     4501 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     4561 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     4621 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     4681 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     4741 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     4801 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     4861 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     4921 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     4981 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     5041 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     5101 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     5161 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     5221 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     5281 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     5341 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     5401 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     5461 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     5521 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     5581 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     5641 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg t
//
