LOCUS       62056_2530        5034 bp DNA     circular SYN 30-NOV-2021
DEFINITION  Cloning vector pAAV-TRE-NS1NF, complete sequence.
ACCESSION   MZ708022
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5034)
  AUTHORS   Li E, Guo J, Oh SJ, Luo Y, Oliveros HC, Du W, Arano R, Kim Y, Chen 
            YT, Eitson J, Lin DT, Li Y, Roberts T, Schoggins JW, Xu W.
  TITLE     Anterograde transneuronal tracing and genetic control with 
            engineered yellow fever vaccine YFV-17D
  JOURNAL   Nat Methods (2021) In press
  PUBMED    34824475
REFERENCE   2  (bases 1 to 5034)
  AUTHORS   Xu W.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2021) Neuroscience, UT Southwestern Medical 
            Center, Dallas, TX 75229, USA
REFERENCE   3  (bases 1 to 5034)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Methods
            (2021) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (03-AUG-2021) Neuroscience, UT Southwestern Medical Center, Dallas, 
            TX 75229, USA"
FEATURES             Location/Qualifiers
     source          1..5034
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     polyA_signal    complement(164..245)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        261..575
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     CDS             612..1745
                     /codon_start=1
                     /transl_table=11
                     /product="NS1"
                     /label=NS1
                     /protein_id="UBZ25899.1"
                     /translation="MNMTMSMSMILVGVIMMFLSLGVGADQGCAINFGKRELKCGDGIF
                     IFRDSDDWLNKYSYYPEDPVKLASIVKASFEEGKCGLNSVDSLEHEMWRSRADEINAIF
                     EENEVDISVVVQDPKNVYQRGTHPFSRIRDGLQYGWKTWGKNLVFSPGRKNGSFIIDGK
                     SRKECPFSNRVWNSFQIEEFGTGVFTTRVYMDAVFEYTIDCDGSILGAAVNGKKSAHGS
                     PTFWMGSHEVNGTWMIHTLEALDYKECEWPLTHTIGTSVEESEMFMPRSIGGPVSSHNH
                     IPGYKVQTNGPWMQVPLEVKREACPGTSVIIDGNCDGRGKSTRSTTDSGKVIPEWCCRS
                     CTMPPVSFHGSDGCWYPMEIRPRKTHESHLVRSWVTA"
     polyA_signal    1781..2257
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     repeat_region   2297..2437
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      2512..2967
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3249..3353
                     /label=AmpR promoter
     CDS             3354..4211
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      4385..4973
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtatttg tcggcagtga aaaaaatgct
      181 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
      241 aagttaacaa caacaatttc gagtttactc cctatcagtg atagagaacg tatgtcgagt
      301 ttactcccta tcagtgatag agaacgatgt cgagtttact ccctatcagt gatagagaac
      361 gtatgtcgag tttactccct atcagtgata gagaacgtat gtcgagttta ctccctatca
      421 gtgatagaga acgtatgtcg agtttatccc tatcagtgat agagaacgta tgtcgagttt
      481 actccctatc agtgatagag aacgtatgtc gaggtaggcg tgtacggtgg gaggcctata
      541 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag atttcgagct cggtaccaat
      601 aatcagccac catgaacatg acaatgtcca tgagcatgat cttggtagga gtgatcatga
      661 tgtttttgtc tctaggagtt ggggcggatc aaggatgcgc catcaacttt ggcaagagag
      721 agctcaagtg cggagatggt atcttcatat ttagagactc tgatgactgg ctgaacaagt
      781 actcatacta tccagaagat cctgtgaagc ttgcatcaat agtgaaagcc tcttttgaag
      841 aagggaagtg tggcctaaat tcagttgact cccttgagca tgagatgtgg agaagcaggg
      901 cagatgagat caatgccatt tttgaggaaa acgaggtgga catttctgtt gtcgtgcagg
      961 atccaaagaa tgtttaccag agaggaactc atccattttc cagaattcgg gatggtctgc
     1021 agtatggttg gaagacttgg ggtaagaacc ttgtgttctc cccagggagg aagaatggaa
     1081 gcttcatcat agatggaaag tccaggaaag aatgcccgtt ttcaaaccgg gtctggaatt
     1141 ctttccagat agaggagttt gggacgggag tgttcaccac acgcgtgtac atggacgcag
     1201 tctttgaata caccatagac tgcgatggat ctatcttggg tgcagcggtg aacggaaaaa
     1261 agagtgccca tggctctcca acattttgga tgggaagtca tgaagtaaat gggacatgga
     1321 tgatccacac cttggaggca ttagattaca aggagtgtga gtggccactg acacatacga
     1381 ttggaacatc agttgaagag agtgaaatgt tcatgccgag atcaatcgga ggcccagtta
     1441 gctctcacaa tcatatccct ggatacaagg ttcagacgaa cggaccttgg atgcaggtac
     1501 cactagaagt gaagagagaa gcttgcccag ggactagcgt gatcattgat ggcaactgtg
     1561 atggacgggg aaaatcaacc agatccacca cggatagcgg gaaagttatt cctgaatggt
     1621 gttgccgctc ctgcacaatg ccgcctgtga gcttccatgg tagtgatggg tgttggtatc
     1681 ccatggaaat taggccaagg aaaacgcatg aaagccatct ggtgcgctcc tgggttacag
     1741 cttgaataaa tcgataccga gcgctgctcg agagatctac gggtggcatc cctgtgaccc
     1801 ctccccagtg cctctcctgg ccctggaagt tgccactcca gtgcccacca gccttgtcct
     1861 aataaaatta agttgcatca ttttgtctga ctaggtgtcc ttctataata ttatggggtg
     1921 gaggggggtg gtatggagca aggggcaagt tgggaagaca acctgtaggg cctgcggggt
     1981 ctattgggaa ccaagctgga gtgcagtggc acaatcttgg ctcactgcaa tctccgcctc
     2041 ctgggttcaa gcgattctcc tgcctcagcc tcccgagttg ttgggattcc aggcatgcat
     2101 gaccaggctc agctaatttt tgtttttttg gtagagacgg ggtttcacca tattggccag
     2161 gctggtctcc aactcctaat ctcaggtgat ctacccacct tggcctccca aattgctggg
     2221 attacaggcg tgaaccactg ctcccttccc tgtccttctg attttgtagg taaccacgtg
     2281 cggaccgagc ggccgcagga acccctagtg atggagttgg ccactccctc tctgcgcgct
     2341 cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg
     2401 gcctcagtga gcgagcgagc gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc
     2461 cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct
     2521 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     2581 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     2641 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     2701 ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct
     2761 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     2821 tccaaactgg aacaacactc aaccctatct cgggctattc ttttgattta taagggattt
     2881 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     2941 ttaacaaaat attaacgttt acaattttat ggtgcactct cagtacaatc tgctctgatg
     3001 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     3061 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     3121 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
     3181 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     3241 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     3301 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     3361 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     3421 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     3481 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     3541 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     3601 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     3661 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     3721 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     3781 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     3841 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     3901 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     3961 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     4021 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     4081 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     4141 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     4201 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     4261 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     4321 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     4381 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     4441 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     4501 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     4561 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     4621 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     4681 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     4741 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     4801 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     4861 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     4921 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     4981 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgt
//
