LOCUS       62056_2525        5842 bp DNA     circular SYN 30-NOV-2021
DEFINITION  Cloning vector pAAV-TRE-NS1-dTomato, complete sequence.
ACCESSION   MZ708021
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5842)
  AUTHORS   Li E, Guo J, Oh SJ, Luo Y, Oliveros HC, Du W, Arano R, Kim Y, Chen 
            YT, Eitson J, Lin DT, Li Y, Roberts T, Schoggins JW, Xu W.
  TITLE     Anterograde transneuronal tracing and genetic control with 
            engineered yellow fever vaccine YFV-17D
  JOURNAL   Nat Methods (2021) In press
  PUBMED    34824475
REFERENCE   2  (bases 1 to 5842)
  AUTHORS   Xu W.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2021) Neuroscience, UT Southwestern Medical 
            Center, Dallas, TX 75229, USA
REFERENCE   3  (bases 1 to 5842)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Methods
            (2021) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (03-AUG-2021) Neuroscience, UT Southwestern Medical Center, Dallas, 
            TX 75229, USA"
FEATURES             Location/Qualifiers
     source          1..5842
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     polyA_signal    complement(164..245)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(263..964)
                     /label=dTomato
                     /note="dimeric variant of DsRed fluorescent protein (Shaner
                     et al., 2004)"
     CDS             complement(962..982)
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     promoter        996..1383
                     /label=bidirectional TRE promoter
                     /note="Tet-responsive bidirectional promoter PTight-BI, 
                     consisting of seven tet operator sequences flanked on each 
                     side by the minimal CMV promoter"
     CDS             1420..2553
                     /codon_start=1
                     /transl_table=11
                     /product="NS1"
                     /label=NS1
                     /protein_id="UBZ25898.1"
                     /translation="MNMTMSMSMILVGVIMMFLSLGVGADQGCAINFGKRELKCGDGIF
                     IFRDSDDWLNKYSYYPEDPVKLASIVKASFEEGKCGLNSVDSLEHEMWRSRADEINAIF
                     EENEVDISVVVQDPKNVYQRGTHPFSRIRDGLQYGWKTWGKNLVFSPGRKNGSFIIDGK
                     SRKECPFSNRVWNSFQIEEFGTGVFTTRVYMDAVFEYTIDCDGSILGAAVNGKKSAHGS
                     PTFWMGSHEVNGTWMIHTLEALDYKECEWPLTHTIGTSVEESEMFMPRSIGGPVSSHNH
                     IPGYKVQTNGPWMQVPLEVKREACPGTSVIIDGNCDGRGKSTRSTTDSGKVIPEWCCRS
                     CTMPPVSFHGSDGCWYPMEIRPRKTHESHLVRSWVTA"
     polyA_signal    2589..3065
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     repeat_region   3105..3245
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      3320..3775
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4057..4161
                     /label=AmpR promoter
     CDS             4162..5019
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      5193..5781
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtatttg tcggcagtga aaaaaatgct
      181 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
      241 aagttaacaa caacaattgt tacttgtaca gctcgtccat gccgtacagg aacaggtggt
      301 ggcggccctc ggagcgctcg tactgttcca cgatggtgta gtcctcgttg tgggaggtga
      361 tgtccagctt ggtgtccacg tagtagtagc cgggcagttg cacgggcttc ttggccatgt
      421 agatggtctt gaactccacc aggtagtggc cgccgtcctt cagcttcagg gcctggtgga
      481 tctcgccctt cagcacgccg tcgcgggggt acaggcgctc ggtggaggcc tcccagccca
      541 tggtcttctt ctgcattacg gggccgtcgg gggggaagtt ggtgccgcgc atcttcacct
      601 tgtagatcag cgtgccgtcc tgcagggagg agtcctgggt cacggtcacc agaccgccgt
      661 cctcgaagtt catcacgcgc tcccacttga agccctcggg gaaggacagc ttcttgtaat
      721 cggggatgtc ggcggggtgc ttcacgtacg ccttggagcc gtacatgaac tggggggaca
      781 ggatgtccca ggcgaagggc agggggccgc ccttggtcac cttcagcttg gcggtctggg
      841 tgccctcgta ggggcggccc tcgccctcgc cctcgatctc gaactcgtgg ccgttcatgg
      901 agccctccat gcgcaccttg aagcgcatga actctttgat gacctcctcg cccttgctca
      961 ccaccttcct cttcttcttg ggcatgaatt ctccaggcga tctgacggtt cactaaacga
     1021 gctctgctta tataggcctc ccaccgtaca cgccacctcg acatactcga gtttactccc
     1081 tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag aacgatgtcg
     1141 agtttactcc ctatcagtga tagagaacgt atgtcgagtt tactccctat cagtgataga
     1201 gaacgtatgt cgagtttact ccctatcagt gatagagaac gtatgtcgag tttatcccta
     1261 tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa cgtatgtcga
     1321 ggtaggcgtg tacggtggga ggcctatata agcagagctc gtttagtgaa ccgtcagatc
     1381 gcctggagat ttcgagctcg gtaccaataa tcagccacca tgaacatgac aatgtccatg
     1441 agcatgatct tggtaggagt gatcatgatg tttttgtctc taggagttgg ggcggatcaa
     1501 ggatgcgcca tcaactttgg caagagagag ctcaagtgcg gagatggtat cttcatattt
     1561 agagactctg atgactggct gaacaagtac tcatactatc cagaagatcc tgtgaagctt
     1621 gcatcaatag tgaaagcctc ttttgaagaa gggaagtgtg gcctaaattc agttgactcc
     1681 cttgagcatg agatgtggag aagcagggca gatgagatca atgccatttt tgaggaaaac
     1741 gaggtggaca tttctgttgt cgtgcaggat ccaaagaatg tttaccagag aggaactcat
     1801 ccattttcca gaattcggga tggtctgcag tatggttgga agacttgggg taagaacctt
     1861 gtgttctccc cagggaggaa gaatggaagc ttcatcatag atggaaagtc caggaaagaa
     1921 tgcccgtttt caaaccgggt ctggaattct ttccagatag aggagtttgg gacgggagtg
     1981 ttcaccacac gcgtgtacat ggacgcagtc tttgaataca ccatagactg cgatggatct
     2041 atcttgggtg cagcggtgaa cggaaaaaag agtgcccatg gctctccaac attttggatg
     2101 ggaagtcatg aagtaaatgg gacatggatg atccacacct tggaggcatt agattacaag
     2161 gagtgtgagt ggccactgac acatacgatt ggaacatcag ttgaagagag tgaaatgttc
     2221 atgccgagat caatcggagg cccagttagc tctcacaatc atatccctgg atacaaggtt
     2281 cagacgaacg gaccttggat gcaggtacca ctagaagtga agagagaagc ttgcccaggg
     2341 actagcgtga tcattgatgg caactgtgat ggacggggaa aatcaaccag atccaccacg
     2401 gatagcggga aagttattcc tgaatggtgt tgccgctcct gcacaatgcc gcctgtgagc
     2461 ttccatggta gtgatgggtg ttggtatccc atggaaatta ggccaaggaa aacgcatgaa
     2521 agccatctgg tgcgctcctg ggttacagct tgaataaatc gataccgagc gctgctcgag
     2581 agatctacgg gtggcatccc tgtgacccct ccccagtgcc tctcctggcc ctggaagttg
     2641 ccactccagt gcccaccagc cttgtcctaa taaaattaag ttgcatcatt ttgtctgact
     2701 aggtgtcctt ctataatatt atggggtgga ggggggtggt atggagcaag gggcaagttg
     2761 ggaagacaac ctgtagggcc tgcggggtct attgggaacc aagctggagt gcagtggcac
     2821 aatcttggct cactgcaatc tccgcctcct gggttcaagc gattctcctg cctcagcctc
     2881 ccgagttgtt gggattccag gcatgcatga ccaggctcag ctaatttttg tttttttggt
     2941 agagacgggg tttcaccata ttggccaggc tggtctccaa ctcctaatct caggtgatct
     3001 acccaccttg gcctcccaaa ttgctgggat tacaggcgtg aaccactgct cccttccctg
     3061 tccttctgat tttgtaggta accacgtgcg gaccgagcgg ccgcaggaac ccctagtgat
     3121 ggagttggcc actccctctc tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt
     3181 cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc gcagctgcct
     3241 gcaggggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
     3301 acgtcaaagc aaccatagta cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     3361 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     3421 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     3481 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgatttgggt
     3541 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     3601 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     3661 ggctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     3721 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac aattttatgg
     3781 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca
     3841 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
     3901 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
     3961 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     4021 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     4081 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     4141 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     4201 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     4261 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     4321 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     4381 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     4441 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     4501 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     4561 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     4621 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     4681 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     4741 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     4801 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     4861 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     4921 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     4981 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     5041 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     5101 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     5161 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     5221 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     5281 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
     5341 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     5401 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     5461 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     5521 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     5581 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     5641 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     5701 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     5761 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     5821 tgctggcctt ttgctcacat gt
//
