LOCUS       V015509                 7293 bp    DNA     circular SYN 30-NOV-2021
DEFINITION  Exported.
ACCESSION   V015509
VERSION     V015509
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7293)
  AUTHORS   Li E, Guo J, Oh SJ, Luo Y, Oliveros HC, Du W, Arano R, Kim Y, Chen
            YT, Eitson J, Lin DT, Li Y, Roberts T, Schoggins JW, Xu W.
  TITLE     Anterograde transneuronal tracing and genetic control with
            engineered yellow fever vaccine YFV-17D
  JOURNAL   Nat Methods (2021) In press
   PUBMED   34824475
REFERENCE   2  (bases 1 to 7293)
  AUTHORS   Xu W.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2021) Neuroscience, UT Southwestern Medical
            Center, Dallas, TX 75229, USA
REFERENCE   3  (bases 1 to 7293)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Methods
            (2021) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (03-AUG-2021) Neuroscience, UT Southwestern Medical Center, Dallas,
            TX 75229, USA"
FEATURES             Location/Qualifiers
     source          1..7293
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..141
                     /label="AAV2 ITR"
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
     polyA_signal    complement(164..245)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        261..575
                     /label="tight TRE promoter"
                     /note="Tet-responsive promoter PTight, consisting of seven
                     tet operator sequences followed by the minimal CMV
                     promoter"
     CDS             612..4001
                     /note="Genome polyprotein from Yellow fever virus (isolate
                     Peru/1899/1981). Accession#: P29165"
                     /label="Genome polyprotein"
     polyA_signal    4040..4516
                     /label="hGH poly(A) signal"
                     /note="human growth hormone polyadenylation signal"
     repeat_region   4556..4696
                     /label="AAV2 ITR"
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
     rep_origin      4771..5226
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5508..5612
                     /label="AmpR promoter"
     CDS             5613..6470
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      6644..7232
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtatttg tcggcagtga aaaaaatgct
      181 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
      241 aagttaacaa caacaatttc gagtttactc cctatcagtg atagagaacg tatgtcgagt
      301 ttactcccta tcagtgatag agaacgatgt cgagtttact ccctatcagt gatagagaac
      361 gtatgtcgag tttactccct atcagtgata gagaacgtat gtcgagttta ctccctatca
      421 gtgatagaga acgtatgtcg agtttatccc tatcagtgat agagaacgta tgtcgagttt
      481 actccctatc agtgatagag aacgtatgtc gaggtaggcg tgtacggtgg gaggcctata
      541 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag atttcgagct cggtaccaat
      601 aatcagccac catgtccggt cgtaaagccc agggcaaaac cctaggcgta aatatggtcc
      661 gacgcggagt ccgctcattg tcgaacaaaa taaaacaaaa aacaaaacaa attggaaaca
      721 gacctggacc ttcaagaggt gttcaaggat ttatcttttt ctttttgttc aacattttga
      781 ctggaaaaaa gatcacagcc cacctaaaga ggttgtggaa aatgctggac ccaagacaag
      841 gcttggctgt tctaaggaaa gtcaagagag tggtggccag tttgatgaga ggattgtcct
      901 caaggaaacg ccgttcccat gatgttctga ctgtgcaatt cctaattttg ggaatgctgt
      961 tgatgacggg tggagtgacc ttggtgcgga aaaacagatg gttgctccta aatgtgacat
     1021 ctgaggacct cgggaaaaca ttctctgtgg gcacaggcaa ctgcacaaca aacattttgg
     1081 aagccaagta ctggtgccca gactcaatgg aatacaactg tcccaatctc agtccaagag
     1141 aggagccaga tgacattgat tgctggtgct atggggtgga aaacgttaga gtcgcatatg
     1201 gtaagtgtga ctcagcaggc aggtctagga ggtcaagaag ggccattgac ttgcctacgc
     1261 atgaaaacca tggtttgaag acccggcaag aaaaatggat gactggaaga atgggtgaaa
     1321 ggcaactcca aaagattgag agatggttcg tgaggaaccc cttttttgca gtgacggctc
     1381 tgaccattgc ctaccttgtg ggaagcaaca tgacgcaacg agtcgtgatt gccctactgg
     1441 tcttggctgt tggtccggcc tactcagctc actgcattgg aattactgac agggatttca
     1501 ttgagggggt gcatggagga acttgggttt cagctaccct ggagcaagac aagtgtgtca
     1561 ctgttatggc ccctgacaag ccttcattgg acatctcact agagacagta gccattgata
     1621 gacctgctga ggtgaggaaa gtgtgttaca atgcagttct cactcatgtg aagattaatg
     1681 acaagtgccc cagcactgga gaggcccacc tagccgaaga gaacgaaggg gacaatgcgt
     1741 gcaagcgcac ttattctgat agaggctggg gcaatggctg tggcctattt gggaaaggga
     1801 gcattgtggc atgcgccaaa ttcacttgtg ccaaatccat gagtttgttt gaggttgatc
     1861 agaccaaaat tcagtatgtc atcagagcac aattgcatgt aggggccaag caggaaaatt
     1921 ggaataccga cattaagact ctcaagtttg atgccctgtc aggctcccag gaagtcgagt
     1981 tcattgggta tggaaaagct acactggaat gccaggtgca aactgcggtg gactttggta
     2041 acagttacat cgctgagatg gaaacagaga gctggatagt ggacagacag tgggcccagg
     2101 acttgaccct gccatggcag agtggaagtg gcggggtgtg gagagagatg catcatcttg
     2161 tcgaatttga acctccgcat gccgccacta tcagagtact ggccctggga aaccaggaag
     2221 gctccttgaa aacagctctt actggcgcaa tgagggttac aaaggacaca aatgacaaca
     2281 acctttacaa actacatggt ggacatgttt cttgcagagt gaaattgtca gctttgacac
     2341 tcaaggggac atcctacaaa atatgcactg acaaaatgtt ttttgtcaag aacccaactg
     2401 acactggcca tggcactgtt gtgatgcagg tgaaagtgtc aaaaggagcc ccctgcagga
     2461 ttccagtgat agtagctgat gatcttacag cggcaatcaa taaaggcatt ttggttacag
     2521 ttaaccccat cgcctcaacc aatgatgatg aagtgctgat tgaggtgaac ccaccttttg
     2581 gagacagcta cattatcgtt gggagaggag attcacgtct cacttaccag tggcacaaag
     2641 agggaagctc aataggaaag ttgttcactc agaccatgaa aggcgtggaa cgcctggccg
     2701 tcatgggaga caccgcctgg gatttcagct ccgctggagg gttcttcact tcggttggga
     2761 aaggaattca tacggtgttt ggctctgcct ttcaggggct atttggcggc ttgaactgga
     2821 taacaaaggt catcatgggg gcggtactta tatgggttgg catcaacaca agaaacatga
     2881 caatgtccat gagcatgatc ttggtaggag tgatcatgat gtttttgtct ctaggagttg
     2941 gggcggatca aggatgcgcc atcaactttg gcaagagaga gctcaagtgc ggagatggta
     3001 tcttcatatt tagagactct gatgactggc tgaacaagta ctcatactat ccagaagatc
     3061 ctgtgaagct tgcatcaata gtgaaagcct cttttgaaga agggaagtgt ggcctaaatt
     3121 cagttgactc ccttgagcat gagatgtgga gaagcagggc agatgagatc aatgccattt
     3181 ttgaggaaaa cgaggtggac atttctgttg tcgtgcagga tccaaagaat gtttaccaga
     3241 gaggaactca tccattttcc agaattcggg atggtctgca gtatggttgg aagacttggg
     3301 gtaagaacct tgtgttctcc ccagggagga agaatggaag cttcatcata gatggaaagt
     3361 ccaggaaaga atgcccgttt tcaaaccggg tctggaattc tttccagata gaggagtttg
     3421 ggacgggagt gttcaccaca cgcgtgtaca tggacgcagt ctttgaatac accatagact
     3481 gcgatggatc tatcttgggt gcagcggtga acggaaaaaa gagtgcccat ggctctccaa
     3541 cattttggat gggaagtcat gaagtaaatg ggacatggat gatccacacc ttggaggcat
     3601 tagattacaa ggagtgtgag tggccactga cacatacgat tggaacatca gttgaagaga
     3661 gtgaaatgtt catgccgaga tcaatcggag gcccagttag ctctcacaat catatccctg
     3721 gatacaaggt tcagacgaac ggaccttgga tgcaggtacc actagaagtg aagagagaag
     3781 cttgcccagg gactagcgtg atcattgatg gcaactgtga tggacgggga aaatcaacca
     3841 gatccaccac ggatagcggg aaagttattc ctgaatggtg ttgccgctcc tgcacaatgc
     3901 cgcctgtgag cttccatggt agtgatgggt gttggtatcc catggaaatt aggccaagga
     3961 aaacgcatga aagccatctg gtgcgctcct gggttacagc ttgaataaat cgataccgag
     4021 cgctgctcga gagatctacg ggtggcatcc ctgtgacccc tccccagtgc ctctcctggc
     4081 cctggaagtt gccactccag tgcccaccag ccttgtccta ataaaattaa gttgcatcat
     4141 tttgtctgac taggtgtcct tctataatat tatggggtgg aggggggtgg tatggagcaa
     4201 ggggcaagtt gggaagacaa cctgtagggc ctgcggggtc tattgggaac caagctggag
     4261 tgcagtggca caatcttggc tcactgcaat ctccgcctcc tgggttcaag cgattctcct
     4321 gcctcagcct cccgagttgt tgggattcca ggcatgcatg accaggctca gctaattttt
     4381 gtttttttgg tagagacggg gtttcaccat attggccagg ctggtctcca actcctaatc
     4441 tcaggtgatc tacccacctt ggcctcccaa attgctggga ttacaggcgt gaaccactgc
     4501 tcccttccct gtccttctga ttttgtaggt aaccacgtgc ggaccgagcg gccgcaggaa
     4561 cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg
     4621 cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg
     4681 cgcagctgcc tgcaggggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     4741 tcacaccgca tacgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg
     4801 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     4861 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     4921 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     4981 ttgatttggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     5041 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     5101 accctatctc gggctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     5161 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta
     5221 caattttatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc
     5281 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     5341 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     5401 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     5461 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     5521 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     5581 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     5641 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     5701 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     5761 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     5821 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     5881 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     5941 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     6001 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     6061 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     6121 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     6181 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     6241 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     6301 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     6361 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     6421 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     6481 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     6541 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     6601 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     6661 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     6721 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     6781 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     6841 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     6901 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     6961 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     7021 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     7081 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     7141 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     7201 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     7261 tcctggcctt ttgctggcct tttgctcaca tgt
//
