LOCUS       62056_2515        5847 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5847)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5847
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     enhancer        221..524
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        525..728
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          877..1255
                     /label=chimeric intron
                     /note="fusion between human cytomegalovirus intron A and
                     human beta-globin intron 2"
     CDS             1337..2029
                     /codon_start=1
                     /label=ZsGreen1
                     /note="Zoanthus green fluorescent protein"
                     /translation="MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFKGKQAIN
                     LCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDGAV
                     CICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGILKG
                     DVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWHLTEH
                     AIASGSALP"
     polyA_signal    2041..2517
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     promoter        2628..3037
                     /label=TBG promoter
                     /note="promoter from the human thyroxine-binding globulin
                     gene (Yan et al., 2012)"
     repeat_region   3110..3250
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      3325..3780
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4062..4166
                     /label=AmpR promoter
     CDS             4167..5024
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5198..5786
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtggagc tagttattaa tagtaatcaa
      181 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
      241 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
      301 ttcccatagt aacgtcaata gggactttcc attgacgtca atgggtggag tatttacggt
      361 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
      421 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
      481 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
      541 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
      601 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
      661 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
      721 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
      781 tccatagaag acaccgggac cgatccagcc tccgcggatt cgaatcccgg ccgggaacgg
      841 tgcattggaa cgcggattcc ccgtgccaag agtgacgtaa gtaccgccta tagagtctat
      901 aggcccacaa aaaatgcttt cttcttttaa tatacttttt tgtttatctt atttctaata
      961 ctttccctaa tctctttctt tcagggcaat aatgatacaa tgtatcatgc ctctttgcac
     1021 cattctaaag aataacagtg ataatttctg ggttaaggca atagcaatat ttctgcatat
     1081 aaatatttct gcatataaat tgtaactgat gtaagaggtt tcatattgct aatagcagct
     1141 acaatccagc taccattctg cttttatttt atggttggga taaggctgga ttattctgag
     1201 tccaagctag gcccttttgc taatcatgtt catacctctt atcttcctcc cacagctcct
     1261 gggcaacgtg ctggtctgtg tgctggccca tcactttggc aaagaattgg gattcgaaca
     1321 tcgattgaat tccaccatgg cccagtccaa gcacggcctg accaaggaga tgaccatgaa
     1381 gtaccgcatg gagggctgcg tggacggcca caagttcgtg atcaccggcg agggcatcgg
     1441 ctaccccttc aagggcaagc aggccatcaa cctgtgcgtg gtggagggcg gccccttgcc
     1501 cttcgccgag gacatcttgt ccgccgcctt catgtacggc aaccgcgtgt tcaccgagta
     1561 cccccaggac atcgtcgact acttcaagaa ctcctgcccc gccggctaca cctgggaccg
     1621 ctccttcctg ttcgaggacg gcgccgtgtg catctgcaac gccgacatca ccgtgagcgt
     1681 ggaggagaac tgcatgtacc acgagtccaa gttctacggc gtgaacttcc ccgccgacgg
     1741 ccccgtgatg aagaagatga ccgacaactg ggagccctcc tgcgagaaga tcatccccgt
     1801 gcccaagcag ggcatcttga agggcgacgt gagcatgtac ctgctgctga aggacggtgg
     1861 ccgcttgcgc tgccagttcg acaccgtgta caaggccaag tccgtgcccc gcaagatgcc
     1921 cgactggcac ttcatccagc acaagctgac ccgcgaggac cgcagcgacg ccaagaacca
     1981 gaagtggcac ctgaccgagc acgccatcgc ctccggctcc gccttgccct aaagatctac
     2041 gggtggcatc cctgtgaccc ctccccagtg cctctcctgg ccctggaagt tgccactcca
     2101 gtgcccacca gccttgtcct aataaaatta agttgcatca ttttgtctga ctaggtgtcc
     2161 ttctataata ttatggggtg gaggggggtg gtatggagca aggggcaagt tgggaagaca
     2221 acctgtaggg cctgcggggt ctattgggaa ccaagctgga gtgcagtggc acaatcttgg
     2281 ctcactgcaa tctccgcctc ctgggttcaa gcgattctcc tgcctcagcc tcccgagttg
     2341 ttgggattcc aggcatgcat gaccaggctc agctaatttt tgtttttttg gtagagacgg
     2401 ggtttcacca tattggccag gctggtctcc aactcctaat ctcaggtgat ctacccacct
     2461 tggcctccca aattgctggg attacaggcg tgaaccactg ctcccttccc tgtccttctg
     2521 attttgtagg taaccagtgg ccaactccat cactaggggt tcctgcggcc ggtcgcgtct
     2581 agtactagtg ggctggaagc tacctttgac atcatttcct ctgcgaatgc atgtataatt
     2641 tctacagaac ctattagaaa ggatcaccca gcctctgctt ttgtacaact ttcccttaaa
     2701 aaactgccaa ttccactgct gtttggccca atagtgagaa ctttttcctg ctgcctcttg
     2761 gtgcttttgc ctatggcccc tattctgcct gctgaagaca ctcttgccag catggactta
     2821 aacccctcca gctctgacaa tcctctttct cttttgtttt acatgaaggg tctggcagcc
     2881 aaagcaatca ctcaaagttc aaaccttatc attttttgct ttgttcctct tggccttggt
     2941 tttgtacatc agctttgaaa ataccatccc agggttaatg ctggggttaa tttataacta
     3001 agagtgctct agttttgcaa tacaggacat gctataagcg ggatccgcga gctgcaggac
     3061 tctaactcga gttagagtcc tgcagctcgc tttttaagct tgcggccgca ggaaccccta
     3121 gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca
     3181 aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc
     3241 tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
     3301 cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     3361 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     3421 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     3481 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     3541 tgggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     3601 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     3661 tctcgggcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     3721 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt
     3781 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc
     3841 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
     3901 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
     3961 gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     4021 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     4081 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     4141 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     4201 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     4261 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     4321 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     4381 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     4441 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     4501 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     4561 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     4621 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     4681 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     4741 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     4801 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     4861 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     4921 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     4981 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     5041 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     5101 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     5161 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     5221 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     5281 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     5341 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     5401 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     5461 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     5521 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     5581 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     5641 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     5701 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     5761 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     5821 ccttttgctg gccttttgct cacatgt
//
