LOCUS       62056_2510        4529 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4529)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4529
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        194..603
                     /label=TBG promoter
                     /note="promoter from the human thyroxine-binding globulin
                     gene (Yan et al., 2012)"
     CDS             783..1499
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     polyA_signal    1638..1759
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   1792..1932
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      2007..2462
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2744..2848
                     /label=AmpR promoter
     CDS             2849..3706
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3880..4468
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccggtc gcgtctagta ctagtgggct ggaagctacc tttgacatca
      181 tttcctctgc gaatgcatgt ataatttcta cagaacctat tagaaaggat cacccagcct
      241 ctgcttttgt acaactttcc cttaaaaaac tgccaattcc actgctgttt ggcccaatag
      301 tgagaacttt ttcctgctgc ctcttggtgc ttttgcctat ggcccctatt ctgcctgctg
      361 aagacactct tgccagcatg gacttaaacc cctccagctc tgacaatcct ctttctcttt
      421 tgttttacat gaagggtctg gcagccaaag caatcactca aagttcaaac cttatcattt
      481 tttgctttgt tcctcttggc cttggttttg tacatcagct ttgaaaatac catcccaggg
      541 ttaatgctgg ggttaattta taactaagag tgctctagtt ttgcaataca ggacatgcta
      601 taaaaatgga aagatggtac cgaggagatc tgccgccgcg atcgccggcg gatccgcatt
      661 gcggccgcag acatatgtct cacgcttaac tagctagcgg accgacgcgt ggaagcggag
      721 ctactaactt cagcctgctg aagcaggctg gagacgtgga ggagaaccct ggacctgtcg
      781 acatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
      841 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
      901 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
      961 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga
     1021 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     1081 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     1141 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     1201 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
     1261 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
     1321 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     1381 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
     1441 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt
     1501 aagatatccg atccaccgga tctagataac tgatcataat cagccatacc acatttgtag
     1561 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
     1621 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     1681 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     1741 aactcatcaa tgtatcttaa cgcggtaacc acgtgcggac ccaatggccg caggaacccc
     1801 tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag gccgggcgac
     1861 caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag cgagcgcgca
     1921 gctgcctgca ggggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     1981 accgcatacg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa gcgcggcggg
     2041 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccttagcgc ccgctccttt
     2101 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     2161 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     2221 tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     2281 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     2341 tatctcgggc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     2401 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat
     2461 tttatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
     2521 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
     2581 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
     2641 acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     2701 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     2761 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     2821 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     2881 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     2941 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     3001 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     3061 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     3121 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     3181 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     3241 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     3301 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     3361 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     3421 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     3481 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     3541 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     3601 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     3661 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     3721 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     3781 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     3841 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     3901 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     3961 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     4021 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     4081 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     4141 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     4201 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     4261 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     4321 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     4381 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     4441 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     4501 ggccttttgc tggccttttg ctcacatgt
//
