LOCUS       pAAV-Smc1a(rat)-FLAG    8356 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V021180
VERSION     V021180
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     repeat_region   1..141
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     enhancer        221..524
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        525..727
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     intron          876..1254
                     /note="fusion between human cytomegalovirus intron A and
                     human β-globin intron 2"
                     /label="chimeric intron"
     CDS             1343..5041
                     /label="Smc1a(NM_031683)"
                     /note="Smc1a(NM_031683)"
                     /gene="Smc1a"
     CDS             5042..5065
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     polyA_signal    5103..5579
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     repeat_region   5619..5759
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     rep_origin      5834..6289
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        6571..6675
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             6676..7536
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      7707..8295
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtggagc tagttattaa tagtaatcaa
      181 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
      241 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
      301 ttcccatagt aacgtcaata gggactttcc attgacgtca atgggtggag tatttacggt
      361 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
      421 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
      481 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
      541 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
      601 ttgacgtcaa tgggagtttg ttttgcacca aaatcaacgg gactttccaa aatgtcgtaa
      661 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
      721 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
      781 ccatagaaga caccgggacc gatccagcct ccgcggattc gaatcccggc cgggaacggt
      841 gcattggaac gcggattccc cgtgccaaga gtgacgtaag taccgcctat agagtctata
      901 ggcccacaaa aaatgctttc ttcttttaat atactttttt gtttatctta tttctaatac
      961 tttccctaat ctctttcttt cagggcaata atgatacaat gtatcatgcc tctttgcacc
     1021 attctaaaga ataacagtga taatttctgg gttaaggcaa tagcaatatt tctgcatata
     1081 aatatttctg catataaatt gtaactgatg taagaggttt catattgcta atagcagcta
     1141 caatccagct accattctgc ttttatttta tggttgggat aaggctggat tattctgagt
     1201 ccaagctagg cccttttgct aatcatgttc atacctctta tcttcctccc acagctcctg
     1261 ggcaacgtgc tggtctgtgt gctggcccat cactttggca aagaattggg attcgaacat
     1321 cgattgaatt ccccggggat ccatgggttt cctgaaactg attgagattg agaactttaa
     1381 atcgtacaag ggtcgacaaa ttatcggacc atttcagagg ttcaccgcca tcattggacc
     1441 caatggctct ggtaagtcaa atctcatgga tgccatcagc tttgtgcttg gtgaaaaaac
     1501 cagcaacctg agggtaaaga ccctgaggga cctgatacac ggagctcctg tgggcaagcc
     1561 ggctgccaac cgagcctttg tcagcatggt ctactccgag gagggtgctg aggaccgcac
     1621 ctttgcccga gtcattgtag ggggctcttc tgagtacaag atcaataaca aagttgtcca
     1681 gctacatgaa tacagtgaag agttagagaa gttgggcatt ctcatcaaag ctcgtaactt
     1741 cctcgtcttc cagggtgcag tggaatctat tgccatgaag aaccccaagg agaggacagc
     1801 tttatttgaa gaaattagtc gctctgggga acttgcacag gagtatgaca agcgaaagaa
     1861 agaaatggtg aaggctgaag aagacacaca gtttaattat catcgcaaga aaaatattgc
     1921 agctgaacgc aaggaggcca aacaagaaaa ggaagaggct gaccgctacc agcgcctgaa
     1981 ggatgaggta gtacgcgcac aggtacaatt gcagcttttt aaactctacc acaacgaagt
     2041 ggagattgag aagctcaaca aggaactggc ctcgaagaac aaggaaattg agaaggacaa
     2101 aaagcggatg gacaaggtag aggatgaact gaaggagaag aagaaagagc tgggcaaaat
     2161 gatgcgggaa cagcagcaga ttgagaagga gatcaaggaa aaggactcag aattgaatca
     2221 gaagaggcct cagtacatca aagcaaaaga gaatacatcc cacaaaatca agaagcttga
     2281 agcagccaag aaatctctgc agaatgctca gaaacattat aagaaacgta aaggtgacat
     2341 ggatgagctg gagaaagaga tgctgtcagt ggagaaggcc cgacaggagt ttgaagagcg
     2401 gatggaggaa gagagtcaga gtcaaggcag agacttgacc ctggaggaga accaggtaaa
     2461 gaaataccac cgattaaaag aggaagctag caagagagca gccactttgg ctcaggagct
     2521 ggagaagttt aatcgagacc agaaagctga ccaggaccgt ctagatctag aagagcggaa
     2581 gaaagtagag acagaggcca agatcaagca aaaactgcgg gagattgaag agaatcagaa
     2641 acggattgag aagctagagg aatatattac cactagcaag cagtctctag aggaacagaa
     2701 gaagctagag ggagaattga cagaagaagt agagatggcc aagcgtcgta ttgatgagat
     2761 caataaggag ctgaaccagg tgatggagca attgggggat gcgcgcattg accggcagga
     2821 gagcagccgc cagcagagga aggcagaaat aatggaaagc atcaagcgtc tctaccctgg
     2881 ttctgtgtat ggccgcctca ttgacctctg ccagcctaca caaaagaagt atcagattgc
     2941 tgtgaccaag gttttaggaa agaacatgga tgctattatt gtagactccg agaagacagg
     3001 tcgggattgt attcagtata tcaaggagca acgtggagag cctgagacct tcttgcctct
     3061 tgactacctg gaggtgaaac ctactgatga gaaactccga gaactgaagg gggccaagct
     3121 agtgattgat gtgattcgct atgagccacc tcacatcaaa aaagctctgc agtatgcttg
     3181 tggcaatgcc cttgtttgtg acaatgtgga ggatgcgcga cgaattgcct ttggaggtca
     3241 ccaacgccac aagacagtgg cattggatgg caccctgttc cagaagtcag gagttatctc
     3301 tggtggagcc agtgatctca aggcaaaggc acgacgctgg gatgagaagg cagtagacaa
     3361 actaaaagaa aaaaaagaac gtttgacgga ggagctaaaa gagcagatga aggcaaaacg
     3421 aaaagaggca gagctgcgtc aggtgcagtc tcaggcccat gggctacaaa tgcggctgaa
     3481 gtactcacag agtgacctag aacagactaa gacaaggcat cttgccctga atctccagga
     3541 aaaatctaag ctggagagtg aactagccaa cttcgggcct cgaattaatg atatcaagag
     3601 aatcatacag agccgagaga gggaaatgaa agacttgaag gagaagatga accaggtgga
     3661 agatgaggtg tttgaagagt tttgtcggga gattggtgtg cgtaacatcc gcgaatttga
     3721 ggaagaaaag gtgaagcggc agaatgaaat tgccaagaaa cgcttggaat ttgagaatca
     3781 gaagactcgc ttgggcatcc agttggattt tgaaaagaat cagttgaagg aggaccagga
     3841 taaagtacac atgtgggagc agacagtaaa aaaggatgaa aatgagatag aaaagcttaa
     3901 gaaggaggag caaagacaca tgaagataat agatgaaacc atggctcagc tccaagacct
     3961 taagaatcag cacctggcca aaaagtcaga agtgaatgac aagaatcatg agatggaaga
     4021 aattcgcaag aagcttgggg gtgccaataa ggaaatgact cacttacaga aggaagtgac
     4081 agccattgag accaagcttg aacagaagcg cagtgaccgc cacaacctgc tacaggcctg
     4141 taagatgcag gacatcaaat tgccactgtc aaagggcacc atggatgata ttagtcagga
     4201 agaaggtggc tcccagggag aagagtcagt gagtggttcc cagagaacat ctagtatcta
     4261 tgcacgagag gccctcattg agattgacta cggtgacctg tgtgaagatc tgaaggatgc
     4321 acaggcggaa gaagaaatca agcaagagat gaacacactc cagcaaaagc tgaatgagca
     4381 gcagagtgtg cttcagagga ttgctgcccc taacatgaag gccatggaaa agctggaaag
     4441 tgtccgagac aagttccagg agacctcaga tgaatttgag gcagcacgaa agcgagcaaa
     4501 gaaagccaag caggcatttg aacagataaa gaaggagcgc tttgaccgat tcaatgcttg
     4561 ttttgaatct gtggctacca atattgatga gatctacaag gctctgtccc gtaacagcag
     4621 tgcccaggca ttcttgggcc ccgagaatcc tgaagagccc tatttggatg gtatcaacta
     4681 caactgtgta gctcctggca aacgtttccg gcctatggac aatttgtcag gcggggagaa
     4741 gacagttgcc gcactggcct tgctttttgc cattcacagc tacaagccag cccccttctt
     4801 cgtcctggat gagattgatg ctgccttgga taacaccaac attggcaagg tggcaaatta
     4861 catcaaggag cagtcaactt gcaacttcca ggccattgtt atctctctca aggaggaatt
     4921 ctacaccaag gctgagagcc tcattggagt ctatcctgag caaggagact gtgtgatcag
     4981 caaagtcctg accttcgacc tcaccaagta tccagatgcc aaccctaacc ccaatgaaca
     5041 agattacaag gacgacgatg acaagtaagc ttgcctcgag cagcgctgct cgagagatct
     5101 acgggtggca tccctgtgac ccctccccag tgcctctcct ggccctggaa gttgccactc
     5161 cagtgcccac cagccttgtc ctaataaaat taagttgcat cattttgtct gactaggtgt
     5221 ccttctataa tattatgggg tggagggggg tggtatggag caaggggcaa gttgggaaga
     5281 caacctgtag ggcctgcggg gtctattggg aaccaagctg gagtgcagtg gcacaatctt
     5341 ggctcactgc aatctccgcc tcctgggttc aagcgattct cctgcctcag cctcccgagt
     5401 tgttgggatt ccaggcatgc atgaccaggc tcagctaatt tttgtttttt tggtagagac
     5461 ggggtttcac catattggcc aggctggtct ccaactccta atctcaggtg atctacccac
     5521 cttggcctcc caaattgctg ggattacagg cgtgaaccac tgctcccttc cctgtccttc
     5581 tgattttgta ggtaaccacg tgcggaccga gcggccgcag gaacccctag tgatggagtt
     5641 ggccactccc tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg
     5701 acgcccgggc tttgcccggg cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg
     5761 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgtca
     5821 aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     5881 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     5941 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     6001 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt
     6061 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     6121 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcgggctat
     6181 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     6241 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt atggtgcact
     6301 ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc
     6361 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     6421 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga
     6481 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     6541 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     6601 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     6661 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     6721 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     6781 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     6841 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     6901 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     6961 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     7021 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     7081 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     7141 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     7201 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     7261 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     7321 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     7381 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     7441 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     7501 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     7561 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     7621 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     7681 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     7741 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     7801 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
     7861 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     7921 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     7981 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     8041 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     8101 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     8161 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     8221 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     8281 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     8341 ccttttgctc acatgt
//
