LOCUS       Exported                7328 bp DNA     circular SYN 17-JUL-2025
DEFINITION  Exported.
ACCESSION   V014940
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7328)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 7328)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7328
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     26..42
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    56..103
                     /label=FRT
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             121..1983
                     /gene="Rep78"
                     /label=Protein Rep78
                     /note="Protein Rep78 from Adeno-associated virus 2 (isolate
                     Srivastava/1982). Accession#: Q89268"
     CDS             4055..4066
                     /label=WELQut site
                     /note="WELQut protease recognition and cleavage site"
     primer_bind     complement(4539..4555)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(4696..5151)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5178..5282
                     /label=AmpR promoter
     CDS             5283..6140
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      6314..6902
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    7190..7211
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        7226..7256
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7264..7280
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7288..7304
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 atcgttaacg ccccgcgccg gccgctctag aactagtgga tcccccggaa gatcagaagt
       61 tcctattccg aagttcctat tctctagaaa gtataggaac ttctgatctg cgcagccgcc
      121 atgccggggt tttacgagat tgtgattaag gtccccagcg accttgacga gcatctgccc
      181 ggcatttctg acagctttgt gaactgggtg gccgagaagg aatgggagtt gccgccagat
      241 tctgacatgg atctgaatct gattgagcag gcacccctga ccgtggccga gaagctgcag
      301 cgcgactttc tgacggaatg gcgccgtgtg agtaaggccc cggaggccct tttctttgtg
      361 caatttgaga agggagagag ctacttccac atgcacgtgc tcgtggaaac caccggggtg
      421 aaatccatgg ttttgggacg tttcctgagt cagattcgcg aaaaactgat tcagagaatt
      481 taccgcggga tcgagccgac tttgccaaac tggttcgcgg tcacaaagac cagaaatggc
      541 gccggaggcg ggaacaaggt ggtggatgag tgctacatcc ccaattactt gctccccaaa
      601 acccagcctg agctccagtg ggcgtggact aatatggaac agtatttaag cgcctgtttg
      661 aatctcacgg agcgtaaacg gttggtggcg cagcatctga cgcacgtgtc gcagacgcag
      721 gagcagaaca aagagaatca gaatcccaat tctgatgcgc cggtgatcag atcaaaaact
      781 tcagccaggt acatggagct ggtcgggtgg ctcgtggaca aggggattac ctcggagaag
      841 cagtggatcc aggaggacca ggcctcatac atctccttca atgcggcctc caactcgcgg
      901 tcccaaatca aggctgcctt ggacaatgcg ggaaagatta tgagcctgac taaaaccgcc
      961 cccgactacc tggtgggcca gcagcccgtg gaggacattt ccagcaatcg gatttataaa
     1021 attttggaac taaacgggta cgatccccaa tatgcggctt ccgtctttct gggatgggcc
     1081 acgaaaaagt tcggcaagag gaacaccatc tggctgtttg ggcctgcaac taccgggaag
     1141 accaacatcg cggaggccat agcccacact gtgcccttct acgggtgcgt aaactggacc
     1201 aatgagaact ttcccttcaa cgactgtgtc gacaagatgg tgatctggtg ggaggagggg
     1261 aagatgaccg ccaaggtcgt ggagtcggcc aaagccattc tcggaggaag caaggtgcgc
     1321 gtggaccaga aatgcaagtc ctcggcccag atagacccga ctcccgtgat cgtcacctcc
     1381 aacaccaaca tgtgcgccgt gattgacggg aactcaacga ccttcgaaca ccagcagccg
     1441 ttgcaagacc ggatgttcaa atttgaactc acccgccgtc tggatcatga ctttgggaag
     1501 gtcaccaagc aggaagtcaa agactttttc cggtgggcaa aggatcacgt ggttgaggtg
     1561 gagcatgaat tctacgtcaa aaagggtgga gccaagaaaa gacccgcccc cagtgacgca
     1621 gatataagtg agcccaaacg ggtgcgcgag tcagttgcgc agccatcgac gtcagacgcg
     1681 gaagcttcga tcaactacgc agacaggtac caaaacaaat gttctcgtca cgtgggcatg
     1741 aatctgatgc tgtttccctg cagacaatgc gagagaatga atcagaattc aaatatctgc
     1801 ttcactcacg gacagaaaga ctgtttagag tgctttcccg tgtcagaatc tcaacccgtt
     1861 tctgtcgtca aaaaggcgta tcagaaactg tgctacattc atcatatcat gggaaaggtg
     1921 ccagacgctt gcactgcctg cgatctggtc aatgtggatt tggatgactg catctttgaa
     1981 caataaatga tttaaatcag gtatggctgc cgatggttat cttccagatt ggctcgagga
     2041 caacctctct gagggcattc gcgagtggtg ggacttgaaa cctggagccc cgaaacccaa
     2101 agccaaccag caaaagcagg acgacggccg gggtctggtg cttcctggct acaagtacct
     2161 cggacccttc aacggactcg acaaggggga gcccgtcaac gcggcggatg cagcggccct
     2221 cgagcacgac aaggcctacg accagcagct caaagcgggt gacaatccgt acctgcggta
     2281 taaccacgcc gacgccgagt ttcaggagcg tctgcaagaa gatacgtctt ttgggggcaa
     2341 cctcgggcga gcagtcttcc aggccaagaa gagggttctc gaacctcttg gtctggttga
     2401 ggaaggtgct aagacggctc ctggaaagaa acgtccggta gagcagtcgc cacaagagcc
     2461 agactcctcc tcgggcattg gcaagacagg ccagcagccc gctaaaaaga gactcaattt
     2521 tggtcagact ggcgactcag agtcagtccc cgacccacaa cctctcggag aacctccagc
     2581 aacccccgct gctgtgggac ctactacaat ggcttcaggc ggtggcgcac caatggcaga
     2641 caataacgaa ggcgccgacg gagtgggtaa tgcctcagga aattggcatt gcgattccac
     2701 atggctgggc gacagagtca tcaccaccag cacccgaaca tgggccttgc ccacctataa
     2761 caaccacctc tacaagcaaa tctccagtgc ttcaacgggg gccagcaacg acaaccacta
     2821 cttcggctac agcaccccct gggggtattt tgatttcaac agattccact gccatttctc
     2881 accacgtgac tggcagcgac tcatcaacaa caattgggga ttccggccca agagactcaa
     2941 cttcaagctc ttcaacatcc aagtcaagga ggtcacgacg aatgatggcg tcacgaccat
     3001 cgctaataac cttaccagca cggttcaagt cttctcggac tcggagtacc agttgccgta
     3061 cgtcctcggc tctgcgcacc agggctgcct ccctccgttc ccggcggacg tgttcatgat
     3121 tccgcagtac ggctacctaa cgctcaacaa tggcagccag gcagtgggac ggtcatcctt
     3181 ttactgcctg gaatatttcc catcgcagat gctgagaacg ggcaataact ttaccttcag
     3241 ctacaccttc gaggacgtgc ctttccacag cagctacgcg cacagccaga gcctggaccg
     3301 gctgatgaat cctctcatcg accagtacct gtattacctg aacagaactc agaatcagtc
     3361 cggaagtgcc caaaacaagg acttgctgtt tagccggggg tctccagctg gcatgtctgt
     3421 tcagcccaaa aactggctac ctggaccctg ttaccggcag cagcgcgttt ctaaaacaaa
     3481 aacagacaac aacaacagca actttacctg gactggtgct tcaaaatata accttaatgg
     3541 gcgtgaatct ataatcaacc ctggcactgc tatggcctca cacaaagacg acaaagacaa
     3601 gttctttccc atgagcggtg tcatgatttt tggaaaggag agcgccggag cttcaaacac
     3661 tgcattggac aatgtcatga tcacagacga agaggaaatc aaagccacta accccgtggc
     3721 caccgaaaga tttgggactg tggcagtcaa tctccagagc agcagcacag accctgcgac
     3781 cggagatgtg catgttatgg gagccttacc tggaatggtg tggcaagaca gagacgtata
     3841 cctgcagggt cctatttggg ccaaaattcc tcacacggat ggacactttc acccgtctcc
     3901 tctcatgggc ggctttggac ttaagcaccc gcctcctcag atcctcatca aaaacacgcc
     3961 tgttcctgcg aatcctccgg cagagttttc ggctacaaag tttgcttcat tcatcaccca
     4021 gtattccaca ggacaagtga gcgtggagat tgaatgggag ctgcagaaag aaaacagcaa
     4081 acgctggaat cccgaagtgc agtatacatc taactatgca aaatctgcca acgttgattt
     4141 cactgtggac aacaatggac tttatactga gcctcgcccc attggcaccc gttacctcac
     4201 ccgtcccctg taattgtctg ttaatcaata aaccggttaa ttcgtgtcag ttgaactttg
     4261 gtctcatgtc gttattatct tatctggtca ccagatacgt agataagtag catggcgggt
     4321 taatcattaa ctacagcccg ggcgtttaaa cagcgggcgg aggggtggag tcgtgacgtg
     4381 aattacgtca tagggttagg gaggtcctgt attagaggtc acgtgagtgt tttgcgacat
     4441 tttgcgacac catgtggtct cgctgggggg gggggcccga gtgagcacgc agggtctcca
     4501 ttttgaagcg ggaggtttga acgagcgctg gcgcgctcac tggccgtcgt tttacaacgt
     4561 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     4621 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     4681 ctgaatggcg aatggaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt
     4741 gttaaatcag ctcatttttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca
     4801 aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta
     4861 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac
     4921 gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga
     4981 accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa
     5041 aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc
     5101 tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg tcaggtggca
     5161 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     5221 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     5281 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     5341 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     5401 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     5461 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     5521 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     5581 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     5641 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     5701 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     5761 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     5821 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     5881 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     5941 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     6001 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     6061 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     6121 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     6181 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     6241 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     6301 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     6361 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     6421 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     6481 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     6541 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     6601 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     6661 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     6721 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     6781 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     6841 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     6901 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     6961 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     7021 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     7081 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     7141 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     7201 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     7261 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
     7321 gcgccgat
//
