LOCUS       V013603                 7226 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013603
VERSION     V013603
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7226)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7226
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(6..461)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     603..619
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        626..644
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             819..2675
                     /gene="Rep78"
                     /label="Protein Rep78"
                     /note="Protein Rep78 from Adeno-associated virus 2 (isolate
                     Srivastava/1982). Accession#: Q89268"
     primer_bind     complement(4995..5011)
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        complement(5041..5059)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5080..5096)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(5104..5120)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5128..5158)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5173..5194)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5482..6070)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6244..7101)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(7102..7206)
                     /label="AmpR promoter"
ORIGIN
        1 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
       61 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
      121 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
      181 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
      241 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
      301 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
      361 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
      421 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct
      481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
      541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
      601 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tggagctcca
      661 ccgcggtggc ggccgctcta gagtcctgta ttagaggtca cgtgagtgtt ttgcgacatt
      721 ttgcgacacc atgtggtcac gctgggtatt taagcccgag tgagcacgca gggtctccat
      781 tttgaagcgg gaggtttgaa cgcgcagcca ccacggcggg gttttacgag attgtgatta
      841 aggtccccag cgaccttgac gggcatctgc ccggcatttc tgacagcttt gtgaactggg
      901 tggccgagaa ggaatgggag ttgccgccag attctgacat ggatctgaat ctgattgagc
      961 aggcacccct gaccgtggcc gagaagctgc agcgcgactt tctgacggaa tggcgccgtg
     1021 tgagtaaggc cccggaggcc cttttctttg tgcaatttga gaagggagag agctacttcc
     1081 acatgcacgt gctcgtggaa accaccgggg tgaaatccat ggttttggga cgtttcctga
     1141 gtcagattcg cgaaaaactg attcagagaa tttaccgcgg gatcgagccg actttgccaa
     1201 actggttcgc ggtcacaaag accagaaatg gcgccggagg cgggaacaag gtggtggatg
     1261 agtgctacat ccccaattac ttgctcccca aaacccagcc tgagctccag tgggcgtgga
     1321 ctaatatgga acagtattta agcgcctgtt tgaatctcac ggagcgtaaa cggttggtgg
     1381 cgcagcatct gacgcacgtg tcgcagacgc aggagcagaa caaagagaat cagaatccca
     1441 attctgatgc gccggtgatc agatcaaaaa cttcagccag gtacatggag ctggtcgggt
     1501 ggctcgtgga caaggggatt acctcggaga agcagtggat ccaggaggac caggcctcat
     1561 acatctcctt caatgcggcc tccaactcgc ggtcccaaat caaggctgcc ttggacaatg
     1621 cgggaaagat tatgagcctg actaaaaccg cccccgacta cctggtgggc cagcagcccg
     1681 tggaggacat ttccagcaat cggatttata aaattttgga actaaacggg tacgatcccc
     1741 aatatgcggc ttccgtcttt ctgggatggg ccacgaaaaa gttcggcaag aggaacacca
     1801 tctggctgtt tgggcctgca actaccggga agaccaacat cgcggaggcc atagcccaca
     1861 ctgtgccctt ctacgggtgc gtaaactgga ccaatgagaa ctttcccttc aacgactgtg
     1921 tcgacaagat ggtgatctgg tgggaggagg ggaagatgac cgccaaggtc gtggagtcgg
     1981 ccaaagccat tctcggagga agcaaggtgc gcgtggacca gaaatgcaag tcctcggccc
     2041 agatagaccc gactcccgtg atcgtcacct ccaacaccaa catgtgcgcc gtgattgacg
     2101 ggaactcaac gaccttcgaa caccagcagc cgttgcaaga ccggatgttc aaatttgaac
     2161 tcacccgccg tctggatcat gactttggga aggtcaccaa gcaggaagtc aaagactttt
     2221 tccggtgggc aaaggatcac gtggttgagg tggagcatga attctacgtc aaaaagggtg
     2281 gagccaagaa aagacccgcc cccagtgacg cagatataag tgagcccaaa cgggtgcgcg
     2341 agtcagttgc gcagccatcg acgtcagacg cggaagcttc gatcaactac gcagacaggt
     2401 accaaaacaa atgttctcgt cacgtgggca tgaatctgat gctgtttccc tgcagacaat
     2461 gcgagagaat gaatcagaat tcaaatatct gcttcactca cggacagaaa gactgtttag
     2521 agtgctttcc cgtgtcagaa tctcaacccg tttctgtcgt caaaaaggcg tatcagaaac
     2581 tgtgctacat tcatcatatc atgggaaagg tgccagacgc ttgcactgcc tgcgatctgg
     2641 tcaatgtgga tttggatgac tgcatctttg aacaataaat gatttaaata gtcatgtctt
     2701 ttgttgatca ccctccagat tggttggaag aagttggtga aggtcttcgc gagtttttgg
     2761 gccttgaagc gggcccaccg aaaccaaaac ccaatcagca gcatcaagat caagcccgtg
     2821 gtcttgtgct gcctggttat aactatctcg gacccggaaa cggtctcgat cgaggagagc
     2881 ctgtcaacag ggcagacgag gtcgcgcgag agcacgacat ctcgtacaac gagcagcttg
     2941 aggcgggaga caacccctac ctcaagtaca accacgcgga cgccgagttt caggagaagc
     3001 tcgccgacga cacatccttc gggggaaacc tcggaaaggc agtctttcag gccaagaaaa
     3061 gggttctcga accttttggc ctggttgaag agggtgctaa gacggcccct accggaaagc
     3121 ggatagacga ccactttcca aaaagaaaga aggctcggac cgaagaggac tccaagcctt
     3181 ccacctcgtc agacgccgaa gctggaccca gcggatccca gcagctgcaa atcccagccc
     3241 aaccagcctc aagtttggga gctgatacaa tgtctgcggg aggtggcggc ccattgggcg
     3301 acaataacca aggtgccgat ggagtgggca atgcctcggg agattggcat tgcgattcca
     3361 cgtggatggg ggacagagtc gtcaccaagt ccacccgaac ctgggtgctg cccagctaca
     3421 acaaccacca gtaccgagag atcaaaagcg gctccgtcga cggaagcaac gccaacgcct
     3481 actttggata cagcaccccc tgggggtact ttgactttaa ccgcttccac agccactgga
     3541 gcccccgaga ctggcaaaga ctcatcaaca actactgggg cttcagaccc cggtccctca
     3601 gagtcaaaat cttcaacatt caagtcaaag aggtcacggt gcaggactcc accaccacca
     3661 tcgccaacaa cctcacctcc accgtccaag tgtttacgga cgacgactac cagctgccct
     3721 acgtcgtcgg caacgggacc gagggatgcc tgccggcctt ccctccgcag gtctttacgc
     3781 tgccgcagta cggttacgcg acgctgaacc gcgacaacac agaaaatccc accgagagga
     3841 gcagcttctt ctgcctagag tactttccca gcaagatgct gagaacgggc aacaactttg
     3901 agtttaccta caactttgag gaggtgccct tccactccag cttcgctccc agtcagaacc
     3961 tgttcaagct ggccaacccg ctggtggacc agtacttgta ccgcttcgtg agcacaaata
     4021 acactggcgg agtccagttc aacaagaacc tggccgggag atacgccaac acctacaaaa
     4081 actggttccc ggggcccatg ggccgaaccc agggctggaa cctgggctcc ggggtcaacc
     4141 gcgccagtgt cagcgccttc gccacgacca ataggatgga gctcgagggc gcgagttacc
     4201 aggtgccccc gcagccgaac ggcatgacca acaacctcca gggcagcaac acctatgccc
     4261 tggagaacac tatgatcttc aacagccagc cggcgaaccc gggcaccacc gccacgtacc
     4321 tcgagggcaa catgctcatc accagcgaga gcgagacgca gccggtgaac cgcgtggcgt
     4381 acaacgtcgg cgggcagatg gccaccaaca accagagctc caccactgcc cccgcgaccg
     4441 gcacgtacaa cctccaggaa atcgtgcccg gcagcgtgtg gatggagagg gacgtgtacc
     4501 tccaaggacc catctgggcc aagatcccag agacgggggc gcactttcac ccctctccgg
     4561 ccatgggcgg attcggactc aaacacccac cgcccatgat gctcatcaag aacacgcctg
     4621 tgcccggaaa tatcaccagc ttctcggacg tgcccgtcag cagcttcatc acccagtaca
     4681 gcaccgggca ggtcaccgtg gagatggagt gggagctcaa gaaggaaaac tccaagaggt
     4741 ggaacccaga gatccagtac acaaacaact acaacgaccc ccagtttgtg gactttgccc
     4801 cggacagcac cggggaatac agaaccacca gacctatcgg aacccgatac cttacccgac
     4861 ccctttaacc cattcatgtc gcataccctc aataaaccgt gtattcgtgt cagtaaaata
     4921 ctgcctcttg tggtcatttc tagaactaga actagtggat cccccgggct gcaggaattc
     4981 gatatcaagc ttatcgatac cgtcgacctc gagggggggc ccggtaccca gcttttgttc
     5041 cctttagtga gggttaattt cgagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     5101 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     5161 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     5221 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     5281 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     5341 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     5401 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     5461 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     5521 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     5581 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     5641 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     5701 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     5761 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     5821 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     5881 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
     5941 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     6001 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     6061 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     6121 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     6181 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     6241 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     6301 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     6361 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     6421 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     6481 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     6541 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     6601 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     6661 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     6721 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     6781 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     6841 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     6901 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     6961 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     7021 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     7081 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     7141 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     7201 tccgcgcaca tttccccgaa aagtgc
//
