LOCUS       V009666                 7327 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009666
VERSION     V009666
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7327)
  AUTHORS   Grafsky AJ III.
  TITLE     pAAV-RC for AAV Helper-Free System
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7327)
  AUTHORS   Grafsky AJ III.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-APR-2001) Technical Services, Stratagene, 11011 N.
            Torrey Pines Rd., La Jolla, CA 92037, USA
REFERENCE   3  (bases 1 to 7327)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7327)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (16-APR-2001) Technical Services, Stratagene, 11011 N. Torrey Pines
            Rd., La Jolla, CA 92037, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7327
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     36..52
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    66..113
                     /label="FRT"
                     /note="FLP-mediated recombination occurs in the 8-bp core
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             131..1993
                     /gene="Rep78"
                     /label="Protein Rep78"
                     /note="Protein Rep78 from Adeno-associated virus 2 (isolate
                     Srivastava/1982). Accession#: Q89268"
     CDS             2013..4217
                     /gene="VP1"
                     /label="Capsid protein VP1"
                     /note="Capsid protein VP1 from Adeno-associated virus 2
                     (isolate Srivastava/1982). Accession#: P03135"
     primer_bind     complement(4548..4564)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      complement(4705..5160)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5187..5291
                     /label="AmpR promoter"
     CDS             5292..6149
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      6323..6911
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    7199..7220
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        7235..7265
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    7273..7289
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7297..7313
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
ORIGIN
        1 gcgcgccgat atcgttaacg ccccgcgccg gccgctctag aactagtgga tcccccggaa
       61 gatcagaagt tcctattccg aagttcctat tctctagaaa gtataggaac ttctgatctg
      121 cgcagccgcc atgccggggt tttacgagat tgtgattaag gtccccagcg accttgacga
      181 gcatctgccc ggcatttctg acagctttgt gaactgggtg gccgagaagg aatgggagtt
      241 gccgccagat tctgacatgg atctgaatct gattgagcag gcacccctga ccgtggccga
      301 gaagctgcag cgcgactttc tgacggaatg gcgccgtgtg agtaaggccc cggaggccct
      361 tttctttgtg caatttgaga agggagagag ctacttccac atgcacgtgc tcgtggaaac
      421 caccggggtg aaatccatgg ttttgggacg tttcctgagt cagattcgcg aaaaactgat
      481 tcagagaatt taccgcggga tcgagccgac tttgccaaac tggttcgcgg tcacaaagac
      541 cagaaatggc gccggaggcg ggaacaaggt ggtggatgag tgctacatcc ccaattactt
      601 gctccccaaa acccagcctg agctccagtg ggcgtggact aatatggaac agtatttaag
      661 cgcctgtttg aatctcacgg agcgtaaacg gttggtggcg cagcatctga cgcacgtgtc
      721 gcagacgcag gagcagaaca aagagaatca gaatcccaat tctgatgcgc cggtgatcag
      781 atcaaaaact tcagccaggt acatggagct ggtcgggtgg ctcgtggaca aggggattac
      841 ctcggagaag cagtggatcc aggaggacca ggcctcatac atctccttca atgcggcctc
      901 caactcgcgg tcccaaatca aggctgcctt ggacaatgcg ggaaagatta tgagcctgac
      961 taaaaccgcc cccgactacc tggtgggcca gcagcccgtg gaggacattt ccagcaatcg
     1021 gatttataaa attttggaac taaacgggta cgatccccaa tatgcggctt ccgtctttct
     1081 gggatgggcc acgaaaaagt tcggcaagag gaacaccatc tggctgtttg ggcctgcaac
     1141 taccgggaag accaacatcg cggaggccat agcccacact gtgcccttct acgggtgcgt
     1201 aaactggacc aatgagaact ttcccttcaa cgactgtgtc gacaagatgg tgatctggtg
     1261 ggaggagggg aagatgaccg ccaaggtcgt ggagtcggcc aaagccattc tcggaggaag
     1321 caaggtgcgc gtggaccaga aatgcaagtc ctcggcccag atagacccga ctcccgtgat
     1381 cgtcacctcc aacaccaaca tgtgcgccgt gattgacggg aactcaacga ccttcgaaca
     1441 ccagcagccg ttgcaagacc ggatgttcaa atttgaactc acccgccgtc tggatcatga
     1501 ctttgggaag gtcaccaagc aggaagtcaa agactttttc cggtgggcaa aggatcacgt
     1561 ggttgaggtg gagcatgaat tctacgtcaa aaagggtgga gccaagaaaa gacccgcccc
     1621 cagtgacgca gatataagtg agcccaaacg ggtgcgcgag tcagttgcgc agccatcgac
     1681 gtcagacgcg gaagcttcga tcaactacgc agacaggtac caaaacaaat gttctcgtca
     1741 cgtgggcatg aatctgatgc tgtttccctg cagacaatgc gagagaatga atcagaattc
     1801 aaatatctgc ttcactcacg gacagaaaga ctgtttagag tgctttcccg tgtcagaatc
     1861 tcaacccgtt tctgtcgtca aaaaggcgta tcagaaactg tgctacattc atcatatcat
     1921 gggaaaggtg ccagacgctt gcactgcctg cgatctggtc aatgtggatt tggatgactg
     1981 catctttgaa caataaatga tttaaatcag gtatggctgc cgatggttat cttccagatt
     2041 ggctcgagga cactctctct gaaggaataa gacagtggtg gaagctcaaa cctggcccac
     2101 caccaccaaa gcccgcagag cggcataagg acgacagcag gggtcttgtg cttcctgggt
     2161 acaagtacct cggacccttc aacggactcg acaagggaga gccggtcaac gaggcagacg
     2221 ccgcggccct cgagcacgac aaagcctacg accggcagct cgacagcgga gacaacccgt
     2281 acctcaagta caaccacgcc gacgcggagt ttcaggagcg ccttaaagaa gatacgtctt
     2341 ttgggggcaa cctcggacga gcagtcttcc aggcgaaaaa gagggttctt gaacctctgg
     2401 gcctggttga ggaacctgtt aagacggctc cgggaaaaaa gaggccggta gagcactctc
     2461 ctgtggagcc agactcctcc tcgggaaccg gaaaggcggg ccagcagcct gcaagaaaaa
     2521 gattgaattt tggtcagact ggagacgcag actcagtacc tgacccccag cctctcggac
     2581 agccaccagc agccccctct ggtctgggaa ctaatacgat ggctacaggc agtggcgcac
     2641 caatggcaga caataacgag ggcgccgacg gagtgggtaa ttcctcggga aattggcatt
     2701 gcgattccac atggatgggc gacagagtca tcaccaccag cacccgaacc tgggccctgc
     2761 ccacctacaa caaccacctc tacaaacaaa tttccagcca atcaggagcc tcgaacgaca
     2821 atcactactt tggctacagc accccttggg ggtattttga cttcaacaga ttccactgcc
     2881 acttttcacc acgtgactgg caaagactca tcaacaacaa ctggggattc cgacccaaga
     2941 gactcaactt caagctcttt aacattcaag tcaaagaggt cacgcagaat gacggtacga
     3001 cgacgattgc caataacctt accagcacgg ttcaggtgtt tactgactcg gagtaccagc
     3061 tcccgtacgt cctcggctcg gcgcatcaag gatgcctccc gccgttccca gcagacgtct
     3121 tcatggtgcc acagtatgga tacctcaccc tgaacaacgg gagtcaggca gtaggacgct
     3181 cttcatttta ctgcctggag tactttcctt ctcagatgct gcgtaccgga aacaacttta
     3241 ccttcagcta cacttttgag gacgttcctt tccacagcag ctacgctcac agccagagtc
     3301 tggaccgtct catgaatcct ctcatcgacc agtacctgta ttacttgagc agaacaaaca
     3361 ctccaagtgg aaccaccacg cagtcaaggc ttcagttttc tcaggccgga gcgagtgaca
     3421 ttcgggacca gtctaggaac tggcttcctg gaccctgtta ccgccagcag cgagtatcaa
     3481 agacatctgc ggataacaac aacagtgaat actcgtggac tggagctacc aagtaccacc
     3541 tcaatggcag agactctctg gtgaatccgg gcccggccat ggcaagccac aaggacgatg
     3601 aagaaaagtt ttttcctcag agcggggttc tcatctttgg gaagcaaggc tcagagaaaa
     3661 caaatgtgga cattgaaaag gtcatgatta cagacgaaga ggaaatcagg acaaccaatc
     3721 ccgtggctac ggagcagtat ggttctgtat ctaccaacct ccagagaggc aacagacaag
     3781 cagctaccgc agatgtcaac acacaaggcg ttcttccagg catggtctgg caggacagag
     3841 atgtgtacct tcaggggccc atctgggcaa agattccaca cacggacgga cattttcacc
     3901 cctctcccct catgggtgga ttcggactta aacaccctcc tccacagatt ctcatcaaga
     3961 acaccccggt acctgcgaat ccttcgacca ccttcagtgc ggcaaagttt gcttccttca
     4021 tcacacagta ctccacggga caggtcagcg tggagatcga gtgggagctg cagaaggaaa
     4081 acagcaaacg ctggaatccc gaaattcagt acacttccaa ctacaacaag tctgttaatg
     4141 tggactttac tgtggacact aatggcgtgt attcagagcc tcgccccatt ggcaccagat
     4201 acctgactcg taatctgtaa ttgcttgtta atcaataaac cgtttaattc gtttcagttg
     4261 aactttggtc tctgcgtatt tctttcttat ctagtttcca tggctacgta gataagtagc
     4321 atggcgggtt aatcattaac tacagcccgg gcgtttaaac agcgggcgga ggggtggagt
     4381 cgtgacgtga attacgtcat agggttaggg aggtcctgta ttagaggtca cgtgagtgtt
     4441 ttgcgacatt ttgcgacacc atgtggtctc gctggggggg ggggcccgag tgagcacgca
     4501 gggtctccat tttgaagcgg gaggtttgaa cgagcgctgg cgcgctcact ggccgtcgtt
     4561 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     4621 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     4681 ttgcgcagcc tgaatggcga atggaaattg taagcgttaa tattttgtta aaattcgcgt
     4741 taaatttttg ttaaatcagc tcattttttt aaccaatagg ccgaaatcgg caaaatccct
     4801 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt
     4861 ccactattaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
     4921 gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac
     4981 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg
     5041 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag
     5101 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt
     5161 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     5221 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     5281 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     5341 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     5401 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     5461 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     5521 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     5581 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     5641 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     5701 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     5761 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     5821 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     5881 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     5941 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     6001 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     6061 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     6121 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     6181 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     6241 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     6301 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     6361 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     6421 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     6481 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     6541 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     6601 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     6661 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     6721 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     6781 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     6841 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     6901 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     6961 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     7021 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     7081 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     7141 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     7201 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     7261 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     7321 acgccaa
//
