LOCUS       62056_2460        9832 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9832)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9832
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(689..722)
                     /label=lox2272
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(773..806)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(812..931)
                     /codon_start=1
                     /label=hPEST
                     /note="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     CDS             complement(935..982)
                     /codon_start=1
                     /label=hCL1
                     /note="non-ORF yeast peptide conferring ubiquitin-dependent
                     degradation"
                     /translation="ACKNWFSSLSHFVIHL"
     CDS             complement(989..2635)
                     /codon_start=1
                     /label=luciferase
                     /note="firefly luciferase"
                     /translation="EDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDAH
                     IEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAPA
                     NDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQSM
                     YTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHAR
                     DPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYKI
                     QSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGYG
                     LTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSG
                     YVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILL
                     QHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVF
                     VDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     CDS             complement(2645..2665)
                     /codon_start=1
                     /label=TEV site
                     /note="tobacco etch virus (TEV) protease recognition and 
                     cleavage site"
                     /translation="ENLYFQG"
     CDS             complement(2690..2734)
                     /codon_start=1
                     /label=AviTag(TM)
                     /note="peptide tag that allows for enzymatic biotinylation"
                     /translation="GLNDIFEAQKIEWHE"
     CDS             complement(2756..2821)
                     /codon_start=1
                     /label=3xFLAG
                     /note="three tandem FLAG(R) epitope tags, followed by an 
                     enterokinase cleavage site"
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             complement(3548..3571)
                     /codon_start=1
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
                     /translation="DYKDDDDK"
     promoter        complement(3618..3636)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        complement(3642..3660)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     intron          complement(3705..3837)
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     protein_bind    3902..3935
                     /label=lox2272
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    3986..4019
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        4029..4528
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             4555..5271
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    5959..6547
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     polyA_signal    6579..7055
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     repeat_region   7095..7235
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      7310..7765
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        8047..8151
                     /label=AmpR promoter
     CDS             8152..9009
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      9183..9771
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtcggatt accgaggctg gtcacgtcgt cgcaggtgat
      181 aggccggggg ccctggtctc tgccggctgt gagttgcgca gcggccaagc accattcccc
      241 cgcgccgcag tggtacgcgc cactccgggg ctgcacgagc gggccaccgc cgtgccaggt
      301 gaatggaagt cccgcaggcc ggaagtggac gagcctactc gcccgggcgc gggggggcgc
      361 aagagcgcgc agcatcttca ttgaggaacc cgggcggcga acatggagtt ccatgtgcgt
      421 cttatgtaaa gagagcgacg ggcgtctcca ccaattgacg agcgtagctc tcaggttccg
      481 ccccgccagt atgcaaatga ggtggcactc cgaccaatgg cgcgcgcagg ggcgggctca
      541 gcgcgcgcgg tcacgttttc cactatgtga cagcggaggg cgacgcggcg gcagcggcgc
      601 tactgggact agcggctccg ggcggctgcg gcgcaggccg agcgcaccaa gtgacgggcc
      661 gagcaaggga tctagagtcg actccggaat aacttcgtat aggatacttt atacgaagtt
      721 atgcagaatg gtagctggat tgtagctgct attagcaata tgaaacctct taataacttc
      781 gtatagcata cattatacga agttatggtt agacgttgat cctggcgctg gcgcaagcag
      841 cagggtgtct atccatgccg ctctcctggg cgcagctcat gggcagggtg ccggcggcct
      901 gctcctccac ctcgggaggg aagccgtggc tgttaaggtg gatcacaaag tggcttaagc
      961 tactgaacca gttcttgcaa gcagaattca cggcgatctt gccgcccttc ttggccttaa
     1021 tgagaatctc gcggatcttg cgggcgtcca acttgccggt cagtccttta ggcacctcgt
     1081 ccacgaacac aacaccaccg cgcagcttct tggcggttgt aacctggctg gccacatagt
     1141 ccacgatctc cttctcggtc atggttttac cgtgttccag cacgacgact gcggcgggca
     1201 gctcgccggc atcgtcgtcg ggcaggccgg cgaccccggc gtcgaagatg ttggggtgtt
     1261 gcagcaggat gctctccagt tcggctgggg ctacctggta gcccttgtat ttgatcaggc
     1321 tcttcagccg gtccacgatg aagaagtgct cgtcctcgtc ccagtaggcg atgtcgccgc
     1381 tgtgcagcca gccgtccttg tcgatgagag cgtttgtagc ctcggggttg ttaacgtagc
     1441 cgctcatgat catggggcca cggacgcaca gctcgccgcg ctggttcaca cccagtgtct
     1501 taccggtgtc caagtccacc accttagcct cgaagaaggg caccaccttg cctactgcgc
     1561 caggcttgtc gtccccttcg ggggtgatca gaatggcgct ggttgtttct gtcaggccgt
     1621 agccctggcg gatgcctggt aggtggaagc gtttggccac ggcctcacct acctccttgc
     1681 tgagcggcgc cccgccgctg gcgatctcgt gcaagttgct taggtcgtac ttgtcgatga
     1741 gagtgctctt agcgaagaag ctaaatagtg tgggcaccag cagggcagat tgaatcttat
     1801 agtcttgcaa gctgcgcaag aatagctcct cctcgaagcg gtacatgagc acgacccgaa
     1861 agccgcagat caagtagccc agcgtggtga acatgccgaa gccgtggtga aatggcacca
     1921 cgctgaggat agcggtgtcg gggatgatct ggttgccgaa gatggggtcg cgggcatgac
     1981 tgaatcggac acaagcggtg cggtgcggta gggctacgcc cttgggcaat ccggtactgc
     2041 cactactgtt catgatcagg gcgatggttt tgtcccggtc gaagctctcg ggcacgaagt
     2101 cgtactcgtt gaagccgggt ggcaaatggg aagtcacgaa ggtgtacatg ctttggaagc
     2161 cctggtagtc ggtcttgcta tccatgatga tgatcttttg tatgatcggt agcttctttt
     2221 gcacgttgag gatcttttgc agccctttct tgctcacgaa tacgacggtg ggctggctga
     2281 tgcccatgct gttcagcagc tcgcgctcgt tgtagatgtc gttagctggg gccacagcca
     2341 caccgatgaa cagggcaccc aacacgggca tgaagaactg caagctattc tcgctgcaca
     2401 ccacgatccg atggtttgta ttcagcccat agcgcttcat agcttctgcc agccgaacgc
     2461 tcatctcgaa gtactcggcg taggtaatgt ccacctcgat atgtgcgtcg gtaaaggcga
     2521 tggtgccggg caccagggcg tagcgcttca tggctttgtg cagctgctcg ccggcggtcc
     2581 cgtcttcgag tgggtagaat ggcgctgggc ccttcttaat gtttttggca tcttcacctg
     2641 cagggccctg aaaatacagg ttttcggatc ctgaaccgcc tgccgatccc tcatgccact
     2701 ctattttctg agcttcgaaa atatcgttca ggccgctagc tgcggccgct ccacccttat
     2761 cgtcatcgtc cttgtagtcg atgtcatgat ctttataatc accgtcatgg tctttgtagt
     2821 cctgcaaagg aagatagata gataagatct ccacacgcct cttcccccaa ccagccgata
     2881 aaactgacca gggacatcac accagagatt ttaaagaaaa tattaccgta tgctgggtgg
     2941 tggctttggg acaaaggact tgcctggagt gcctgagacc catagtatgg cctccatact
     3001 tcccctcaaa ataaaagtca gagctggaga atcagctcaa tggtgaagag aagcacccac
     3061 acggctgctt acacccatct gtaactcaca gttccagggg atccaatgcc ctcttctgtc
     3121 ccctatggtc accaaacatg caagtggtgc accgacatac atacaggcaa aacacccata
     3181 tactttaaag taaaaattta aaaaaggaaa agtcatgtta aaactgaaac ataagagctt
     3241 cagctattag gagacttgaa cactgagaga cagaaatgaa aaggaatatt ctgtgatcga
     3301 atgcatgtgt gtgagcaggc tcctccgagg tcttactgga ctgtctgtta agtgcatcag
     3361 atacaaaaat gcaaagatga cgtggtatcg ttatttggaa ggacaaagta caataaatac
     3421 catctacagg attaagaagt actgccatcc tgcaagtcct ggtacttgct gcttgaagac
     3481 gggcgacaaa ctatggaggg agccacccgt gtcctgtgtg cgagggcctg gcctgaggct
     3541 gacctacctt gtcatcgtca tccttgtagt ccatggtggc ctctccttcg agtgcaaaaa
     3601 gaacaagtag cttgtattct atagtgtcac ctaaatgcta gcctatagtg agtcgtatta
     3661 agtactctag ccttaagagc tgtaattgaa ctgggagtgg acacctgtgg agagaaaggc
     3721 aaagtggatg tcagtaagac caataggtgc ctatcagaaa cgcaagagtc ttctctgtct
     3781 cgacaagccc agtttctatt ggtctcctta aacctgtctt gtaaccttga tacttacctg
     3841 cccagtgcct cacgaccaac tttcgctacc ttaggaccgt tatagttact gcagcgctag
     3901 cataacttcg tataaagtat cctatacgaa gttatttgcc ttaacccaga aattatcact
     3961 gttattcttt agaatggtgc aaagaataac ttcgtataat gtatgctata cgaagttatg
     4021 aattcgatgg gtaggggagg cgcttttccc aaggcagtct ggagcatgcg ctttagcagc
     4081 cccgctgggc acttggcgct acacaagtgg cctctggcct cgcacacatt ccacatccac
     4141 cggtaggcgc caaccggctc cgttctttgg tggccccttc gcgccacctt ctactcctcc
     4201 cctagtcagg aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga
     4261 agtagcacgt ctcactagtc tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt
     4321 aggcctttgg ggcagcggcc aatagcagct ttgctccttc gctttctggg ctcagaggct
     4381 gggaaggggt gggtccgggg gcgggctcag gggcgggctc aggggcgggg cgggcgcccg
     4441 aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt
     4501 ctcctcttcc tcatctccgg gcctttcgac ctgcagccca agcttaccgc caccatggtg
     4561 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     4621 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
     4681 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     4741 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
     4801 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
     4861 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     4921 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     4981 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc
     5041 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
     5101 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     5161 agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     5221 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa ggaatttggc
     5281 agcggccagt gcaccaacta tgccctgctg aaactggctg gcgatgtgga aagcaacccc
     5341 ggacccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg
     5401 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat
     5461 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg
     5521 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg
     5581 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg
     5641 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc
     5701 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt
     5761 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc
     5821 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc
     5881 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt
     5941 gcctgaaagc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     6001 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     6061 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     6121 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     6181 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     6241 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     6301 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     6361 tcctttcctt ggctgctcgc ctatgttgcc acctggattc tgcgcgggac gtccttctgc
     6421 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     6481 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     6541 tccccgcatc gataccgagc gctgctcgag agatctacgg gtggcatccc tgtgacccct
     6601 ccccagtgcc tctcctggcc ctggaagttg ccactccagt gcccaccagc cttgtcctaa
     6661 taaaattaag ttgcatcatt ttgtctgact aggtgtcctt ctataatatt atggggtgga
     6721 ggggggtggt atggagcaag gggcaagttg ggaagacaac ctgtagggcc tgcggggtct
     6781 attgggaacc aagctggagt gcagtggcac aatcttggct cactgcaatc tccgcctcct
     6841 gggttcaagc gattctcctg cctcagcctc ccgagttgtt gggattccag gcatgcatga
     6901 ccaggctcag ctaatttttg tttttttggt agagacgggg tttcaccata ttggccaggc
     6961 tggtctccaa ctcctaatct caggtgatct acccaccttg gcctcccaaa ttgctgggat
     7021 tacaggcgtg aaccactgct cccttccctg tccttctgat tttgtaggta accacgtgcg
     7081 gaccgagcgg ccgcaggaac ccctagtgat ggagttggcc actccctctc tgcgcgctcg
     7141 ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc
     7201 ctcagtgagc gagcgagcgc gcagctgcct gcaggggcgc ctgatgcggt attttctcct
     7261 tacgcatctg tgcggtattt cacaccgcat acgtcaaagc aaccatagta cgcgccctgt
     7321 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     7381 agcgccttag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     7441 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     7501 cacctcgacc ccaaaaaact tgatttgggt gatggttcac gtagtgggcc atcgccctga
     7561 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     7621 caaactggaa caacactcaa ctctatctcg ggctattctt ttgatttata agggattttg
     7681 ccgatttcgg tctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     7741 aacaaaatat taacgtttac aattttatgg tgcactctca gtacaatctg ctctgatgcc
     7801 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
     7861 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     7921 aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt
     7981 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     8041 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     8101 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     8161 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     8221 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     8281 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     8341 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     8401 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     8461 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     8521 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     8581 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     8641 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     8701 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     8761 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     8821 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     8881 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     8941 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     9001 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     9061 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     9121 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     9181 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     9241 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     9301 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     9361 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     9421 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     9481 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     9541 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     9601 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     9661 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     9721 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     9781 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gt
//
