LOCUS       40924_3169        5230 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pAAV-ISceI.AO3, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5230)
  AUTHORS   Inagaki K, Lewis SM, Wu X, Ma C, Munroe DJ, Fuess S, Storm TA, Kay 
            MA, Nakai H.
  TITLE     DNA palindromes with a modest arm length of greater, similar 20 base
            pairs are a significant target for recombinant adeno-associated 
            virus vector integration in the liver, muscles, and heart in mice
  JOURNAL   J. Virol. 81 (20), 11290-11303 (2007)
  PUBMED    17686840
REFERENCE   2  (bases 1 to 5230)
  AUTHORS   Nakai H, Inagaki K.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2007) Department of Molecular Genetics and 
            Biochemistry, University of Pittsburgh School of Medicine, W1244 BST
            200 Lothrop Street, Pittsburgh, PA 15261, USA
REFERENCE   3  (bases 1 to 5230)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5230)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Virol.";
            date: "2007"; volume: "81"; issue: "20"; pages: "11290-11303"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (07-JUL-2007) Department of Molecular Genetics and Biochemistry, 
            University of Pittsburgh School of Medicine, W1244 BST 200 Lothrop 
            Street, Pittsburgh, PA 15261, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5230
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             640..660
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     CDS             664..684
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     CDS             688..708
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     misc_feature    741..758
                     /label=I-Sce I recognition site
                     /note="I-Sce I recognition site"
     regulatory      764..817
                     /note="transposon Tn3 promoter from GenBank Accession
                     Number V00613"
                     /regulatory_class="promoter"
     CDS             818..1675
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1849..2437
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     repeat_region   2512..2718
                     /label=murine leukemia virus (MLV) LTR from NC_001501
                     /note="murine leukemia virus (MLV) LTR from NC_001501"
     repeat_region   2780..2920
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      2995..3450
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        complement(3862..3890)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     rep_origin      4440..5028
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     repeat_region   5090..5230
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
ORIGIN
        1 ggaggggtgg agtcgtgacg tgggcaagct gaccctgaag ttcatctgca ccaccggcaa
       61 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag
      121 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
      181 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
      241 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
      301 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
      361 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
      421 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
      481 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa
      541 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
      601 catggacgag ctgtacaagt ccggactcat cgagctgatc caaaaaagaa gagaaaggta
      661 gatccaaaaa agaagagaaa ggtagatcca aaaaagaaga gaaaggtagg atcgatccac
      721 cggatctaga taactgatca tagggataac agggtaatgg atcctcatga gacaataacc
      781 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
      841 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
      901 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
      961 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     1021 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     1081 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     1141 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     1201 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     1261 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     1321 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     1381 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     1441 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     1501 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     1561 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     1621 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     1681 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     1741 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     1801 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     1861 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     1921 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     1981 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     2041 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     2101 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     2161 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     2221 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     2281 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     2341 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     2401 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     2461 acggttcctg gccttttgct ggccttttgc tcacatgtac aagcttatgc attaccacag
     2521 atatcctgtt tggcccatat tcagctgttc catctgttct tggccctgag ccggggcagg
     2581 aactgcttac cacagatatc ctgtttggcc catattcagc tgttccatct gttcctgacc
     2641 ttgatctgaa cttctctatt ctcagttatg tatttttcca tgccttgcaa aatggcgtta
     2701 cttaagctag cttgccaatg catgtttaaa catggtgcgg acgtagataa gtagcatggc
     2761 gggttaatca ttaactacaa ggaaccccta gtgatggagt tggccactcc ctctctgcgc
     2821 gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg
     2881 gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt
     2941 ctccttacgc atctgtgcgg tatttcacac cgcatacgtc aaagcaacca tagtacgcgc
     3001 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     3061 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     3121 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     3181 tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc
     3241 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     3301 tgttccaaac tggaacaaca ctcaacccta tctcgggcta ttcttttgat ttataaggga
     3361 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     3421 attttaacaa aatattaacg tttacaattt tatggtgcac tctcagtaca atctgctctg
     3481 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     3541 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
     3601 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc
     3661 tatttttata ggttaatgtc atgataataa tggtttctta gctcatggcg ttatcccgca
     3721 agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc
     3781 cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat
     3841 ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat
     3901 tacaacttat atcgtatggg gctgacttca ggtgctacat ttgaagagat aaattgcact
     3961 gaaatctaga aatgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     4021 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     4081 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     4141 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     4201 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     4261 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     4321 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     4381 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     4441 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     4501 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     4561 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     4621 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     4681 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     4741 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     4801 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     4861 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     4921 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     4981 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     5041 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtc ctgcaggcag
     5101 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt
     5161 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
     5221 aggggttcct
//
