LOCUS       pAAV-Gas6(mouse)        6676 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V018636
VERSION     V018636
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     repeat_region   1..141
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     enhancer        221..524
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        525..727
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     intron          876..1254
                     /note="fusion between human cytomegalovirus intron A and
                     human β-globin intron 2"
                     /label="chimeric intron"
     CDS             1338..3359
                     /label="Gas6(NM_019521)"
                     /note="Gas6(NM_019521)"
                     /gene="Gas6"
     polyA_signal    3423..3899
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     repeat_region   3939..4079
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     rep_origin      4154..4609
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4891..4995
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             4996..5856
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      6027..6615
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtggagc tagttattaa tagtaatcaa
      181 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
      241 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
      301 ttcccatagt aacgtcaata gggactttcc attgacgtca atgggtggag tatttacggt
      361 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
      421 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
      481 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
      541 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
      601 ttgacgtcaa tgggagtttg ttttgcacca aaatcaacgg gactttccaa aatgtcgtaa
      661 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
      721 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
      781 ccatagaaga caccgggacc gatccagcct ccgcggattc gaatcccggc cgggaacggt
      841 gcattggaac gcggattccc cgtgccaaga gtgacgtaag taccgcctat agagtctata
      901 ggcccacaaa aaatgctttc ttcttttaat atactttttt gtttatctta tttctaatac
      961 tttccctaat ctctttcttt cagggcaata atgatacaat gtatcatgcc tctttgcacc
     1021 attctaaaga ataacagtga taatttctgg gttaaggcaa tagcaatatt tctgcatata
     1081 aatatttctg catataaatt gtaactgatg taagaggttt catattgcta atagcagcta
     1141 caatccagct accattctgc ttttatttta tggttgggat aaggctggat tattctgagt
     1201 ccaagctagg cccttttgct aatcatgttc atacctctta tcttcctccc acagctcctg
     1261 ggcaacgtgc tggtctgtgt gctggcccat cactttggca aagaattggg attcgaacat
     1321 cgattgaatt cgccaccatg ccgccaccgc ccgggcccgc cgccgccctg ggcactgcgc
     1381 ttctgctgct cctgctggct tccgagtctt ctcacactgt gctgttgcgg gcgcgtgagg
     1441 cggcgcagtt tctgcggccc aggcagcgcc gcgcctacca agtcttcgag gaggccaagc
     1501 agggccacct ggaacgggag tgcgtggagg aggtgtgcag caaagaggag gccagagagg
     1561 tgttcgagaa cgaccccgag acggagtatt tctatccacg atatcaagag tgcatgagaa
     1621 aatatggcag gcctgaagaa aaaaacccag atttcgccaa atgtgttcag aacttgcctg
     1681 accagtgcac cccaaaccct tgtgataaga agggtactca tatctgccaa gacctcatgg
     1741 gcaacttctt ctgcgtgtgc acagatggct ggggaggccg gctctgtgac aaagatgtca
     1801 atgagtgtgt ccagaagaat gggggctgca gccaggtctg ccacaacaaa ccaggaagct
     1861 tccaatgtgc ctgccatagt ggcttctcgc ttgcatcaga cggccagacc tgccaagata
     1921 tcgatgaatg cacagactca gacacctgtg gggacgcgcg atgcaagaac ttgccaggct
     1981 cctactcttg cctctgcgat gagggatata catacagctc caaggagaag acctgccaag
     2041 atgtggacga gtgccagcag gatcgctgtg agcagacctg tgtcaactcc ccaggcagct
     2101 atacctgcca ctgtgatggg cgagggggcc taaaactatc cccagacatg gatacttgtg
     2161 aggacatctt accatgtgtg cccttcagca tggccaagag cgtgaagtcc ttgtacctgg
     2221 gccgcatgtt cagcgggacc cccgtgatta gactacgctt caagaggctt cagcctacca
     2281 ggctgctggc tgaatttgac ttccgcactt ttgaccctga aggagtcctc ttcttcgctg
     2341 gaggccgttc agacagcacc tggattgtcc tgggcctaag agctgggcgg cttgagctgc
     2401 agcttcggta caatggcgtt gggcgcatca ccagcagcgg gccaaccatc aaccacggca
     2461 tgtggcaaac tatctccgtg gaagagctgg aacgtaacct tgtcatcaag gtcaacaaag
     2521 atgctgtaat gaagatcgcg gtagctgggg agctgtttca gctggagagg ggcctctatc
     2581 acctgaatct caccgtgggc ggcattccct tcaaggagag tgagctcgtc cagccgatta
     2641 accctcgcct ggatgggtgc atgaggagtt ggaactggct gaacggggaa gacagcgcca
     2701 tccaggagac agtcaaggca aacacaaaaa tgcagtgctt ctctgtgaca gaaaggggct
     2761 ccttcttccc ggggaatgga tttgctacct acaggctcaa ctacacccga acatcgctgg
     2821 atgtcggcac ggaaaccacc tgggaagtta aagttgtggc tcggatccgc cctgccacgg
     2881 acacgggggt gctgctggcg ctggtggggg acgacgatgt cgtccccatc tctgtggccc
     2941 tagtcgacta ccactctaca aagaagctca agaagcagtt ggtggtcctg gcagttgagg
     3001 atgttgccct ggcactgatg gaaatcaagg tgtgcgacag ccaggaacac acggtcactg
     3061 tctccctgcg ggagggtgag gccaccctag aagtggatgg cacaaagggc cagagtgaag
     3121 tgagcactgc ccagctgcag gagcgactgg acacacttaa gacacatctg caaggctctg
     3181 tgcacaccta tgttggaggc ctgccagaag tatcggtgat ttctgcaccc gtcactgcgt
     3241 tctaccgcgg atgcatgact ctggaggtaa acgggaaaat cctggacctg gatacggcct
     3301 cgtacaagca cagtgacatc acctcccact cctgcccgcc tgtggagcat gccaccccct
     3361 agggatcctc tagagtcgac ctgcagaagc ttgcctcgag cagcgctgct cgagagatct
     3421 acgggtggca tccctgtgac ccctccccag tgcctctcct ggccctggaa gttgccactc
     3481 cagtgcccac cagccttgtc ctaataaaat taagttgcat cattttgtct gactaggtgt
     3541 ccttctataa tattatgggg tggagggggg tggtatggag caaggggcaa gttgggaaga
     3601 caacctgtag ggcctgcggg gtctattggg aaccaagctg gagtgcagtg gcacaatctt
     3661 ggctcactgc aatctccgcc tcctgggttc aagcgattct cctgcctcag cctcccgagt
     3721 tgttgggatt ccaggcatgc atgaccaggc tcagctaatt tttgtttttt tggtagagac
     3781 ggggtttcac catattggcc aggctggtct ccaactccta atctcaggtg atctacccac
     3841 cttggcctcc caaattgctg ggattacagg cgtgaaccac tgctcccttc cctgtccttc
     3901 tgattttgta ggtaaccacg tgcggaccga gcggccgcag gaacccctag tgatggagtt
     3961 ggccactccc tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg
     4021 acgcccgggc tttgcccggg cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg
     4081 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgtca
     4141 aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     4201 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     4261 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     4321 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt
     4381 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     4441 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcgggctat
     4501 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     4561 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt atggtgcact
     4621 ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc
     4681 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     4741 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga
     4801 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     4861 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     4921 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     4981 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     5041 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     5101 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     5161 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     5221 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     5281 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     5341 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     5401 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     5461 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     5521 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     5581 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     5641 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     5701 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     5761 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     5821 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     5881 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     5941 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     6001 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     6061 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     6121 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
     6181 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     6241 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     6301 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     6361 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     6421 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     6481 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     6541 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     6601 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     6661 ccttttgctc acatgt
//
