LOCUS       V009672                 6772 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009672
VERSION     V009672
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6772)
  AUTHORS   Nakai H, Wu X, Fuess S, Storm TA, Munroe D, Montini E, Burgess SM,
            Grompe M, Kay MA.
  TITLE     Large-scale molecular characterization of adeno-associated virus
            vector integration in mouse liver
  JOURNAL   J. Virol. 79 (6), 3606-3614 (2005)
   PUBMED   15731255
REFERENCE   2  (bases 1 to 6772)
  AUTHORS   Nakai H, Wu X, Fuess S, Storm TA, Munroe D, Montini E, Burgess SM,
            Grompe M, Kay MA.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JAN-2007) Department of Molecular Genetics and
            Biochemistry, University of Pittsburgh School of Medicine, W1244 BST
            200 Lothrop Street, Pittsburgh, PA 15261, USA
REFERENCE   3  (bases 1 to 6772)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6772)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Virol.";
            date: "2005"; volume: "79"; issue: "6"; pages: "3606-3614"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (01-JAN-2007) Department of Molecular Genetics and Biochemistry,
            University of Pittsburgh School of Medicine, W1244 BST 200 Lothrop
            Street, Pittsburgh, PA 15261, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6772
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          233..1175
                     /label="EF-1-alpha intron A"
                     /note="intron upstream of the start codon of human
                     EF-1-alpha"
     CDS             1243..2499
                     /gene="FAH"
                     /label="Fumarylacetoacetase"
                     /note="Fumarylacetoacetase from Homo sapiens. Accession#:
                     P16930"
     regulatory      2625..2834
                     /label="poly A signal from Homo sapiens beta globin"
                     /note="poly A signal from Homo sapiens beta globin"
                     /regulatory_class="polyA_signal_sequence"
     promoter        2878..2982
                     /label="AmpR promoter"
     CDS             2983..3840
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      4014..4602
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     repeat_region   4734..4863
                     /note="adeno-associated virus serotype 2 inverted terminal
                     repeat"
     repeat_region   6643..6772
                     /note="adeno-associated virus serotype 2 inverted terminal
                     repeat"
ORIGIN
        1 ggaggggtgg agtcgtgacg tgaattacgt catagggtta gggaggtcgt ccgcacggcc
       61 gctctagaac tagaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag
      121 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc
      181 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc
      241 cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat
      301 tacttccacg cccctggctg cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg
      361 ggtgggagag ttcgaggcct tgcgcttaag gagccccttc gcctcgtgct tgagttgagg
      421 cctggcctgg gcgctggggc cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg
      481 ctgctttcga taagtctcta gccatttaaa atttttgatg acctgctgcg acgctttttt
      541 tctggcaaga tagtcttgta aatgcgggcc aagatctgca cactggtatt tcggtttttg
      601 gggccgcggg cggcgacggg gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc
      661 tgcgagcgcg gccaccgaga atcggacggg ggtagtctca agctggccgg cctgctctgg
      721 tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg
      781 caccagttgc gtgagcggaa agatggccgc ttcccggccc tgctgcaggg agctcaaaat
      841 ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct
      901 ttccgtcctc agccgtcgct tcatgtgact ccacggagta ccgggcgccg tccaggcacc
      961 tcgattagtt ctcgagcttt tggagtacgt cgtctttagg ttggggggag gggttttatg
     1021 cgatggagtt tccccacact gagtgggtgg agactgaagt taggccagct tggcacttga
     1081 tgtaattctc cttggaattt gccctttttg agtttggatc ttggttcatt ctcaagcctc
     1141 agacagtggt tcaaagtttt tttcttccat ttcaggtgtc gtgaaaacta cccctaaaag
     1201 ccaaagaatt cgcccttgaa ggggatccgg gtgctcttca gcatgtcctt catcccggtg
     1261 gccgaggatt ccgacttccc catccacaac ctgccctacg gcgtcttctc gaccagaggc
     1321 gacccaagac cgaggatagg tgtggccatt ggcgaccaga tcctggacct cagcatcatc
     1381 aagcacctct ttactggtcc tgtcctctcc aaacaccagg atgtcttcaa tcagcctaca
     1441 ctcaacagct tcatgggcct gggtcaggct gcctggaagg aggcgagagt gttcttgcag
     1501 aacttgctgt ctgtgagcca agccaggctc agagatgaca ccgaacttcg gaagtgtgca
     1561 ttcatctccc aggcttctgc cacgatgcac cttccagcca ccataggaga ctacacagac
     1621 ttctattcct ctcggcagca tgctaccaac gtcggaatca tgttcaggga caaggagaat
     1681 gcgttgatgc caaattggct gcacttacca gtgggctacc atggccgtgc ctcctctgtc
     1741 gtggtgtctg gcaccccaat ccgaaggccc atgggacaga tgaaacctga tgactctaag
     1801 cctcccgtat atggtgcctg caagctcttg gacatggagc tggaaatggc tttttttgta
     1861 ggccctggaa acagattggg agagccgatc cccatttcca aggcccatga gcacattttt
     1921 ggaatggtcc ttatgaacga ctggagtgca cgagacattc agaagtggga gtatgtccct
     1981 ctcgggccat tccttgggaa gagttttggg accactgtct ctccgtgggt ggtgcccatg
     2041 gatgctctca tgccctttgc tgtgcccaac ccgaagcagg accccaggcc cctgccgtat
     2101 ctgtgccatg acgagcccta cacatttgac atcaacctct ctgttaacct gaaaggagaa
     2161 ggaatgagcc aggcggctac catatgcaag tccaatttta agtacatgta ctggacgatg
     2221 ctgcagcagc tcactcacca ctctgtcaac ggctgcaacc tgcggccggg ggacctcctg
     2281 gcttctggga ccatcagcgg gccggagcca gaaaacttcg gctccatgtt ggaactgtcg
     2341 tggaagggaa cgaagcccat agacctgggg aatggtcaga ccaggaagtt tctgctggac
     2401 ggggatgaag tcatcataac agggtactgc cagggggatg gttaccgcat cggctttggc
     2461 cagtgtgctg gaaaagtgct gcctgctctc ctgccatcat gagattttct ctgctcttct
     2521 ggactcgacg atccaccgga tctagataac tgatcaattc caactgagcg ccggtcgcta
     2581 ccattaccaa cttgtctggt gtcaaaaata atagggatct acatctcgct ttcttgctgt
     2641 ccaatttcta ttaaaggttc ctttgttccc taagtccaac tactaaactg ggggatatta
     2701 tgaagggcct tgagcatctg gattctgcct aataaaaaac atttattttc attgcaatga
     2761 tgtatttaaa ttatttctga atattttact aaaaagggaa tgtgggaggt cagtgcattt
     2821 aaaacataaa gaaagtaggt aaccctacta gcaggtggca cttttcgggg aaatgtgcgc
     2881 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     2941 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     3001 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     3061 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     3121 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     3181 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     3241 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     3301 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     3361 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     3421 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     3481 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     3541 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     3601 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     3661 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     3721 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     3781 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     3841 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     3901 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     3961 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     4021 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     4081 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     4141 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     4201 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     4261 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     4321 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     4381 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     4441 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     4501 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     4561 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     4621 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgtacaagct tatgcatgtt
     4681 taaacatggt gcggacgtag ataagtagca tggcgggtta atcattaact acaaggaacc
     4741 cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg
     4801 accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg
     4861 cagcatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     4921 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     4981 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     5041 cagaaccgtc acgagcatca tcctctgcat ggtcaggtca tggatgagca gacgatggtg
     5101 caggatatcc tgctgatgaa gcagaacaac tttaacgccg tgcgctgttc gcattatccg
     5161 aaccatccgc tgtggtacac gctgtgcgac cgctacggcc tgtatgtggt ggatgaagcc
     5221 aatattgaaa cccacggcat ggtgccaatg aatcgtctga ccgatgatcc gcgctggcta
     5281 ccggcgatga gcgaacgcgt aacgcgaatg gtgcagcgcg atcgtaatca cccgagtgtg
     5341 atcatctggt cgctggggaa tgaatcaggc cacggcgcta atcacgacgc gctgtatcgc
     5401 tggatcaaat ctgtcgatcc ttcccgcccg gtgcagtatg aaggcggcgg agccgacacc
     5461 acggccaccg atattatttg cccgatgtac gcgcgcgtgg atgaagacca gcccttcccg
     5521 gctgtgccga aatggtccat caaaaaatgg ctttcgctac ctggagagac gcgcccgctg
     5581 atcctttgcg aatacgccca cgcgatgggt aacagtcttg gcggtttcgc taaatactgg
     5641 caggcgtttc gtcagtatcc ccgtttacag ggcggcttcg tctgggactg ggtggatcag
     5701 tcgctgatta aatatgatga aaacggcaac ccgtggtcgg cttacggcgg tgattttggc
     5761 gatacgccga acgatcgcca gttctgtatg aacggtctgg tctttgccga ccgcacgccg
     5821 catccagcgc tgacggaagc aaaacaccag cagcagtttt tccagttccg tttatccggg
     5881 caaaccatcg aagtgaccag cgaatacctg ttccgtcata gcgataacga gctcctgcac
     5941 tggatggtgg cgctggatgg taagccgctg gcaagcggtg aagtgcctct ggatgtcgct
     6001 ccacaaggta aacagttgat tgaactgcct gaactaccgc agccggagag cgccgggcaa
     6061 ctctggctca cagtacgcgt agtgcaaccg aacgcgaccg catggtcaga agccgggcac
     6121 atcagcgcct ggcagcagtg gcgtctggcg gaaaacctca gtgtgacgct ccccgccgcg
     6181 tcccacgcca tcccgcatct gaccaccagc gaaatggatt tttgcatcga gctgggtaat
     6241 aagcgttggc aatttaaccg ccagtcaggc tttctttcac agatgtggat tggcgataaa
     6301 aaacaactgc tgacgccgct gcgcgatcag ttcacccgtg caccgctgga taacgacatt
     6361 ggcgtaagtg aagcgacccg cattgaccct aacgcctggg tcgaacgctg gaaggcggcg
     6421 ggccattacc aggccgaagc agcgttgttg cagtgcacgg cagatacact tgctgatgcg
     6481 gtgctgatta cgaccgctca cgcgtggcag catcagggga aaaccttatt tatcagccgg
     6541 aaaacctacc ggattgatgg tagtggtcaa atggcgatta ccgttgatgt tgaagtggcg
     6601 agcgatacac cgcatccggc gcggattggc ctgaactgcc agctgcgcgc tcgctcgctc
     6661 actgaggccg cccgggcaaa gcccgggcgt cgggcgacct ttggtcgccc ggcctcagtg
     6721 agcgagcgag cgcgcagaga gggagtggcc aactccatca ctaggggttc ct
//
