LOCUS       62056_2375        7010 bp DNA     circular SYN 27-JUN-2020
DEFINITION  Cloning vector pAAV-EF1A-PSD95.FingR-GFP-CCR5TC, complete sequence.
ACCESSION   MT612429
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7010)
  AUTHORS   Bensussen S, Shankar S, Ching KH, Zemel D, Ta TL, Mount RA, Shroff 
            SN, Gritton HJ, Fabris P, Vanbenschoten H, Beck C, Man H-Y., Han X.
  TITLE     A viral toolbox of genetically encoded fluorescent synaptic tags
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7010)
  AUTHORS   Bensussen S, Shankar S, Ching KH, Zemel D, Ta TL, Mount RA, Shroff 
            SN, Gritton HJ, Fabris P, Vanbenschoten H, Beck C, Man H-Y., Han X.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2020) Biomedical Engineering, Boston University, 
            44 Cummington Mall, Boston, MA 02215, USA
REFERENCE   3  (bases 1 to 7010)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-JUN-2020) Biomedical Engineering, Boston University, 44 
            Cummington Mall, Boston, MA 02215, USA"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7010
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    228..249
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        264..294
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    302..318
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     326..342
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    379..569
                     /label=5' ITR
                     /note="5' ITR"
     misc_feature    621..632
                     /label=CCR5 binding site
                     /note="CCR5 binding site"
     promoter        644..1822
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
     promoter        1839..1857
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2181..2204
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             2229..2939
                     /label=EGFP
                     /note="enhanced GFP"
     misc_feature    2985..3506
                     /label=CCR5TC
                     /note="CCR5TC"
     misc_feature    3519..4107
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     primer_bind     complement(4110..4126)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    complement(4156..4277)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    4380..4569
                     /label=3' ITR
                     /note="3' ITR"
     primer_bind     complement(4587..4603)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      4745..5200
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5226..5330
                     /label=AmpR promoter
     CDS             5331..6188
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      6362..6950
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
       61 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      121 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
      181 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
      241 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
      301 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccagattt
      361 aattaaggcc ttaattaggc tgcgcgctcg ctcgctcact gaggccgccc gggcaaagcc
      421 cgggcgtcgg gcgacctttg gtcgcccggc ctcagtgagc gagcgagcgc gcagagaggg
      481 agtggccaac tccatcacta ggggttcctt gtagttaatg attaacccgc catgctactt
      541 atctacgtag ccatgctcta ggaagatctg gacagcagag atccagtttg gatcgataag
      601 cttgatatcg aattacgcgt gtcatcctca tcgaattccg tgaggctccg gtgcccgtca
      661 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg
      721 aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct
      781 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt
      841 tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg
      901 gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca cctggctgca
      961 gtacgtgatt cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg
     1021 cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg
     1081 ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc
     1141 catttaaaat ttttgatgac ctgctgcgac gctttttttc tggcaagata gtcttgtaaa
     1201 tgcgggccaa gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc
     1261 ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat
     1321 cggacggggg tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg
     1381 tatcgccccg ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag
     1441 atggccgctt cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga
     1501 gcgggcgggt gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc
     1561 atgtgactcc acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg
     1621 gagtacgtcg tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga
     1681 gtgggtggag actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc
     1741 cctttttgag tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt
     1801 tcttccattt caggtgtcgt gaggaattag cttggtacta atacgactca ctatagggag
     1861 acccaagctg gctaggtaag cttggtaccg agctcggatc cgccgccacc atgctcgaag
     1921 tcaaggaagc atcaccaacc agcatccaga tcagctgggt gctccacttg cgccacgttc
     1981 gctactaccg catcacctac ggtgaaactg gtggcaatag ccctgtccag gaattcaccg
     2041 tgcctggcag caagtccact gctaccatca gcggcctgaa acctggtgtc gactatacca
     2101 tcacggtgta cgccgtcacg atcttcagcg cctaccgctc cgcctggccg ccgatctcca
     2161 tcaactaccg caccggaacc gattacaagg atgacgacga taagggtagc ggctccagta
     2221 gatctggggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
     2281 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
     2341 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
     2401 ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca
     2461 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
     2521 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
     2581 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
     2641 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga
     2701 agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc
     2761 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
     2821 accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca
     2881 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtact
     2941 ctaggggagc tggcgctgga gcgggtgcag gggctggctc tagattccag tgccggatct
     3001 gcatgcggaa cttcagcgac cggtccaacc tgagcaggca catcagaacc cacaccggag
     3061 aaaagccctt cgcctgcgac atttgcggcc ggaagttcgc catcagcagc aacctgaaca
     3121 gccacaccaa gatccacact ggcagccaga aacctttcca gtgcagaatt tgtatgagaa
     3181 actttagcag aagcgacaac ctggccagac acatccggac acatactggt gaaaaacctt
     3241 ttgcctgtga tatctgtggc agaaagtttg ccacctccgg caatctgacc cggcacacaa
     3301 agattcacct gcggggcagc cagctatcga ttgtcgacgc tcctgaacaa cgtgaaggtg
     3361 cttctcaagt ttctgtttct gttacttttg aagatgttgc tgttcttttt actcgtgatg
     3421 aatggaaaaa acttgatctt tctcaacgtt ctctttatcg tgaagttatg cttgaaaatt
     3481 attctaatct tgcttctatg gcttaaaagc ttatcgataa tcaacctctg gattacaaaa
     3541 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     3601 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     3661 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     3721 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     3781 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     3841 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     3901 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc
     3961 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     4021 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     4081 ggatctccct ttgggccgcc tccccgcatc gataccgtcg acctcgaccc gggcggccgc
     4141 ttcgagcaga catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt
     4201 gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa
     4261 gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg
     4321 agatgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtaaa atcgataagg
     4381 atcttcctag agcatggcta cgtagataag tagcatggcg ggttaatcat taactacaag
     4441 gaacccctag tgatggagtt ggccactccc tctctgcgcg ctcgctcgct cactgaggcc
     4501 gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg cggcctcagt gagcgagcga
     4561 gcgcgcagcc ttaattaacc taattcactg gccgtcgttt tacaacgtcg tgactgggaa
     4621 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     4681 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     4741 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     4801 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     4861 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     4921 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     4981 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     5041 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     5101 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     5161 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa
     5221 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     5281 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     5341 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     5401 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     5461 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     5521 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     5581 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     5641 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     5701 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     5761 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     5821 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     5881 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     5941 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     6001 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     6061 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     6121 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     6181 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     6241 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     6301 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     6361 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     6421 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     6481 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     6541 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     6601 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     6661 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     6721 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     6781 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     6841 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     6901 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     6961 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
//
