LOCUS       pAAV-DNMT3A(human)      7390 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V019007
VERSION     V019007
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     repeat_region   1..141
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     enhancer        221..524
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        525..727
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     intron          876..1254
                     /note="fusion between human cytomegalovirus intron A and
                     human β-globin intron 2"
                     /label="chimeric intron"
     CDS             1338..4076
                     /codon_start=1
                     /product="DNA methyltransferase 3A"
                     /transl_table=1
                     /translation="MPAMPSSGPGDTSSSAAEREEDRKDGEEQEEPRGKEERQEPSTTA
                     RKVGRPGRKRKHPPVESGDTPKDPAVISKSPSMAQDSGASELLPNGDLEKRSEPQPEEG
                     SPAGGQKGGAPAEGEGAAETLPEASRAVENGCCTPKEGRGAPAEAGKEQKETNIESMKM
                     EGSRGRLRGGLGWESSLRQRPMPRLTFQAGDPYYISKRKRDEWLARWKREAEKKAKVIA
                     GMNAVEENQGPGESQKVEEASPPAVQQPTDPASPTVATTPEPVGSDAGDKNATKAGDDE
                     PEYEDGRGFGIGELVWGKLRGFSWWPGRIVSWWMTGRSRAAEGTRWVMWFGDGKFSVVC
                     VEKLMPLSSFCSAFHQATYNKQPMYRKAIYEVLQVASSRAGKLFPVCHDSDESDTAKAV
                     EVQNKPMIEWALGGFQPSGPKGLEPPEEEKNPYKEVYTDMWVEPEAAAYAPPPPAKKPR
                     KSTAEKPKVKEIIDERTRERLVYEVRQKCRNIEDICISCGSLNVTLEHPLFVGGMCQNC
                     KNCFLECAYQYDDDGYQSYCTICCGGREVLMCGNNNCCRCFCVECVDLLVGPGAAQAAI
                     KEDPWNCYMCGHKGTYGLLRRREDWPSRLQMFFANNHDQEFDPPKVYPPVPAEKRKPIR
                     VLSLFDGIATGLLVLKDLGIQVDRYIASEVCEDSITVGMVRHQGKIMYVGDVRSVTQKH
                     IQEWGPFDLVIGGSPCNDLSIVNPARKGLYEGTGRLFFEFYRLLHDARPKEGDDRPFFW
                     LFENVVAMGVSDKRDISRFLESNPVMIDAKEVSAAHRARYFWGNLPGMNRPLASTVNDK
                     LELQECLEHGRIAKFSKVRTITTRSNSIKQGKDQHFPVFMNEKEDILWCTEMERVFGFP
                     VHYTDVSNMSRLARQRLLGRSWSVPVIRHLFAPLKEYFACV*"
                     /label="DNMT3A"
     polyA_signal    4137..4613
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     repeat_region   4653..4793
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     rep_origin      4868..5323
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        5605..5709
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             5710..6570
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      6741..7329
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtggagc tagttattaa tagtaatcaa
      181 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
      241 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
      301 ttcccatagt aacgtcaata gggactttcc attgacgtca atgggtggag tatttacggt
      361 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
      421 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
      481 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
      541 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
      601 ttgacgtcaa tgggagtttg ttttgcacca aaatcaacgg gactttccaa aatgtcgtaa
      661 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
      721 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
      781 ccatagaaga caccgggacc gatccagcct ccgcggattc gaatcccggc cgggaacggt
      841 gcattggaac gcggattccc cgtgccaaga gtgacgtaag taccgcctat agagtctata
      901 ggcccacaaa aaatgctttc ttcttttaat atactttttt gtttatctta tttctaatac
      961 tttccctaat ctctttcttt cagggcaata atgatacaat gtatcatgcc tctttgcacc
     1021 attctaaaga ataacagtga taatttctgg gttaaggcaa tagcaatatt tctgcatata
     1081 aatatttctg catataaatt gtaactgatg taagaggttt catattgcta atagcagcta
     1141 caatccagct accattctgc ttttatttta tggttgggat aaggctggat tattctgagt
     1201 ccaagctagg cccttttgct aatcatgttc atacctctta tcttcctccc acagctcctg
     1261 ggcaacgtgc tggtctgtgt gctggcccat cactttggca aagaattggg attcgaacat
     1321 cgattgaatt cgccaccatg cccgccatgc cctccagcgg ccccggggac accagcagct
     1381 ctgctgcgga gcgggaggag gaccgaaagg acggagagga gcaggaggag ccgcgtggca
     1441 aggaggagcg ccaagagccc agcaccacgg cacggaaggt ggggcggcct gggaggaagc
     1501 gcaagcaccc cccggtggaa agcggtgaca cgccaaagga ccctgcggtg atctccaagt
     1561 ccccatccat ggcccaggac tcaggcgcct cagagctatt acccaatggg gacttggaga
     1621 agcggagtga gccccagcca gaggagggga gccctgctgg ggggcagaag ggcggggccc
     1681 cagcagaggg agagggtgca gctgagaccc tgcctgaagc ctcaagagca gtggaaaatg
     1741 gctgctgcac ccccaaggag ggccgaggag cccctgcaga agcgggcaaa gaacagaagg
     1801 agaccaacat cgaatccatg aaaatggagg gctcccgggg ccggctgcgg ggtggcttgg
     1861 gctgggagtc cagcctccgt cagcggccca tgccgaggct caccttccag gcgggggacc
     1921 cctactacat cagcaagcgc aagcgggacg agtggctggc acgctggaaa agggaggctg
     1981 agaagaaagc caaggtcatt gcaggaatga atgctgtgga agaaaaccag gggcccgggg
     2041 agtctcagaa ggtggaggag gccagccctc ctgctgtgca gcagcccact gaccccgcat
     2101 cccccactgt ggctaccacg cctgagcccg tggggtccga tgctggggac aagaatgcca
     2161 ccaaagcagg cgatgacgag ccagagtacg aggacggccg gggctttggc attggggagc
     2221 tggtgtgggg gaaactgcgg ggcttctcct ggtggccagg ccgcattgtg tcttggtgga
     2281 tgacgggccg gagccgagca gctgaaggca cccgctgggt catgtggttc ggagacggca
     2341 aattctcagt ggtgtgtgtt gagaagctga tgccgctgag ctcgttttgc agtgcgttcc
     2401 accaggccac gtacaacaag cagcccatgt accgcaaagc catctacgag gtcctgcagg
     2461 tggccagcag ccgcgcgggg aagctgttcc cggtgtgcca cgacagcgat gagagtgaca
     2521 ctgccaaggc cgtggaggtg cagaacaagc ccatgattga atgggccctg gggggcttcc
     2581 agccttctgg ccctaagggc ctggagccac cagaagaaga gaagaatccc tacaaagaag
     2641 tgtacacgga catgtgggtg gaacctgagg cagctgccta cgcaccacct ccaccagcca
     2701 aaaagccccg gaagagcaca gcggagaagc ccaaggtcaa ggagattatt gatgagcgca
     2761 caagagagcg gctggtgtac gaggtgcggc agaagtgccg gaacattgag gacatctgca
     2821 tctcctgtgg gagcctcaat gttaccctgg aacaccccct cttcgttgga ggaatgtgcc
     2881 aaaactgcaa gaactgcttt ctggagtgtg cgtaccagta cgacgacgac ggctaccagt
     2941 cctactgcac catctgctgt gggggccgtg aggtgctcat gtgcggaaac aacaactgct
     3001 gcaggtgctt ttgcgtggag tgtgtggacc tcttggtggg gccgggggct gcccaggcag
     3061 ccattaagga agacccctgg aactgctaca tgtgcgggca caagggtacc tacgggctgc
     3121 tgcggcggcg agaggactgg ccctcccggc tccagatgtt cttcgctaat aaccacgacc
     3181 aggaatttga ccctccaaag gtttacccac ctgtcccagc tgagaagagg aagcccatcc
     3241 gggtgctgtc tctctttgat ggaatcgcta cagggctcct ggtgctgaag gacttgggca
     3301 ttcaggtgga ccgctacatt gcctcggagg tgtgtgagga ctccatcacg gtgggcatgg
     3361 tgcggcacca ggggaagatc atgtacgtcg gggacgtccg cagcgtcaca cagaagcata
     3421 tccaggagtg gggcccattc gatctggtga ttgggggcag tccctgcaat gacctctcca
     3481 tcgtcaaccc tgctcgcaag ggcctctacg agggcactgg ccggctcttc tttgagttct
     3541 accgcctcct gcatgatgcg cggcccaagg agggagatga tcgccccttc ttctggctct
     3601 ttgagaatgt ggtggccatg ggcgttagtg acaagaggga catctcgcga tttctcgagt
     3661 ccaaccctgt gatgattgat gccaaagaag tgtcagctgc acacagggcc cgctacttct
     3721 ggggtaacct tcccggtatg aacaggccgt tggcatccac tgtgaatgat aagctggagc
     3781 tgcaggagtg tctggagcat ggcaggatag ccaagttcag caaagtgagg accattacta
     3841 cgaggtcaaa ctccataaag cagggcaaag accagcattt tcctgtcttc atgaatgaga
     3901 aagaggacat cttatggtgc actgaaatgg aaagggtatt tggtttccca gtccactata
     3961 ctgacgtctc caacatgagc cgcttggcga ggcagagact gctgggccgg tcatggagcg
     4021 tgccagtcat ccgccacctc ttcgctccgc tgaaggagta ttttgcgtgt gtgtaaggat
     4081 cctctagagt cgacctgcag aagcttgcct cgagcagcgc tgctcgagag atctacgggt
     4141 ggcatccctg tgacccctcc ccagtgcctc tcctggccct ggaagttgcc actccagtgc
     4201 ccaccagcct tgtcctaata aaattaagtt gcatcatttt gtctgactag gtgtccttct
     4261 ataatattat ggggtggagg ggggtggtat ggagcaaggg gcaagttggg aagacaacct
     4321 gtagggcctg cggggtctat tgggaaccaa gctggagtgc agtggcacaa tcttggctca
     4381 ctgcaatctc cgcctcctgg gttcaagcga ttctcctgcc tcagcctccc gagttgttgg
     4441 gattccaggc atgcatgacc aggctcagct aatttttgtt tttttggtag agacggggtt
     4501 tcaccatatt ggccaggctg gtctccaact cctaatctca ggtgatctac ccaccttggc
     4561 ctcccaaatt gctgggatta caggcgtgaa ccactgctcc cttccctgtc cttctgattt
     4621 tgtaggtaac cacgtgcgga ccgagcggcc gcaggaaccc ctagtgatgg agttggccac
     4681 tccctctctg cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg cccgacgccc
     4741 gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc agctgcctgc aggggcgcct
     4801 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatac gtcaaagcaa
     4861 ccatagtacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     4921 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     4981 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     5041 cgatttagtg ctttacggca cctcgacccc aaaaaacttg atttgggtga tggttcacgt
     5101 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     5161 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggg ctattctttt
     5221 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     5281 aaatttaacg cgaattttaa caaaatatta acgtttacaa ttttatggtg cactctcagt
     5341 acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac
     5401 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
     5461 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc
     5521 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     5581 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     5641 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     5701 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     5761 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     5821 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     5881 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     5941 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     6001 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     6061 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     6121 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     6181 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     6241 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     6301 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     6361 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     6421 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     6481 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     6541 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     6601 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     6661 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     6721 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     6781 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     6841 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     6901 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     6961 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     7021 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     7081 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     7141 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     7201 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     7261 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     7321 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     7381 gctcacatgt
//
