LOCUS       V013850                 7333 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013850
VERSION     V013850
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7333)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7333
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             6..1868
                     /gene="Rep78"
                     /label="Protein Rep78"
                     /note="Protein Rep78 from Adeno-associated virus 2 (isolate
                     Srivastava/1982). Accession#: Q89268"
     CDS             3943..3954
                     /label="WELQut site"
                     /note="WELQut protease recognition and cleavage site"
     promoter        complement(4406..4424)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4445..4461)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(4469..4485)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4493..4523)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4538..4559)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4847..5435)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5609..6466)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6467..6571)
                     /label="AmpR promoter"
     rep_origin      complement(6597..7052)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     7194..7210
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        7220..7238
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     7268..7284
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
ORIGIN
        1 ccgccatgcc ggggttttac gagattgtga ttaaggtccc cagcgacctt gacgagcatc
       61 tgcccggcat ttctgacagc tttgtgaact gggtggccga gaaggaatgg gagttgccgc
      121 cagattctga catggatctg aatctgattg agcaggcacc cctgaccgtg gccgagaagc
      181 tgcagcgcga ctttctgacg gaatggcgcc gtgtgagtaa ggccccggag gctcttttct
      241 ttgtgcaatt tgagaaggga gagagctact tccacatgca cgtgctcgtg gaaaccaccg
      301 gggtgaaatc catggttttg ggacgtttcc tgagtcagat tcgcgaaaaa ctgattcaga
      361 gaatttaccg cgggatcgag ccgactttgc caaactggtt cgcggtcaca aagaccagaa
      421 atggcgccgg aggcgggaac aaggtggtgg atgagtgcta catccccaat tacttgctcc
      481 ccaaaaccca gcctgagctc cagtgggcgt ggactaatat ggaacagtat ttaagcgcct
      541 gtttgaatct cacggagcgt aaacggttgg tggcgcagca tctgacgcac gtgtcgcaga
      601 cgcaggagca gaacaaagag aatcagaatc ccaattctga tgcgccggtg atcagatcaa
      661 aaacttcagc caggtacatg gagctggtcg ggtggctcgt ggacaagggg attacctcgg
      721 agaagcagtg gatccaggag gaccaggcct catacatctc cttcaatgcg gcctccaact
      781 cgcggtccca aatcaaggct gccttggaca atgcgggaaa gattatgagc ctgactaaaa
      841 ccgcccccga ctacctggtg ggccagcagc ccgtggagga catttccagc aatcggattt
      901 ataaaatttt ggaactaaac gggtacgatc cccaatatgc ggcttccgtc tttctgggat
      961 gggccacgaa aaagttcggc aagaggaaca ccatctggct gtttgggcct gcaactaccg
     1021 ggaagaccaa catcgcggag gccatagccc acactgtgcc cttctacggg tgcgtaaact
     1081 ggaccaatga gaactttccc ttcaacgact gtgtcgacaa gatggtgatc tggtgggagg
     1141 aggggaagat gaccgccaag gtcgtggagt cggccaaagc cattctcgga ggaagcaagg
     1201 tgcgcgtgga ccagaaatgc aagtcctcgg cccagataga cccgactccc gtgatcgtca
     1261 cctccaacac caacatgtgc gccgtgattg acgggaactc aacgaccttc gaacaccagc
     1321 agccgttgca agaccggatg ttcaaatttg aactcacccg ccgtctggat catgactttg
     1381 ggaaggtcac caagcaggaa gtcaaagact ttttccggtg ggcaaaggat cacgtggttg
     1441 aggtggagca tgaattctac gtcaaaaagg gtggagccaa gaaaagaccc gcccccagtg
     1501 acgcagatat aagtgagccc aaacgggtgc gcgagtcagt tgcgcagcca tcgacgtcag
     1561 acgcggaagc ttcgatcaac tacgcagaca ggtaccaaaa caaatgttct cgtcacgtgg
     1621 gcatgaatct gatgctgttt ccctgcagac aatgcgagag aatgaatcag aattcaaata
     1681 tctgcttcac tcacggacag aaagactgtt tagagtgctt tcccgtgtca gaatctcaac
     1741 ccgtttctgt cgtcaaaaag gcgtatcaga aactgtgcta cattcatcat atcatgggaa
     1801 aggtgccaga cgcttgcact gcctgcgatc tggtcaatgt ggatttggat gactgcatct
     1861 ttgaacaata aatgatttaa atcaggtatg gctgccgatg gttatcttcc agattggctc
     1921 gaggacactc tctctgaagg aataagacag tggtggaagc tcaaacctgg cccaccacca
     1981 ccaaagcccg cagagcggca taaggacgac agcaggggtc ttgtgcttcc tgggtacaag
     2041 tacctcggac ccttcaacgg actcgacaag ggagagccgg tcaacgaggc agacgccgcg
     2101 gccctcgagc acgacaaagc ctacgaccgg cagctcgaca gcggagacaa cccgtacctc
     2161 aagtacaacc acgccgacgc cgagttccag gagcggctca aagaagatac gtcttttggg
     2221 ggcaacctcg ggcgagcagt cttccaggcc aaaaagaggc ttcttgaacc tcttggtctg
     2281 gttgaggaag cggctaagac ggctcctgga aagaagaggc ctgtagagca ctctcctgtg
     2341 gagccagact cctcctcggg aaccggaaag gcgggccagc agcctgcaag aaaaagattg
     2401 aattttggtc agactggaga cgcagactca gtcccagacc ctcaaccaat cggagaacct
     2461 cccgcagccc cctcaggtgt gggatctctt acaatggctg caggcggtgg cgcaccaatg
     2521 gcagacaata acgagggcgc cgacggagtg ggtaattcct cgggaaattg gcattgcgat
     2581 tccacatgga tgggcgacag agtcatcacc accagcaccc gaacctgggc cctgcccacc
     2641 tacaacaacc acctctacaa gcaaatctcc aacagcacat ctggaggatc ttcaaatgac
     2701 aacgcctact tcggctacag caccccctgg gggtattttg actttaacag attccactgc
     2761 cacttttcac cacgtgactg gcagcgactc atcaacaaca actggggatt ccggcccaag
     2821 agactcagct tcaagctctt caacatccag gtcaaggagg tcacgcagaa tgaaggcacc
     2881 aagaccatcg ccaataacct caccagcacc atccaggtgt ttacggactc ggagtaccag
     2941 ctgccgtacg ttctcggctc tgcccaccag ggctgcctgc ctccgttccc ggcggacgtg
     3001 ttcatgattc cccagtacgg ctacctaaca ctcaacaacg gtagtcaggc cgtgggacgc
     3061 tcctccttct actgcctgga atactttcct tcgcagatgc tgagaaccgg caacaacttc
     3121 cagtttactt acaccttcga ggacgtgcct ttccacagca gctacgccca cagccagagc
     3181 ttggaccggc tgatgaatcc tctgattgac cagtacctgt actacttgtc tcggactcaa
     3241 acaacaggag gcacgacaaa tacgcagact ctgggcttca gccaaggtgg gcctaataca
     3301 atggccaatc aggcaaagaa ctggctgcca ggaccctgtt accgccagca gcgagtatca
     3361 aagacatctg cggataacaa caacagtgaa tactcgtgga ctggagctac caagtaccac
     3421 ctcaatggca gagactctct ggtgaatccg ggcccggcca tggcaagcca caaggacgat
     3481 gaagaaaagt tttttcctca gagcggggtt ctcatctttg ggaagcaagg ctcagagaaa
     3541 acaaatgtgg acattgaaaa ggtcatgatt acagacgaag aggaaatcag gacaaccaat
     3601 cccgtggcta cggagcagta tggttctgta tctaccaacc tccagagagg caacagacaa
     3661 gcagctaccg cagatgtcaa cacacaaggc gttcttccag gcatggtctg gcaggacaga
     3721 gatgtgtacc ttcaggggcc catctgggca aagattccac acacggacgg acattttcac
     3781 ccctctcccc tcatgggtgg attcggactt aaacaccctc cgcctcagat cctgatcaag
     3841 aacacgcctg tacctgcgga tcctccgacc accttcaacc agtcaaagct gaactctttc
     3901 atcacccagt attctactgg ccaagtcagc gtggagatcg agtgggagct gcagaaggaa
     3961 aacagcaagc gctggaaccc cgagatccag tacacctcca actactacaa atctacaagt
     4021 gtggactttg ctgttaatac agaaggcgtg tactctgaac cccgccccat tggcacccgt
     4081 tacctcaccc gtaatctgta attgcctgtt aatcaataaa ccggttgatt cgtttcagtt
     4141 gaactttggt ctctgcgaag ggcgaattcg tttaaacctg caggactaga ggtcctgtat
     4201 tagaggtcac gtgagtgttt tgcgacattt tgcgacacca tgtggtcacg ctgggtattt
     4261 aagcccgagt gagcacgcag ggtctccatt ttgaagcggg aggtttgaac gcgcagccgc
     4321 caagccgaat tctgcagata tccatcacac tggcggccgc tcgactagag cggccgccac
     4381 cgcggtggag ctccagcttt tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat
     4441 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     4501 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     4561 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     4621 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     4681 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     4741 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     4801 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     4861 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     4921 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     4981 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     5041 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     5101 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     5161 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     5221 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     5281 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     5341 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     5401 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     5461 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     5521 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     5581 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     5641 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     5701 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     5761 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     5821 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     5881 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     5941 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     6001 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     6061 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     6121 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     6181 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     6241 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     6301 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     6361 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     6421 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     6481 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     6541 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctaaat
     6601 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
     6661 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
     6721 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
     6781 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
     6841 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
     6901 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
     6961 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
     7021 cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg ccattcaggc tgcgcaactg
     7081 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
     7141 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac
     7201 gacggccagt gagcgcgcgt aatacgactc actatagggc gaattgggta ccgggccccc
     7261 cctcgatcga ggtcgacggt atcgggggag ctcgcagggt ctccattttg aagcgggagg
     7321 tttgaacgcg cag
//
