LOCUS       pAAV-Cd109(mouse)       8973 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V044493
VERSION     V044493
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     repeat_region   1..141
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     enhancer        221..524
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        525..727
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     intron          876..1254
                     /note="fusion between human cytomegalovirus intron A and
                     human β-globin intron 2"
                     /label="chimeric intron"
     CDS             1355..5680
                     /label="Cd109(NM_153098)"
                     /note="Cd109(NM_153098)"
                     /gene="Cd109"
     polyA_signal    5720..6196
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     repeat_region   6236..6376
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     rep_origin      6451..6906
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        7188..7292
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             7293..8153
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      8324..8912
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtggagc tagttattaa tagtaatcaa
      181 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
      241 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
      301 ttcccatagt aacgtcaata gggactttcc attgacgtca atgggtggag tatttacggt
      361 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
      421 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
      481 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
      541 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
      601 ttgacgtcaa tgggagtttg ttttgcacca aaatcaacgg gactttccaa aatgtcgtaa
      661 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
      721 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
      781 ccatagaaga caccgggacc gatccagcct ccgcggattc gaatcccggc cgggaacggt
      841 gcattggaac gcggattccc cgtgccaaga gtgacgtaag taccgcctat agagtctata
      901 ggcccacaaa aaatgctttc ttcttttaat atactttttt gtttatctta tttctaatac
      961 tttccctaat ctctttcttt cagggcaata atgatacaat gtatcatgcc tctttgcacc
     1021 attctaaaga ataacagtga taatttctgg gttaaggcaa tagcaatatt tctgcatata
     1081 aatatttctg catataaatt gtaactgatg taagaggttt catattgcta atagcagcta
     1141 caatccagct accattctgc ttttatttta tggttgggat aaggctggat tattctgagt
     1201 ccaagctagg cccttttgct aatcatgttc atacctctta tcttcctccc acagctcctg
     1261 ggcaacgtgc tggtctgtgt gctggcccat cactttggca aagaattggg attcgaacat
     1321 cgattgaatt ccccggggat cctctagagt cgacatgcgc agtcggaggc tcctgagcgc
     1381 ggcccacctt ctctgcttgt gcgcggtcgc gctggccgcc ccagggtccc gctttctggt
     1441 gacagcccca gggatcatca gacccggagc aaatgtgact attggggtgg atctcctgga
     1501 aaatagcccc ccacaggtcc ttgtaaaggc tcaggtgttc aagatagctt ccaacaaatc
     1561 gagatccatc ctggaagcag aaggagtctt tcacagaggc catttcaaga ctctcgttct
     1621 tccggcacta cctctgagca gtgcagataa gatttatgag ctacacataa acggacaatc
     1681 agagaatgag atcgtattct ccaacaggac acgtttaaca tttgagagca agagtatatc
     1741 tgtcctcatt cagacagaca aggcattcta caagccgaag caggaagtga agtttcgggt
     1801 gctcacactc tgctcagatc tcaagcctta taggacatcc gtggacattt tcattaagga
     1861 tccgaagtct aatgtgatcc aacagtggtt ttctcagaaa ggtgatcttg gagtcgtttc
     1921 caaaactttt caactatcct ctaatccaat atttggtgac tggtccattc aggttcaagt
     1981 gaatgatcag caatattacc agtcatttca ggtcctagaa tatgtgttac caaagttcga
     2041 agtcaccgtg cagaccccgc tgtattgttc cttgaagtct aaacagttaa atggctccgt
     2101 catagcaaag tatacatacg ggaagccagt gaagggaagt ctgtcactta catttttacc
     2161 tttgtctttt tggggaaaga agaagaatat cacaaaaagt tttgagataa atggatttgc
     2221 aaacttctct tttgataatt atgagatgaa aaaggtaatg aatttgaaac cgctaacgga
     2281 tgtctcggaa gggagttatg agaatgtgga cccgtccttt cctgggccgg cagagattat
     2341 agctactgtg acggaatcac tcacaggtat ctcaagaatg gcaagcacca atgtgttttt
     2401 caagcaacat gattacatca ttgagatttt tgattatacg actgtcttga aaccatctct
     2461 caacttcaca gccactgtga aggtcagccg ctctgatggc aatcaactaa ctcctgaaga
     2521 aatagaaaat gatttggtca cagtagtgac ccagcggaaa aataaccacc ctgagagtca
     2581 gagagatcag gaaatggatt atatccagac cgtaaattat actatccccc aaaatggaat
     2641 cattaagatt gaattcccag tcatgtccat ttccggcgag ctacaactga aggcttattt
     2701 tcttgatggt acaagttctg tgacagtcca cagtatgttc acctctccta gtaagacgta
     2761 catccagctt aaaaccagag atgaatatat aaaggtgggg tcaccttttg atttgatggt
     2821 tagtggcaac agacaattca aggacttaag ctatatggta atatcaaagg gacagttggt
     2881 ggctgcaggc aaacaaagtt caagaacttt ctctttaaca ccagaggctt cctgggctcc
     2941 aaaggcctgt ataatcgcat attatattgc agaggatggg gaaattataa atgatattct
     3001 aaaaatcccc gttcagcttg tttttgaaaa taaggtaaag ctgttttgga gtaaacctac
     3061 agtcaagcca tctgataagg tctctctcag gatctctgcg acacagtctg actccctggt
     3121 ggggattgta gctgttgaca aaagtgtaac attgatggaa aactccaaca gtattacaat
     3181 ggaaaccatg gtccatgaac tggaacttta taacacagaa tattatctag gcatgttcat
     3241 gaactccttc gctgtatttc aggaatgtgg tctctgggtg ttgacagatg caacccttat
     3301 aagagatagc attgatgaag tctacgatac tgaagagtat tctgaacgct ttgcagagga
     3361 aaatgaggca aacctggttg attttgaaga tgcttcttca gttaacaatg tacatgtcag
     3421 gaagaatttt ccagaaacat ggatttggct agacgcctac atgggatcta agatttatga
     3481 agaatttgaa gttactgtac ctgactccat cacttcttgg gtggcttcgg cttttgtcat
     3541 ctcagaagac ctgggttttg ggttaacaac cgttccagca gagctgcaag ccttccaacc
     3601 gtttttcctt ttcctgaatc ttccgtactc tgttatcaga ggtgaagagt ttgctttgga
     3661 agtatccatc gtcaattatt tgaaagatac aattaaggtt gtgatactca ttgaggaaag
     3721 tgacagcttt gatattttaa tgacttcaaa tgacaccaat ggcaccatat accggaagac
     3781 tgttcaagtt cccagggata atggggtcac tcttgttttt cccatcaagc ccacgcactt
     3841 gggagagatt cccatcacag tgacggctgc ctcacccact gcctctgatg ctgtcaccca
     3901 gacaattgta gtaaagcctg aagggataga aaagtcgtat tcgaaatctg tcttattgga
     3961 tctgacagat agcaacgtag aaagtaagca gcaatctatg agattctcct ttcctcctga
     4021 tacggtcatt ggcagtgaaa gagttcagat cacagcaatt ggagatatcc ttggttcctc
     4081 catcaatggc ttatcttcac tgatccggat gccttatgga tgtggtgaac agaacatgat
     4141 atattttgct ccaaatattt acattttgga ttatctgact aaacagaaac agctgacagt
     4201 taatttaaaa gaaaaggccc tttcatatat gaggcaaggt taccaaaggg agcttctcta
     4261 tcagagggaa gatggctcct tcagtgcttt tggggacatt gactcttctg ggagcacttg
     4321 gctgtcagca tttgttttaa gatgctttct ggaagctgat tactatatag atattgatca
     4381 ggatgtgtta cacagaacat atacttggct taatgcacat aagaaattca atggtgaatt
     4441 ttgggagcca ggaagagtga ttcacagtga acttcaaggt ggcaccaaaa gcccagtaac
     4501 acttacggcc tatattgtga cttctgtcct gggatacaaa aagtatcagc ctaatatcga
     4561 tgtacaagac tcaatcaagt ttttggaatt tgaattcagc agaggaattt cggacaatta
     4621 taccttagca attatatcct atgccctgtc cacagtcggg agccctaaag cagaggaggc
     4681 tttgaacttg ctgatgcagc gatcagaaaa ggaaggagac acacagttct ggttgtcatc
     4741 aggacctgcg ctctctgggt cctggcagcc gcgctccgtg gacatcgaaa tcgctgctta
     4801 tgcactgctg gcgcacactc tgcaccatgt gtctgaggga atcccggtta tgaggtggct
     4861 catccagcaa agaaacagcc tgggaggttt tgtatccact caggatactg ttgtggccct
     4921 gaaggcatta tctgaatttt cagcccttgt gcacaaggaa aatacagata tccaactgac
     4981 cgtcacaggg cccggcatcc ccagatccat acacttccga attgactccc agaacctctt
     5041 tctccttcac caggaagagc ttcatgcgct agatcccatc accgttaatg tttctgcgca
     5101 tggctcggga tttgctattt gccagcttaa tgttgactat aacgtgaaag gttcaggttc
     5161 ttctaaaaga cggagatcta ccgaaaatca agaagttttt gatttagacg tcattgtgaa
     5221 taatgaggac gacattagtc acctgaatct gaatgtgtgc acaagtcact tgggttcaga
     5281 gaggacaggc atggtcctta tggaagtgaa ccttctcagt ggctttagtg catcttcaga
     5341 ttcaattcct ctgagtgaga ccctgaagaa agtggaatat gataacggga aactcaacct
     5401 ttatttagat tctgtaaatg aatcccagtt ttgtgttaat attcccactg tgagagacta
     5461 caaagtttca aatattcgag atggttcagt atctgtaatg gactactatg aaccaaggag
     5521 acaggcagtg cgaagctaca acacccaggt gaagctgtcc tcatgttacc tcagtccaga
     5581 caccaactgc aagtcacaca cggacggagc cacggactcc cttcgacgtt cttcgagcct
     5641 tctcgtcttt tgctccgtcc ttctgtactt tgtgcaacat tgaaagcttg cctcgagcag
     5701 cgctgctcga gagatctacg ggtggcatcc ctgtgacccc tccccagtgc ctctcctggc
     5761 cctggaagtt gccactccag tgcccaccag ccttgtccta ataaaattaa gttgcatcat
     5821 tttgtctgac taggtgtcct tctataatat tatggggtgg aggggggtgg tatggagcaa
     5881 ggggcaagtt gggaagacaa cctgtagggc ctgcggggtc tattgggaac caagctggag
     5941 tgcagtggca caatcttggc tcactgcaat ctccgcctcc tgggttcaag cgattctcct
     6001 gcctcagcct cccgagttgt tgggattcca ggcatgcatg accaggctca gctaattttt
     6061 gtttttttgg tagagacggg gtttcaccat attggccagg ctggtctcca actcctaatc
     6121 tcaggtgatc tacccacctt ggcctcccaa attgctggga ttacaggcgt gaaccactgc
     6181 tcccttccct gtccttctga ttttgtaggt aaccacgtgc ggaccgagcg gccgcaggaa
     6241 cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg
     6301 cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg
     6361 cgcagctgcc tgcaggggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     6421 tcacaccgca tacgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg
     6481 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     6541 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     6601 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     6661 ttgatttggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     6721 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     6781 accctatctc gggctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     6841 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta
     6901 caattttatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc
     6961 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     7021 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     7081 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     7141 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     7201 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     7261 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     7321 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     7381 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     7441 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     7501 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     7561 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     7621 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     7681 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     7741 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     7801 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     7861 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     7921 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     7981 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     8041 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     8101 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     8161 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     8221 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     8281 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     8341 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     8401 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     8461 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     8521 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     8581 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     8641 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     8701 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     8761 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     8821 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     8881 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     8941 tcctggcctt ttgctggcct tttgctcaca tgt
//
