LOCUS       40924_3082        4452 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pAAV-CW3SSA-EGFP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4452)
  AUTHORS   Choi JH, Yu NK, Baek GC, Bakes J, Seo D, Nam HJ, Baek SH, Lim CS, 
            Lee YS, Kaang BK.
  TITLE     Optimization of AAV expression cassettes to improve packaging 
            capacity and transgene expression in neurons
  JOURNAL   Mol Brain 7 (1), 17 (2014)
  PUBMED    24618276
REFERENCE   2  (bases 1 to 4452)
  AUTHORS   Choi J-H., Kaang B-K.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2014) Department of Biological Sciences, Seoul 
            National University, Gwanak-gu, Seoul, Seoul 151-747, South Korea
REFERENCE   3  (bases 1 to 4452)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4452)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Brain";
            date: "2014"; volume: "7"; issue: "1"; pages: "17"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (07-FEB-2014) Department of Biological Sciences, Seoul National 
            University, Gwanak-gu, Seoul, Seoul 151-747, South Korea"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4452
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     CDS             567..1283
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    1301..1548
                     /label=WPRE3
                     /note="Truncated short version of woodchuck hepatitis virus
                     posttranscriptional regulatory element (248 bp)"
     polyA_signal    1653..1701
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     repeat_region   1715..1855
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      1930..2385
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2667..2771
                     /label=AmpR promoter
     CDS             2772..3629
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3803..4391
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca gatgctgcac acttgtggac taagtttgtt
      181 cgcatcccct tctccaaccc cctcagtaca tcaccctggg ggaacagggt ccacttgctc
      241 ctgggcccac acagtcctgc agtattgtgt atataaggcc agggcaaaga ggagcaggtt
      301 ttaaagtgaa aggcaggcag gtgttgggga ggcagttacc ggggcaacgg gaacagggcg
      361 tttcggaggt ggttgccatg gggacctgga tgctgacgaa ggctcgcgag gctgtgagca
      421 gccacagtgc cctgctcaga agccccaagc tcgtcagtca agccggttct ccgtttgcac
      481 tcaggagcac gggcaggcga gtggccccta gttctggggg cagcgtgaaa agacggtatc
      541 gataagcttg attcgagcta gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg
      601 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
      661 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
      721 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct
      781 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
      841 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
      901 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
      961 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
     1021 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca
     1081 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
     1141 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc
     1201 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
     1261 tcggcatgga cgagctgtac aagtaaatcg aattctcgag ataatcaacc tctggattac
     1321 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     1381 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     1441 tccttgtata aatcctggtt agttcttgcc acggcggaac tcatcgccgc ctgccttgcc
     1501 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt tatttgtgaa
     1561 atttgtgatg ctattgcttt atttgtaacc attctagctt tatttgtgaa atttgtgatg
     1621 ctattgcttt atttgtaacc attataagct gcaataaaag atctttattt tcattagatc
     1681 tgtgtgttgg ttttttgtgt gatcgagcgg ccgcaggaac ccctagtgat ggagttggcc
     1741 actccctctc tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc
     1801 ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc gcagctgcct gcaggggcgc
     1861 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat acgtcaaagc
     1921 aaccatagta cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     1981 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     2041 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     2101 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgatttgggt gatggttcac
     2161 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     2221 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg ggctattctt
     2281 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     2341 aaaaatttaa cgcgaatttt aacaaaatat taacgtttac aattttatgg tgcactctca
     2401 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
     2461 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     2521 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg
     2581 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     2641 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     2701 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     2761 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     2821 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     2881 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     2941 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     3001 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     3061 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     3121 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     3181 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     3241 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     3301 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     3361 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     3421 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     3481 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     3541 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     3601 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     3661 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     3721 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     3781 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     3841 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     3901 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     3961 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     4021 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     4081 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     4141 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     4201 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     4261 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     4321 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     4381 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     4441 ttgctcacat gt
//
