LOCUS       40924_3072        4406 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pAAV-CW3SA-EGFP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4406)
  AUTHORS   Choi JH, Yu NK, Baek GC, Bakes J, Seo D, Nam HJ, Baek SH, Lim CS, 
            Lee YS, Kaang BK.
  TITLE     Optimization of AAV expression cassettes to improve packaging 
            capacity and transgene expression in neurons
  JOURNAL   Mol Brain 7 (1), 17 (2014)
  PUBMED    24618276
REFERENCE   2  (bases 1 to 4406)
  AUTHORS   Choi J-H., Kaang B-K.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2014) Department of Biological Sciences, Seoul 
            National University, Gwanak-gu, Seoul, Seoul 151-747, South Korea
REFERENCE   3  (bases 1 to 4406)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4406)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Brain";
            date: "2014"; volume: "7"; issue: "1"; pages: "17"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (07-FEB-2014) Department of Biological Sciences, Seoul National 
            University, Gwanak-gu, Seoul, Seoul 151-747, South Korea"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4406
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     CDS             567..1283
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    1301..1547
                     /label=WPRE3
                     /note="Truncated short version of woodchuck hepatitis virus
                     posttranscriptional regulatory element (248 bp)"
     polyA_signal    1607..1655
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     repeat_region   1669..1809
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      1884..2339
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2621..2725
                     /label=AmpR promoter
     CDS             2726..3583
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3757..4345
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca gatgctgcac acttgtggac taagtttgtt
      181 cgcatcccct tctccaaccc cctcagtaca tcaccctggg ggaacagggt ccacttgctc
      241 ctgggcccac acagtcctgc agtattgtgt atataaggcc agggcaaaga ggagcaggtt
      301 ttaaagtgaa aggcaggcag gtgttgggga ggcagttacc ggggcaacgg gaacagggcg
      361 tttcggaggt ggttgccatg gggacctgga tgctgacgaa ggctcgcgag gctgtgagca
      421 gccacagtgc cctgctcaga agccccaagc tcgtcagtca agccggttct ccgtttgcac
      481 tcaggagcac gggcaggcga gtggccccta gttctggggg cagcgtgaaa agacggtatc
      541 gataagcttg attcgagcta gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg
      601 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
      661 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
      721 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct
      781 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
      841 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
      901 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
      961 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
     1021 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca
     1081 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
     1141 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc
     1201 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
     1261 tcggcatgga cgagctgtac aagtaaatcg aattctcgag ataatcaacc tctggattac
     1321 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     1381 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     1441 tccttgtata aatcctggtt agttcttgcc acggcggaac tcatcgccgc ctgccttgcc
     1501 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtcta gctttatttg
     1561 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aaagatcttt
     1621 attttcatta gatctgtgtg ttggtttttt gtgtgatcga gcggccgcag gaacccctag
     1681 tgatggagtt ggccactccc tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa
     1741 aggtcgcccg acgcccgggc tttgcccggg cggcctcagt gagcgagcga gcgcgcagct
     1801 gcctgcaggg gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     1861 gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     1921 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     1981 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     2041 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgattt
     2101 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     2161 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     2221 ctcgggctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     2281 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt
     2341 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc
     2401 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
     2461 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
     2521 cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
     2581 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     2641 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     2701 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     2761 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     2821 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     2881 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     2941 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     3001 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     3061 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     3121 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     3181 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     3241 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     3301 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     3361 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     3421 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     3481 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     3541 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     3601 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     3661 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     3721 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     3781 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     3841 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     3901 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     3961 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     4021 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     4081 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     4141 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     4201 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     4261 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     4321 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     4381 cttttgctgg ccttttgctc acatgt
//
