LOCUS       40924_3067        4428 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pAAV-CW3L-EGFP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4428)
  AUTHORS   Choi JH, Yu NK, Baek GC, Bakes J, Seo D, Nam HJ, Baek SH, Lim CS, 
            Lee YS, Kaang BK.
  TITLE     Optimization of AAV expression cassettes to improve packaging 
            capacity and transgene expression in neurons
  JOURNAL   Mol Brain 7 (1), 17 (2014)
  PUBMED    24618276
REFERENCE   2  (bases 1 to 4428)
  AUTHORS   Choi J-H., Kaang B-K.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2014) Department of Biological Sciences, Seoul 
            National University, Gwanak-gu, Seoul, Seoul 151-747, South Korea
REFERENCE   3  (bases 1 to 4428)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4428)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Brain";
            date: "2014"; volume: "7"; issue: "1"; pages: "17"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (07-FEB-2014) Department of Biological Sciences, Seoul National 
            University, Gwanak-gu, Seoul, Seoul 151-747, South Korea"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4428
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     CDS             567..1283
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    1301..1547
                     /label=WPRE3
                     /note="Truncated short version of woodchuck hepatitis virus
                     posttranscriptional regulatory element (248 bp)"
     repeat_region   1691..1831
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      1906..2361
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2643..2747
                     /label=AmpR promoter
     CDS             2748..3605
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3779..4367
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca gatgctgcac acttgtggac taagtttgtt
      181 cgcatcccct tctccaaccc cctcagtaca tcaccctggg ggaacagggt ccacttgctc
      241 ctgggcccac acagtcctgc agtattgtgt atataaggcc agggcaaaga ggagcaggtt
      301 ttaaagtgaa aggcaggcag gtgttgggga ggcagttacc ggggcaacgg gaacagggcg
      361 tttcggaggt ggttgccatg gggacctgga tgctgacgaa ggctcgcgag gctgtgagca
      421 gccacagtgc cctgctcaga agccccaagc tcgtcagtca agccggttct ccgtttgcac
      481 tcaggagcac gggcaggcga gtggccccta gttctggggg cagcgtgaaa agacggtatc
      541 gataagcttg attcgagcta gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg
      601 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
      661 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
      721 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct
      781 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
      841 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
      901 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
      961 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
     1021 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca
     1081 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
     1141 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc
     1201 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
     1261 tcggcatgga cgagctgtac aagtaaatcg aattctcgag ataatcaacc tctggattac
     1321 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     1381 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     1441 tccttgtata aatcctggtt agttcttgcc acggcggaac tcatcgccgc ctgccttgcc
     1501 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtcta gctttatttg
     1561 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
     1621 caacaacaat tgcattcatt ttatgtttca ggttcagggg gagatgtggg aggtttttta
     1681 aagcggccgc aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg
     1741 ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca
     1801 gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga tgcggtattt tctccttacg
     1861 catctgtgcg gtatttcaca ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg
     1921 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     1981 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     2041 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     2101 tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga
     2161 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     2221 ctggaacaac actcaaccct atctcgggct attcttttga tttataaggg attttgccga
     2281 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     2341 aaatattaac gtttacaatt ttatggtgca ctctcagtac aatctgctct gatgccgcat
     2401 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     2461 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     2521 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
     2581 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     2641 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     2701 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     2761 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     2821 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     2881 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     2941 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     3001 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     3061 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     3121 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     3181 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     3241 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     3301 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     3361 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     3421 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     3481 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     3541 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     3601 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     3661 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     3721 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     3781 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     3841 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     3901 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     3961 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     4021 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     4081 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     4141 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     4201 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     4261 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     4321 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     4381 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgt
//
