LOCUS       62056_2310        4840 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4840)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4840
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        154..533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        534..737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             763..1470
                     /codon_start=1
                     /label=mCherry
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     polyA_signal    1530..1737
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        1745..1985
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_RNA        2014..2089
                     /label=Sa gRNA scaffold
                     /note="guide RNA scaffold for the Staphylococcus aureus 
                     CRISPR/Cas9 system"
     repeat_region   2103..2243
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      2318..2773
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3055..3159
                     /label=AmpR promoter
     CDS             3160..4017
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4191..4779
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggcctcta gactcgaggc gttgacattg attattgact agttattaat
      181 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      241 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      301 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      361 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
      421 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
      481 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      541 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      601 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      661 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
      721 tctatataag cagagctctc tggctaacta ccggtcgcca ccatggtgag caagggcgag
      781 gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc
      841 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc
      901 cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg
      961 tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac
     1021 tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac
     1081 ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag
     1141 gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg
     1201 ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc
     1261 aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac
     1321 aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc
     1381 acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac
     1441 tccaccggcg gcatggacga gctgtacaag tccggactca gatctcgagc tcaagcttcg
     1501 aattcctaga gctcgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
     1561 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     1621 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     1681 ggtggggcag gacagcaagg gggaggattg ggaagagaat agcaggcatg ctggggaggt
     1741 acctgagggc ctatttccca tgattccttc atatttgcat atacgataca aggctgttag
     1801 agagataatt ggaattaatt tgactgtaaa cacaaagata ttagtacaaa atacgtgacg
     1861 tagaaagtaa taatttcttg ggtagtttgc agttttaaaa ttatgtttta aaatggacta
     1921 tcatatgctt accgtaactt gaaagtattt cgatttcttg gctttatata tcttgtggaa
     1981 aggacgaaac accggagacc acggcaggtc tcagttttag tactctggaa acagaatcta
     2041 ctaaaacaag gcaaaatgcc gtgtttatct cgtcaacttg ttggcgagat ttttgcggcc
     2101 gcaggaaccc ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga
     2161 ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct cagtgagcga
     2221 gcgagcgcgc agctgcctgc aggggcgcct gatgcggtat tttctcctta cgcatctgtg
     2281 cggtatttca caccgcatac gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta
     2341 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     2401 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     2461 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     2521 aaaaaacttg atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     2581 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     2641 acactcaacc ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggcc
     2701 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     2761 acgtttacaa ttttatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     2821 cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
     2881 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
     2941 tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     3001 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     3061 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     3121 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     3181 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     3241 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     3301 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     3361 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     3421 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     3481 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     3541 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     3601 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     3661 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     3721 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     3781 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     3841 tttattgctg ataaatctgg agccggtgag cgtggaagcc gcggtatcat tgcagcactg
     3901 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     3961 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     4021 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     4081 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     4141 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     4201 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     4261 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     4321 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
     4381 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     4441 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     4501 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     4561 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     4621 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     4681 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     4741 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     4801 ttacggttcc tggccttttg ctggcctttt gctcacatgt
//
