LOCUS       V032490                 6593 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V032490
VERSION     V032490
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     repeat_region   1..141
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     enhancer        156..535
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        536..739
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             770..1486
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK"
                     /label="EGFP"
     CDS             1523..2692
                     /label="Tgfb1(NM_021578)"
                     /note="Tgfb1(NM_021578)"
                     /gene="Tgfb1"
     misc_feature    2720..3308
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     polyA_signal    3340..3816
                     /note="human growth hormone polyadenylation signal"
                     /label="hGH poly(A) signal"
     repeat_region   3856..3996
                     /note="inverted terminal repeat of adeno-associated virus
                     serotype 2"
                     /rpt_type=inverted
                     /label="AAV2 ITR"
     rep_origin      4071..4526
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4808..4912
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             4913..5773
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      5944..6532
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtgacat tgattattga ctagttatta
      181 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
      241 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
      301 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
      361 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
      421 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
      481 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat
      541 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag
      601 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc
      661 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga
      721 ggtctatata agcagagctg gtaccggatc ctctagagtc gacgccacca tggtgagcaa
      781 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
      841 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
      901 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
      961 cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt
     1021 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
     1081 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
     1141 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
     1201 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt
     1261 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
     1321 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac
     1381 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
     1441 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagggcg gtggaggcag
     1501 tggtggaggc ggtagcgagc tcatgccgcc ctcggggctg cggctgctgc cgcttctgct
     1561 cccactcccg tggcttctag tgctgacgcc cgggaggcca gccgcgggac tctccacctg
     1621 caagaccatc gacatggagc tggtgaaacg gaagcgcatc gaagccatcc gtggccagat
     1681 cctgtccaaa ctaaggctcg ccagtccccc gagccagggg gaggtaccgc cgggcccgct
     1741 gcccgaggcg gtgctcgctt tgtacaacag cacccgcgac cgggtggcag gcgagagcgc
     1801 tgacccggag cccgagcccg aggcggacta ctacgccaaa gaagtcaccc gcgtgctaat
     1861 ggtggaccgc aacaacgcaa tctatgacaa aaccaaagac atcacacaca gtatatatat
     1921 gttcttcaat acgtcagaca ttcgggaagc agtgccagaa cccccattgc tgtcccgtgc
     1981 agagctgcgc ctgcagagat tcaagtcaac tgtggagcaa cacgtagaac tctaccagaa
     2041 atatagcaac aattcctggc gttaccttgg taaccggctg ctgaccccca ctgatacgcc
     2101 tgagtggctg tcttttgacg tcactggagt tgtccggcag tggctgaacc aaggagacgg
     2161 aatacagggc tttcgcttca gtgctcactg ctcttgtgac agcaaagata atgtactcca
     2221 cgtggaaatc aatgggatca gtcccaaacg tcgaggtgac ctgggcacca tccatgacat
     2281 gaaccgaccc ttcctgctcc tcatggccac ccccctggaa agggctcaac acctgcacag
     2341 ctccaggcac cggagagccc tggataccaa ctactgcttc agctccacag agaagaactg
     2401 ctgtgtacgg cagctgtaca ttgactttag gaaggacctg ggttggaagt ggatccacga
     2461 gcccaagggc taccatgcca acttctgtct ggggccctgc ccctacattt ggagcctgga
     2521 cacacagtac agcaaggtcc ttgccctcta caaccaacac aacccgggtg cttccgcatc
     2581 accgtgctgc gtgccgcagg ctttggagcc actgcccatc gtctactacg tgggtcgcaa
     2641 gcccaaggtg gagcagttgt ccaacatgat cgtgcgctcc tgcaagtgca gctgagaatt
     2701 cgatatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg
     2761 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     2821 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     2881 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     2941 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     3001 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     3061 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
     3121 gtcctttcct tggctgctcg cctatgttgc cacctggatt ctgcgcggga cgtccttctg
     3181 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     3241 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     3301 ctccccgcat cgataccgag cgctgctcga gagatctacg ggtggcatcc ctgtgacccc
     3361 tccccagtgc ctctcctggc cctggaagtt gccactccag tgcccaccag ccttgtccta
     3421 ataaaattaa gttgcatcat tttgtctgac taggtgtcct tctataatat tatggggtgg
     3481 aggggggtgg tatggagcaa ggggcaagtt gggaagacaa cctgtagggc ctgcggggtc
     3541 tattgggaac caagctggag tgcagtggca caatcttggc tcactgcaat ctccgcctcc
     3601 tgggttcaag cgattctcct gcctcagcct cccgagttgt tgggattcca ggcatgcatg
     3661 accaggctca gctaattttt gtttttttgg tagagacggg gtttcaccat attggccagg
     3721 ctggtctcca actcctaatc tcaggtgatc tacccacctt ggcctcccaa attgctggga
     3781 ttacaggcgt gaaccactgc tcccttccct gtccttctga ttttgtaggt aaccacgtgc
     3841 ggaccgagcg gccgcaggaa cccctagtga tggagttggc cactccctct ctgcgcgctc
     3901 gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg
     3961 cctcagtgag cgagcgagcg cgcagctgcc tgcaggggcg cctgatgcgg tattttctcc
     4021 ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag caaccatagt acgcgccctg
     4081 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     4141 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     4201 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     4261 gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc catcgccctg
     4321 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     4381 ccaaactgga acaacactca accctatctc gggctattct tttgatttat aagggatttt
     4441 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     4501 taacaaaata ttaacgttta caattttatg gtgcactctc agtacaatct gctctgatgc
     4561 cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
     4621 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
     4681 gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt
     4741 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     4801 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     4861 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     4921 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     4981 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     5041 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     5101 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     5161 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     5221 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     5281 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     5341 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     5401 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     5461 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     5521 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     5581 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     5641 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     5701 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     5761 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     5821 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     5881 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     5941 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     6001 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     6061 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
     6121 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     6181 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     6241 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     6301 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     6361 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     6421 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     6481 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     6541 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgt
//
