LOCUS V045972 5862 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V045972 VERSION V045972 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers repeat_region 1..141 /note="inverted terminal repeat of adeno-associated virus serotype 2" /rpt_type=inverted /label="AAV2 ITR" enhancer 156..535 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 536..739 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 771..1478 /codon_start=1 /note="mammalian codon-optimized" /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /transl_table=1 /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" /label="mCherry" CDS 1479..1973 /label="Gadd45a(NM_007836)" /note="Gadd45a(NM_007836)" /gene="Gadd45a" misc_feature 1989..2577 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" polyA_signal 2609..3085 /note="human growth hormone polyadenylation signal" /label="hGH poly(A) signal" repeat_region 3125..3265 /note="inverted terminal repeat of adeno-associated virus serotype 2" /rpt_type=inverted /label="AAV2 ITR" rep_origin 3340..3795 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 4077..4181 /gene="bla" /label="AmpR promoter" CDS 4182..5042 /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" rep_origin 5213..5801 /direction=RIGHT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" ORIGIN 1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 121 actccatcac taggggttcc tgcggccgca cgcgtgacat tgattattga ctagttatta 181 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata 241 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat 301 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga 361 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc 421 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt 481 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat 541 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag 601 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc 661 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga 721 ggtctatata agcagagctg gtaccggatc ctctagagtc gacagccacc atggtgagca 781 agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg 841 agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg 901 agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg 961 acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca 1021 tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact 1081 tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca 1141 tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga 1201 agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg 1261 gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga 1321 ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt 1381 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg 1441 gccgccactc caccggcggc atggacgagc tgtacaagat gactttggag gaattctcgg 1501 ctgcagagca gaagaccgaa aggatggaca cggtgggcga tgccctggag gaagtgctca 1561 gcaaggctcg gagtcagcgc accattacgg tcggcgtgta cgaggctgcc aagctgctca 1621 acgtagaccc cgataacgtg gtactgtgcc tgctggctgc tgacgaagac gacgaccggg 1681 atgtggctct gcagatccat ttcaccctca tccgtgcgtt ctgctgcgag aacgacatca 1741 acatcctgcg ggtcagcaac ccgggtcggc tagctgagct gctgctactg gagaacgacg 1801 cgggcccggc ggagagcggg ggcgccgcgc agaccccgga cctgcactgt gtgctggtga 1861 cgaacccaca ttcatcacaa tggaaggatc ctgccttaag tcaacttatt tgtttttgcc 1921 gggaaagtcg ctacatggat cagtgggtgc ccgtgattaa tctcccggaa cggtgaaagc 1981 ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact 2041 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg 2101 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg 2161 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa 2221 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc 2281 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg 2341 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt 2401 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt 2461 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc 2521 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc 2581 gataccgagc gctgctcgag agatctacgg gtggcatccc tgtgacccct ccccagtgcc 2641 tctcctggcc ctggaagttg ccactccagt gcccaccagc cttgtcctaa taaaattaag 2701 ttgcatcatt ttgtctgact aggtgtcctt ctataatatt atggggtgga ggggggtggt 2761 atggagcaag gggcaagttg ggaagacaac ctgtagggcc tgcggggtct attgggaacc 2821 aagctggagt gcagtggcac aatcttggct cactgcaatc tccgcctcct gggttcaagc 2881 gattctcctg cctcagcctc ccgagttgtt gggattccag gcatgcatga ccaggctcag 2941 ctaatttttg tttttttggt agagacgggg tttcaccata ttggccaggc tggtctccaa 3001 ctcctaatct caggtgatct acccaccttg gcctcccaaa ttgctgggat tacaggcgtg 3061 aaccactgct cccttccctg tccttctgat tttgtaggta accacgtgcg gaccgagcgg 3121 ccgcaggaac ccctagtgat ggagttggcc actccctctc tgcgcgctcg ctcgctcact 3181 gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc 3241 gagcgagcgc gcagctgcct gcaggggcgc ctgatgcggt attttctcct tacgcatctg 3301 tgcggtattt cacaccgcat acgtcaaagc aaccatagta cgcgccctgt agcggcgcat 3361 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag 3421 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc 3481 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc 3541 ccaaaaaact tgatttgggt gatggttcac gtagtgggcc atcgccctga tagacggttt 3601 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa 3661 caacactcaa ccctatctcg ggctattctt ttgatttata agggattttg ccgatttcgg 3721 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat 3781 taacgtttac aattttatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa 3841 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg 3901 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac 3961 cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta 4021 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg 4081 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat 4141 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc 4201 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa 4261 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac 4321 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga 4381 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag 4441 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca 4501 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca 4561 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa 4621 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc 4681 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa 4741 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag 4801 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct 4861 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac 4921 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa 4981 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt 5041 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat 5101 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg 5161 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc 5221 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg 5281 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag 5341 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact 5401 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg 5461 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc 5521 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg 5581 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg 5641 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag 5701 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc 5761 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct 5821 ttttacggtt cctggccttt tgctggcctt ttgctcacat gt //