LOCUS V022401 8514 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V022401 VERSION V022401 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers repeat_region 1..141 /note="inverted terminal repeat of adeno-associated virus serotype 2" /rpt_type=inverted /label="AAV2 ITR" enhancer 221..524 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 525..727 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" intron 876..1254 /note="fusion between human cytomegalovirus intron A and human β-globin intron 2" /label="chimeric intron" CDS 1338..2045 /codon_start=1 /note="mammalian codon-optimized" /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /transl_table=1 /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" /label="mCherry" CDS 2046..2102 /codon_start=1 /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /product="2A peptide from porcine teschovirus-1 polyprotein" /transl_table=1 /translation="ATNFSLLKQAGDVEENPGP" /label="P2A" CDS 2109..5198 /label="CDKL5(NM_001037343)" /note="CDKL5(NM_001037343)" /gene="CDKL5" CDS 5199..5222 /codon_start=1 /product="FLAG® epitope tag, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDDDDK" /label="FLAG" polyA_signal 5261..5737 /note="human growth hormone polyadenylation signal" /label="hGH poly(A) signal" repeat_region 5777..5917 /note="inverted terminal repeat of adeno-associated virus serotype 2" /rpt_type=inverted /label="AAV2 ITR" rep_origin 5992..6447 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 6729..6833 /gene="bla" /label="AmpR promoter" CDS 6834..7694 /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" rep_origin 7865..8453 /direction=RIGHT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" ORIGIN 1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 121 actccatcac taggggttcc tgcggccgca cgcgtggagc tagttattaa tagtaatcaa 181 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa 241 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 301 ttcccatagt aacgtcaata gggactttcc attgacgtca atgggtggag tatttacggt 361 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 421 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 481 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 541 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 601 ttgacgtcaa tgggagtttg ttttgcacca aaatcaacgg gactttccaa aatgtcgtaa 661 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag 721 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct 781 ccatagaaga caccgggacc gatccagcct ccgcggattc gaatcccggc cgggaacggt 841 gcattggaac gcggattccc cgtgccaaga gtgacgtaag taccgcctat agagtctata 901 ggcccacaaa aaatgctttc ttcttttaat atactttttt gtttatctta tttctaatac 961 tttccctaat ctctttcttt cagggcaata atgatacaat gtatcatgcc tctttgcacc 1021 attctaaaga ataacagtga taatttctgg gttaaggcaa tagcaatatt tctgcatata 1081 aatatttctg catataaatt gtaactgatg taagaggttt catattgcta atagcagcta 1141 caatccagct accattctgc ttttatttta tggttgggat aaggctggat tattctgagt 1201 ccaagctagg cccttttgct aatcatgttc atacctctta tcttcctccc acagctcctg 1261 ggcaacgtgc tggtctgtgt gctggcccat cactttggca aagaattggg attcgaacat 1321 cgattgaatt cgccaccatg gtgagcaagg gcgaggagga taacatggcc atcatcaagg 1381 agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg 1441 agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca 1501 agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca 1561 aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg 1621 gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg 1681 actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc 1741 cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga 1801 tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg 1861 gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc 1921 ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca 1981 tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt 2041 acaaggccac gaacttctct ctgttaaagc aagcaggaga cgtggaagaa aaccccggtc 2101 ctggatccat gaagattcct aacattggta atgtgatgaa taaatttgag atccttgggg 2161 ttgtaggtga aggagcctat ggagttgtac ttaaatgcag acacaaggaa acacatgaaa 2221 ttgtggcgat caagaaattc aaggacagtg aagaaaatga agaagtcaaa gaaacgactt 2281 tacgagagct taaaatgctt cggactctca agcaggaaaa cattgtggag ttgaaggaag 2341 catttcgtcg gaggggaaag ttgtacttgg tgtttgagta tgttgaaaaa aatatgctcg 2401 aattgctgga agaaatgcca aatggagttc cacctgagaa agtaaaaagc tacatctatc 2461 agctaatcaa ggctattcac tggtgccata agaatgatat tgtccatcga gatataaaac 2521 cagaaaatct cttaatcagc cacaatgatg tcctaaaact gtgtgacttt ggttttgctc 2581 gtaatctgtc agaaggcaat aatgctaatt acacagagta cgttgccacc agatggtatc 2641 ggtccccaga actcttactt ggcgctccct atggaaagtc cgtggacatg tggtcggtgg 2701 gctgtattct tggggagctt agcgatggac agcctttatt tcctggagaa agtgaaattg 2761 accaactttt tactattcag aaggtgctag gaccacttcc atctgagcag atgaagcttt 2821 tctacagtaa tcctcgcttc catgggctcc ggtttccagc tgttaaccat cctcagtcct 2881 tggaaagaag ataccttgga attttgaata gtgttctact tgacctaatg aagaatttac 2941 tgaagttgga cccagctgac agatacttga cagaacagtg tttgaatcac cctacatttc 3001 aaacccagag acttctggat cgttctcctt caaggtcagc aaaaagaaaa ccttaccatg 3061 tggaaagcag cacattgtct aatagaaacc aagccggcaa aagtactgct ttgcagtctc 3121 accacagatc taacagcaag gacatccaga acctgagtgt aggcctgccc cgggctgacg 3181 aaggtctccc tgccaatgaa agcttcctaa atggaaacct tgctggagct agtcttagtc 3241 cactgcacac caaaacctac caagcaagca gccagcctgg gtctaccagc aaagatctca 3301 ccaacaacaa cataccacac cttcttagcc caaaagaagc caagtcaaaa acagagtttg 3361 attttaatat tgacccaaag ccttcagaag gcccagggac aaagtacctc aagtcaaaca 3421 gcagatctca gcagaaccgc cactcattca tggaaagctc tcaaagcaaa gctgggacac 3481 tgcagcccaa tgaaaagcag agtcggcata gctatattga cacaattccc cagtcctcta 3541 ggagtccctc ctacaggacc aaggccaaaa gccatggggc actgagtgac tccaagtctg 3601 tgagcaacct ttctgaagcc agggcccaaa ttgcggagcc cagtaccagt aggtacttcc 3661 catctagctg cttagacttg aattctccca ccagcccaac ccccaccaga cacagtgaca 3721 cgagaacttt gctcagccct tctggaagaa ataaccgaaa tgagggaacg ctggactcac 3781 gtcgaaccac aaccagacat tctaagacga tggaggaatt gaagctgccg gagcacatgg 3841 acagtagcca ttcccattca ctgtctgcac ctcacgaatc tttttcttat ggactgggct 3901 acaccagccc cttttcttcc cagcaacgtc ctcataggca ttctatgtat gtgacccgtg 3961 acaaagtgag agccaagggc ttggatggaa gcttgagcat agggcaaggg atggcagcta 4021 gagccaacag cctgcaactc ttgtcacccc agcctggaga acagctccct ccagagatga 4081 ctgtggcaag atcttcggtc aaagagacct ccagagaagg cacctcttcc ttccatacac 4141 gccagaagtc tgagggtgga gtgtatcatg acccacactc tgatgatggc acagccccca 4201 aagaaaatag acacctatac aatgatcctg tgccaaggag agttggtagc ttttacagag 4261 tgccatctcc acgtccagac aattctttcc atgaaaataa tgtgtcaact agagtttctt 4321 ctctaccatc agagagcagt tctggaacca accactcaaa aagacaacca gcattcgatc 4381 catggaaaag tcctgaaaat attagtcatt cagagcaact caaggaaaaa gagaagcaag 4441 gatttttcag gtcaatgaaa aagaaaaaga agaaatctca aacagtaccc aattccgaca 4501 gccctgatct tctgacgttg cagaaatcca ttcattctgc tagcactcca agcagcagac 4561 caaaggagtg gcgccccgag aagatctcag atctgcagac ccaaagccag ccattaaaat 4621 cactgcgcaa gttgttacat ctctcttcgg cctcaaatca cccggcttcc tcagatcccc 4681 gcttccagcc cttaacagct caacaaacca aaaattcctt ctcagaaatt cggattcacc 4741 ccctgagcca ggcctctggc gggagcagca acatccggca ggaacccgca ccgaagggca 4801 ggccagccct ccagctgcca gacggtggat gtgatggcag aagacagaga caccattctg 4861 gaccccaaga tagacgcttc atgttaagga cgacagaaca acaaggagaa tacttctgct 4921 gtggtgaccc aaagaagcct cacactccgt gcgtcccaaa ccgagccctt catcgtccaa 4981 tctccagtcc tgctccctat ccagtactcc aggtccgagg cacttccatg tgcccgacac 5041 tccaggtccg aggcactgat gctttcagct gcccaaccca gcaatccggg ttctctttct 5101 tcgtgagaca cgttatgagg gaagccctga ttcacagggc ccaggtaaac caagctgcgc 5161 tcctgacata ccatgagaat gcggcactga cgggcaagga ttacaaggac gacgatgaca 5221 agtgaagctt gcctcgagca gcgctgctcg agagatctac gggtggcatc cctgtgaccc 5281 ctccccagtg cctctcctgg ccctggaagt tgccactcca gtgcccacca gccttgtcct 5341 aataaaatta agttgcatca ttttgtctga ctaggtgtcc ttctataata ttatggggtg 5401 gaggggggtg gtatggagca aggggcaagt tgggaagaca acctgtaggg cctgcggggt 5461 ctattgggaa ccaagctgga gtgcagtggc acaatcttgg ctcactgcaa tctccgcctc 5521 ctgggttcaa gcgattctcc tgcctcagcc tcccgagttg ttgggattcc aggcatgcat 5581 gaccaggctc agctaatttt tgtttttttg gtagagacgg ggtttcacca tattggccag 5641 gctggtctcc aactcctaat ctcaggtgat ctacccacct tggcctccca aattgctggg 5701 attacaggcg tgaaccactg ctcccttccc tgtccttctg attttgtagg taaccacgtg 5761 cggaccgagc ggccgcagga acccctagtg atggagttgg ccactccctc tctgcgcgct 5821 cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg 5881 gcctcagtga gcgagcgagc gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc 5941 cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct 6001 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg 6061 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg 6121 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac 6181 ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct 6241 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt 6301 tccaaactgg aacaacactc aaccctatct cgggctattc ttttgattta taagggattt 6361 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt 6421 ttaacaaaat attaacgttt acaattttat ggtgcactct cagtacaatc tgctctgatg 6481 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt 6541 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc 6601 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat 6661 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg 6721 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc 6781 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta 6841 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg 6901 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg 6961 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac 7021 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg 7081 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt 7141 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg 7201 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac 7261 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt 7321 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag 7381 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc 7441 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc 7501 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta 7561 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg 7621 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga 7681 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac 7741 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa 7801 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat 7861 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc 7921 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg 7981 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc 8041 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg 8101 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg 8161 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa 8221 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg 8281 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga 8341 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct 8401 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca 8461 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgt //