LOCUS       40924_3022        7034 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pAAV-9(5)hSyn-EGFP-NRSEdsRNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7034)
  AUTHORS   Koch JC, Barski E, Lingor P, Bahr M, Michel U.
  TITLE     Plasmids containing NRSE/RE1 sites enhance neurite outgrowth of 
            retinal ganglion cells via sequestration of REST independent of NRSE
            dsRNA expression
  JOURNAL   FEBS J. 278 (18), 3472-3483 (2011)
  PUBMED    21790997
REFERENCE   2  (bases 1 to 7034)
  AUTHORS   Koch JC, Michel U.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-OCT-2010) Neurology, University Medicine Goettingen, 
            Robert-Koch-Str. 40, Goettingen, Niedersachsen 37075, Germany
REFERENCE   3  (bases 1 to 7034)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7034)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEBS J."; 
            date: "2011"; volume: "278"; issue: "18"; pages: "3472-3483"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-OCT-2010) Neurology, University Medicine Goettingen, 
            Robert-Koch-Str. 40, Goettingen, Niedersachsen 37075, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7034
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    complement(51..185)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     intron          complement(201..334)
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     CDS             complement(349..1065)
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     promoter        complement(1108..1555)
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     promoter        1583..1797
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     misc_feature    1799..1866
                     /label=NRSE double-stranded RNA
                     /note="NRSE double-stranded RNA"
     misc_feature    1871..4134
                     /label=partial sequence of porcine non-coding RNA 9(5)
                     /note="partial sequence of porcine non-coding RNA 9(5)"
     repeat_region   4154..4294
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4369..4824
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5106..5210
                     /label=AmpR promoter
     CDS             5211..6068
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6242..6830
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     repeat_region   6892..7032
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
ORIGIN
        1 ggccgcacgc gatcgaattc ctgcagcccg ggggatcttc gatgctagac gatccagaca
       61 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
      121 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
      181 aagttgctcg aagtcgacac ctgtggagag aaaggcaaag tggatgtcag taagaccaat
      241 aggtgcctat cagaaacgca agagtcttct ctgtctcgac aagcccagtt tctatgtggt
      301 ctccttaaac ctgtcttgta accttgatac ttacctgccg ccgctttact tgtacagctc
      361 gtccatgccg agagtgatcc cggcggcggt cacgaactcc agcaggacca tgtgatcgcg
      421 cttctcgttg gggtctttgc tcagggcgga ctgggtgctc aggtagtggt tgtcgggcag
      481 cagcacgggg ccgtcgccga tgggggtgtt ctgctggtag tggtcggcga gctgcacgct
      541 gccgtcctcg atgttgtggc ggatcttgaa gttcaccttg atgccgttct tctgcttgtc
      601 ggccatgata tagacgttgt ggctgttgta gttgtactcc agcttgtgcc ccaggatgtt
      661 gccgtcctcc ttgaagtcga tgcccttcag ctcgatgcgg ttcaccaggg tgtcgccctc
      721 gaacttcacc tcggcgcggg tcttgtagtt gccgtcgtcc ttgaagaaga tggtgcgctc
      781 ctggacgtag ccttcgggca tggcggactt gaagaagtcg tgctgcttca tgtggtcggg
      841 gtagcggctg aagcactgca cgccgtaggt cagggtggtc acgagggtgg gccagggcac
      901 gggcagcttg ccggtggtgc agatgaactt cagggtcagc ttgccgtagg tggcatcgcc
      961 ctcgccctcg ccggacacgc tgaacttgtg gccgtttacg tcgccgtcca gctcgaccag
     1021 gatgggcacc accccggtga acagctcctc gcccttgctc accatggtgg cgaccggtgg
     1081 atccttgcta gcagcttgaa ttctcgactg cgctctcagg cacgacacga ctcctccgct
     1141 gcccaccgca gactgaggca gcgctgagtc gccggcgccg cagcgcagat ggtcgcgccc
     1201 gtgcccccct atctcgcgcc tcgcgtggtg cggtccggct gggccggcgg cggcgcggac
     1261 gcgaccaagg tggccgggaa ggggagtttg cgggggaccg gcgagtgacg tcagcgcgcc
     1321 ttcagtgctg aggcggcggt ggcgcgcgcc gccaggcggg ggcgaaggca ctgtccgcgg
     1381 tgctgaagct ggcagtgcgc acgcgcctcg ccgcatcctg tttcccctcc ccctctctga
     1441 taggggatgc gcaatttggg gaatgggggt tgggtgcttg tccagtgggt cggggtcggt
     1501 cgtcaggtag gcacccccac cccgcctcat cctggtccta aaacccactt gcactcatac
     1561 gcagggccct ctgcaggaat tcgaacgctg acgtcatcaa cccgctccaa ggaatcgcgg
     1621 gcccagtgtc actaggcggg aacacccagc gcgcgtgcgc cctggcagga agatggctgt
     1681 gagggacagg ggagtggcgc cctgcaatat ttgcatgtcg ctatgtgttc tgggaaatca
     1741 ccataaacgt gaaatgtctt tggatttggg aatcttataa gttctgtatg agaccacaga
     1801 tccccttcag caccacggac agcgcccttc ctgtcaggcg ctgtccgtgg tgctgaattt
     1861 ttggaaagct ttagtttctt catctgtaaa atagaaatga taatggtgta tacgtcatag
     1921 gactgttgtg agatttaaat gattcatgca tgaaatttct tagaagcatg cctcacatta
     1981 tagcattcat ttcactaagt gctatttttc tttccttttg actatgccca cggcatgcaa
     2041 aaattccagg gccagggatc aaacccacct gtgccacaga agtgggaatg ctggatcctt
     2101 aatccgggag ccaccagaaa acaccagtgt tgactattat cattacacac cagccagttg
     2161 tatttgtata tgactgattt ctagaatgat ggaatctcac atcataataa cctgaggatt
     2221 tacttgttac ctcaagtggg aagttcaagg atgcacctga ttctttggga ggctccatca
     2281 gtggggccag gggttctctg agttttctgt caggctccct ccaggtggta aaatggcagc
     2341 tggcagcctc atatcaacat cccccgatca attcctgtgg acaacaacca tcttttccac
     2401 ctactgtggc aaaaagtaac caaggaagac actgattgac ccagtgaggg gcacatacca
     2461 atccctgtgg cttgagggtg gagaggtgac aggtcagctt tattgtattc acaaccagga
     2521 agcagagaag ggaacaacaa atgaccacta tagttcctac gccctttcta tatgctgcag
     2581 ctgagtccat ctggcttcct gtttccattg caaaggccca ctgagttttc agctcaccat
     2641 cctctctgac agtccaagtc agagcctgac ataattggcc cccattctat aatgaatgca
     2701 ccataattag cctaggttag taaaatgcaa caagtaaaat acactcagtg cggcaatttt
     2761 tataaatctt tatccctatg gctatttcaa attcagaaga ccagactagt ccaataacta
     2821 aaagccccac acatatgtgg taaaaccatc ctctgataga tgtaacatta cacagtgggg
     2881 ccctgcagtc cacagtgaaa gagaaatagg tgaggccaga acatacactt tcttgtttca
     2941 gctctactaa tagttcaaga aaataaagtc tcttagtctc acagatacta aagatcccgt
     3001 atttctaaaa cagaggttga agcatccagg taaccgacta catggaattt ttgtgaagag
     3061 taaaatgaac tgtcatatgt agaagagatt agtaaatata ataaaaataa ttagcaatat
     3121 tatctctaat aattaaattg ttctttgttt cctaatcctc ttaaacacag aaatgatttc
     3181 ttctctttgg cttagatttc tcttctgttc tcagaatagt tgtcacttga caccctgttg
     3241 tcacctctta tacaaagaac tccaccttct cttgtctttt ctgaaacaac catgcatttg
     3301 ctttctctat ttgtcacact catctccctt ttttctctct tagatttttt tttgtttcca
     3361 ttttaattgg tcattatccc ctcactggtg ataaaagccc acccttcttt tttattcacc
     3421 atggaaccac tgtagtatgc tggttggacc attcaaatct tgacttcacc ttggaccttt
     3481 cacttgattc tgtctattca ccatcgttgc acaggagagt tctgtctgtg ttttgtttct
     3541 gagtctggca ccactctgaa ggtctaactt tgtagggagt ctctctccag ataatttaaa
     3601 aagttgaatt atccctagga agtagctcaa gaacttgaat agcctctcaa attccactca
     3661 ctggattaat gcttaaaaaa ataatttttg tggctcagtg gaattgaatc tgactagtat
     3721 ccatgaggat tcaggttcaa tccctagcct tgctcagtgg gtccaggatc cggtgttgct
     3781 gtgagctata gtgtaggttg cagacacagc ctggattctg tgttgctgtg gtataggctg
     3841 gcagctatag cactgattca accctctagg aggaactttc atatgtcgtg tgtgtggccc
     3901 cccaaaaata acaacaacga caactaaaac aataaagtaa aatgtgcttt aataaaaata
     3961 ataaataagg cattagcctg aggagctgat ggcaccacct gccttcagta caaggattca
     4021 acaccaacgt gtgggtgttt gggaagagtt gatcagaccg acactgaagc tggaaataag
     4081 cagcagcagc agtagtacca caaagcctgg gaaacacacc cacaaaatga cagtgtgcgg
     4141 accgagcggc cgcaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc
     4201 tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc
     4261 tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc tgatgcggta ttttctcctt
     4321 acgcatctgt gcggtatttc acaccgcata cgtcaaagca accatagtac gcgccctgta
     4381 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     4441 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     4501 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     4561 acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat
     4621 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     4681 aaactggaac aacactcaac cctatctcgg gctattcttt tgatttataa gggattttgc
     4741 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     4801 acaaaatatt aacgtttaca attttatggt gcactctcag tacaatctgc tctgatgccg
     4861 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     4921 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     4981 ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt
     5041 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
     5101 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     5161 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     5221 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     5281 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     5341 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     5401 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     5461 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     5521 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     5581 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     5641 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     5701 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     5761 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     5821 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     5881 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     5941 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     6001 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     6061 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     6121 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     6181 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     6241 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     6301 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     6361 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     6421 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     6481 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     6541 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     6601 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     6661 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     6721 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     6781 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     6841 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg tcctgcaggc
     6901 agctgcgcgc tcgctcgctc actgaggccg cccgggcaaa gcccgggcgt cgggcgacct
     6961 ttggtcgccc ggcctcagtg agcgagcgag cgcgcagaga gggagtggcc aactccatca
     7021 ctaggggttc ctgc
//
