LOCUS       40924_3017        6994 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pAAV-9(5)hSyn-DsRed2N1-NRSEdsRNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6994)
  AUTHORS   Koch JC, Barski E, Lingor P, Bahr M, Michel U.
  TITLE     Plasmids containing NRSE/RE1 sites enhance neurite outgrowth of 
            retinal ganglion cells via sequestration of REST independent of NRSE
            dsRNA expression
  JOURNAL   FEBS J. 278 (18), 3472-3483 (2011)
  PUBMED    21790997
REFERENCE   2  (bases 1 to 6994)
  AUTHORS   Koch JC, Michel U.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-OCT-2010) Neurology, University Medicine Goettingen, 
            Robert-Koch-Str. 40, Goettingen, Niedersachsen 37075, Germany
REFERENCE   3  (bases 1 to 6994)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6994)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEBS J."; 
            date: "2011"; volume: "278"; issue: "18"; pages: "3472-3483"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-OCT-2010) Neurology, University Medicine Goettingen, 
            Robert-Koch-Str. 40, Goettingen, Niedersachsen 37075, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6994
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     polyA_signal    complement(194..328)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     intron          complement(344..477)
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     CDS             complement(494..1168)
                     /label=DsRed2
                     /note="improved tetrameric variant of DsRed fluorescent
                     protein"
     promoter        complement(1211..1658)
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      1674..1685
                     /label=human H1 promoter (H1)
                     /note="human H1 promoter (H1)"
                     /regulatory_class="promoter"
     promoter        1686..1900
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     misc_feature    1902..1969
                     /label=NRSE double-stranded RNA
                     /note="NRSE double-stranded RNA"
     misc_feature    1974..4237
                     /label=partial sequence of porcine non-coding RNA 9(5)
                     /note="partial sequence of porcine non-coding RNA 9(5)"
     repeat_region   4257..4397
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4472..4927
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5209..5313
                     /label=AmpR promoter
     CDS             5314..6171
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      6345..6933
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgatcgaa ttcctgcagc ccgggggatc
      181 ttcgatgcta gacgatccag acatgataag atacattgat gagtttggac aaaccacaac
      241 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
      301 aaccattata agctgcaata aacaagttgc tcgaagtcga cacctgtgga gagaaaggca
      361 aagtggatgt cagtaagacc aataggtgcc tatcagaaac gcaagagtct tctctgtctc
      421 gacaagccca gtttctatgt ggtctcctta aacctgtctt gtaaccttga tacttacctg
      481 cggccggccg ctacaggaac aggtggtggc ggccctcggt gcgctcgtac tgctccacga
      541 tggtgtagtc ctcgttgtgg gaggtgatgt ccagcttggc gtccacgtag tagtagccgg
      601 gcagctgcac gggcttcttg gccatgtaga tggacttgaa ctccaccagg tagtggccgc
      661 cgtccttcag cttcagggcc ttgtgggtct cgcccttcag cacgccgtcg cgggggtaca
      721 ggcgctcggt ggaggcctcc cagcccatgg tcttcttctg catcacgggg ccgtcggagg
      781 ggaagttcac gccgatgaac ttcaccttgt agatgaagca gccgtcctgc agggaggagt
      841 cctgggtcac ggtcgccacg ccgccgtcct cgaagttcat cacgcgctcc cacttgaagc
      901 cctcggggaa ggacagcttc ttgtagtcgg ggatgtcggc ggggtgcttc acgtacacct
      961 tggagccgta ctggaactgg ggggacagga tgtcccaggc gaagggcagg gggccgccct
     1021 tggtcacctt cagcttcacg gtgttgtggc cctcgtaggg gcggccctcg ccctcgccct
     1081 cgatctcgaa ctcgtggccg ttcacggtgc cctccatgcg caccttgaag cgcatgaact
     1141 cggtgatgac gttctcggag gaggccatgg tggcgaccgg tggatccttg ctagcagctt
     1201 gaattctcga ctgcgctctc aggcacgaca cgactcctcc gctgcccacc gcagactgag
     1261 gcagcgctga gtcgccggcg ccgcagcgca gatggtcgcg cccgtgcccc cctatctcgc
     1321 gcctcgcgtg gtgcggtccg gctgggccgg cggcggcgcg gacgcgacca aggtggccgg
     1381 gaaggggagt ttgcggggga ccggcgagtg acgtcagcgc gccttcagtg ctgaggcggc
     1441 ggtggcgcgc gccgccaggc gggggcgaag gcactgtccg cggtgctgaa gctggcagtg
     1501 cgcacgcgcc tcgccgcatc ctgtttcccc tccccctctc tgatagggga tgcgcaattt
     1561 ggggaatggg ggttgggtgc ttgtccagtg ggtcggggtc ggtcgtcagg taggcacccc
     1621 caccccgcct catcctggtc ctaaaaccca cttgcactca tacgcagggc cctctgcagg
     1681 aattcgaacg ctgacgtcat caacccgctc caaggaatcg cgggcccagt gtcactaggc
     1741 gggaacaccc agcgcgcgtg cgccctggca ggaagatggc tgtgagggac aggggagtgg
     1801 cgccctgcaa tatttgcatg tcgctatgtg ttctgggaaa tcaccataaa cgtgaaatgt
     1861 ctttggattt gggaatctta taagttctgt atgagaccac agatcccctt cagcaccacg
     1921 gacagcgccc ttcctgtcag gcgctgtccg tggtgctgaa tttttggaaa gctttagttt
     1981 cttcatctgt aaaatagaaa tgataatggt gtatacgtca taggactgtt gtgagattta
     2041 aatgattcat gcatgaaatt tcttagaagc atgcctcaca ttatagcatt catttcacta
     2101 agtgctattt ttctttcctt ttgactatgc ccacggcatg caaaaattcc agggccaggg
     2161 atcaaaccca cctgtgccac agaagtggga atgctggatc cttaatccgg gagccaccag
     2221 aaaacaccag tgttgactat tatcattaca caccagccag ttgtatttgt atatgactga
     2281 tttctagaat gatggaatct cacatcataa taacctgagg atttacttgt tacctcaagt
     2341 gggaagttca aggatgcacc tgattctttg ggaggctcca tcagtggggc caggggttct
     2401 ctgagttttc tgtcaggctc cctccaggtg gtaaaatggc agctggcagc ctcatatcaa
     2461 catcccccga tcaattcctg tggacaacaa ccatcttttc cacctactgt ggcaaaaagt
     2521 aaccaaggaa gacactgatt gacccagtga ggggcacata ccaatccctg tggcttgagg
     2581 gtggagaggt gacaggtcag ctttattgta ttcacaacca ggaagcagag aagggaacaa
     2641 caaatgacca ctatagttcc tacgcccttt ctatatgctg cagctgagtc catctggctt
     2701 cctgtttcca ttgcaaaggc ccactgagtt ttcagctcac catcctctct gacagtccaa
     2761 gtcagagcct gacataattg gcccccattc tataatgaat gcaccataat tagcctaggt
     2821 tagtaaaatg caacaagtaa aatacactca gtgcggcaat ttttataaat ctttatccct
     2881 atggctattt caaattcaga agaccagact agtccaataa ctaaaagccc cacacatatg
     2941 tggtaaaacc atcctctgat agatgtaaca ttacacagtg gggccctgca gtccacagtg
     3001 aaagagaaat aggtgaggcc agaacataca ctttcttgtt tcagctctac taatagttca
     3061 agaaaataaa gtctcttagt ctcacagata ctaaagatcc cgtatttcta aaacagaggt
     3121 tgaagcatcc aggtaaccga ctacatggaa tttttgtgaa gagtaaaatg aactgtcata
     3181 tgtagaagag attagtaaat ataataaaaa taattagcaa tattatctct aataattaaa
     3241 ttgttctttg tttcctaatc ctcttaaaca cagaaatgat ttcttctctt tggcttagat
     3301 ttctcttctg ttctcagaat agttgtcact tgacaccctg ttgtcacctc ttatacaaag
     3361 aactccacct tctcttgtct tttctgaaac aaccatgcat ttgctttctc tatttgtcac
     3421 actcatctcc cttttttctc tcttagattt ttttttgttt ccattttaat tggtcattat
     3481 cccctcactg gtgataaaag cccacccttc ttttttattc accatggaac cactgtagta
     3541 tgctggttgg accattcaaa tcttgacttc accttggacc tttcacttga ttctgtctat
     3601 tcaccatcgt tgcacaggag agttctgtct gtgttttgtt tctgagtctg gcaccactct
     3661 gaaggtctaa ctttgtaggg agtctctctc cagataattt aaaaagttga attatcccta
     3721 ggaagtagct caagaacttg aatagcctct caaattccac tcactggatt aatgcttaaa
     3781 aaaataattt ttgtggctca gtggaattga atctgactag tatccatgag gattcaggtt
     3841 caatccctag ccttgctcag tgggtccagg atccggtgtt gctgtgagct atagtgtagg
     3901 ttgcagacac agcctggatt ctgtgttgct gtggtatagg ctggcagcta tagcactgat
     3961 tcaaccctct aggaggaact ttcatatgtc gtgtgtgtgg ccccccaaaa ataacaacaa
     4021 cgacaactaa aacaataaag taaaatgtgc tttaataaaa ataataaata aggcattagc
     4081 ctgaggagct gatggcacca cctgccttca gtacaaggat tcaacaccaa cgtgtgggtg
     4141 tttgggaaga gttgatcaga ccgacactga agctggaaat aagcagcagc agcagtagta
     4201 ccacaaagcc tgggaaacac acccacaaaa tgacagtgtg cggaccgagc ggccgcagga
     4261 acccctagtg atggagttgg ccactccctc tctgcgcgct cgctcgctca ctgaggccgg
     4321 gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc
     4381 gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     4441 ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc attaagcgcg
     4501 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     4561 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     4621 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     4681 cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     4741 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     4801 aaccctatct cgggctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     4861 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt
     4921 acaattttat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc
     4981 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
     5041 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
     5101 ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     5161 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     5221 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     5281 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     5341 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     5401 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     5461 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     5521 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     5581 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     5641 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     5701 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     5761 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     5821 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     5881 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     5941 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     6001 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     6061 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     6121 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     6181 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     6241 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     6301 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     6361 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     6421 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     6481 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     6541 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     6601 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     6661 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     6721 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     6781 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     6841 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     6901 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     6961 ttcctggcct tttgctggcc ttttgctcac atgt
//
