LOCUS       40924_3012        7028 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pAAV-9(5)4xNRSE-CytbAS-hSyn-EGFP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7028)
  AUTHORS   Koch JC, Barski E, Lingor P, Bahr M, Michel U.
  TITLE     Plasmids containing NRSE/RE1 sites enhance neurite outgrowth of 
            retinal ganglion cells via sequestration of REST independent of NRSE
            dsRNA expression
  JOURNAL   FEBS J. 278 (18), 3472-3483 (2011)
  PUBMED    21790997
REFERENCE   2  (bases 1 to 7028)
  AUTHORS   Koch JC, Michel U.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-OCT-2010) Neurology, University Medicine Goettingen, 
            Robert-Koch-Str. 40, Goettingen, Niedersachsen 37075, Germany
REFERENCE   3  (bases 1 to 7028)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7028)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEBS J."; 
            date: "2011"; volume: "278"; issue: "18"; pages: "3472-3483"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-OCT-2010) Neurology, University Medicine Goettingen, 
            Robert-Koch-Str. 40, Goettingen, Niedersachsen 37075, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7028
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    complement(51..185)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     intron          complement(201..334)
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     CDS             complement(349..1065)
                     /label=EGFP
                     /note="enhanced GFP"
     promoter        complement(1108..1555)
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     misc_feature    1610..1722
                     /note="NRSE sequence; 4 fold"
     misc_feature    1729..1860
                     /label=partial sequence of cytochrome b
                     /note="partial sequence of cytochrome b"
     misc_feature    1865..4128
                     /label=partial sequence of porcine non-coding RNA 9(5)
                     /note="partial sequence of porcine non-coding RNA 9(5)"
     repeat_region   4148..4288
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4363..4818
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5100..5204
                     /label=AmpR promoter
     CDS             5205..6062
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      6236..6824
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     repeat_region   6886..7026
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
ORIGIN
        1 ggccgcacgc gatcgaattc ctgcagcccg ggggatcttc gatgctagac gatccagaca
       61 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
      121 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
      181 aagttgctcg aagtcgacac ctgtggagag aaaggcaaag tggatgtcag taagaccaat
      241 aggtgcctat cagaaacgca agagtcttct ctgtctcgac aagcccagtt tctatgtggt
      301 ctccttaaac ctgtcttgta accttgatac ttacctgccg ccgctttact tgtacagctc
      361 gtccatgccg agagtgatcc cggcggcggt cacgaactcc agcaggacca tgtgatcgcg
      421 cttctcgttg gggtctttgc tcagggcgga ctgggtgctc aggtagtggt tgtcgggcag
      481 cagcacgggg ccgtcgccga tgggggtgtt ctgctggtag tggtcggcga gctgcacgct
      541 gccgtcctcg atgttgtggc ggatcttgaa gttcaccttg atgccgttct tctgcttgtc
      601 ggccatgata tagacgttgt ggctgttgta gttgtactcc agcttgtgcc ccaggatgtt
      661 gccgtcctcc ttgaagtcga tgcccttcag ctcgatgcgg ttcaccaggg tgtcgccctc
      721 gaacttcacc tcggcgcggg tcttgtagtt gccgtcgtcc ttgaagaaga tggtgcgctc
      781 ctggacgtag ccttcgggca tggcggactt gaagaagtcg tgctgcttca tgtggtcggg
      841 gtagcggctg aagcactgca cgccgtaggt cagggtggtc acgagggtgg gccagggcac
      901 gggcagcttg ccggtggtgc agatgaactt cagggtcagc ttgccgtagg tggcatcgcc
      961 ctcgccctcg ccggacacgc tgaacttgtg gccgtttacg tcgccgtcca gctcgaccag
     1021 gatgggcacc accccggtga acagctcctc gcccttgctc accatggtgg cgaccggtgg
     1081 atccttgcta gcagcttgaa ttctcgactg cgctctcagg cacgacacga ctcctccgct
     1141 gcccaccgca gactgaggca gcgctgagtc gccggcgccg cagcgcagat ggtcgcgccc
     1201 gtgcccccct atctcgcgcc tcgcgtggtg cggtccggct gggccggcgg cggcgcggac
     1261 gcgaccaagg tggccgggaa ggggagtttg cgggggaccg gcgagtgacg tcagcgcgcc
     1321 ttcagtgctg aggcggcggt ggcgcgcgcc gccaggcggg ggcgaaggca ctgtccgcgg
     1381 tgctgaagct ggcagtgcgc acgcgcctcg ccgcatcctg tttcccctcc ccctctctga
     1441 taggggatgc gcaatttggg gaatgggggt tgggtgcttg tccagtgggt cggggtcggt
     1501 cgtcaggtag gcacccccac cccgcctcat cctggtccta aaacccactt gcactcatac
     1561 gcagggccct ctgcagcccg ggcccgcggt accgtcgacg gatctcgaca aggcactgtc
     1621 cgcggtgctg aagctcgaca aggcactgtc cgcggtgctg aagctcgaca aggcactgtc
     1681 cgcggtgctg aagctcgaca aggcactgtc cgcggtgctg aagctcgaga tctcccaatg
     1741 tatgggatgg cggatagtaa gtttgtaatt actgtggccc ctcagaatga tatttggcct
     1801 cacgggagga catagcctat gaaggctgtt gctatagttg caagcaggag gataatgcca
     1861 agctttagtt tcttcatctg taaaatagaa atgataatgg tgtatacgtc ataggactgt
     1921 tgtgagattt aaatgattca tgcatgaaat ttcttagaag catgcctcac attatagcat
     1981 tcatttcact aagtgctatt tttctttcct tttgactatg cccacggcat gcaaaaattc
     2041 cagggccagg gatcaaaccc acctgtgcca cagaagtggg aatgctggat ccttaatccg
     2101 ggagccacca gaaaacacca gtgttgacta ttatcattac acaccagcca gttgtatttg
     2161 tatatgactg atttctagaa tgatggaatc tcacatcata ataacctgag gatttacttg
     2221 ttacctcaag tgggaagttc aaggatgcac ctgattcttt gggaggctcc atcagtgggg
     2281 ccaggggttc tctgagtttt ctgtcaggct ccctccaggt ggtaaaatgg cagctggcag
     2341 cctcatatca acatcccccg atcaattcct gtggacaaca accatctttt ccacctactg
     2401 tggcaaaaag taaccaagga agacactgat tgacccagtg aggggcacat accaatccct
     2461 gtggcttgag ggtggagagg tgacaggtca gctttattgt attcacaacc aggaagcaga
     2521 gaagggaaca acaaatgacc actatagttc ctacgccctt tctatatgct gcagctgagt
     2581 ccatctggct tcctgtttcc attgcaaagg cccactgagt tttcagctca ccatcctctc
     2641 tgacagtcca agtcagagcc tgacataatt ggcccccatt ctataatgaa tgcaccataa
     2701 ttagcctagg ttagtaaaat gcaacaagta aaatacactc agtgcggcaa tttttataaa
     2761 tctttatccc tatggctatt tcaaattcag aagaccagac tagtccaata actaaaagcc
     2821 ccacacatat gtggtaaaac catcctctga tagatgtaac attacacagt ggggccctgc
     2881 agtccacagt gaaagagaaa taggtgaggc cagaacatac actttcttgt ttcagctcta
     2941 ctaatagttc aagaaaataa agtctcttag tctcacagat actaaagatc ccgtatttct
     3001 aaaacagagg ttgaagcatc caggtaaccg actacatgga atttttgtga agagtaaaat
     3061 gaactgtcat atgtagaaga gattagtaaa tataataaaa ataattagca atattatctc
     3121 taataattaa attgttcttt gtttcctaat cctcttaaac acagaaatga tttcttctct
     3181 ttggcttaga tttctcttct gttctcagaa tagttgtcac ttgacaccct gttgtcacct
     3241 cttatacaaa gaactccacc ttctcttgtc ttttctgaaa caaccatgca tttgctttct
     3301 ctatttgtca cactcatctc ccttttttct ctcttagatt tttttttgtt tccattttaa
     3361 ttggtcatta tcccctcact ggtgataaaa gcccaccctt cttttttatt caccatggaa
     3421 ccactgtagt atgctggttg gaccattcaa atcttgactt caccttggac ctttcacttg
     3481 attctgtcta ttcaccatcg ttgcacagga gagttctgtc tgtgttttgt ttctgagtct
     3541 ggcaccactc tgaaggtcta actttgtagg gagtctctct ccagataatt taaaaagttg
     3601 aattatccct aggaagtagc tcaagaactt gaatagcctc tcaaattcca ctcactggat
     3661 taatgcttaa aaaaataatt tttgtggctc agtggaattg aatctgacta gtatccatga
     3721 ggattcaggt tcaatcccta gccttgctca gtgggtccag gatccggtgt tgctgtgagc
     3781 tatagtgtag gttgcagaca cagcctggat tctgtgttgc tgtggtatag gctggcagct
     3841 atagcactga ttcaaccctc taggaggaac tttcatatgt cgtgtgtgtg gccccccaaa
     3901 aataacaaca acgacaacta aaacaataaa gtaaaatgtg ctttaataaa aataataaat
     3961 aaggcattag cctgaggagc tgatggcacc acctgccttc agtacaagga ttcaacacca
     4021 acgtgtgggt gtttgggaag agttgatcag accgacactg aagctggaaa taagcagcag
     4081 cagcagtagt accacaaagc ctgggaaaca cacccacaaa atgacagtgt gcggaccgag
     4141 cggccgcagg aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc
     4201 actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg
     4261 agcgagcgag cgcgcagctg cctgcagggg cgcctgatgc ggtattttct ccttacgcat
     4321 ctgtgcggta tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg
     4381 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     4441 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     4501 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     4561 accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     4621 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     4681 gaacaacact caaccctatc tcgggctatt cttttgattt ataagggatt ttgccgattt
     4741 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     4801 tattaacgtt tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     4861 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     4921 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     4981 caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg
     5041 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     5101 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     5161 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     5221 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     5281 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     5341 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     5401 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     5461 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     5521 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     5581 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     5641 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     5701 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     5761 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     5821 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     5881 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     5941 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     6001 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     6061 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     6121 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     6181 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     6241 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     6301 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     6361 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     6421 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     6481 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     6541 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     6601 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     6661 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     6721 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     6781 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     6841 cctttttacg gttcctggcc ttttgctggc cttttgctca catgtcctgc aggcagctgc
     6901 gcgctcgctc gctcactgag gccgcccggg caaagcccgg gcgtcgggcg acctttggtc
     6961 gcccggcctc agtgagcgag cgagcgcgca gagagggagt ggccaactcc atcactaggg
     7021 gttcctgc
//
