LOCUS       40924_3007        7187 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pAAV-9(5)-CMV-EGFP-CytbAS, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7187)
  AUTHORS   Michel U, Muller B, Koch JC, Zhang J-N.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUL-2015) Neurology, University of Goettingen, Waldweg
            33, Goettingen, Niedersachsen 37073, Germany
REFERENCE   2  (bases 1 to 7187)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 7187)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (27-JUL-2015) Neurology, University of Goettingen, Waldweg 33, 
            Goettingen, Niedersachsen 37073, Germany"
COMMENT     SGRef: number: 2; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7187
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    complement(40..174)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     intron          complement(190..323)
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     CDS             complement(338..1054)
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     promoter        complement(1095..1623)
                     /label=mCMV promoter
                     /note="mouse cytomegalovirus (CMV) immediate early
                     promoter"
     primer_bind     1625..1641
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1661..1875
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     repeat_region   4296..4436
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4511..4966
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5248..5352
                     /label=AmpR promoter
     CDS             5353..6210
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6384..6972
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     repeat_region   7034..7174
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
ORIGIN
        1 atcgaattcc tgcagcccgg gggatcttcg atgctagacg atccagacat gataagatac
       61 attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa
      121 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agttgctcga
      181 agtcgacacc tgtggagaga aaggcaaagt ggatgtcagt aagaccaata ggtgcctatc
      241 agaaacgcaa gagtcttctc tgtctcgaca agcccagttt ctatgtggtc tccttaaacc
      301 tgtcttgtaa ccttgatact tacctgcggc cgctttactt gtacagctcg tccatgccga
      361 gagtgatccc ggcggcggtc acgaactcca gcaggaccat gtgatcgcgc ttctcgttgg
      421 ggtctttgct cagggcggac tgggtgctca ggtagtggtt gtcgggcagc agcacggggc
      481 cgtcgccgat gggggtgttc tgctggtagt ggtcggcgag ctgcacgctg ccgtcctcga
      541 tgttgtggcg gatcttgaag ttcaccttga tgccgttctt ctgcttgtcg gccatgatat
      601 agacgttgtg gctgttgtag ttgtactcca gcttgtgccc caggatgttg ccgtcctcct
      661 tgaagtcgat gcccttcagc tcgatgcggt tcaccagggt gtcgccctcg aacttcacct
      721 cggcgcgggt cttgtagttg ccgtcgtcct tgaagaagat ggtgcgctcc tggacgtagc
      781 cttcgggcat ggcggacttg aagaagtcgt gctgcttcat gtggtcgggg tagcggctga
      841 agcactgcac gccgtaggtc agggtggtca cgagggtggg ccagggcacg ggcagcttgc
      901 cggtggtgca gatgaacttc agggtcagct tgccgtaggt ggcatcgccc tcgccctcgc
      961 cggacacgct gaacttgtgg ccgtttacgt cgccgtccag ctcgaccagg atgggcacca
     1021 ccccggtgaa cagctcctcg cccttgctca ccatggtggc gaccggtgga tccttgctag
     1081 cagcttgaat tcgatctgcg ttctacggtg gtcagaccga agactgcgac ggtaccgacg
     1141 ctggtcgcgc ctcttatacc cacgtagaac gcagctcagc caatagaatg agtgccaata
     1201 tggaatttcc aggggaaaac cggggcggtg ttacgttttg gctgcccttt cacttcccat
     1261 tgacgtgtat tggctcgaga acggtacttt cccattaatc agctatggga aagtaccgtt
     1321 taaaggtcac gttgcattag tttcaatagc ccattgacgt caatggtggg aaagtacatg
     1381 gcgttttaat taaatggctg gaaaaaccca atgactcacc cctattgacc ttatgtacgt
     1441 gccaataatg ggaaaaaccc attgactcac cccctattga ccttttgtac tgggcaaaac
     1501 ccaatggaaa gtccctattg actcagtgta cttggctcca atgggacttt cctgttgatt
     1561 cacccctatt gaccttatgt actgggcaaa acccattgga aagtccctaa tgactcagta
     1621 tatctctaga actagtggat cccccgggct gcaggaattc gaacgctgac gtcatcaacc
     1681 cgctccaagg aatcgcgggc ccagtgtcac taggcgggaa cacccagcgc gcgtgcgccc
     1741 tggcaggaag atggctgtga gggacagggg agtggcgccc tgcaatattt gcatgtcgct
     1801 atgtgttctg ggaaatcacc ataaacgtga aatgtctttg gatttgggaa tcttataagt
     1861 tctgtatgag accacagatc tcccaatgta tgggatggcg gatagtaagt ttgtaattac
     1921 tgtggcccct cagaatgata tttggcctca cgggaggaca tagcctatga aggctgttgc
     1981 tatagttgca agcaggagga taatgccaag ctttagtttc ttcatctgta aaatagaaat
     2041 gataatggtg tatacgtcat aggactgttg tgagatttaa atgattcatg catgaaattt
     2101 cttagaagca tgcctcacat tatagcattc atttcactaa gtgctatttt tctttccttt
     2161 tgactatgcc cacggcatgc aaaaattcca gggccaggga tcaaacccac ctgtgccaca
     2221 gaagtgggaa tgctggatcc ttaatccggg agccaccaga aaacaccagt gttgactatt
     2281 atcattacac accagccagt tgtatttgta tatgactgat ttctagaatg atggaatctc
     2341 acatcataat aacctgagga tttacttgtt acctcaagtg ggaagttcaa ggatgcacct
     2401 gattctttgg gaggctccat cagtggggcc aggggttctc tgagttttct gtcaggctcc
     2461 ctccaggtgg taaaatggca gctggcagcc tcatatcaac atcccccgat caattcctgt
     2521 ggacaacaac catcttttcc acctactgtg gcaaaaagta accaaggaag acactgattg
     2581 acccagtgag gggcacatac caatccctgt ggcttgaggg tggagaggtg acaggtcagc
     2641 tttattgtat tcacaaccag gaagcagaga agggaacaac aaatgaccac tatagttcct
     2701 acgccctttc tatatgctgc agctgagtcc atctggcttc ctgtttccat tgcaaaggcc
     2761 cactgagttt tcagctcacc atcctctctg acagtccaag tcagagcctg acataattgg
     2821 cccccattct ataatgaatg caccataatt agcctaggtt agtaaaatgc aacaagtaaa
     2881 atacactcag tgcggcaatt tttataaatc tttatcccta tggctatttc aaattcagaa
     2941 gaccagacta gtccaataac taaaagcccc acacatatgt ggtaaaacca tcctctgata
     3001 gatgtaacat tacacagtgg ggccctgcag tccacagtga aagagaaata ggtgaggcca
     3061 gaacatacac tttcttgttt cagctctact aatagttcaa gaaaataaag tctcttagtc
     3121 tcacagatac taaagatccc gtatttctaa aacagaggtt gaagcatcca ggtaaccgac
     3181 tacatggaat ttttgtgaag agtaaaatga actgtcatat gtagaagaga ttagtaaata
     3241 taataaaaat aattagcaat attatctcta ataattaaat tgttctttgt ttcctaatcc
     3301 tcttaaacac agaaatgatt tcttctcttt ggcttagatt tctcttctgt tctcagaata
     3361 gttgtcactt gacaccctgt tgtcacctct tatacaaaga actccacctt ctcttgtctt
     3421 ttctgaaaca accatgcatt tgctttctct atttgtcaca ctcatctccc ttttttctct
     3481 cttagatttt tttttgtttc cattttaatt ggtcattatc ccctcactgg tgataaaagc
     3541 ccacccttct tttttattca ccatggaacc actgtagtat gctggttgga ccattcaaat
     3601 cttgacttca ccttggacct ttcacttgat tctgtctatt caccatcgtt gcacaggaga
     3661 gttctgtctg tgttttgttt ctgagtctgg caccactctg aaggtctaac tttgtaggga
     3721 gtctctctcc agataattta aaaagttgaa ttatccctag gaagtagctc aagaacttga
     3781 atagcctctc aaattccact cactggatta atgcttaaaa aaataatttt tgtggctcag
     3841 tggaattgaa tctgactagt atccatgagg attcaggttc aatccctagc cttgctcagt
     3901 gggtccagga tccggtgttg ctgtgagcta tagtgtaggt tgcagacaca gcctggattc
     3961 tgtgttgctg tggtataggc tggcagctat agcactgatt caaccctcta ggaggaactt
     4021 tcatatgtcg tgtgtgtggc cccccaaaaa taacaacaac gacaactaaa acaataaagt
     4081 aaaatgtgct ttaataaaaa taataaataa ggcattagcc tgaggagctg atggcaccac
     4141 ctgccttcag tacaaggatt caacaccaac gtgtgggtgt ttgggaagag ttgatcagac
     4201 cgacactgaa gctggaaata agcagcagca gcagtagtac cacaaagcct gggaaacaca
     4261 cccacaaaat gacagtgtgc ggaccgagcg gccgcaggaa cccctagtga tggagttggc
     4321 cactccctct ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg
     4381 cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc tgcaggggcg
     4441 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag
     4501 caaccatagt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     4561 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     4621 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     4681 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca
     4741 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     4801 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc gggctattct
     4861 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     4921 caaaaattta acgcgaattt taacaaaata ttaacgttta caattttatg gtgcactctc
     4981 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct
     5041 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     5101 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag
     5161 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     5221 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     5281 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     5341 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     5401 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     5461 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     5521 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     5581 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     5641 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     5701 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     5761 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     5821 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     5881 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     5941 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     6001 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     6061 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     6121 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     6181 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     6241 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     6301 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     6361 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     6421 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     6481 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     6541 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     6601 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     6661 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     6721 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     6781 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     6841 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     6901 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     6961 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     7021 tttgctcaca tgtcctgcag gcagctgcgc gctcgctcgc tcactgaggc cgcccgggca
     7081 aagcccgggc gtcgggcgac ctttggtcgc ccggcctcag tgagcgagcg agcgcgcaga
     7141 gagggagtgg ccaactccat cactaggggt tcctgcggcc gcacgcg
//
