LOCUS       V009689                 9583 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009689
VERSION     V009689
KEYWORDS    complete genome
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9583)
  AUTHORS   Ahmed A.
  TITLE     A rapid procedure for DNA sequencing using transposon-promoted
            deletions in Escherichia coli
  JOURNAL   Gene 39 (2-3), 305-310 (1985)
   PUBMED   3005115
REFERENCE   2  (bases 1 to 9583)
  AUTHORS   Ahmed A.
  TITLE     Sequencing with transposon-promoted deletions: Use of
            transposon-promoted deletions in DNA sequence analysis
  JOURNAL   (in) Wu,R. and Grossman,L. (Eds.); METHODS IN ENZYMOLOGY,
            RECOMBINANT DNA, PART E; Academic Press, New York (1986)
REFERENCE   3  (bases 1 to 9583)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9583)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene 39
            (2-3), 305-310 (1985)"
            SGRef: number: 2; type: "Journal Article"; journalName: "(in) Wu,R.
            and Grossman,L. (Eds.); METHODS IN ENZYMOLOGY, RECOMBINANT DNA, PART
            E; Academic Press, New York (1986)"
            SGRef: number: 3; type: "Journal Article"
            [2]  revises [1].
            Sequence in computer readable form and reprints for [1],[(in) Wu,R.
            and Grossman,L., (Eds.);Methods in Enzymology, Recombinant DNA, part
            ] and [2]
            kindly provided by A.Ahmed, 31-MAR-1986.
            [1] describes a new quick sequencing method, which involves the
            subdivision of the DNA fragment to be sequenced into many
            overlapping segments by a series of deletions which start from a
            fixed site.  Since the different segments retain their natural
            connectedness, the complete sequence is easily assembled from
            overlaps that occur between the sequences of adjacent deletions.  A
            simple and rapid method for the isolation of transposon-promoted
            deletions and their use in sequencing long DNA fragments was
            developed.
FEATURES             Location/Qualifiers
     source          1..9583
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(79..1224)
                     /gene="galK"
                     /label="Galactokinase"
                     /note="Galactokinase from Escherichia coli O157:H7 (strain
                     EC4115 / EHEC). Accession#: B5YRF5"
     CDS             2927..4114
                     /label="TcR"
                     /note="tetracycline efflux protein"
     CDS             4758..4946
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    5051..5191
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(5377..5965)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6139..6996)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6997..7101)
                     /label="AmpR promoter"
     mobile_element  complement(7122..7889)
                     /label="IS1"
                     /note="prokaryotic transposable element"
     promoter        8010..8112
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             8113..8769
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
ORIGIN
        1 ggccttttga aaagcaagca taaaagatca acagtcggta cggctgacca tcgggtgcca
       61 gtgcgggagt ttcgttcagc actgtcctgc tccttgtgat ggtttacaaa cgtaaaaagt
      121 ctctttaata cctgtttttg cttcatattg ttcagcgaca cgttgctgta cggcaggcac
      181 cagctcttcc gggatcagcg cgacgataca gccgccaaat ccgccgccgg tcatgcgtac
      241 gccacctttg tcgccaatca cagctttgac gatttctacc agagtgtcaa tttgcggcac
      301 ggtgatttcg aaatcatcgc gcatagaggc atgagactcc gccatcaact cgcccatacg
      361 tttcaggtcg ccttgctcca gcgcgctggc agcttcaacg gtgcgggcgt tttcagtcag
      421 tatatgacgc acgcgttttg ccacgatcgg gtccagttca tgcgcaacag cgttgaactc
      481 ttcaatggtg acatcacgca gggctggctg ctggaagaaa cgcgcaccgg tttcgcactg
      541 ttcacgacgg gtgttgtatt cgctgccaac cagggtacgt ttgaagttac tgttgatgat
      601 gacgacagcc acacctttgg gcatggaaac tgctttggtc cccagtgagc ggcaatcgat
      661 cagcaaggca tgatctttct tgccgagcgc ggaaattagc tgatccatga tcccgcagtt
      721 acagcctaca aactggtttt ctgcttcctg accgttaagc gcgatttgtg cgccgtccag
      781 cggcagatga taaagctgct gcaatacggt tccgaccgcg acttccagtg aagcggaaga
      841 acttaacccg gcaccctgcg gcacattgcc gctgatcacc atgtccacgc cgccgaagct
      901 gttgttacgc agttgcagat gtttcaccac gccacgaacg tagttagccc attgatagtt
      961 ttcatgtgcg acaatgggcg catcgaggga aaactcgtcg agctgatttt cataatcggc
     1021 tgccatcacg cgaactttac ggtcatcgcg tggtgcacaa ctgatcacgg tttgataatc
     1081 aatcgcgcag ggcagaacga aaccgtcgtt gtagtcggtg tgttcaccaa tcaaattcac
     1141 gcggccaggc gcctgaatgg tgtgagtggc agggtagcca aatgcgttgg caaacagaga
     1201 ttgtgttttt tctttcagac tcatttctta cactccggat tcgcgaaaat ggatatcgct
     1261 gactgcgcgc aaacgctctg ctgcctgttc tgcggtcagg tctcgctggg tctctgccag
     1321 catttcataa ccaaccataa atttacgtac ggtggcggac gcagcagagg cgataaaagt
     1381 gcgcgtgcag ctgccagtgt tgattctctt cgccattaaa tgggcgccgt gccagcccat
     1441 agagtagggg aaggagcact ggaagaggtt gtcataacga ctggtcagct ttttcaacgc
     1501 cagcgccaga tcgctgcgct gggcgtcggt caaatcggtg atccgtaaaa cgtgggcttt
     1561 gggcagcagt agcgtttcga acggccaggc agcccagtaa ggcacgacgg ctaaccagtg
     1621 ttcggtttcg acaacggtac ggctaccgtc tgccagctcg cgctgaacat aatccaccag
     1681 cattggtgat ttctgttcgg caaaatattc tttttgcagg cggtcttcgc gctcagcttc
     1741 gttaggcagg aagctatttg cccaaatctg accgtgcgga tgcgggttag agcagcccat
     1801 cgccgcgcct ttgttttcaa aaacctgcac ccatgggtac gttttcccca gttctgcggt
     1861 ttgctcctgc caggttttga cgatttccgt caatgctgca acgctgagct ctggcagcgt
     1921 tttactgtga tccggtgaaa agcagatcac ccggctggtg ccgcgcgcgc tctggcaacg
     1981 catcagcgga tcgtgacttt ctggcgcatc tggcgtgtca gacatcaaag ccgcaaagtc
     2041 attagtgaaa acgtaagtcc cggtgtaatc ggggttttta tcgcctgtca cccgcacatt
     2101 acctgcgcag aggaagcaat ctggatcgtg cgcaggtaac acctgtttgg ctggcgtttc
     2161 ctgcgccccc tgccagggcg ttagccgggt gcggtgaaac cagaatccat tgcccggtga
     2221 gcggttgtag cggcgatgtg gatgatcaac gggattaaat tgcgtcatgg tcgttcctta
     2281 atcgggatat ccctgtggat ggcgtgactg ccagtgccag gtgtcctgcg ccatttcatc
     2341 gagtgtgcgc gttacgcagt tcagttcacg gtcggctttg ctggcgtccg ccagtaggcc
     2401 ggaaggtcgc cctcgcgagc ggtgcaaaat gataattaac cggtttgccg cacgtttgct
     2461 gaaggcatta accacgtcca gcacgctgtt gcctacgcca gcgccgaggt tgtagatgtg
     2521 tacgcctggc ttgttcgcca gtttttccat cgccacgacg tgaccgtccg ccagatccat
     2581 tacgtggatg taatcgcgta cgccagtacc atcttcggtc ggataatcgt taccaaaaat
     2641 cgccagcgag tcgcgacggc ctacagcaac ctgggcgatg tatggcatca ggttattcgg
     2701 aatgccttgc ggatcttcgc ccatatcgcc cgacggatgc gcgccaaccg ggttgaagta
     2761 gcgcacgagg gcaatgctcc agtccggctg ggctttttgc agatcggtga ggatctgttc
     2821 caccatcagc ttgcttttgc cgtaagggct ttgcggtgtg ccggtcggga agctataatg
     2881 cggtagttta tcacagttaa attgctaacg cagtcaggca ccgtgtatga aatctaacaa
     2941 tgcgctcatc gtcatcctcg gcaccgtcac cctggatgct gtaggcatag gcttggttat
     3001 gccggtactg ccgggcctct tgcgggatat cgtccattcc gacagcatcg ccagtcacta
     3061 tggcgtgctg ctagcgctat atgcgttgat gcaatttcta tgcgcacccg ttctcggagc
     3121 actgtccgac cgctttggcc gccgcccagt cctgctcgct tcgctacttg gagccactat
     3181 cgactacgcg atcatggcga ccacacccgt cctgtggatc ctctacgccg gacgcatcgt
     3241 ggccggcatc accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga
     3301 tggggaagat cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt
     3361 ggcaggcccc gtggccgggg gactgttggg cgccatctcc ttgcatgcac cattccttgc
     3421 ggcggcggtg ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca
     3481 taagggagag cgtcgaccga tgcccttgag agccttcaac ccagtcagct ccttccggtg
     3541 ggcgcggggc atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt
     3601 aggacaggtg ccggcagcgc tctgggtcat tttcggcgag gaccgctttc gctggagcgc
     3661 gacgatgatc ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg ctcaagcctt
     3721 cgtcactggt cccgccacca aacgtttcgg cgagaagcag gccattatcg ccggcatggc
     3781 ggccgacgcg ctgggctacg tcttgctggc gttcgcgacg cgaggctgga tggccttccc
     3841 cattatgatt cttctcgctt ccggcggcat cgggatgccc gcgttgcagg ccatgctgtc
     3901 caggcaggta gatgacgacc atcagggaca gcttcaagga tcgctcgcgg ctcttaccag
     3961 cctaacttcg atcattggac cgctgatcgt cacggcgatt tatgccgcct cggcgagcac
     4021 atggaacggg ttggcatgga ttgtaggcgc cgccctatac cttgtctgcc tccccgcgtt
     4081 gcgtcgcggt gcatggagcc gggccacctc gacctgaatg gaagccggcg gcacctcgct
     4141 aacggattca ccactccaag aattggagcc aatcaattct tgcggagaac tgtgaatgcg
     4201 caaaccaacc cttggcagaa catatccatc gcgtccgcca tctccagcag ccgcacgcgg
     4261 cgcatctcgg gcagcgttgg gtcctggcca cgggtgcgca tgatcgtgct cctgtcgttg
     4321 aggacccggc taggctggcg gggttgcctt actggttagc agaatgaatc accgatacgc
     4381 gagcgaacgt gaagcgactg ctgctgcaaa acgtctgcga cctgagcaac aacatgaatg
     4441 gtcttcggtt tccgtgtttc gtaaagtctg gaaacgcgga agtcagcgcc ctgcaccatt
     4501 atgttccgga tctgcatcgc aggatgctgc tggctaccct gtggaacacc tacatctgta
     4561 ttaacgaagc gctggcattg accctgagtg atttttctct ggtcccgccg catccatacc
     4621 gccagttgtt taccctcaca acgttccagt aaccgggcat gttcatcatc agtaacccgt
     4681 atcgtgagca tcctctctcg tttcatcggt atcattaccc ccatgaacag aaattccccc
     4741 ttacacggag gcatcaagtg accaaacagg aaaaaaccgc ccttaacatg gcccgcttta
     4801 tcagaagcca gacattaacg cttctggaga aactcaacga gctggacgcg gatgaacagg
     4861 cagacatctg tgaatcgctt cacgaccacg ctgatgagct ttaccgcagc tgcctcgcgc
     4921 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     4981 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     5041 ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat actggcttaa
     5101 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
     5161 cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc
     5221 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     5281 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     5341 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     5401 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     5461 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     5521 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg
     5581 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     5641 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     5701 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     5761 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     5821 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     5881 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     5941 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     6001 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     6061 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     6121 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     6181 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     6241 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     6301 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     6361 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     6421 taatagtttg cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt
     6481 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     6541 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     6601 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     6661 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     6721 gcggcgaccg agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag
     6781 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     6841 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     6901 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     6961 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     7021 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     7081 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg cggtaatgac tccaacttat
     7141 tgatagtgtt ttatgttcag ataatgcccg atgactttgt catgcagctc caccgatttt
     7201 gagaacgaca gcgacttccg tcccagccgt gccaggtgct gcctcagatt caggttatgc
     7261 cgctcaattc gctgcgtata tcgcttgctg attacgtgca gctttccctt caggcgggat
     7321 tcatacagcg gccagccatc cgtcatccat atcaccacgt caaagggtga cagcaggctc
     7381 ataagacgcc ccagcgtcgc catagtgcgt tcaccgaata cgtgcgcaac aaccgtcttc
     7441 cggagactgt catacgcgta aaacagccag cgctggcgcg atttagcccc gacatagccc
     7501 cactgttcgt ccatttccgc gcagacgatg acgtcactgc ccggctgtat gcgcgaggtt
     7561 accgactgcg gcctgagttt tttaagtgac gtaaaatcgt gttgaggcca acgcccataa
     7621 tgcgggctgt tgcccggcat ccaacgccat tcatggccat atcaatgatt ttctggtgcg
     7681 taccgggttg agaagcggtg taagtgaact gcagttgcca tgttttacgg cagtgagagc
     7741 agagatagcg ctgatgtccg gcggtgcttt tgccgttacg caccaccccg tcagtagctg
     7801 aacaggaggg acagctgata gaaacagaag ccactggagc acctcaaaaa caccatcata
     7861 cactaaatca gtaagttggc agcatcaccc gacgcacttt gcgccgaata aatacctgtg
     7921 acggaagatc acttcgcaga ataaataaat cctggtgtcc ctgttgatac cgggaagccc
     7981 tgggccaact tttggcgaaa atgagacgtt gatcggcacg taagaggttc caactttcac
     8041 cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt ttcaggagct
     8101 aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg
     8161 catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc
     8221 gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat
     8281 ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt ccgtatggca
     8341 atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat
     8401 gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt
     8461 ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa
     8521 gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt caccagtttt
     8581 gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat
     8641 tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtctgt
     8701 gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag
     8761 ggcggggcgt aattttttta aggcagttat tggtgccctt aaacgcctgg tgctacgcct
     8821 gaataagtga taataagcgg atgaatggca gaaattcgaa agcaaattcg acccggtcgt
     8881 cggttcaggg cagggtcgtt aaatagccgc ttatgtctat tgctggttta ccggtttatt
     8941 gactaccgga agcagtgtga ccgtgtgctt ctcaaatgcc tgaggccagt tggtaatgac
     9001 tccaacttat tgatagtgtt ttatgttcag ataatgcccg atgactttgt catgcagctc
     9061 caccgatttt gagaacgaca gcgacttccg tcccagccgt gccaggtgct gcctcagatt
     9121 caggttatgc cgctcaattc gctgcgtata tcgcttgctg attacgtgca gctttccctt
     9181 caggcgggat tcatacagcg gccagccatc cgtcatccat atcaccacgt caaagggtga
     9241 cagcaggctc ataagacgcc ccagcgtcgc catagtgcgt tcaccgaata cgtgcgcaac
     9301 aaccgtcttc cggagactgt catacgcgta aaacagccag cgctggcgcg atttagcccc
     9361 gacatagccc cactgttcgt ccatttccgc gcagacgatg acgtcactgc ccggctgtat
     9421 gcgcgaggtt accgactgcg gcctgagttt tttaagtgac gtaaaatcgt gttgaggcca
     9481 acgcccataa tgcgggctgt tgcccggcat ccaacgccat tcatggccat atcaatgatt
     9541 ttctggtgcg taccgggttg agaagcggtg taagtgaact gca
//
