LOCUS       40924_2987        5143 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Phagemid cloning vector pA2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5143)
  AUTHORS   Fagerlund A, Myrset AH, Kulseth MA.
  TITLE     Construction and characterization of a 9-mer phage display 
            pVIII-library with regulated peptide density
  JOURNAL   Appl. Microbiol. Biotechnol. 80 (5), 925-936 (2008)
  PUBMED    18716770
REFERENCE   2  (bases 1 to 5143)
  AUTHORS   Fagerlund A, Myrset AH, Kulseth MA.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-FEB-2008) GE Healthcare Medical Diagnostics, P.O. Box 
            4220 Nydalen, Oslo N-0401, Norway
REFERENCE   3  (bases 1 to 5143)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5143)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Microbiol. Biotechnol."; date: "2008"; volume: "80"; issue: "5"; 
            pages: "925-936"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-FEB-2008) GE Healthcare Medical Diagnostics, P.O. Box 4220 
            Nydalen, Oslo N-0401, Norway"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5143
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..285
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     gene            318..891
                     /gene="gVIII"
                     /label=gVIII
     CDS             318..401
                     /codon_start=1
                     /gene="gVIII"
                     /product="major coat protein pVIII"
                     /label=gVIII
                     /protein_id="ACA60871.1"
                     /translation="MKKSLVLKASVAVATLVPMLSFAAEGEF"
     sig_peptide     318..398
                     /gene="gVIII"
     misc_feature    396..401
                     /gene="gVIII"
                     /label=unique EcoRI cloning site
                     /note="unique EcoRI cloning site"
     misc_feature    402..749
                     /gene="gVIII"
                     /note="spacer fragment; derived from lambda phage"
     misc_feature    750..755
                     /gene="gVIII"
                     /label=unique BglII cloning site
                     /note="unique BglII cloning site"
     CDS             751..891
                     /codon_start=1
                     /gene="gVIII"
                     /product="major coat protein pVIII"
                     /label=gVIII
                     /protein_id="ACA60872.1"
                     /translation="DLAKAAFDSLQASATEYIGYAWAMVVVIVGATIGIKLFKKFTSKA
                     S"
     primer_bind     complement(891..907)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      1348..1861
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        1925..2029
                     /label=AmpR promoter
     CDS             2030..2887
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      3061..3649
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(3835..3975)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(4242..5117)
                     /label=araC
                     /note="L-arabinose regulatory protein"
ORIGIN
        1 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct
       61 tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca
      121 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg
      181 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg
      241 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg ttttttgggc
      301 taacaggagg aattaacatg aaaaagtctt tagtcctcaa agcctctgta gccgttgcta
      361 ccctcgttcc gatgctgtct ttcgctgctg agggtgaatt ctctgacgaa taatcttttc
      421 ttttttcttt tgtaatagtg tcttttgtgt ccccctgttt tgagggatag caatccccca
      481 atttgaggga tgttttatcc ctcgttttag gggattttcc ctcgttttga gggatgcacc
      541 attctgagat gtttttattt ggtccaaaca tgccgccttg ctgcttgata atattcattc
      601 tgacgagttc taacttggct tcattgcacc gtttgacagg taactttgta atctcgctaa
      661 gttgagaatc ggtgattctg tccattggtt tattccaccc ataggtttta cgcagaatgg
      721 caagcagcac tttaaactgt cgcttggtca gatctcgcaa aagcggcctt tgactccctg
      781 caagcctcag cgaccgaata tatcggttat gcgtgggcga tggttgttgt cattgtcggc
      841 gcaactatcg gtatcaagct gtttaagaaa ttcacctcga aagcaagcta actggccgtc
      901 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
      961 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     1021 cagttgcgca gcctgaatgg cgaatggcga ttccgttgca atggctggcg gtaatattgt
     1081 tctggatatt accagcaagg ccgatagttt gagttcttct actcaggcaa gtgatgttat
     1141 tactaatcaa agaagtattg cgacaacggt taatttgcgt gatggacaga ctcttttact
     1201 cggtggcctc actgattata aaaacacttc tcaggattct ggcgtaccgt tcctgtctaa
     1261 aatcccttta atcggcctcc tgtttagctc ccgctctgat tctaacgagg aaagcacgtt
     1321 atacgtgctc gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg
     1381 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     1441 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     1501 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     1561 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     1621 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     1681 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     1741 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa
     1801 tttaaatatt tgcttataca atcttcctgt ttttggggct tttctgatta tcaaccgggg
     1861 tacatatgat tgacatgcta gttttacgat taccgttcat ggtggcactt ttcggggaaa
     1921 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatct agacgctcat
     1981 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     2041 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     2101 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     2161 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     2221 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc
     2281 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     2341 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     2401 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     2461 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     2521 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     2581 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     2641 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     2701 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     2761 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     2821 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     2881 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     2941 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     3001 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     3061 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     3121 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     3181 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
     3241 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     3301 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     3361 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     3421 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     3481 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     3541 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     3601 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     3661 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     3721 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     3781 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
     3841 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag
     3901 tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac
     3961 tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
     4021 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     4081 aggttttcac cgtcatcacc gaaacgcgcg aggcagcaga tcaattcgcg cgcgaaggcg
     4141 aagcggcatg cataatgtgc ctgtcaaatg gacgaagcag ggattctgca aaccctatgc
     4201 tactccgtca agccgtcaat tgtctgattc gttaccaatt atgacaactt gacggctaca
     4261 tcattcactt tttcttcaca accggcacgg aactcgctcg ggctggcccc ggtgcatttt
     4321 ttaaataccc gcgagaaata gagttgatcg tcaaaaccaa cattgcgacc gacggtggcg
     4381 ataggcatcc gggtggtgct caaaagcagc ttcgcctggc tgatacgttg gtcctcgcgc
     4441 cagcttaaga cgctaatccc taactgctgg cggaaaagat gtgacagacg cgacggcgac
     4501 aagcaaacat gctgtgcgac gctggcgata tcaaaattgc tgtctgccag gtgatcgctg
     4561 atgtactgac aagcctcgcg tacccgatta tccatcggtg gatggagcga ctcgttaatc
     4621 gcttccatgc gccgcagtaa caattgctca agcagattta tcgccagcag ctccgaatag
     4681 cgcccttccc cttgcccggc gttaatgatt tgcccaaaca ggtcgctgaa atgcggctgg
     4741 tgcgcttcat ccgggcgaaa gaaccccgta ttggcaaata ttgacggcca gttaagccat
     4801 tcatgccagt aggcgcgcgg acgaaagtaa acccactggt gataccattc gcgagcctcc
     4861 ggatgacgac cgtagtgatg aatctctcct ggcgggaaca gcaaaatatc acccggtcgg
     4921 caaacaaatt ctcgtccctg atttttcacc accccctgac cgcgaatggt gagattgaga
     4981 atataacctt tcattcccag cggtcggtcg ataaaaaaat cgagataacc gttggcctca
     5041 atcggcgtta aacccgccac cagatgggca ttaaacgagt atcccggcag caggggatca
     5101 ttttgcgctt cagccatact tttcatactc ccgccattca gag
//
